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The map label for this gene is sau3AIR [H]

Identifier: 221232586

GI number: 221232586

Start: 1810240

End: 1811730

Strand: Reverse

Name: sau3AIR [H]

Synonym: SPN23F_18640

Alternate gene names: 221232586

Gene position: 1811730-1810240 (Counterclockwise)

Preceding gene: 221232588

Following gene: 221232581

Centisome position: 81.56

GC content: 29.91

Gene sequence:

>1491_bases
ATGGATCAAAACTTATTTAACTATAACGATGAAGATATCGATAGTGTTATTGAATATTCTCATAAACTATTAAATAGAAA
ATTTTCCGATGTTATGGAGGAGTATAATCGTTCGCTTTATAAATCATATGACGATTATAATGATAGAGTAGTCTCTGAGG
TACAAGATAAAGCAATTAGTATGAAGTCAAAAGGGCAGTATGGAAATTATATTGAAAAATATTTCTATGGTTATCAGCCA
AATAGTGACTCGGAAGCTGACTTTGAAAAAATAGGTGTAGAATTAAAAGTTACTCCTTTTAAGATAAACAAAAATGGGAC
ACTTAGTGCAAAAGAACGACTTGTATTAACAATACTAAACTATATGGAGGAAAATTTAGAGGATTTTTACTCCACTCATT
TATGGAAAAAATGTGCAAAGATTTTGTTATTGTTTTACAATGGCTTAATTCCTAATCAAACAATGAAGGACTATGTCATT
GAAAAAATATTTTTATATGAATGGTTTGAGGAAGATATGGCAGTCATCCTTGAAGACTATCAGAAGATTACAGATAAAAT
CAAAAATGGAAAGGCACATGAATTATCAGAATCAGATGGAAACTATTTATCAACTTGTACAAAAGGGGCAGGTAAAGGAA
AGGATTTAAGACAACAACCTTTTAGTCATGAATTAGCAAAACAAAGAGCTTGGGAATTAAAATCAAGTTATATGACCTAT
CTGATAAATCATAAAATCTTTAATCAATCAGATCAAGAAAGTGTTCTGGCTAATTTTAGGGGTGAGAAAAAATCATTTAC
TGAGGTTATTGCAGAGAAGATTCTTTCCTACAAAGGTTTTTCTGAACAAGAGTTATATGATAGATTTGAAGTGAATTCCA
AAGCAAAAGGTAAGAATAGTACTCTGATTAGAAAAATTTTAGGCTTAACAGGGGATTTAGATAAGACAAAAGAATTTCAA
AAAGCAAACATGAATTTGAGAGTTATTCGAGTAGATAAGAATAATTTACCTAAGGAAGACTCTCCATTTAAAACTTATTG
TTTTAAAGAACTAGCAGCAACAGATAGTTGGGAATCATCACATGTTTATAATGAAATTTATAATAAACGATTCCTTTTCG
TTATTTTTAAGGAAATTGAACCTAAGCTATTTGTATTAGATTCTATCAAGTTTTGGGGATTTCAGGATAGACAATTAGAA
GAAATCCAAAGAGTATGGCAGGAAACTAGACAAATTATTTCTGACGGAGTTAAATTAACTCAAAACGGTAATAAAGTTTC
AACTAATTTCCCTCAAAGTAAGATAAACAAAATTCTATTTACGAAACTACATGCTACCAATACTTATTATGAAATTGACA
AAGGAAAATTTGTTGGGAAAGGTTCATTATCTGATACGGATGAACTTCCAGATGGACGTAGGATTACAAAACACTCCTTT
TGGATGCCTAAGAAATTTATTAAAGAAATATTAGATGGGAATTGGGATTGA

Upstream 100 bases:

>100_bases
TTTTACAGTTTAATCCATTATATACTATTTTCAATGATTGATCAAGTATTAATGTTTGCTTTAGTTTAATTTGATATAAT
ATAAAAGATAGGAGAAGAAA

Downstream 100 bases:

>100_bases
TTGTATTTATAATCAATTTGTGATAAACAAAAAAGAGGCTCGCACCTCTTTTTCTTATTTCTTTTTATGATTTAATACGG
CATTGAGGACAATAGTGAGT

Product: restriction enzyme

Products: NA

Alternate protein names: R.Sau3AI; Endonuclease Sau3AI; Type II restriction enzyme Sau3AI [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAISMKSKGQYGNYIEKYFYGYQP
NSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTILNYMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVI
EKIFLYEWFEEDMAVILEDYQKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTY
LINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNSTLIRKILGLTGDLDKTKEFQ
KANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESSHVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLE
EIQRVWQETRQIISDGVKLTQNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSF
WMPKKFIKEILDGNWD

Sequences:

>Translated_496_residues
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAISMKSKGQYGNYIEKYFYGYQP
NSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTILNYMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVI
EKIFLYEWFEEDMAVILEDYQKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTY
LINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNSTLIRKILGLTGDLDKTKEFQ
KANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESSHVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLE
EIQRVWQETRQIISDGVKLTQNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSF
WMPKKFIKEILDGNWD
>Mature_496_residues
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAISMKSKGQYGNYIEKYFYGYQP
NSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTILNYMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVI
EKIFLYEWFEEDMAVILEDYQKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTY
LINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNSTLIRKILGLTGDLDKTKEFQ
KANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESSHVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLE
EIQRVWQETRQIISDGVKLTQNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSF
WMPKKFIKEILDGNWD

Specific function: Recognizes the double-stranded sequence GATC and cleaves before G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011337
- InterPro:   IPR011335 [H]

Pfam domain/function: PF02976 MutH [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 58355; Mature: 58355

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAIS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MKSKGQYGNYIEKYFYGYQPNSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTILN
HHCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
YMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVIEKIFLYEWFEEDMAVILEDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTY
HHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNS
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
TLIRKILGLTGDLDKTKEFQKANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESS
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
HVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLEEIQRVWQETRQIISDGVKLT
HHHHHHHCCEEEEEEEECCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSF
CCCCEECCCCCHHHHHHHHHHHEECCCCEEEECCCCEEECCCCCCCCCCCCCCEECHHHC
WMPKKFIKEILDGNWD
CCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDQNLFNYNDEDIDSVIEYSHKLLNRKFSDVMEEYNRSLYKSYDDYNDRVVSEVQDKAIS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
MKSKGQYGNYIEKYFYGYQPNSDSEADFEKIGVELKVTPFKINKNGTLSAKERLVLTILN
HHCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHH
YMEENLEDFYSTHLWKKCAKILLLFYNGLIPNQTMKDYVIEKIFLYEWFEEDMAVILEDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKITDKIKNGKAHELSESDGNYLSTCTKGAGKGKDLRQQPFSHELAKQRAWELKSSYMTY
HHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LINHKIFNQSDQESVLANFRGEKKSFTEVIAEKILSYKGFSEQELYDRFEVNSKAKGKNS
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
TLIRKILGLTGDLDKTKEFQKANMNLRVIRVDKNNLPKEDSPFKTYCFKELAATDSWESS
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
HVYNEIYNKRFLFVIFKEIEPKLFVLDSIKFWGFQDRQLEEIQRVWQETRQIISDGVKLT
HHHHHHHCCEEEEEEEECCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
QNGNKVSTNFPQSKINKILFTKLHATNTYYEIDKGKFVGKGSLSDTDELPDGRRITKHSF
CCCCEECCCCCHHHHHHHHHHHEECCCCEEEECCCCEEECCCCCCCCCCCCCCEECHHHC
WMPKKFIKEILDGNWD
CCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2227451 [H]