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The map label for this gene is trmD

Identifier: 221230327

GI number: 221230327

Start: 1938665

End: 1939381

Strand: Reverse

Name: trmD

Synonym: MLBr_01615

Alternate gene names: 221230327

Gene position: 1939381-1938665 (Counterclockwise)

Preceding gene: 221230328

Following gene: 221230326

Centisome position: 59.34

GC content: 60.81

Gene sequence:

>717_bases
ATGAAAATCGATATTGTCACGATTTTCCCGGCTTACCTAGACCCGCTGCGGCAATCGTTGCCGGGCAAGGCGATTGAGTC
GGGCCTGGTCGATGTGCAGGTGCACGACCTGCGGCGGTGGACCCATGATGTACACCACTCGGTGGACGACGTGCCCTATG
GTGGTGGTCCGGGGATGGTGATGAAAGCGCCGGTATGGGGGGAAGCGTTAGATGAAATCTGTTTCGACGAGACACTGTTG
GTGATCCCCACCCCCGCTGGTGCGCTGTTCACCCAGGCCACCGCACAGTGCTGGAGCACCGAGAGACACCTGGTGTTCGC
CTGCGGTCGCTACGAGGGCATCGATCAGCGAGTCGTTGATGATGCAGTACGACGAATGCGAGTCGAAGAGGTTTCAATCG
GTGACTATGTGTTGCCGGGTGGCGAGTCAGCGGCCGTGGTAATGATCGAGGCTGTTCTGCGCCTGGTGGCTGGGGTGCTC
GGCAATCCTGCGTCGCACCGCGATGATTCACACTCGCCGGACCTGGGTCGACTCTTGGAGGGGCCGAGCTATACTCGCCC
ACCTACCTGGCGTGGTCTCGACGTTCCGCCGGTTTTGTTGTCTGGTGATCATGCTCGAATCGCCGCTTGGCGCCGAGAGG
CTTCGCTCCGGAGAACTCGTAAACGCCGTCCCGATTTGTCGGGGGCCCGGTTTGTCTGGGGGCTGGGTTTGTCGTGA

Upstream 100 bases:

>100_bases
CCGGCGAAGTACTAGTGCCGTTTGTCGGTGCGATTGTCAAGTTGGTGTCTCTAGACGACGGCATAGTCGAAATCGACCCG
CCCAAGGGCCTGCTGGATCT

Downstream 100 bases:

>100_bases
AGTAAGCTAATCGTCAGATCCGGCCGTTGAGAAATATCGTCTTCGCAGCCTAGGTGATCGTAGGTCGCCCGGTGACATCA
CCGGATGGCTGCAACGACTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKIDIVTIFPAYLDPLRQSLPGKAIESGLVDVQVHDLRRWTHDVHHSVDDVPYGGGPGMVMKAPVWGEALDEICFDETLL
VIPTPAGALFTQATAQCWSTERHLVFACGRYEGIDQRVVDDAVRRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLVAGVL
GNPASHRDDSHSPDLGRLLEGPSYTRPPTWRGLDVPPVLLSGDHARIAAWRREASLRRTRKRRPDLSGARFVWGLGLS

Sequences:

>Translated_238_residues
MKIDIVTIFPAYLDPLRQSLPGKAIESGLVDVQVHDLRRWTHDVHHSVDDVPYGGGPGMVMKAPVWGEALDEICFDETLL
VIPTPAGALFTQATAQCWSTERHLVFACGRYEGIDQRVVDDAVRRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLVAGVL
GNPASHRDDSHSPDLGRLLEGPSYTRPPTWRGLDVPPVLLSGDHARIAAWRREASLRRTRKRRPDLSGARFVWGLGLS
>Mature_238_residues
MKIDIVTIFPAYLDPLRQSLPGKAIESGLVDVQVHDLRRWTHDVHHSVDDVPYGGGPGMVMKAPVWGEALDEICFDETLL
VIPTPAGALFTQATAQCWSTERHLVFACGRYEGIDQRVVDDAVRRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLVAGVL
GNPASHRDDSHSPDLGRLLEGPSYTRPPTWRGLDVPPVLLSGDHARIAAWRREASLRRTRKRRPDLSGARFVWGLGLS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=230, Percent_Identity=42.1739130434783, Blast_Score=164, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_MYCLE (O33017)

Other databases:

- EMBL:   Z97369
- EMBL:   AL583922
- PIR:   A87111
- RefSeq:   NP_302113.1
- ProteinModelPortal:   O33017
- SMR:   O33017
- EnsemblBacteria:   EBMYCT00000028900
- GeneID:   909818
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1615
- NMPDR:   fig|272631.1.peg.985
- Leproma:   ML1615
- GeneTree:   EBGT00050000016420
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   MLEP272631:ML1615-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26229; Mature: 26229

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDIVTIFPAYLDPLRQSLPGKAIESGLVDVQVHDLRRWTHDVHHSVDDVPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCCHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKAPVWGEALDEICFDETLLVIPTPAGALFTQATAQCWSTERHLVFACGRYEGIDQRVVD
EECCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
DAVRRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLVAGVLGNPASHRDDSHSPDLGRLLE
HHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
GPSYTRPPTWRGLDVPPVLLSGDHARIAAWRREASLRRTRKRRPDLSGARFVWGLGLS
CCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKIDIVTIFPAYLDPLRQSLPGKAIESGLVDVQVHDLRRWTHDVHHSVDDVPYGGGPGMV
CEEEEEEECHHHHHHHHHHCCCHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKAPVWGEALDEICFDETLLVIPTPAGALFTQATAQCWSTERHLVFACGRYEGIDQRVVD
EECCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
DAVRRMRVEEVSIGDYVLPGGESAAVVMIEAVLRLVAGVLGNPASHRDDSHSPDLGRLLE
HHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
GPSYTRPPTWRGLDVPPVLLSGDHARIAAWRREASLRRTRKRRPDLSGARFVWGLGLS
CCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002