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The map label for this gene is sigB [H]

Identifier: 221229964

GI number: 221229964

Start: 1184370

End: 1185329

Strand: Reverse

Name: sigB [H]

Synonym: MLBr_01014

Alternate gene names: 221229964

Gene position: 1185329-1184370 (Counterclockwise)

Preceding gene: 221229965

Following gene: 221229963

Centisome position: 36.27

GC content: 59.79

Gene sequence:

>960_bases
ATGGCCGAAGCCACCACAAACCGGGTTGACAGCGATCTGGATGCTCAAAGCCCCGCCGCGGACCTGGTGCGTGTGTATCT
GAACGGCATCGGTAAGACAGCGTTGCTCAACGCCGCCGATGAAGTCGAATTGGCCAAACGCATCGAAGCTGGGCTCTATG
CGGAGCATCTGCTCCAAACTCGCAAGCGCTTCGGCGAGGGCCGCAAACGCGCATTAGCAGCTGTGGCGCGCGATGGCGCA
GCAGCACGTCGCCACCTGCTGGAAGCTAATTTACGCCTTGTTGTTTCGCTGGCCAAGCGGTACACGGGTCGGGGAATGCC
ATTGCTGGACCTAATCCAGGAGGGCAACCTGGGGTTGATCCGCGCGATGGAGAAGTTTGACTACACAAAGGGATTCAAGT
TTTCGACGTATGCTACTTGGTGGATCCGACAGGCCATCACCCGCGGTATGGCCGACCAGAGCCGCACCATCCGACTGCCG
GTGCACTTAGTTGAGCAAGTCAACAAGCTGGCCCGGATCAAGCGGGAAATGCACCAAAACCTAGGACGGGAAGCTACTGA
CGAAGAACTCGCCGCTGAATCGGGAATTCCAATCGAGAAGATCAACGACCTGCTGGAACACAGTCGCGACCCGGTCAGTC
TGGACATGCCGGTCGGCTCCGAGGAGGAGGCCCCGCTGGGCGACTTCATCGAAGACGCCGAAGCCATGTCCGCGGAGAAC
GCAGTCATCGCCGAACTTCTGCACACCGATATCCGCAGTGTGCTGGCCACTCTCGACGAACGTGAACACCAGGTGATCCG
GCTGCGTTTCGGTCTAGACGACGGCCAGCCACGCACCCTGGATCAGATCGGCAAGCTGTTCGGTTTGTCCCGCGAGCGGG
TCCGCCAAATCGAGCGAGACGTGATGTGCAAGCTCCGCAACGGTGAGCGCGCTGACCGACTACGCTCGTACGCGAGTTAA

Upstream 100 bases:

>100_bases
TTCCGCACGTCAAAGCGTGTGAGATGACAGCAGGACGGGAACTCTCAAGGAGGACCCGTCGTTAAAACAAACTACAGAAC
AAGCCGATTGGGAGGGCGTC

Downstream 100 bases:

>100_bases
CACGCTACTTAGGCCACCCAGGTCAGGGCCGCCAGCCGAAGCCAGGTAACAGACAGTTATGCCCGCCGCCCCGTGGCGGG
CATACTGCTGCCGCGAAGCT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAEATTNRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAEHLLQTRKRFGEGRKRALAAVARDGA
AARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLP
VHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAEN
AVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMCKLRNGERADRLRSYAS

Sequences:

>Translated_319_residues
MAEATTNRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAEHLLQTRKRFGEGRKRALAAVARDGA
AARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLP
VHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAEN
AVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMCKLRNGERADRLRSYAS
>Mature_318_residues
AEATTNRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAEHLLQTRKRFGEGRKRALAAVARDGAA
ARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPV
HLVEQVNKLARIKREMHQNLGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAENA
VIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERDVMCKLRNGERADRLRSYAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=269, Percent_Identity=47.9553903345725, Blast_Score=264, Evalue=5e-72,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=39.1608391608392, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=29.0598290598291, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35847; Mature: 35716

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEATTNRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAEHLLQT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RKRFGEGRKRALAAVARDGAAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAEN
HCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERD
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
VMCKLRNGERADRLRSYAS
HHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
AEATTNRVDSDLDAQSPAADLVRVYLNGIGKTALLNAADEVELAKRIEAGLYAEHLLQT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RKRFGEGRKRALAAVARDGAAARRHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHH
RAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LGREATDEELAAESGIPIEKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAEN
HCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
AVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQIERD
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
VMCKLRNGERADRLRSYAS
HHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]