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The map label for this gene is ispH [H]

Identifier: 221230520

GI number: 221230520

Start: 2324488

End: 2325495

Strand: Reverse

Name: ispH [H]

Synonym: MLBr_01938

Alternate gene names: 221230520

Gene position: 2325495-2324488 (Counterclockwise)

Preceding gene: 221230525

Following gene: 221230518

Centisome position: 71.16

GC content: 61.31

Gene sequence:

>1008_bases
GTGAAGGGCATGCCGCCAACTGTGAACATGGGAATTCCCGGTGCTTCGAGGTCAATAGTTGAAAACCTAAAGCGCAAACG
GGTCCTGTTGGCCGAACCGCGTGGCTACTGCGCAGGGGTGGATCGCGCCGTCGAAACGGTCGAGCGGTCGCTGCGGAAGT
ATGGCCCCCCCGTCTATGTGCGCCATGAGATCGTACATAACCGGCACGTGGTCGAGACCTTGGAAAGGGCCGGTGCGGTT
TTTGTCGAGGAGACCGACTACGTTCCCGAGGGCGCCATCGTGATTTTCTCCGCTCACGGGGTCGCACCAACGGTGTATGC
GGCCGCGGCCGAGCGGAATCTGCGGACCATTGACGCCACTTGTCCGCTTGTCACCAAGGTGCACAACGAGGTCAAGCGGT
TCGCCCGTAACGATTATGACATCCTGCTGATCGGGCATGAGGGCCACGAGGAAGTCATCGCAACCGCCGGAGAGGCGCCC
ACGCATGTGCAACTTGTCGACGGGCTGGCCGCTGTGCAGCAGGTAGTGGTTCGCGACGAGAACAAGGTGGTCTGGTTGTC
ACAGACCACGCTGTCGGTGGACGAGACCATGCGGATCGTCGAGCGGTTGCGGCAGCGCTTCCCGAAGTTGCAAGACCCAC
CTAGCGACGACATTTGCTATGCTACCCAGAACCGGCAGGTCGCGGTCAAGGCGATGGCTCCCGAGTGCGAGCTGGTCATC
GTCGTCGGGTCTCGAAATTCCTCGAATTCGGTGCGACTGGTTGAGGTGGCAATGGGTAGCGGCGCTGGTGCCGCGTACCT
GGTTGACTGGGCCAAGGATATCGACCCGGCGTGGCTGGCCGGGGTCACGACAGTGGGTGTCACCTCGGGGGCATCTGTTC
CCGATATACTAGTGCAAGGCGTACTGAAGTGGTTGGCTGAACGCGGCTACGATGTGGTTCAGTCAGTGACGACTGCCAAC
GAGGCACTGGTGTTTGCGCTGCCCCGGGAGATCCGGTCTGCGCACTAA

Upstream 100 bases:

>100_bases
ATCGTAAGTGTGCCGGAGCCGGGTGCAGCGGATCGCTACTCAATCAAACCCAGGCGCCGTCTAGGTAGGCAGATAAAGTA
CTCTATCGACGGGAAGTAAC

Downstream 100 bases:

>100_bases
GCGACCGGACTCAAACGTCGAATTCCCAGGATTCGGTCACGGGTCGGCGGGGTGTCTGGGATCGTTGTCGGTCGACACGA
GGATCGTCTCGGGCGGTTGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKGMPPTVNMGIPGASRSIVENLKRKRVLLAEPRGYCAGVDRAVETVERSLRKYGPPVYVRHEIVHNRHVVETLERAGAV
FVEETDYVPEGAIVIFSAHGVAPTVYAAAAERNLRTIDATCPLVTKVHNEVKRFARNDYDILLIGHEGHEEVIATAGEAP
THVQLVDGLAAVQQVVVRDENKVVWLSQTTLSVDETMRIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVAMGSGAGAAYLVDWAKDIDPAWLAGVTTVGVTSGASVPDILVQGVLKWLAERGYDVVQSVTTAN
EALVFALPREIRSAH

Sequences:

>Translated_335_residues
MKGMPPTVNMGIPGASRSIVENLKRKRVLLAEPRGYCAGVDRAVETVERSLRKYGPPVYVRHEIVHNRHVVETLERAGAV
FVEETDYVPEGAIVIFSAHGVAPTVYAAAAERNLRTIDATCPLVTKVHNEVKRFARNDYDILLIGHEGHEEVIATAGEAP
THVQLVDGLAAVQQVVVRDENKVVWLSQTTLSVDETMRIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVAMGSGAGAAYLVDWAKDIDPAWLAGVTTVGVTSGASVPDILVQGVLKWLAERGYDVVQSVTTAN
EALVFALPREIRSAH
>Mature_335_residues
MKGMPPTVNMGIPGASRSIVENLKRKRVLLAEPRGYCAGVDRAVETVERSLRKYGPPVYVRHEIVHNRHVVETLERAGAV
FVEETDYVPEGAIVIFSAHGVAPTVYAAAAERNLRTIDATCPLVTKVHNEVKRFARNDYDILLIGHEGHEEVIATAGEAP
THVQLVDGLAAVQQVVVRDENKVVWLSQTTLSVDETMRIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
VVGSRNSSNSVRLVEVAMGSGAGAAYLVDWAKDIDPAWLAGVTTVGVTSGASVPDILVQGVLKWLAERGYDVVQSVTTAN
EALVFALPREIRSAH

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=51.1326860841424, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 36542; Mature: 36542

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMPPTVNMGIPGASRSIVENLKRKRVLLAEPRGYCAGVDRAVETVERSLRKYGPPVYV
CCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RHEIVHNRHVVETLERAGAVFVEETDYVPEGAIVIFSAHGVAPTVYAAAAERNLRTIDAT
EHHHHHCHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECC
CPLVTKVHNEVKRFARNDYDILLIGHEGHEEVIATAGEAPTHVQLVDGLAAVQQVVVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
NKVVWLSQTTLSVDETMRIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEE
VVGSRNSSNSVRLVEVAMGSGAGAAYLVDWAKDIDPAWLAGVTTVGVTSGASVPDILVQG
EEECCCCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHEECCCCCCHHHHHHHH
VLKWLAERGYDVVQSVTTANEALVFALPREIRSAH
HHHHHHHCCHHHHHHHHHCCCEEEEEECHHHHCCC
>Mature Secondary Structure
MKGMPPTVNMGIPGASRSIVENLKRKRVLLAEPRGYCAGVDRAVETVERSLRKYGPPVYV
CCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEE
RHEIVHNRHVVETLERAGAVFVEETDYVPEGAIVIFSAHGVAPTVYAAAAERNLRTIDAT
EHHHHHCHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECC
CPLVTKVHNEVKRFARNDYDILLIGHEGHEEVIATAGEAPTHVQLVDGLAAVQQVVVRDE
CHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCC
NKVVWLSQTTLSVDETMRIVERLRQRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVI
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEE
VVGSRNSSNSVRLVEVAMGSGAGAAYLVDWAKDIDPAWLAGVTTVGVTSGASVPDILVQG
EEECCCCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHEECCCCCCHHHHHHHH
VLKWLAERGYDVVQSVTTANEALVFALPREIRSAH
HHHHHHHCCHHHHHHHHHCCCEEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]