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The map label for this gene is paaI [H]

Identifier: 221232588

GI number: 221232588

Start: 1813158

End: 1813562

Strand: Reverse

Name: paaI [H]

Synonym: SPN23F_18660

Alternate gene names: 221232588

Gene position: 1813562-1813158 (Counterclockwise)

Preceding gene: 221232589

Following gene: 221232586

Centisome position: 81.64

GC content: 43.7

Gene sequence:

>405_bases
ATGAAAGACTTTCATTTTGACGCTATATCTGCCTTTGAAAATTACGAAATTGAAAAAATGAGAGATGGTCACGTTGTGGT
GACGACCAAAGTAGTGAACTCGTCGCTCAACTACTATGGCAATGCCCATGGTGGCTATCTATTCACGCTATGTGACCAAA
TCAGTGGTTTGGTGGTTATCTCGCTGGGACTTGATGGGGTGACACTCCAATCCTCTATCAACTACCTTAAGGCAGGAAAA
CTCGACGATGTATTGACCATTAAAGGAGAATGTGTCCATCAAGGCCGTACAACCTGTGTGATGGATGTGGATATCACCAA
TCAAGAGGGCAGAAATGTCTGTAAAGCAACCTTTACCATGTTTGTCACAGGCCAGCGGTCAGAAGAAAGACAGGTAAGGA
TATAG

Upstream 100 bases:

>100_bases
TGTGGAACATGTCGGAATTTTACTAGACTAGGAGCTTTCTCCTTGCACAGTCCTATAAAAAGTAGTATCATAGTGTCGTT
TGAAATATCAGGAGGAAACG

Downstream 100 bases:

>100_bases
ATAAACAAAAAAGAGGCTTACACCTCTTTTTTAGTTTCAGATAAATCAATCAATTTCTTTAATAAACTTAGCAATTTCCT
TTACAATCTCAGTAACCAAA

Product: thioesterase superfamily protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGK
LDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERQVRI

Sequences:

>Translated_134_residues
MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGK
LDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERQVRI
>Mature_134_residues
MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGK
LDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERQVRI

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=107, Percent_Identity=35.5140186915888, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 14917; Mature: 14917

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVI
CCCCCCHHHHHHCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEE
SLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTM
EECCCCEEECCCCCCCCCCCCCCEEEECCEEEECCCEEEEEEEEECCCCCCHHEEEEEEE
FVTGQRSEERQVRI
EEECCCCCCCCCCC
>Mature Secondary Structure
MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVI
CCCCCCHHHHHHCCCCEEEECCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEE
SLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTM
EECCCCEEECCCCCCCCCCCCCCEEEECCEEEECCCEEEEEEEEECCCCCCHHEEEEEEE
FVTGQRSEERQVRI
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]