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The map label for this gene is ask [H]

Identifier: 221230725

GI number: 221230725

Start: 2752604

End: 2753869

Strand: Reverse

Name: ask [H]

Synonym: MLBr_02323

Alternate gene names: 221230725

Gene position: 2753869-2752604 (Counterclockwise)

Preceding gene: 221230729

Following gene: 221230724

Centisome position: 84.27

GC content: 61.3

Gene sequence:

>1266_bases
GTGGCGCTTGTCGTGCAGAAGTATGGTGGATCCTCGGTGGCCGACGCCGATCGGATTCGCCGTGTTGCTGAGCGCATCGT
TCAGACCAAAAAACAGGGCAATGACATCGTTGTGGTGGTTTCCGCAATGGGCGACACCACCGACGACCTGCTAGACCTAG
CGCAGCAGGTATGCCCGGAGCCGCCGGCCCGCGAGTTGGACATGCTGCTGACCGCGGGGGAGCGCATCTCCAATGCGCTG
GTGGCCATGGCTATCGAATCATTCGGTGCCCAGGCACGCTCGTTCACCGGTTCGCAAGCTGGCGTTATCACCACCGGCAC
CCACGGCAACGCCAAAATCATCGATGTCACCCCGACCCGGCTGCAGTTCGCGCTCGACGAGGGGCGAATTGTGTTGGTCG
CCGGATTCCAGGGCGTCAGCCAAGACACCCGCGATATCACCACGCTGGGTCGCGGCGGCTCGGACACCACCGCAATTGCC
ATGGCCGCGGCATTAGGCGCCGATGTATGCGAGATCTACACCGACGTGGACGGTATCTTCAGCGCCGACCCACGGGTCGT
GCACAACGCCCGCAAGCTCGACACCGTGACCTTCGAGGAGATGCTCGAGATGGCGGCTTGCGGCGCTAAGGTGCTTATGT
TGCGCTGCGTGGAATACGCACGCCGCCACAACATTCCGGTGCACGTCCGATCGTCGTATGCGGACAAACCAGGCACCGTC
ATCGTTGGATCGATCAAGGACGTACCTATGGAAGACCCCATCCTGACCGGAGTTGCGCACGATCGCAGCGAGGCCAAGGT
GACCATCGTCGGGATACCCGACATCCCGGGTTACGCGGCCAAAGTGTTTCGCGCTGTCGCCGACGCTGATGTGAACATTG
ATATGGTGTTGCAAAACGTATCCAAGGTCGAAGACGGCAAGACCGATATCACTTTCACCTGCTCCCGCGATAGCGGGCCC
ATCGCGGTGGCCAAGCTGGGCTCGCTCAGAGACGAGATCGGCTTCACCCAGCTGCTCTATGACGATCACATCGGCAAGGT
ATCGTTGATCGGAGCCGGCATGCGCAGTCACCCTGGGGTCACGGCGACCTTCTGCGAAGCGCTGGCTGCTGTTGGGGTCA
ACATCGAGCTCATCTCCACCTCGGAAATCCGGATATCGGTGCTGTGCCGCGATACCGAACTAGACAAGGCCGTCTTTGCG
TTGCATGAGGCGTTTGGACTCGGTGGCGAGGACGAGGTGACGGTGTACGCGGGGACGGGGAGGTAA

Upstream 100 bases:

>100_bases
TGCATCGTCGCAGTGCCAGCCCCACGTATTCTGTTTTGGACCTAACTAGCCAGGTATATAACCAGAAAGGCAGGTATATA
ACCAGAAAGGCAGTGCAACA

Downstream 100 bases:

>100_bases
TGGCTACCAGAGGTTTGGCAATCGGGGTAGTGGGTGCCACTGGCCAAGTCGGCCACGTGATGCGCACACTGCTCGAACAG
CGCGATTTCCGGGCCGATTC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MALVVQKYGGSSVADADRIRRVAERIVQTKKQGNDIVVVVSAMGDTTDDLLDLAQQVCPEPPARELDMLLTAGERISNAL
VAMAIESFGAQARSFTGSQAGVITTGTHGNAKIIDVTPTRLQFALDEGRIVLVAGFQGVSQDTRDITTLGRGGSDTTAIA
MAAALGADVCEIYTDVDGIFSADPRVVHNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYADKPGTV
IVGSIKDVPMEDPILTGVAHDRSEAKVTIVGIPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDSGP
IAVAKLGSLRDEIGFTQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVFA
LHEAFGLGGEDEVTVYAGTGR

Sequences:

>Translated_421_residues
MALVVQKYGGSSVADADRIRRVAERIVQTKKQGNDIVVVVSAMGDTTDDLLDLAQQVCPEPPARELDMLLTAGERISNAL
VAMAIESFGAQARSFTGSQAGVITTGTHGNAKIIDVTPTRLQFALDEGRIVLVAGFQGVSQDTRDITTLGRGGSDTTAIA
MAAALGADVCEIYTDVDGIFSADPRVVHNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYADKPGTV
IVGSIKDVPMEDPILTGVAHDRSEAKVTIVGIPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDSGP
IAVAKLGSLRDEIGFTQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVFA
LHEAFGLGGEDEVTVYAGTGR
>Mature_420_residues
ALVVQKYGGSSVADADRIRRVAERIVQTKKQGNDIVVVVSAMGDTTDDLLDLAQQVCPEPPARELDMLLTAGERISNALV
AMAIESFGAQARSFTGSQAGVITTGTHGNAKIIDVTPTRLQFALDEGRIVLVAGFQGVSQDTRDITTLGRGGSDTTAIAM
AAALGADVCEIYTDVDGIFSADPRVVHNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYADKPGTVI
VGSIKDVPMEDPILTGVAHDRSEAKVTIVGIPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDSGPI
AVAKLGSLRDEIGFTQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVFAL
HEAFGLGGEDEVTVYAGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=26.3498920086393, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790455, Length=174, Percent_Identity=38.5057471264368, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320893, Length=405, Percent_Identity=27.6543209876543, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44664; Mature: 44533

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGSSVADADRIRRVAERIVQTKKQGNDIVVVVSAMGDTTDDLLDLAQQVCPE
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPARELDMLLTAGERISNALVAMAIESFGAQARSFTGSQAGVITTGTHGNAKIIDVTPTR
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCCCCEEEEEECCCE
LQFALDEGRIVLVAGFQGVSQDTRDITTLGRGGSDTTAIAMAAALGADVCEIYTDVDGIF
EEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCC
SADPRVVHNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYADKPGTV
CCCCHHHHCHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
IVGSIKDVPMEDPILTGVAHDRSEAKVTIVGIPDIPGYAAKVFRAVADADVNIDMVLQNV
EEECCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCSRDSGPIAVAKLGSLRDEIGFTQLLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCHHHEEHHCCCCEEEEEECCCCCCCCC
TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVFALHEAFGLGGEDEVTVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C
>Mature Secondary Structure 
ALVVQKYGGSSVADADRIRRVAERIVQTKKQGNDIVVVVSAMGDTTDDLLDLAQQVCPE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPARELDMLLTAGERISNALVAMAIESFGAQARSFTGSQAGVITTGTHGNAKIIDVTPTR
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEECCCCCEEEEEECCCE
LQFALDEGRIVLVAGFQGVSQDTRDITTLGRGGSDTTAIAMAAALGADVCEIYTDVDGIF
EEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCC
SADPRVVHNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYADKPGTV
CCCCHHHHCHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
IVGSIKDVPMEDPILTGVAHDRSEAKVTIVGIPDIPGYAAKVFRAVADADVNIDMVLQNV
EEECCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCSRDSGPIAVAKLGSLRDEIGFTQLLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCHHHEEHHCCCCEEEEEECCCCCCCCC
TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVFALHEAFGLGGEDEVTVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972; 7910936 [H]