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The map label for this gene is ddl

Identifier: 221230366

GI number: 221230366

Start: 2021746

End: 2022900

Strand: Reverse

Name: ddl

Synonym: MLBr_01678

Alternate gene names: 221230366

Gene position: 2022900-2021746 (Counterclockwise)

Preceding gene: 221230367

Following gene: 221230364

Centisome position: 61.9

GC content: 61.82

Gene sequence:

>1155_bases
GTGAATGTCTACCAGCGGCCCTTGATGCCTTGGCTGCCATCCGGCGGCCGTGTGCGCGTTGCCGTCGTATTCGGCGGGCG
CAGTAACGAACACGCCGTCTCCTGTCTGTCTGCCGGCAGCATCTTGCGCAATCTGGATCGACAGCGGTTTGACGTGGTCG
CGGTTGGTATCACTCCGGAAGGGACGTGGATGCTCACCGATCCCAACCCGGATGCGCTGGCGATTGCCAATAGACAGCTT
CCCGCTGTGAGCTGCGAATCGGGAATCGAGTTGACGCTGCCGGCCGATCCACGGCGCAGCGGTCAACTGGTGTCACTTTC
ACACGGCGCCGGCCTTGGGGCCGGAGAGGTCCTGACGTCGGTCGATGTGGTATTCCCGGTGTTACATGGTCCATATGGCG
AGGACGGCACCATCCAGGGGCTGCTGGAACTCGCTCGTGTGCCCTACGTCGGAGCTGGTGTTCTGGCCAGCGCCGCCGGC
ATGGACAAGGAATTCACTAAGAAGCTCTTCGCTGCGGAGGGACTGCCGTTGAGCGCGTACACGGTGTTGCGTCCGTTGCG
GCCAACGCTGCATCGGCAGGAGTGTGAGCGGTTGAGTTTACCGGTATTCGTTAAACCCGCACGCGGTGGCTCGTCTATCG
GTATCAGCCGGGTGTCGAGCTGGGGTCAACTGCCCTCCGCGATTGCCTACGCTCGCCGGCACGATCCGAAGGTGATTGTC
GAGGCGGCGGTTAACGGCCGCGAACTGGAGTGCGGCGTGCTCGAAATGCCGGATGGCACTGTGGCAGCCAGCGCGGTGGG
GGAAATTCGGGTAGCCGGGGTGAGCGGAAGCGAGGATTCGTTCTACGACTTCACCACCAAATATCTCGACGACGCAGCCG
AGTTAGATGTGCCCGCCAAGGTGGACGATGACGTTGCTGACGAGGTGCGCCAGCTCGCGATTCAGGCGTTTCGGGCTATC
GACTGCCAGGGCCTGGCACGAGTTGACTTTTTCCTAACCGACGACGGGCCGGTGATCAATGAGATCAACACGATGCCGGG
GTTCACCATGACCTCGATGTACCCAAAGCTGTGGGCGGTCAGTAGCGTGGACTACCCGACGTTGCTAGCGACCATGGTGG
ATACGGCATTGGCTCGGGGTGTGGGTATGCGTTAG

Upstream 100 bases:

>100_bases
TGTGACGGGGCCCGCCCAGGTGGACCGTGAGTAGCCTAGAAGGCCTAGTTCAGAGTTTTAGCTGCTCTTGGAGCTGCTTG
ACCGGTAGCCTCTTTAGGCT

Downstream 100 bases:

>100_bases
CATGCTCGCTAGTGAGACTGAGCCGGGCTGATCGGGATGGCCGCCAAGGTCCGGTCGATCACGTCGGAGAGTTCTTGGAT
GGGTGTCGGTCCCGATCCAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MNVYQRPLMPWLPSGGRVRVAVVFGGRSNEHAVSCLSAGSILRNLDRQRFDVVAVGITPEGTWMLTDPNPDALAIANRQL
PAVSCESGIELTLPADPRRSGQLVSLSHGAGLGAGEVLTSVDVVFPVLHGPYGEDGTIQGLLELARVPYVGAGVLASAAG
MDKEFTKKLFAAEGLPLSAYTVLRPLRPTLHRQECERLSLPVFVKPARGGSSIGISRVSSWGQLPSAIAYARRHDPKVIV
EAAVNGRELECGVLEMPDGTVAASAVGEIRVAGVSGSEDSFYDFTTKYLDDAAELDVPAKVDDDVADEVRQLAIQAFRAI
DCQGLARVDFFLTDDGPVINEINTMPGFTMTSMYPKLWAVSSVDYPTLLATMVDTALARGVGMR

Sequences:

>Translated_384_residues
MNVYQRPLMPWLPSGGRVRVAVVFGGRSNEHAVSCLSAGSILRNLDRQRFDVVAVGITPEGTWMLTDPNPDALAIANRQL
PAVSCESGIELTLPADPRRSGQLVSLSHGAGLGAGEVLTSVDVVFPVLHGPYGEDGTIQGLLELARVPYVGAGVLASAAG
MDKEFTKKLFAAEGLPLSAYTVLRPLRPTLHRQECERLSLPVFVKPARGGSSIGISRVSSWGQLPSAIAYARRHDPKVIV
EAAVNGRELECGVLEMPDGTVAASAVGEIRVAGVSGSEDSFYDFTTKYLDDAAELDVPAKVDDDVADEVRQLAIQAFRAI
DCQGLARVDFFLTDDGPVINEINTMPGFTMTSMYPKLWAVSSVDYPTLLATMVDTALARGVGMR
>Mature_384_residues
MNVYQRPLMPWLPSGGRVRVAVVFGGRSNEHAVSCLSAGSILRNLDRQRFDVVAVGITPEGTWMLTDPNPDALAIANRQL
PAVSCESGIELTLPADPRRSGQLVSLSHGAGLGAGEVLTSVDVVFPVLHGPYGEDGTIQGLLELARVPYVGAGVLASAAG
MDKEFTKKLFAAEGLPLSAYTVLRPLRPTLHRQECERLSLPVFVKPARGGSSIGISRVSSWGQLPSAIAYARRHDPKVIV
EAAVNGRELECGVLEMPDGTVAASAVGEIRVAGVSGSEDSFYDFTTKYLDDAAELDVPAKVDDDVADEVRQLAIQAFRAI
DCQGLARVDFFLTDDGPVINEINTMPGFTMTSMYPKLWAVSSVDYPTLLATMVDTALARGVGMR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=368, Percent_Identity=42.6630434782609, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1786280, Length=261, Percent_Identity=33.7164750957854, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_MYCLE (Q9CBS0)

Other databases:

- EMBL:   AL583923
- PIR:   H87118
- RefSeq:   NP_302152.1
- ProteinModelPortal:   Q9CBS0
- EnsemblBacteria:   EBMYCT00000028141
- GeneID:   910044
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1678
- NMPDR:   fig|272631.1.peg.1024
- Leproma:   ML1678
- GeneTree:   EBGT00050000015718
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   MLEP272631:ML1678-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40981; Mature: 40981

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVYQRPLMPWLPSGGRVRVAVVFGGRSNEHAVSCLSAGSILRNLDRQRFDVVAVGITPE
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GTWMLTDPNPDALAIANRQLPAVSCESGIELTLPADPRRSGQLVSLSHGAGLGAGEVLTS
CCEEEECCCCCEEEEECCCCCCEECCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHH
VDVVFPVLHGPYGEDGTIQGLLELARVPYVGAGVLASAAGMDKEFTKKLFAAEGLPLSAY
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
TVLRPLRPTLHRQECERLSLPVFVKPARGGSSIGISRVSSWGQLPSAIAYARRHDPKVIV
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEE
EAAVNGRELECGVLEMPDGTVAASAVGEIRVAGVSGSEDSFYDFTTKYLDDAAELDVPAK
EECCCCCEEEEEEEECCCCCEEHHCCCEEEEEECCCCCCCHHHHHHHHHCHHHHCCCCCC
VDDDVADEVRQLAIQAFRAIDCQGLARVDFFLTDDGPVINEINTMPGFTMTSMYPKLWAV
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEHHCCCCCCCCHHHHCCHHEEE
SSVDYPTLLATMVDTALARGVGMR
CCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVYQRPLMPWLPSGGRVRVAVVFGGRSNEHAVSCLSAGSILRNLDRQRFDVVAVGITPE
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GTWMLTDPNPDALAIANRQLPAVSCESGIELTLPADPRRSGQLVSLSHGAGLGAGEVLTS
CCEEEECCCCCEEEEECCCCCCEECCCCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHH
VDVVFPVLHGPYGEDGTIQGLLELARVPYVGAGVLASAAGMDKEFTKKLFAAEGLPLSAY
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
TVLRPLRPTLHRQECERLSLPVFVKPARGGSSIGISRVSSWGQLPSAIAYARRHDPKVIV
HHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEE
EAAVNGRELECGVLEMPDGTVAASAVGEIRVAGVSGSEDSFYDFTTKYLDDAAELDVPAK
EECCCCCEEEEEEEECCCCCEEHHCCCEEEEEECCCCCCCHHHHHHHHHCHHHHCCCCCC
VDDDVADEVRQLAIQAFRAIDCQGLARVDFFLTDDGPVINEINTMPGFTMTSMYPKLWAV
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEHHCCCCCCCCHHHHCCHHEEE
SSVDYPTLLATMVDTALARGVGMR
CCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002