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The map label for this gene is gcvH

Identifier: 221230600

GI number: 221230600

Start: 2469393

End: 2469791

Strand: Reverse

Name: gcvH

Synonym: MLBr_02077

Alternate gene names: 221230600

Gene position: 2469791-2469393 (Counterclockwise)

Preceding gene: 221230601

Following gene: 221230599

Centisome position: 75.57

GC content: 58.15

Gene sequence:

>399_bases
GTGAGTGATATCCCGTCCGATCTGCATTACACTGCTGAGCATGAGTGGATTCGGCGCAGTCGCGAAGACACCGTCCGGGT
CGGGCTCACCGACTTCGCGCAGTCGACACTGGGTGATGTGGTTTTCGTCCAGCTGCCAGAAGTTGGCGCTGAGTTAGCGG
CGGGCAAATCCTTCGGCGAAGTGGAATCGACGAAATCGGTGTCGGACTTGTATGCACCGGTGTCGGGTACAGTGTCGGCG
GTAAACACCGATCTGGAAGGCAGTCCCCAACTAGTGAACTCTGACCCATACGGCGCTGGCTGGCTACTGGATGTTCACGT
CTCCGACGTCGGGGCTTTGGAGTCAGCTATCGCAACGCTGCTTGACGCCGAGACTTATCGCGGCACGCTAACCAAATGA

Upstream 100 bases:

>100_bases
GCAACAAGTCGATCGAGTGGACGTAACCGCCTTGCGGCCACCAAAATCGCACCAATACGCTCAGCCCGTCAAGTAGATCA
CCGACCAGAATAGGATTACC

Downstream 100 bases:

>100_bases
TGATTGCTAGGGTGCTTGGCAATCCCACGTGACGATGATGCGGACGCCGGGCTGCTCGAGGAGGAGCTGGGCAATGAGGG
TCCGATCCTGTGGTGGTTGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSDIPSDLHYTAEHEWIRRSREDTVRVGLTDFAQSTLGDVVFVQLPEVGAELAAGKSFGEVESTKSVSDLYAPVSGTVSA
VNTDLEGSPQLVNSDPYGAGWLLDVHVSDVGALESAIATLLDAETYRGTLTK

Sequences:

>Translated_132_residues
MSDIPSDLHYTAEHEWIRRSREDTVRVGLTDFAQSTLGDVVFVQLPEVGAELAAGKSFGEVESTKSVSDLYAPVSGTVSA
VNTDLEGSPQLVNSDPYGAGWLLDVHVSDVGALESAIATLLDAETYRGTLTK
>Mature_131_residues
SDIPSDLHYTAEHEWIRRSREDTVRVGLTDFAQSTLGDVVFVQLPEVGAELAAGKSFGEVESTKSVSDLYAPVSGTVSAV
NTDLEGSPQLVNSDPYGAGWLLDVHVSDVGALESAIATLLDAETYRGTLTK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=40.3361344537815, Blast_Score=92, Evalue=1e-19,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=39.4957983193277, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789271, Length=130, Percent_Identity=50, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=46.6101694915254, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=45.2991452991453, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=42.8571428571429, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17865652, Length=128, Percent_Identity=39.0625, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYCLB (B8ZSP0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504016.1
- ProteinModelPortal:   B8ZSP0
- SMR:   B8ZSP0
- EnsemblBacteria:   EBMYCT00000086899
- GeneID:   7325145
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015839
- HOGENOM:   HBG693789
- OMA:   NDDPYDG
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14071; Mature: 13939

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIPSDLHYTAEHEWIRRSREDTVRVGLTDFAQSTLGDVVFVQLPEVGAELAAGKSFGE
CCCCCCCCEECHHHHHHHCCCCCEEEECHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCC
VESTKSVSDLYAPVSGTVSAVNTDLEGSPQLVNSDPYGAGWLLDVHVSDVGALESAIATL
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHHHHH
LDAETYRGTLTK
HHHHHCCCCCCC
>Mature Secondary Structure 
SDIPSDLHYTAEHEWIRRSREDTVRVGLTDFAQSTLGDVVFVQLPEVGAELAAGKSFGE
CCCCCCCEECHHHHHHHCCCCCEEEECHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCC
VESTKSVSDLYAPVSGTVSAVNTDLEGSPQLVNSDPYGAGWLLDVHVSDVGALESAIATL
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCCCCCCCEEEEEECCCHHHHHHHHHHH
LDAETYRGTLTK
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA