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The map label for this gene is def

Identifier: 221230516

GI number: 221230516

Start: 2312540

End: 2313133

Strand: Direct

Name: def

Synonym: MLBr_01929

Alternate gene names: 221230516

Gene position: 2312540-2313133 (Clockwise)

Preceding gene: 221230511

Following gene: 221230517

Centisome position: 70.76

GC content: 60.27

Gene sequence:

>594_bases
ATGGCAATCGCACCCATTCGCATTGTGGGAGATCCAGTCTTACACACACCGACGGCACCGGTGCAAGTCGCCGCTGACGG
TTCGCTACCAGCGAACCTTAACGGATTGATCTCCACAATGTATGACACGATGGACGCCGCCCACGGGGTCGGCCTGGCCG
CTAACCAGATCGGGTACGGACTACGGGTGTTCGTCTACGACTGCGCCGAGGACTGTAGACAGACCGCCCGCCGGCGCGGT
GTGGTCATCAACCCGATCTTGGAAACTTCCGAGATACCCGAAACAATGCCCGACCCGGATACCGACAACGAAGGTTGCTT
ATCGGTTCCGGGAGAGTCATTTCCTATAGGGCGTGCACAGTGGGCACGTGTTACTGGCCTCGACGCCGATGGCAACCCGG
TTACTACCGAAGGCACCGGCCTGTTTGCACGGATGCTGCAACACGAAACCGGGCACCTGGACGGTTTTCTGTACCTGGAC
TATCTCATTGGCCGACACGCTCGCAGCGCCAAGCGAGCCATCAAGTCACGCCACTGGGGCGTTCCCGGACTATCGTGGAT
GCCGGGCGAGGTGCCCGACCCATTCGGTCCCTAA

Upstream 100 bases:

>100_bases
CAGACCCGCGAGTCGGCCAACTTGCTTGGAATGTTCGAACAGATCGTCCGCCACCGCCGCTATGCGATTGCCATGGTCGG
CGGGCTTAAGCTAGCCGACC

Downstream 100 bases:

>100_bases
TGGTCTCGTGACCGAACCCCGAAACCAGGGTAACGGGCCCGGTACCGTCCGTCCTGCCGGTGCGGTCCCATCGCTGACCG
GTGCCGTAAGACACTTCCTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MAIAPIRIVGDPVLHTPTAPVQVAADGSLPANLNGLISTMYDTMDAAHGVGLAANQIGYGLRVFVYDCAEDCRQTARRRG
VVINPILETSEIPETMPDPDTDNEGCLSVPGESFPIGRAQWARVTGLDADGNPVTTEGTGLFARMLQHETGHLDGFLYLD
YLIGRHARSAKRAIKSRHWGVPGLSWMPGEVPDPFGP

Sequences:

>Translated_197_residues
MAIAPIRIVGDPVLHTPTAPVQVAADGSLPANLNGLISTMYDTMDAAHGVGLAANQIGYGLRVFVYDCAEDCRQTARRRG
VVINPILETSEIPETMPDPDTDNEGCLSVPGESFPIGRAQWARVTGLDADGNPVTTEGTGLFARMLQHETGHLDGFLYLD
YLIGRHARSAKRAIKSRHWGVPGLSWMPGEVPDPFGP
>Mature_196_residues
AIAPIRIVGDPVLHTPTAPVQVAADGSLPANLNGLISTMYDTMDAAHGVGLAANQIGYGLRVFVYDCAEDCRQTARRRGV
VINPILETSEIPETMPDPDTDNEGCLSVPGESFPIGRAQWARVTGLDADGNPVTTEGTGLFARMLQHETGHLDGFLYLDY
LIGRHARSAKRAIKSRHWGVPGLSWMPGEVPDPFGP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=169, Percent_Identity=33.7278106508876, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=37.2549019607843, Blast_Score=94, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24645726, Length=166, Percent_Identity=31.3253012048193, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_MYCLB (B8ZSF6)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503932.1
- ProteinModelPortal:   B8ZSF6
- SMR:   B8ZSF6
- EnsemblBacteria:   EBMYCT00000087095
- GeneID:   7325543
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016783
- HOGENOM:   HBG665227
- OMA:   FVYDCAD
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21144; Mature: 21013

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: ACT_SITE 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAPIRIVGDPVLHTPTAPVQVAADGSLPANLNGLISTMYDTMDAAHGVGLAANQIGYG
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
LRVFVYDCAEDCRQTARRRGVVINPILETSEIPETMPDPDTDNEGCLSVPGESFPIGRAQ
EEEEEHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCH
WARVTGLDADGNPVTTEGTGLFARMLQHETGHLDGFLYLDYLIGRHARSAKRAIKSRHWG
HHEEECCCCCCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VPGLSWMPGEVPDPFGP
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AIAPIRIVGDPVLHTPTAPVQVAADGSLPANLNGLISTMYDTMDAAHGVGLAANQIGYG
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCC
LRVFVYDCAEDCRQTARRRGVVINPILETSEIPETMPDPDTDNEGCLSVPGESFPIGRAQ
EEEEEHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCH
WARVTGLDADGNPVTTEGTGLFARMLQHETGHLDGFLYLDYLIGRHARSAKRAIKSRHWG
HHEEECCCCCCCEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
VPGLSWMPGEVPDPFGP
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA