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The map label for this gene is leuD [H]

Identifier: 221231946

GI number: 221231946

Start: 1125809

End: 1126168

Strand: Reverse

Name: leuD [H]

Synonym: SPN23F_11500

Alternate gene names: 221231946

Gene position: 1126168-1125809 (Counterclockwise)

Preceding gene: 221231947

Following gene: 221231945

Centisome position: 50.7

GC content: 43.33

Gene sequence:

>360_bases
ATGGCAGGGTCTTCGAGGGAACACGCTGCTTGGGTTCTAGCGGACTATGGTTTTAAGGTCGTGATTGCAGGATCTTTCGG
TGACATTCATTACAATAATGAACTCAATAATGGCCTGTTGCCAATCGTTCAGCCTAGAGAGGTTAGAGAGAAACTAGCCC
AGCTAAAACCAACCGACCAGGTAACTGTGGACTTGGAACAACAAAAAATCATCTCACCAGTTGAAGAATTCACCTTCGAG
ATAGATAGCGAGTGGAAACATAAACTCCTAAATAGTTTGGATGATATCGGTATTACCTTGCAGTATGAAGAGTTGATTGC
TGCTTATGAAAAACAACGACCAGCCTACTGGCAGGATTAG

Upstream 100 bases:

>100_bases
CACTTTTCACCACAAGACTCGAAAATGGTACTTTAAAACAGTTTGGTTTCTTGGGATGGTGACCACACTAGTAGCCTTAT
ATTTTATTTGGAGGTAATGG

Downstream 100 bases:

>100_bases
AAAAAATAGAAAAGGAAATATAGTAAACTGAAATAAGATGTAAACAAATGAATTAGAGCTTAACATCCATTTCCAGCAAT
TTTTTAGAAACTACAGTGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQPREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFE
IDSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD

Sequences:

>Translated_119_residues
MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQPREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFE
IDSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD
>Mature_118_residues
AGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQPREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEI
DSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=118, Percent_Identity=50, Blast_Score=114, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321429, Length=117, Percent_Identity=49.5726495726496, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 13676; Mature: 13544

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQPREVREKLAQLKPTDQ
CCCCCCCCEEEEEECCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD
EEEECCHHHHHCHHHHHEECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQPREVREKLAQLKPTDQ
CCCCCCCEEEEEECCCEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQYEELIAAYEKQRPAYWQD
EEEECCHHHHHCHHHHHEECCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]