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The map label for this gene is prrB [H]

Identifier: 221230617

GI number: 221230617

Start: 2524794

End: 2526134

Strand: Direct

Name: prrB [H]

Synonym: MLBr_02124

Alternate gene names: 221230617

Gene position: 2524794-2526134 (Clockwise)

Preceding gene: 221230616

Following gene: 221230623

Centisome position: 77.26

GC content: 63.39

Gene sequence:

>1341_bases
ATGAATATCCTGTCGCGAATCTTCGCCCGTACGCCGTCGTTGCGAACCCGGGTGGTGGTCGCTACGGCCATCGGTGCAGC
GATTCCGGTTCTCATCGTCGGCACAGTCGTCTGGGTCGGGATCACCAACGACCGCAAAGAGCGGCTGGACCGCAAACTGG
ATGAGGCAGCCGGTTTCGCGATCCCGTTCGTGCCGCGCGGCCTTGACGAAATTCCGCGCTCGCCTAACGACCAGGACGCC
ATCATCACCGTGCGCCGCGGCAACTTGGTCAAGTCGAATTTCGACATCACGCTGCCCAAACTGACAAACGACTACGCTGA
TACCTACCTCCGCGGGGTACGCTACCGAGTACGGACGGTGGAGATCCCAGCACCGGAGCCGACATCAATTGCTGTCGGCG
CGACGTACGACGCCACCGTCGCCGAGACCAACAACTTGCACCGCCGAGTGCTACTGATCTGTGGATTCGCCATCGCTGCG
GCGGCCGTTTTCGCCTGGCTGCTGGCCGCATTCGCAGTGCGCCCGTTCAAACAGCTTGCTCAGCAGACCCGATCAGTCGA
TGCCGGCGGTGAGGCACCACGGGTGGAGGTGCACGGCGCCACCGAAGCCGTTGAGATCGCCGAGGCGATGAGAGGAATGC
TGCAGCGTATCTGGAATGAACAGAACCGGACCAAGGAGGCGCTGGCCTCGGCCCGCGATTTCGCTGCAGTGTCCTCCCAT
GAACTACGCACACCATTGACTGCGATGCGCACTAATCTCGAAGTGCTTGCCACCCTGGACTTGGCCGACGACCAACGCAA
AGAAGTCCTGGGCGACGTGATCCGCACCCAGTCGCGGATAGAGGCCACCCTCAGCGCGCTGGAGCGGTTGGCCCAGGGAG
AACTGTCGACGTCGGACGATCACGTGCCAGTCGACATCACTGAGTTGCTCGACCGTGCCGCCCACGACGCGACTCGGAGC
TACCCTGAGCTCAAAGTCTCGCTGGTGCCGTCGCCGACCTGCATCATTGTGGGGTTGCCAGCCGGGTTACGACTTGCCGT
CGACAACGCGGTCGCCAATGCCGTGAAACATGGCGGCGCCACCCGAGTCCAGCTGTCCGCGGTCAGTTCGCGGGCCGGGG
TAGAAATTGCCGTCGACGACAACGGCAGTGGAGTGCCCGAAGATGAGCGCCAAGTGGTGTTTGAACGGTTTTCTCGAGGA
TCGACGGCCTCCCACTCGGGATCGGGTCTGGGGCTGGCGCTGGTGGCCCAGCAGGCCCAGCTACACGGCGGGACGGCTTC
ATTGGAAACTAGCCCGCTGGGCGGCGCGCGGCTGCTGCTGCGTATATCCGCGCCCAGCTAA

Upstream 100 bases:

>100_bases
TGCGCCGCAAGTTGGAGGCCAACAGCGGGCCCAGGCTACTGCACACCGTTCGAGGAGTTGGGTTCGTACTGCGCATGCAG
TAACCGGATCGAAGCAGGCC

Downstream 100 bases:

>100_bases
CCGAAAGAACCTCGCTGAATGCCTTTATTTCCGTGAACTCTTGCGCCACGGCGTGAAGTAGGCCCTTTTCGCGTATTCCG
TATCCTCGAACCCTATCTGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRKLDEAAGFAIPFVPRGLDEIPRSPNDQDA
IITVRRGNLVKSNFDITLPKLTNDYADTYLRGVRYRVRTVEIPAPEPTSIAVGATYDATVAETNNLHRRVLLICGFAIAA
AAVFAWLLAAFAVRPFKQLAQQTRSVDAGGEAPRVEVHGATEAVEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLATLDLADDQRKEVLGDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITELLDRAAHDATRS
YPELKVSLVPSPTCIIVGLPAGLRLAVDNAVANAVKHGGATRVQLSAVSSRAGVEIAVDDNGSGVPEDERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLETSPLGGARLLLRISAPS

Sequences:

>Translated_446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRKLDEAAGFAIPFVPRGLDEIPRSPNDQDA
IITVRRGNLVKSNFDITLPKLTNDYADTYLRGVRYRVRTVEIPAPEPTSIAVGATYDATVAETNNLHRRVLLICGFAIAA
AAVFAWLLAAFAVRPFKQLAQQTRSVDAGGEAPRVEVHGATEAVEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLATLDLADDQRKEVLGDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITELLDRAAHDATRS
YPELKVSLVPSPTCIIVGLPAGLRLAVDNAVANAVKHGGATRVQLSAVSSRAGVEIAVDDNGSGVPEDERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLETSPLGGARLLLRISAPS
>Mature_446_residues
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRKLDEAAGFAIPFVPRGLDEIPRSPNDQDA
IITVRRGNLVKSNFDITLPKLTNDYADTYLRGVRYRVRTVEIPAPEPTSIAVGATYDATVAETNNLHRRVLLICGFAIAA
AAVFAWLLAAFAVRPFKQLAQQTRSVDAGGEAPRVEVHGATEAVEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
ELRTPLTAMRTNLEVLATLDLADDQRKEVLGDVIRTQSRIEATLSALERLAQGELSTSDDHVPVDITELLDRAAHDATRS
YPELKVSLVPSPTCIIVGLPAGLRLAVDNAVANAVKHGGATRVQLSAVSSRAGVEIAVDDNGSGVPEDERQVVFERFSRG
STASHSGSGLGLALVAQQAQLHGGTASLETSPLGGARLLLRISAPS

Specific function: Member of the two-component regulatory system prrB/prrA. Involved specifically in early intracellular multiplication of Mycobacterium. Probably phosphorylates prrA [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=207, Percent_Identity=31.4009661835749, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789808, Length=298, Percent_Identity=28.5234899328859, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=31.1203319502075, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789403, Length=470, Percent_Identity=25.7446808510638, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790861, Length=307, Percent_Identity=28.0130293159609, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=28.7272727272727, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=25.2032520325203, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=441, Percent_Identity=28.1179138321995, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788279, Length=287, Percent_Identity=25.0871080139373, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=213, Percent_Identity=30.0469483568075, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787374, Length=276, Percent_Identity=26.8115942028986, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=341, Percent_Identity=22.5806451612903, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790551, Length=286, Percent_Identity=25.1748251748252, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47797; Mature: 47797

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRKLDEAAGFA
CCHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCC
IPFVPRGLDEIPRSPNDQDAIITVRRGNLVKSNFDITLPKLTNDYADTYLRGVRYRVRTV
CCCCCCCHHHCCCCCCCCCEEEEEECCCEEECCCCEECCCCCCHHHHHHHHHCEEEEEEE
EIPAPEPTSIAVGATYDATVAETNNLHRRVLLICGFAIAAAAVFAWLLAAFAVRPFKQLA
ECCCCCCCEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQTRSVDAGGEAPRVEVHGATEAVEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
HHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
ELRTPLTAMRTNLEVLATLDLADDQRKEVLGDVIRTQSRIEATLSALERLAQGELSTSDD
HHHCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HVPVDITELLDRAAHDATRSYPELKVSLVPSPTCIIVGLPAGLRLAVDNAVANAVKHGGA
CCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCEEEHHHHHHHHHHCCCC
TRVQLSAVSSRAGVEIAVDDNGSGVPEDERQVVFERFSRGSTASHSGSGLGLALVAQQAQ
CEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHGGTASLETSPLGGARLLLRISAPS
HCCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MNILSRIFARTPSLRTRVVVATAIGAAIPVLIVGTVVWVGITNDRKERLDRKLDEAAGFA
CCHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCC
IPFVPRGLDEIPRSPNDQDAIITVRRGNLVKSNFDITLPKLTNDYADTYLRGVRYRVRTV
CCCCCCCHHHCCCCCCCCCEEEEEECCCEEECCCCEECCCCCCHHHHHHHHHCEEEEEEE
EIPAPEPTSIAVGATYDATVAETNNLHRRVLLICGFAIAAAAVFAWLLAAFAVRPFKQLA
ECCCCCCCEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQTRSVDAGGEAPRVEVHGATEAVEIAEAMRGMLQRIWNEQNRTKEALASARDFAAVSSH
HHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCH
ELRTPLTAMRTNLEVLATLDLADDQRKEVLGDVIRTQSRIEATLSALERLAQGELSTSDD
HHHCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HVPVDITELLDRAAHDATRSYPELKVSLVPSPTCIIVGLPAGLRLAVDNAVANAVKHGGA
CCCEEHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCEEEHHHHHHHHHHCCCC
TRVQLSAVSSRAGVEIAVDDNGSGVPEDERQVVFERFSRGSTASHSGSGLGLALVAQQAQ
CEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LHGGTASLETSPLGGARLLLRISAPS
HCCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]