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The map label for this gene is phoS3 [H]

Identifier: 221230649

GI number: 221230649

Start: 2606756

End: 2608045

Strand: Reverse

Name: phoS3 [H]

Synonym: MLBr_02192

Alternate gene names: 221230649

Gene position: 2608045-2606756 (Counterclockwise)

Preceding gene: 221230650

Following gene: 221230648

Centisome position: 79.8

GC content: 57.29

Gene sequence:

>1290_bases
ATGACTGAGCGAAAGCCCAGTTTCATGCAGTATTCACCGGTGTTGTTCACCCCAATAGCGATGTTTCTGGGTTCCTGCGG
TGCATACTTTCCGGGTGACCAGGCCACTTTGCGCTCGGCCAGCGGTGCGGGTGTGCGTCGAAAGTGCGCTGAGAAAGCGA
GATCGGTGAACCACGACAAGGTATGCAGGGCGTTTGCGATGGTGTTGACGACGGCGGTCACCGCCGGGGTGTTATCCGCG
TGCGGTAGTGACGTCAATCGCGGCCATACTGCGACAACATCCATCTCAGGGAAATCGGCTGACACCGCGGCTTGCGGCGG
CAAAAGAACAATGACGGCTGAAGGGTCGACCGCTCAACAGAACGCGATCGCATTGTTTAATCAGATTTGGGGAAAGTTGT
GCCCTGACAAGACCGTGTCATACAACCCGACTGGGTCGGGTGCTGGTCGTGAGCAATTCATTGCCGGCCGTATCGACTTC
GCTGGCTCGGACTCACCGCTGCTTGCGGATCAACTCGGCCCGGCCGCCAAACGCTGTAAAGGCAACCCGGCCTGGGATCT
GCCGCTGGTGTTCGGTCCGGTTGCGTTGGTTTACAACCTGCCAGGCGTTCAAATGTTGACCGTCAATAGTGACGTGTTGG
CCAAAATCTTCAGTGGCGTGATTACGACATGGAACGACGGGATACTGGCGGCTCTCAATCCAGGTGTTGTCCTGCCCAAC
CTCAGGATCATGCCGATCTATCGGGCGGATGCATCGGGAACCACCGACAACTTGCAGAAGTATTTGACGACCGATGCACC
ACAGAGCTGGGCCAAAGGGGTCGGTGCCGAGTTTCAGGGCGGTGTTGGCGAAGGGGTCCAGAAGTCGGCTGGTGTAATCC
AGGCGGTGCAGACTACTCCAGGCGCCTTTGGTTATGTTGAGAAGAGCTTCGCTGATCGGGCTGGATTGCCGTTCGCCCAG
ATTGACACCGGCAGTGGCGGCGTACCGCTTACCGACGACACGGCACGCAATGGCATAAACGCAGCCAACTTCGTGTCCGT
CGGCAACGACCTAGTACTGGACCTGAACGCGATGTACAGCACCGAACAGTCTAATGCTTACCCGTTGGTCTTGGCCACAT
ATGAAATCGTCTGCTCGAAAGGCTACGATCCGGACACGTTTGCGGCGATCAAGTCATTCCTCACCGTGGCAGCCAATGAC
GGTCAGGCCGGTCTTGCAGCGGTCGGCTATGTCCCGCTGCCCGATACAATTAAAAAACGACTGGTCAGCGCGATCAATGC
AATGCAGTAG

Upstream 100 bases:

>100_bases
CTCTATGTGGAATCGGACAACGTCGCCGCGGCGCGGACCTACGAGCGGCTAGGCTTTACCACGTACAGTGTTGACACTGC
TTACGCACTAGCCCGGATCG

Downstream 100 bases:

>100_bases
CAAATTGTCTCTGTGCGACATCTTTTCTCAAGCGGGCAACAGGCGGTTTAAAAGTGACGGCAGAAATAACAGGGATGGGA
TTACGGTTACAACGCCAACC

Product: phosphate-binding protein 3 precursor

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 3; PstS-3; Antigen Ag88 [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTERKPSFMQYSPVLFTPIAMFLGSCGAYFPGDQATLRSASGAGVRRKCAEKARSVNHDKVCRAFAMVLTTAVTAGVLSA
CGSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQNAIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDF
AGSDSPLLADQLGPAAKRCKGNPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPN
LRIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGAEFQGGVGEGVQKSAGVIQAVQTTPGAFGYVEKSFADRAGLPFAQ
IDTGSGGVPLTDDTARNGINAANFVSVGNDLVLDLNAMYSTEQSNAYPLVLATYEIVCSKGYDPDTFAAIKSFLTVAAND
GQAGLAAVGYVPLPDTIKKRLVSAINAMQ

Sequences:

>Translated_429_residues
MTERKPSFMQYSPVLFTPIAMFLGSCGAYFPGDQATLRSASGAGVRRKCAEKARSVNHDKVCRAFAMVLTTAVTAGVLSA
CGSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQNAIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDF
AGSDSPLLADQLGPAAKRCKGNPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPN
LRIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGAEFQGGVGEGVQKSAGVIQAVQTTPGAFGYVEKSFADRAGLPFAQ
IDTGSGGVPLTDDTARNGINAANFVSVGNDLVLDLNAMYSTEQSNAYPLVLATYEIVCSKGYDPDTFAAIKSFLTVAAND
GQAGLAAVGYVPLPDTIKKRLVSAINAMQ
>Mature_428_residues
TERKPSFMQYSPVLFTPIAMFLGSCGAYFPGDQATLRSASGAGVRRKCAEKARSVNHDKVCRAFAMVLTTAVTAGVLSAC
GSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQNAIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDFA
GSDSPLLADQLGPAAKRCKGNPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPNL
RIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGAEFQGGVGEGVQKSAGVIQAVQTTPGAFGYVEKSFADRAGLPFAQI
DTGSGGVPLTDDTARNGINAANFVSVGNDLVLDLNAMYSTEQSNAYPLVLATYEIVCSKGYDPDTFAAIKSFLTVAANDG
QAGLAAVGYVPLPDTIKKRLVSAINAMQ

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=290, Percent_Identity=28.9655172413793, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44705; Mature: 44574

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKPSFMQYSPVLFTPIAMFLGSCGAYFPGDQATLRSASGAGVRRKCAEKARSVNHDK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHH
VCRAFAMVLTTAVTAGVLSACGSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCCEEEECCCCCHHH
NAIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDFAGSDSPLLADQLGPAAKRCK
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHEEEEEEECCCCCCCCHHHHCCHHHHHCC
GNPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPN
CCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCEECCC
LRIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGAEFQGGVGEGVQKSAGVIQAVQTTP
CEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCEEEHHCCC
GAFGYVEKSFADRAGLPFAQIDTGSGGVPLTDDTARNGINAANFVSVGNDLVLDLNAMYS
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHEECCCCEEEEEHHHHC
TEQSNAYPLVLATYEIVCSKGYDPDTFAAIKSFLTVAANDGQAGLAAVGYVPLPDTIKKR
CCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHH
LVSAINAMQ
HHHHHHHCC
>Mature Secondary Structure 
TERKPSFMQYSPVLFTPIAMFLGSCGAYFPGDQATLRSASGAGVRRKCAEKARSVNHDK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCHHH
VCRAFAMVLTTAVTAGVLSACGSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCCEEEECCCCCHHH
NAIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDFAGSDSPLLADQLGPAAKRCK
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHEEEEEEECCCCCCCCHHHHCCHHHHHCC
GNPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPN
CCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCEECCC
LRIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGAEFQGGVGEGVQKSAGVIQAVQTTP
CEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCEEEHHCCC
GAFGYVEKSFADRAGLPFAQIDTGSGGVPLTDDTARNGINAANFVSVGNDLVLDLNAMYS
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHEECCCCEEEEEHHHHC
TEQSNAYPLVLATYEIVCSKGYDPDTFAAIKSFLTVAANDGQAGLAAVGYVPLPDTIKKR
CCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHEECCCCCCCEEEEECCCCCHHHHHH
LVSAINAMQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]