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The map label for this gene is sacA [H]

Identifier: 221232532

GI number: 221232532

Start: 1753460

End: 1754779

Strand: Reverse

Name: sacA [H]

Synonym: SPN23F_18060

Alternate gene names: 221232532

Gene position: 1754779-1753460 (Counterclockwise)

Preceding gene: 221232533

Following gene: 221232530

Centisome position: 79.0

GC content: 40.08

Gene sequence:

>1320_bases
ATGGGAGATAAGAAATACACAGTAGAAAAAGCCAATCGTTTTATAGCAGAAAATAAACATCTCGTTAATACTCAATATAA
GCCTGAAGAACATTTTTCAGCTGAGATTGGTTGGATCAATGATCCAAATGGATTTGTTTATTTTTGTGGAGAATACCATC
TCTTTTATCAATTCTATCCATATGATAGTGTTTGGGGACCTATGCACTGGGGACATGCTAAAAGTAAGGACTTGGTGACT
TGGGAGCACTTGCCAGTGGCACTTGCTCCTGATCAAGATTATGACCGAAATGGTTGTTTCTCAGGCTCTGCCATTGTCAA
GGATGATCGCCTCTGGCTCATGTACACTGGGCATATCGAAGAAAAAACCGGTGTCCGCCAAGTGCAAAATATGGCATTTT
CAGATGACGGGATTCACTTTGAAAAGATTTCCCAAAATCCAGTTGTAACTGGAACAGACTTGCCAGATGAGTTGATTGCT
GCTGATTTCCGTGATCCAAAACTCTTTGAAAAAGATGGACGCTATTACTCTGTAGTAGCTGCCAAACACAAGGATAATGT
GGGCTGTATCGTTCTACTAGGGTCCGATAACCTAGTAGAATGGCAGTTCGAATCCATCTTTTTAAAAGGGGGAGAACACC
AAGGCTTTATGTGGGAATGCCCAGATTACTTCGAGTTAGATGGGAAAGATTGCCTTATTATGTCACCCATGCGTTATCAG
CGTGAGGGAGACTCATATCATAACATCAACTCATCGCTTTTGTTCACGGGTAAGGTAGATTGGGGAGAAAAACGTTTTAT
CCCAGAATCAGTTCAAGAAATTGATCATGGCCAAGACTTCTATGCGCCTCAAACATTGTTGGACAATCAAAATCGTCGTA
TCCTGATTGCTTGGATGCAGACATGGGGGCGTACCCTTCCAACCCATGACCAAGAACACAAATGGGCATGTGCCATGACT
TTACCTAGAATTCTAAGATTGGAAGATGGCAAACTAAGACAATTCCCTGTTAAAAAAGGCCAATATCAAATCCAAATAGA
TAAAGATTGTCATTACCACTTAGGAAATGATATAGATTATCTTGAATTTGGTTATGACAGTAATGCGCAGCAAGTTTACA
TTGATCGTAGCCATCTTATTCAAAAAATTCTAGGTGAAGAAGAACAGGACACTAGTCGACGGTATGTAGATATTGAAGCT
AAAGAATTGGAAGTTGTTCTAGATAAAAATTCCATCGAGATTTTTGTCAATCAAGGTGAAGCAAGCTTGACTGCAACTTA
TTACTTAACGGTGCCAGCTGAGCTATCACGAATTGATTAA

Upstream 100 bases:

>100_bases
TTACAAGAAAGAACTTGAAAAATACGGACTTTCTGATTACCTCGCTATTAAACAAAAATACTACGATCAATACCAAGCAA
ACAAAAACTAGAGGTTGATT

Downstream 100 bases:

>100_bases
AAATTAAGTTATTTCTCCTAAAGAAAAAGTTCTCTTTCTAAAATAGTGGAAAGAGGACTTTTTGTGTTTTGGGTATATAA
GCTTAGTTTATGGTATTTGT

Product: beta-fructofuranosidase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFCGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVT
WEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
REGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDHGQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMT
LPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEA
KELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID

Sequences:

>Translated_439_residues
MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFCGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVT
WEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
REGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDHGQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMT
LPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEA
KELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID
>Mature_438_residues
GDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFCGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTW
EHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIAA
DFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQR
EGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDHGQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTL
PRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAK
ELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=509, Percent_Identity=25.147347740668, Blast_Score=134, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 51105; Mature: 50974

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFCGEYHLFYQFYP
CCCCEEEHHHHHHHHHCCCEEECCCCCCCHHCCEEECEEECCCCEEEEECCEEEEEEEEC
YDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIE
CCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
EKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIAADFRDPKLFEKDGRYYSVVA
CHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEE
AKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
ECCCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCEEECCCCCEECCCCCEEEECCHHHH
REGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDHGQDFYAPQTLLDNQNRRILIAWMQ
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEH
TWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDIDY
HHCCCCCCCCCCCCEEEEECCCHHEEECCCCEEECCCCCCCEEEEECCCCCEECCCCCCE
LEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAKELEVVLDKNSIEIFVNQGE
EEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEECCC
ASLTATYYLTVPAELSRID
CEEEEEEEEEECHHHCCCC
>Mature Secondary Structure 
GDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNGFVYFCGEYHLFYQFYP
CCCEEEHHHHHHHHHCCCEEECCCCCCCHHCCEEECEEECCCCEEEEECCEEEEEEEEC
YDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSGSAIVKDDRLWLMYTGHIE
CCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCC
EKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIAADFRDPKLFEKDGRYYSVVA
CHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEE
AKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMWECPDYFELDGKDCLIMSPMRYQ
ECCCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCEEECCCCCEECCCCCEEEECCHHHH
REGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDHGQDFYAPQTLLDNQNRRILIAWMQ
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEH
TWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQFPVKKGQYQIQIDKDCHYHLGNDIDY
HHCCCCCCCCCCCCEEEEECCCHHEEECCCCEEECCCCCCCEEEEECCCCCEECCCCCCE
LEFGYDSNAQQVYIDRSHLIQKILGEEEQDTSRRYVDIEAKELEVVLDKNSIEIFVNQGE
EEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEEECCCEEEEEECCC
ASLTATYYLTVPAELSRID
CEEEEEEEEEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]