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The map label for this gene is tig

Identifier: 221230256

GI number: 221230256

Start: 1783500

End: 1784909

Strand: Reverse

Name: tig

Synonym: MLBr_01481

Alternate gene names: 221230256

Gene position: 1784909-1783500 (Counterclockwise)

Preceding gene: 221230257

Following gene: 221230255

Centisome position: 54.62

GC content: 59.08

Gene sequence:

>1410_bases
GTGAAAAGCAGTGTCGAGCAGTTGAACCCTACTCGGGTGCGTATCAACGTGGAAGTACCTTTTACAGAGCTTGAGCCGGA
TTTTCAGCGGGCCTACAAAGAGCTGGCCAGACATGTGCAGCTGCCCGGTTTCCGGCCCGGGAAAGTTCCTGCGCGATTGC
TGGAGGCCCGTTTTGGTCGGGAGACGCTGCTCGATCAGGTCGTCAACGAAGCCATGCCCAGTCGGTACGGGCAGGCGCTG
GCGGAGTCTGAGGTCCAACCGATCGGCCAGCCCGAAATAGAGGTGATCAGAAAGGAGTACGGTCAGGATCTGGCTTTCAC
CGTCGAAGTTGAGGTACGCCCCAAGATCGCACTTCCGGATTTCAGCACATTGAAGGTTGTTGTGGATCCGGTCGAGGTCA
GCACCGATGATGTCGAGGCAGAGTTGCGGTCGTTGCGTGCCAGGTTCGGCACTCTGATCGGCGTGGACCGACCTGTGGCG
CTCGGTGACTTTGTCTCGATTGATTTGTCGGCCACCATCAATGGCGAGAAGGTGCCGAACGCCGATGCGGAGGGGCTGTC
TCATGAGGTCGGCTACGGCCGGCTTATCGCAGGTCTCGACGATGCGCTCGTCGGTTTGTCCGCTGGTGAGTCGCGAGTCT
TCACCACCCAGTTAGCGACCAGCAAACACGCCGGGCAAGACGCAGAGGTCATCGTCACCGTCAAGTCGGTCAAGGAACGC
GAATTGCCGGAGCCGGATGACGAATTCGCGCAACTGGTCAGCGAGTTCGACACCATGGCAGAACTACGGGCCAATCTCGG
GGATCAGGTCCGTAAGGCTAAGTACGCCCAGCAAGCGGAGAAAATCCGTGACGCGGCTGTGGACGCTTTGCTCGAGCGGG
TAGATGTGCCATTGCCGGAAGGGATTGTGCAAGCCCAGTTCAATAATGCCCTGCATGACGCGCTCAGCGGCCTGGGCCAC
GACGAAGCCAAGTTCGCCGAGGTGCTCGCTGAGCGTGGGTCGTCGCGCGAGGAGTTCGAGGCTGAAGCGCGTAGCGCTGC
CGAGAGAGATGTCACGAGGCAACTGCTGTTGGATGTAGTGGCTGACGACCAGAAGATCCAGGTGGGTCAGGATGACCTGA
ACGAACGCTTGCTCGCCACGTCTCAGCAATACGGCGTCGACGCACAGCAGCTGTTTGGCTTCTTGCGGGAAAACAACCGG
TTATCGTCCTTGGTTACTGACGCGCGGCGCAGGCTGGCGGTCGCAGCTGTGGTCGAGGCGGCTACGTTCACCGATAGTGA
CGGAAACACGATAGACACCAGTGAATTTTTCGGTAAGCATGCCCAAAGCGATAAGGCCGACCAGAAGACCGAAGAAGCAG
ATCCGAACAGTGATGCCATCGATGAAGAGGTCGACGAGGCCGCGGAATGA

Upstream 100 bases:

>100_bases
GCGCAGCTTGGTAGCGCATCCGCTTTGGGAGCGGAAGGCCGCAGGTTCAAATCCTGTCACCCCGACCAATCGCCAGGGAC
GACCACCGAGGAGCACGCCC

Downstream 100 bases:

>100_bases
CAATGACTGGGTTATGACGCTGTAAACGAACGTGCCCCTTTCAGGGGGTGCGTGATCGCCAAACAACGGGCTCGGTTCGT
TAGTGTCGGTGCATACAGAA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKSSVEQLNPTRVRINVEVPFTELEPDFQRAYKELARHVQLPGFRPGKVPARLLEARFGRETLLDQVVNEAMPSRYGQAL
AESEVQPIGQPEIEVIRKEYGQDLAFTVEVEVRPKIALPDFSTLKVVVDPVEVSTDDVEAELRSLRARFGTLIGVDRPVA
LGDFVSIDLSATINGEKVPNADAEGLSHEVGYGRLIAGLDDALVGLSAGESRVFTTQLATSKHAGQDAEVIVTVKSVKER
ELPEPDDEFAQLVSEFDTMAELRANLGDQVRKAKYAQQAEKIRDAAVDALLERVDVPLPEGIVQAQFNNALHDALSGLGH
DEAKFAEVLAERGSSREEFEAEARSAAERDVTRQLLLDVVADDQKIQVGQDDLNERLLATSQQYGVDAQQLFGFLRENNR
LSSLVTDARRRLAVAAVVEAATFTDSDGNTIDTSEFFGKHAQSDKADQKTEEADPNSDAIDEEVDEAAE

Sequences:

>Translated_469_residues
MKSSVEQLNPTRVRINVEVPFTELEPDFQRAYKELARHVQLPGFRPGKVPARLLEARFGRETLLDQVVNEAMPSRYGQAL
AESEVQPIGQPEIEVIRKEYGQDLAFTVEVEVRPKIALPDFSTLKVVVDPVEVSTDDVEAELRSLRARFGTLIGVDRPVA
LGDFVSIDLSATINGEKVPNADAEGLSHEVGYGRLIAGLDDALVGLSAGESRVFTTQLATSKHAGQDAEVIVTVKSVKER
ELPEPDDEFAQLVSEFDTMAELRANLGDQVRKAKYAQQAEKIRDAAVDALLERVDVPLPEGIVQAQFNNALHDALSGLGH
DEAKFAEVLAERGSSREEFEAEARSAAERDVTRQLLLDVVADDQKIQVGQDDLNERLLATSQQYGVDAQQLFGFLRENNR
LSSLVTDARRRLAVAAVVEAATFTDSDGNTIDTSEFFGKHAQSDKADQKTEEADPNSDAIDEEVDEAAE
>Mature_469_residues
MKSSVEQLNPTRVRINVEVPFTELEPDFQRAYKELARHVQLPGFRPGKVPARLLEARFGRETLLDQVVNEAMPSRYGQAL
AESEVQPIGQPEIEVIRKEYGQDLAFTVEVEVRPKIALPDFSTLKVVVDPVEVSTDDVEAELRSLRARFGTLIGVDRPVA
LGDFVSIDLSATINGEKVPNADAEGLSHEVGYGRLIAGLDDALVGLSAGESRVFTTQLATSKHAGQDAEVIVTVKSVKER
ELPEPDDEFAQLVSEFDTMAELRANLGDQVRKAKYAQQAEKIRDAAVDALLERVDVPLPEGIVQAQFNNALHDALSGLGH
DEAKFAEVLAERGSSREEFEAEARSAAERDVTRQLLLDVVADDQKIQVGQDDLNERLLATSQQYGVDAQQLFGFLRENNR
LSSLVTDARRRLAVAAVVEAATFTDSDGNTIDTSEFFGKHAQSDKADQKTEEADPNSDAIDEEVDEAAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=433, Percent_Identity=25.635103926097, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_MYCLB (B8ZRP5)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503672.1
- ProteinModelPortal:   B8ZRP5
- EnsemblBacteria:   EBMYCT00000087714
- GeneID:   7327275
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015814
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51477; Mature: 51477

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSVEQLNPTRVRINVEVPFTELEPDFQRAYKELARHVQLPGFRPGKVPARLLEARFGR
CCCCHHHCCCEEEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCH
ETLLDQVVNEAMPSRYGQALAESEVQPIGQPEIEVIRKEYGQDLAFTVEVEVRPKIALPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEEECCEEECCC
FSTLKVVVDPVEVSTDDVEAELRSLRARFGTLIGVDRPVALGDFVSIDLSATINGEKVPN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCC
ADAEGLSHEVGYGRLIAGLDDALVGLSAGESRVFTTQLATSKHAGQDAEVIVTVKSVKER
CCCCCCCHHCCCHHHHHCCHHHHHCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEEHHHHC
ELPEPDDEFAQLVSEFDTMAELRANLGDQVRKAKYAQQAEKIRDAAVDALLERVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GIVQAQFNNALHDALSGLGHDEAKFAEVLAERGSSREEFEAEARSAAERDVTRQLLLDVV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADDQKIQVGQDDLNERLLATSQQYGVDAQQLFGFLRENNRLSSLVTDARRRLAVAAVVEA
CCCCEEECCHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ATFTDSDGNTIDTSEFFGKHAQSDKADQKTEEADPNSDAIDEEVDEAAE
HHEECCCCCEECCHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKSSVEQLNPTRVRINVEVPFTELEPDFQRAYKELARHVQLPGFRPGKVPARLLEARFGR
CCCCHHHCCCEEEEEEEECCHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCH
ETLLDQVVNEAMPSRYGQALAESEVQPIGQPEIEVIRKEYGQDLAFTVEVEVRPKIALPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEEEEECCEEECCC
FSTLKVVVDPVEVSTDDVEAELRSLRARFGTLIGVDRPVALGDFVSIDLSATINGEKVPN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCC
ADAEGLSHEVGYGRLIAGLDDALVGLSAGESRVFTTQLATSKHAGQDAEVIVTVKSVKER
CCCCCCCHHCCCHHHHHCCHHHHHCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEEHHHHC
ELPEPDDEFAQLVSEFDTMAELRANLGDQVRKAKYAQQAEKIRDAAVDALLERVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GIVQAQFNNALHDALSGLGHDEAKFAEVLAERGSSREEFEAEARSAAERDVTRQLLLDVV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADDQKIQVGQDDLNERLLATSQQYGVDAQQLFGFLRENNRLSSLVTDARRRLAVAAVVEA
CCCCEEECCHHHHHHHHHHCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ATFTDSDGNTIDTSEFFGKHAQSDKADQKTEEADPNSDAIDEEVDEAAE
HHEECCCCCEECCHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA