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The map label for this gene is proS

Identifier: 221230293

GI number: 221230293

Start: 1877167

End: 1878609

Strand: Direct

Name: proS

Synonym: MLBr_01553

Alternate gene names: 221230293

Gene position: 1877167-1878609 (Clockwise)

Preceding gene: 221230282

Following gene: 221230298

Centisome position: 57.44

GC content: 59.25

Gene sequence:

>1443_bases
ATGGGATGCAACAAGCGAGGCGTGACTCGCCAGGAAGACGACTTCTCCGCCTGGTACAACGAGGTCGTCGCCAAGGCAGG
ACTGATCGATCGAGGACCCGCCAAGGGAACCATGGTGATTCGCCCGTACGGCTACCGAATTTGGGAGCTGTTGCAGGACG
AGCTTGATAGCAAGATCAAGGAACTCGGGCACGAAAATGCCTACTTTCCGATGCTGATCCCAGAGAACTATTTCAACCGG
GAAGCCGAGCATGTCGAAGGGTTCCATCCGGAACTGGCGGTCGTCACCCATGCCGGCGGCAAGGAGCTGTCCGAGCCCCT
CGTGATCCGTCCCACCTCCGAGACCGTCATCGGCGACATGATGGCCAAATGGATCACCTCCCACCGTGACCTACCGCTGC
GGTTAAACCAGTGGTCGAATGTGGTCCGTTGGGAACTTCGGCCCCGAATGCTGCTGCGCACGATCGAATTCCTGTGGCAA
GAAGGTCACTCGGCGCACATCGAGAAATCCGACGCTCTCCGCGAAACTTTGTTCGCTCTGGACATATACACCACGTTGGC
TCGTGACATGGCCGCCATTCCGATTGTTTCTGGAGAGAAAACCGCCGGTGAGCGGTTCGCCGGTGCGCTCAACACCTACA
CTATCGAAGCGATGATGCGCGATGGGAAAGCGTTGCAATCCGCAACCACTCATTACTTGGGAGACAACTTCGCTCGGGCG
TTCAACATCCGCTACACCACCGCCGAAGAACAACAAGCTTTCGTGCACACGACGTCTTTTGGGCTAAGTACGCGGATGAT
CGGTGCTATCGTGATGGTCCACGGCGATGACAAGGGCCTTGTTCTCCCACCCCAGGTGGCCCCCTACCAAGTTGTCATCG
TGCCAATCACTACCGGCAACAAGGCATCCGAGGTCGGACATGCCGCAGCAGACTTGGCGCGACGCCTGCAAGCTGCCGGG
GTGCGCACGCATGTGGATGCGCGTCCACAGCTGACGCCAGGCTGGAAATACAACGAATGGGAGCTCCGCGGGGTACCGAT
CCGCCTCGAACTCGGCCCGCGCGACCTCGAAGCCGGAACCACAGTCATGGTCAGGCGTATCGGTGAGAAAGCCAAACAGC
CCATTGCCATCGCAGCTGCCCCGACCGAACTCCCCGGCATTCTCGAAGAGTTCCAACGCACCTTGCTGGAGCGCGCCACC
CAATTCCGCGACGATCACACCACACTGGTCGACAACTGGGACGCTTTCGCAACAACCGTAGCCACAGGATGGGCACTTGC
CATTCATTGCGGCAACCCGGAGTGCGAAGAGGACATCAAAGCCGAAACAGCGGCCACCCCCCGATGCGTACCTCGGCAAG
GCGCCCCAGCTACCGGACGGTGCATACGCTGCGATGCACCATCTGCTTACGACAAGCGAGTCATCTTTGCGAGAGCCTAT
TGA

Upstream 100 bases:

>100_bases
GAGGCAACATCAACACCGCCATTATTACGCACGACCGGATGAAGCCGTCCATAGACCGCTACATTCCGGCAACGAGGTCG
GCAGGTAGACTGCGCGATCC

Downstream 100 bases:

>100_bases
CCGGACGGCCCTGGCCGGCACATCGTTAGTCAAACGCTGCGCGCAGTGCAGTTCACCACATCATCGATCAGACCCTTGGT
CGAATAGCCGGTGGCCACCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MGCNKRGVTRQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQDELDSKIKELGHENAYFPMLIPENYFNR
EAEHVEGFHPELAVVTHAGGKELSEPLVIRPTSETVIGDMMAKWITSHRDLPLRLNQWSNVVRWELRPRMLLRTIEFLWQ
EGHSAHIEKSDALRETLFALDIYTTLARDMAAIPIVSGEKTAGERFAGALNTYTIEAMMRDGKALQSATTHYLGDNFARA
FNIRYTTAEEQQAFVHTTSFGLSTRMIGAIVMVHGDDKGLVLPPQVAPYQVVIVPITTGNKASEVGHAAADLARRLQAAG
VRTHVDARPQLTPGWKYNEWELRGVPIRLELGPRDLEAGTTVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLLERAT
QFRDDHTTLVDNWDAFATTVATGWALAIHCGNPECEEDIKAETAATPRCVPRQGAPATGRCIRCDAPSAYDKRVIFARAY

Sequences:

>Translated_480_residues
MGCNKRGVTRQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQDELDSKIKELGHENAYFPMLIPENYFNR
EAEHVEGFHPELAVVTHAGGKELSEPLVIRPTSETVIGDMMAKWITSHRDLPLRLNQWSNVVRWELRPRMLLRTIEFLWQ
EGHSAHIEKSDALRETLFALDIYTTLARDMAAIPIVSGEKTAGERFAGALNTYTIEAMMRDGKALQSATTHYLGDNFARA
FNIRYTTAEEQQAFVHTTSFGLSTRMIGAIVMVHGDDKGLVLPPQVAPYQVVIVPITTGNKASEVGHAAADLARRLQAAG
VRTHVDARPQLTPGWKYNEWELRGVPIRLELGPRDLEAGTTVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLLERAT
QFRDDHTTLVDNWDAFATTVATGWALAIHCGNPECEEDIKAETAATPRCVPRQGAPATGRCIRCDAPSAYDKRVIFARAY
>Mature_479_residues
GCNKRGVTRQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQDELDSKIKELGHENAYFPMLIPENYFNRE
AEHVEGFHPELAVVTHAGGKELSEPLVIRPTSETVIGDMMAKWITSHRDLPLRLNQWSNVVRWELRPRMLLRTIEFLWQE
GHSAHIEKSDALRETLFALDIYTTLARDMAAIPIVSGEKTAGERFAGALNTYTIEAMMRDGKALQSATTHYLGDNFARAF
NIRYTTAEEQQAFVHTTSFGLSTRMIGAIVMVHGDDKGLVLPPQVAPYQVVIVPITTGNKASEVGHAAADLARRLQAAGV
RTHVDARPQLTPGWKYNEWELRGVPIRLELGPRDLEAGTTVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLLERATQ
FRDDHTTLVDNWDAFATTVATGWALAIHCGNPECEEDIKAETAATPRCVPRQGAPATGRCIRCDAPSAYDKRVIFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=500, Percent_Identity=42.2, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1786392, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71991342, Length=499, Percent_Identity=41.4829659318637, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71991346, Length=354, Percent_Identity=48.8700564971751, Blast_Score=355, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6321808, Length=498, Percent_Identity=40.7630522088353, Blast_Score=396, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24649468, Length=470, Percent_Identity=42.1276595744681, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24649466, Length=470, Percent_Identity=42.1276595744681, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_MYCLB (B8ZRT2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503709.1
- ProteinModelPortal:   B8ZRT2
- SMR:   B8ZRT2
- EnsemblBacteria:   EBMYCT00000087584
- GeneID:   7325213
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016802
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 53586; Mature: 53454

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCNKRGVTRQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQDELDSKIK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
ELGHENAYFPMLIPENYFNREAEHVEGFHPELAVVTHAGGKELSEPLVIRPTSETVIGDM
HCCCCCCEEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCHHCCCEEEECCCHHHHHHH
MAKWITSHRDLPLRLNQWSNVVRWELRPRMLLRTIEFLWQEGHSAHIEKSDALRETLFAL
HHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
DIYTTLARDMAAIPIVSGEKTAGERFAGALNTYTIEAMMRDGKALQSATTHYLGDNFARA
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHEEHHHHHHCCHHHHHHHHHHHCCCCEEE
FNIRYTTAEEQQAFVHTTSFGLSTRMIGAIVMVHGDDKGLVLPPQVAPYQVVIVPITTGN
EEEEEECCCCCCEEEEEECCCCHHEEEEEEEEEEECCCCEEECCCCCCEEEEEEEEECCC
KASEVGHAAADLARRLQAAGVRTHVDARPQLTPGWKYNEWELRGVPIRLELGPRDLEAGT
CHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCCCH
TVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLLERATQFRDDHTTLVDNWDAFATTV
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
ATGWALAIHCGNPECEEDIKAETAATPRCVPRQGAPATGRCIRCDAPSAYDKRVIFARAY
HCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
GCNKRGVTRQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQDELDSKIK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
ELGHENAYFPMLIPENYFNREAEHVEGFHPELAVVTHAGGKELSEPLVIRPTSETVIGDM
HCCCCCCEEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCHHCCCEEEECCCHHHHHHH
MAKWITSHRDLPLRLNQWSNVVRWELRPRMLLRTIEFLWQEGHSAHIEKSDALRETLFAL
HHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
DIYTTLARDMAAIPIVSGEKTAGERFAGALNTYTIEAMMRDGKALQSATTHYLGDNFARA
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHEEHHHHHHCCHHHHHHHHHHHCCCCEEE
FNIRYTTAEEQQAFVHTTSFGLSTRMIGAIVMVHGDDKGLVLPPQVAPYQVVIVPITTGN
EEEEEECCCCCCEEEEEECCCCHHEEEEEEEEEEECCCCEEECCCCCCEEEEEEEEECCC
KASEVGHAAADLARRLQAAGVRTHVDARPQLTPGWKYNEWELRGVPIRLELGPRDLEAGT
CHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEECEEEEEEECCCCCCCCH
TVMVRRIGEKAKQPIAIAAAPTELPGILEEFQRTLLERATQFRDDHTTLVDNWDAFATTV
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHH
ATGWALAIHCGNPECEEDIKAETAATPRCVPRQGAPATGRCIRCDAPSAYDKRVIFARAY
HCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA