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The map label for this gene is clpP [H]

Identifier: 221230255

GI number: 221230255

Start: 1782774

End: 1783448

Strand: Reverse

Name: clpP [H]

Synonym: MLBr_01480

Alternate gene names: 221230255

Gene position: 1783448-1782774 (Counterclockwise)

Preceding gene: 221230256

Following gene: 221230254

Centisome position: 54.57

GC content: 59.41

Gene sequence:

>675_bases
GTGATCGCCAAACAACGGGCTCGGTTCGTTAGTGTCGGTGCATACAGAACTCCAGAAAGCAGGTATCCCACTGTGGGCCA
CGTGACTGACATCCGTTCGAATTCGCAGGGTTTCAACCTCACGGACTCGGTCTATGAGCGATTGCTCTCCGAACGCATCA
TCTTCCTGGGGTCGGAGGTGAACGACAACATCGCCAATCGGCTCTGCGCACAGATACTGCTGCTTGCCGCCGAAGACGCC
TTCAAGGACATTTCTCTGTACATCAATTCCCCCGGTGGATCGATCAGCGCCGGGATGGCGATCTACGACACCATGGTGCT
GGCGCCATGTGACATCGCCACCTACGCGATGGGCATGGCCGCGTCGATGGGGGAGTTCTTGCTGGCGGCGGGAACTAGGG
GCAAGCGCTACGCGCTACCACACGCCCGCATCCTAATGCACCAGCCGCTGGGCGGGGTGACCGGCAGTGCAGCCGACATC
GCCATCCAGGCTGAGCAGTTCGCCGTCATCAAAAAGGAGATGTTCCGGCTCAACGCTGAGTTCACCGGTCAGCCGATCGA
GCGAATCGAAGCGGATTCTGACCGCGACCGCTGGTTCACTGCCTCGGAAGCCCTGGAATACGGTTTTGTCGACCACATCA
TTACCCGCGCCCACGTCAACGGAGAAGTGCAGTGA

Upstream 100 bases:

>100_bases
TCCGAACAGTGATGCCATCGATGAAGAGGTCGACGAGGCCGCGGAATGACAATGACTGGGTTATGACGCTGTAAACGAAC
GTGCCCCTTTCAGGGGGTGC

Downstream 100 bases:

>100_bases
AACTGCAGAATTCTCAGCTCCAACCTCAGGCGCGATACATCCTGCCATCATTCATCGAGCACTCCAGTTTCGGCGTCAAG
GAGTCCAACCCTTACAACAA

Product: ATP-dependent Clp protease proteolytic subunit

Products: NA

Alternate protein names: Endopeptidase Clp 1 [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIAKQRARFVSVGAYRTPESRYPTVGHVTDIRSNSQGFNLTDSVYERLLSERIIFLGSEVNDNIANRLCAQILLLAAEDA
FKDISLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMAASMGEFLLAAGTRGKRYALPHARILMHQPLGGVTGSAADI
AIQAEQFAVIKKEMFRLNAEFTGQPIERIEADSDRDRWFTASEALEYGFVDHIITRAHVNGEVQ

Sequences:

>Translated_224_residues
MIAKQRARFVSVGAYRTPESRYPTVGHVTDIRSNSQGFNLTDSVYERLLSERIIFLGSEVNDNIANRLCAQILLLAAEDA
FKDISLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMAASMGEFLLAAGTRGKRYALPHARILMHQPLGGVTGSAADI
AIQAEQFAVIKKEMFRLNAEFTGQPIERIEADSDRDRWFTASEALEYGFVDHIITRAHVNGEVQ
>Mature_224_residues
MIAKQRARFVSVGAYRTPESRYPTVGHVTDIRSNSQGFNLTDSVYERLLSERIIFLGSEVNDNIANRLCAQILLLAAEDA
FKDISLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMAASMGEFLLAAGTRGKRYALPHARILMHQPLGGVTGSAADI
AIQAEQFAVIKKEMFRLNAEFTGQPIERIEADSDRDRWFTASEALEYGFVDHIITRAHVNGEVQ

Specific function: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=179, Percent_Identity=45.8100558659218, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1786641, Length=185, Percent_Identity=53.5135135135135, Blast_Score=200, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17538017, Length=175, Percent_Identity=46.2857142857143, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI20129427, Length=171, Percent_Identity=45.0292397660819, Blast_Score=160, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907
- InterPro:   IPR018215 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: =3.4.21.92 [H]

Molecular weight: Translated: 24582; Mature: 24582

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00382 CLP_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKQRARFVSVGAYRTPESRYPTVGHVTDIRSNSQGFNLTDSVYERLLSERIIFLGSEV
CCCCCCCCEEEECCEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCC
NDNIANRLCAQILLLAAEDAFKDISLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMA
CHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ASMGEFLLAAGTRGKRYALPHARILMHQPLGGVTGSAADIAIQAEQFAVIKKEMFRLNAE
HHHHHHHHHCCCCCCEEECCHHHHEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
FTGQPIERIEADSDRDRWFTASEALEYGFVDHIITRAHVNGEVQ
CCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAKQRARFVSVGAYRTPESRYPTVGHVTDIRSNSQGFNLTDSVYERLLSERIIFLGSEV
CCCCCCCCEEEECCEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCC
NDNIANRLCAQILLLAAEDAFKDISLYINSPGGSISAGMAIYDTMVLAPCDIATYAMGMA
CHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ASMGEFLLAAGTRGKRYALPHARILMHQPLGGVTGSAADIAIQAEQFAVIKKEMFRLNAE
HHHHHHHHHCCCCCCEEECCHHHHEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
FTGQPIERIEADSDRDRWFTASEALEYGFVDHIITRAHVNGEVQ
CCCCHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]