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The map label for this gene is mcrB [H]

Identifier: 221231945

GI number: 221231945

Start: 1122949

End: 1124862

Strand: Reverse

Name: mcrB [H]

Synonym: SPN23F_11460

Alternate gene names: 221231945

Gene position: 1124862-1122949 (Counterclockwise)

Preceding gene: 221231946

Following gene: 221231944

Centisome position: 50.64

GC content: 32.81

Gene sequence:

>1914_bases
ATGAATAAAAGAAAAGTTAGTTTAGAAGATTTTTATAAATGGTATAGTCTAAATAAAGAAGAGTTATTAAATAAGGCAAC
TGTTGGTGAAAAGTTTAATGATAAATTAAAAGAAGAGTTTCTCCAGGAATGGCCTTTGGATAGGATTTTAACAATGTCAA
TCGATGAATATGTAATAGGAAAGGGACAGCAAAATAAATCTTTATGCTACGCTCTTGAGAAGGGAAAATACAAAAATCTA
TTTCTTGGAATTTCTGGTGGCTCAGCTTCAAAATTTGGTATTTATTGGAATAAAAAAACAAACAAATATAAAGATCAAGC
TAATAATGAGATTTCAGAGTTGGATCAGCGATTTTCAAAATTAAAATCAGATTTGTATGAAATTATCAAAGAAGGTATTC
GTTTTAACTTTGAAAATCCTATTTTTGATATGAAAAGATCAACAAATGAATTTATTGGTCGTTCTGCTATGGTGACAAAA
TTACTTTGTATCTATACTGAGGGAGATCCTTTCTTTGGTGTAAATATTAATAGTCAGAAAGAATTTTGGAACCACTTTGT
TTCTCAGACAAATCAAGGTGGACCTTATCTGCAAAATCATAAAATAATTGAACTGGTGTCCAAAACTTATCCTGAGTTGG
AGCCATCGAAATTAGGAACTATGCTTTTTGAGTATTCTAAGCTTTTTATGGAAAATAAGGAAGACAATAGTACAATGGAT
TCATCAAACAATTTTAGTCATCAATTAACTCAATCTCTATTAAAGTCTCCAAACCTTATCCTTCGTGGTGCTCCTGGCAC
AGGAAAGACTTATCTTGCTAAAGAAATTGCCAAAGAATTAACGGATGGCAACGAAGACCAAATCGGATTTGTACAATTTC
ACCCATCATATGATTATACGGATTTTGTAGAAGGTTTAAGACCAGTATCAAATGGGGATGGAGCTATTGAGTTTAGGCTA
CAGGACGGTATTTTTAAAGATTTTTGTCAGAAAGCAAAAGAAACCCAATTGATTGGAGGACAAGATAATTTTGATGAGGC
TTGGGATTCTTACTTAGAATATATAAATGTTGCTGAAGAAAAAGAATATATAACAAAAACATCTTACTTATCTGTTAATA
GTAGACAAAATTTATCAGTAAATTATGATAGTGGTGTTCCAGGATGGTCACTACCTAGCAAATATGTTTACGAGTTGTAT
AAAGATAAAAATTATAATAAGCAAGAATACTACAAAAGTGGTGGAAAAACTGTCCTAGAAACATTGAGAAAGAGATTTGG
TTTGAAAGACTATGTTTCCCCAACAGAAATTGATACTGATAAGAAATTCGTTTTCATCATCGATGAAATCAATCGTGGGG
AGATTTCTAAGATTTTTGGCGAACTCTTTTTCTCTATCGACCCTGGCTATCGTGGTGAAAAAGGAAGTGTTTCTACCCAA
TATGCAAATCTACACGAAACTGATGAAAAGTTCTATATCCCCGAAAATGTTTACATCATCGGAACTATGAATGATATTGA
TCGTTCAGTGGATACCTTTGATTTTGCTATGCGTCGTCGTTTTCGTTTTGTTGAAGTTACTGCCGAGGGTCAAGTTGGCA
TGTTGGATAAAGAGCTGAATATCCATGCAGAAGAAGCAAAAATTCGTCTAAGAAACTTGAACGCTGCTATCGAAAATGTT
CAGGAATTAAACAGTCATTATCATATTGGACCAAGTTATTTTCTTAAGTTGAAGGATGTAGATTTTGACTATGAATTACT
CTGGTCTGATTATATTAAGCCTCTCCTAGAAGACTACTTGCGAGGTTCTTATGATGAGGTTGAAACTTTGGAAACTTTGA
AAAAAGCATTTGAACTGACAAATAATGATCAAACTGGTCAACAAGATACTGGTGATAATGATGCGGATAACTGA

Upstream 100 bases:

>100_bases
TTTCTTTTCTTTTTCTATAGGATATGGTATTCTATGTAGAAAATATATGTTAATAAGTAATGCCAATATTTAAACATCAT
TAGTAAAAGGAGTTAGATTG

Downstream 100 bases:

>100_bases
TAATCAGCATAAAATTGCTAAAGAAGACTTTGTCGCAGAATATCCCAAACTAAGTCAAGCTCTTCTTGATAGAACACTGG
ATAACCTTTCTAGAGAGGAT

Product: endonuclease

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMD
SSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQTGQQDTGDNDADN

Sequences:

>Translated_637_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMD
SSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQTGQQDTGDNDADN
>Mature_637_residues
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNL
FLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMD
SSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRL
QDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELY
KDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENV
QELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQTGQQDTGDNDADN

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=174, Percent_Identity=38.5057471264368, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 73863; Mature: 73863

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHEEHHEHHHHHHC
KGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSK
CCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
LKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYTEGDPFFGVNINSQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCHH
EFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYT
CCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
DFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEE
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHH
KEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLE
HHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHH
TLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEH
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELN
CCCCCCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEECCCCC
IHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYL
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
RGSYDEVETLETLKKAFELTNNDQTGQQDTGDNDADN
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEWPLDRILTMSIDEYVIG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHEEHHEHHHHHHC
KGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNKYKDQANNEISELDQRFSK
CCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHH
LKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYTEGDPFFGVNINSQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECCCHH
EFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEYSKLFMENKEDNSTMD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDGNEDQIGFVQFHPSYDYT
CCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
DFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDNFDEAWDSYLEYINVAEE
HHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHH
KEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLE
HHHHHHHHEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHH
TLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQ
HHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEH
YANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELN
CCCCCCCCCCEECCCCEEEEEECHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEECCCCC
IHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYL
CCHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHH
RGSYDEVETLETLKKAFELTNNDQTGQQDTGDNDADN
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]