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The map label for this gene is argG

Identifier: 221230219

GI number: 221230219

Start: 1693546

End: 1694745

Strand: Direct

Name: argG

Synonym: MLBr_01412

Alternate gene names: 221230219

Gene position: 1693546-1694745 (Clockwise)

Preceding gene: 221230218

Following gene: 221230220

Centisome position: 51.82

GC content: 58.83

Gene sequence:

>1200_bases
TTGATGCCGGAACGTATTATCCTGGCGTATTCGGGGGGGCTGGATACCTCGGTGGCGATCAACTGGATCGGCAAGGAAAC
TAGCCATGAGGTCGTGGCAGTGGTGATTGATCTCGGCCAGGGCGGCGAGGACATGGAGGTCGTACGTCAACGGGCGTTGG
ACTGCGGAGCTGTTGAGGCTATCGTTGTCGATGCCCGTGACGAGTTCGCTGAAGGCTATTGCTTGCCCACCGTTTTGAAC
AACGCGCTGTATATGGATCGGTACCCGTTGGTGTCCGCGATCAGCAGGCCGCTGATCGTCAAGCACCTGGTCGCGGCCGC
ACGTGCGCACGGCGGCAGCATCGTCGCGCACGGTTGTACCGGCAAGGGTAACGACCAGGTCCGATTCGAAGTCGGATTCG
CTTCGCTGGCACCGGATCTCGAAATATTGGCGCCGGTGCGCGACTACGCATGGACACGAGAGAAGGCGATCGCTTTCGCC
GAAGAGAACGCCATCCCCATCAATGTCACCAAACGTTCGCCGTTCTCGATTGACCAGAATGTTTGGGGCCGCGCAGTGGA
AACCGGTTTCCTGGAACACTTGTGGCACGCACCTACTAAAGAGGTTTATTCCTACACTGACGACCCCACCATCAATTGGA
ACACGCCCGATGAGGTGATCGTCGGTTTTGAGCACGGAGTTCCGGTGTCGATTGACGGTAGTCCGGTGTCCATGCTGGGC
GCCATCGAGGCACTCAACCGACGCGCTGGCGCGCAAGGCGTCGGGCGTCTCGACGTTGTCGAAGACCGGCTGGTAGGCAT
CAAGAGCCGCGAGATTTACGAGGCACCCGGAGCAATGGTGCTCATCACCGCGCACGCCGAACTCGAGCACGTCACGCTGG
AGCGTGAGTTGGGACGCTTCAAGCGTCAGACCGATCGACGCTGGGCCGAATTGGTGTATGACGGATTGTGGTATTCGCCG
CTGAAGACCGCACTGGAGAGTTTTGTCGCCGCTACGCAACAACACGTTACCGGTGAAGTTCGAATGGTGTTACATGGAGG
CCATATTGCGGTGAATGGGCGGCGCAGCGCCGAATCCCTATACGATTTCAATTTGGCCACCTACGACGAAGGGGATACCT
TCGATCAATCCGCTGCTCGCGGCTTCGTCTACGTGTACGGGTTACCGTCGAAGCTCGCGGCACGTAGGGACTTACGGTGA

Upstream 100 bases:

>100_bases
CATCGCTGGTGACGACACCGTCTTCGTGGCCGCCCGCGAGCCGATGACTGGCTCCGAGTTGGCCACTGTTCTCGAAAGCC
TGAATAGATAAGGAGATTGG

Downstream 100 bases:

>100_bases
GGTAAGAGTGTGATATTGAGCGCCCCGACAAGGCGTCGTCAGATCCACACTTGGGGTGAAATGACAGGCCAATGAGCACC
AATCAGGGTTCGCTGTGGGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MMPERIILAYSGGLDTSVAINWIGKETSHEVVAVVIDLGQGGEDMEVVRQRALDCGAVEAIVVDARDEFAEGYCLPTVLN
NALYMDRYPLVSAISRPLIVKHLVAAARAHGGSIVAHGCTGKGNDQVRFEVGFASLAPDLEILAPVRDYAWTREKAIAFA
EENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWHAPTKEVYSYTDDPTINWNTPDEVIVGFEHGVPVSIDGSPVSMLG
AIEALNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHAELEHVTLERELGRFKRQTDRRWAELVYDGLWYSP
LKTALESFVAATQQHVTGEVRMVLHGGHIAVNGRRSAESLYDFNLATYDEGDTFDQSAARGFVYVYGLPSKLAARRDLR

Sequences:

>Translated_399_residues
MMPERIILAYSGGLDTSVAINWIGKETSHEVVAVVIDLGQGGEDMEVVRQRALDCGAVEAIVVDARDEFAEGYCLPTVLN
NALYMDRYPLVSAISRPLIVKHLVAAARAHGGSIVAHGCTGKGNDQVRFEVGFASLAPDLEILAPVRDYAWTREKAIAFA
EENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWHAPTKEVYSYTDDPTINWNTPDEVIVGFEHGVPVSIDGSPVSMLG
AIEALNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHAELEHVTLERELGRFKRQTDRRWAELVYDGLWYSP
LKTALESFVAATQQHVTGEVRMVLHGGHIAVNGRRSAESLYDFNLATYDEGDTFDQSAARGFVYVYGLPSKLAARRDLR
>Mature_399_residues
MMPERIILAYSGGLDTSVAINWIGKETSHEVVAVVIDLGQGGEDMEVVRQRALDCGAVEAIVVDARDEFAEGYCLPTVLN
NALYMDRYPLVSAISRPLIVKHLVAAARAHGGSIVAHGCTGKGNDQVRFEVGFASLAPDLEILAPVRDYAWTREKAIAFA
EENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWHAPTKEVYSYTDDPTINWNTPDEVIVGFEHGVPVSIDGSPVSMLG
AIEALNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHAELEHVTLERELGRFKRQTDRRWAELVYDGLWYSP
LKTALESFVAATQQHVTGEVRMVLHGGHIAVNGRRSAESLYDFNLATYDEGDTFDQSAARGFVYVYGLPSKLAARRDLR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=393, Percent_Identity=37.9134860050891, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI16950633, Length=393, Percent_Identity=37.9134860050891, Blast_Score=261, Evalue=6e-70,
Organism=Escherichia coli, GI1789563, Length=373, Percent_Identity=30.2949061662198, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=34.1584158415842, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI21358151, Length=399, Percent_Identity=36.3408521303258, Blast_Score=254, Evalue=9e-68,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_MYCLE (Q9CC10)

Other databases:

- EMBL:   AL583922
- PIR:   F87085
- RefSeq:   NP_302005.1
- ProteinModelPortal:   Q9CC10
- SMR:   Q9CC10
- EnsemblBacteria:   EBMYCT00000029177
- GeneID:   910554
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1412
- NMPDR:   fig|272631.1.peg.877
- Leproma:   ML1412
- GeneTree:   EBGT00050000017658
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   MLEP272631:ML1412-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 43916; Mature: 43916

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 88-88 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 176-176 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPERIILAYSGGLDTSVAINWIGKETSHEVVAVVIDLGQGGEDMEVVRQRALDCGAVEA
CCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEE
IVVDARDEFAEGYCLPTVLNNALYMDRYPLVSAISRPLIVKHLVAAARAHGGSIVAHGCT
EEEECCHHHHCCEEHHHHHCCCCEECCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCC
GKGNDQVRFEVGFASLAPDLEILAPVRDYAWTREKAIAFAEENAIPINVTKRSPFSIDQN
CCCCCEEEEEECHHHHCCCHHEEHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCHH
VWGRAVETGFLEHLWHAPTKEVYSYTDDPTINWNTPDEVIVGFEHGVPVSIDGSPVSMLG
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCCCCCCEEEEECCCCEEEECCCCHHHHH
AIEALNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHAELEHVTLERELGRF
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
KRQTDRRWAELVYDGLWYSPLKTALESFVAATQQHVTGEVRMVLHGGHIAVNGRRSAESL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHH
YDFNLATYDEGDTFDQSAARGFVYVYGLPSKLAARRDLR
HCCCEEECCCCCCCCHHHCCCEEEEEECCHHHHHHCCCC
>Mature Secondary Structure
MMPERIILAYSGGLDTSVAINWIGKETSHEVVAVVIDLGQGGEDMEVVRQRALDCGAVEA
CCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEE
IVVDARDEFAEGYCLPTVLNNALYMDRYPLVSAISRPLIVKHLVAAARAHGGSIVAHGCT
EEEECCHHHHCCEEHHHHHCCCCEECCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCC
GKGNDQVRFEVGFASLAPDLEILAPVRDYAWTREKAIAFAEENAIPINVTKRSPFSIDQN
CCCCCEEEEEECHHHHCCCHHEEHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCHH
VWGRAVETGFLEHLWHAPTKEVYSYTDDPTINWNTPDEVIVGFEHGVPVSIDGSPVSMLG
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCCCCCCEEEEECCCCEEEECCCCHHHHH
AIEALNRRAGAQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHAELEHVTLERELGRF
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
KRQTDRRWAELVYDGLWYSPLKTALESFVAATQQHVTGEVRMVLHGGHIAVNGRRSAESL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHH
YDFNLATYDEGDTFDQSAARGFVYVYGLPSKLAARRDLR
HCCCEEECCCCCCCCHHHCCCEEEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002