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The map label for this gene is ppdK [H]

Identifier: 221229932

GI number: 221229932

Start: 1123058

End: 1124863

Strand: Reverse

Name: ppdK [H]

Synonym: MLBr_00955

Alternate gene names: 221229932

Gene position: 1124863-1123058 (Counterclockwise)

Preceding gene: 221229934

Following gene: 221229930

Centisome position: 34.42

GC content: 59.36

Gene sequence:

>1806_bases
TTGCCCGGGCAGTCCCATAAAGAACCGCACAGGAGAGCAACATCAACACCGCGATCATTGAAGCAGGCAACGTCAGAATT
GCGTTGGGCACTCAGCCTAGCTGCACCGGAATCCCCAGCATTCATGCGACACCTACTGGGAAACGCTCATGACAAAGTGG
ACATCACGACATCGAGCGAACATGTCGCCGCGTTCACAGAACACGCCATAGTGTTGCTGAATGGTAATGCACACCAAACG
CGGGAACTCCTGGGCGGCAAGGGCTATAACATCAACGCGATGCGCCGACACCAACTGGCAGTACCCCCAGCGTTCTGCAT
CACCACCGAGGTCGGCGTTCGGTATTTGGCCGCTCCCACGCCGACGATGGAAGCGATCTGGAACGACGTGCTGGACCAGA
TGCGATGGCTGGAAGCCGAAACCTCACGCACATTTGGGTGGGGACCGCGGCCATTGCTGGTCAGCATTCGCTCCGGAGCC
GTCCAATCGATGCCCGGGATGATGGACACCATTCTAAACTTAGGCATAAATGATCCCGTCGAGCAGGCACTGGCAGCGGC
CAGCCCCTCGCCGATCCCCCAAACCTTTGCCCGCGACACCCGTCTGCGGTTCATACGCACGTATCGGCGCATAGTGGATC
CGTTAAATCCCCCACCCGCCAATACCTATGACCAATTACGCGCGGCCATAGAGTCAATTTTCTCCTCATGGAACTCTCCT
CGCGCGGTTGCTTACCGCACCCACTACGGTCTAGACGATCAGAGTGGTACAGCTGTGATTGTGCAAGCGATGGTGTTTGG
CAACCAAGGCGCCTACTCGGGAGCCGGAACGTTATTTTCCCGCAATCCGATAACCAGAGAGAGTAAACTGTTCGGCGAGT
GGTTGCCTGGCGGACAAGGCGACGATGTGGTGTCAGGATTGGTTGAAGTCCAGCCGATCACGTCCCTGCGTGACGAGCAA
CCTACTGTCTACGACGAGCTAGTGGACACCGCTGGCCGCTTGGAGCGACTGGGATCTGACGTGCAAGAGATCGAGTTCAC
CACCGAGGACAGCAAGTTATGGCTGCTGCAGACCCGGGTCGCGGAACGCTCGGCGCAGGTCGCGGTGCGCCTGGCGCTGC
AACTGTGGCAGGAGGGGCTCATCGACGATGCCGAGGCACTGCGCCGGGTGACCCCGGTGCATGTCGAGACCGTGTTGCTA
CCCGTACTGCAACCGGAAAACCATTTATCTGCAACACTTTTAGCCAAAGGCACGCCCACTTGCCCGGGTGTGATATCAGG
CAAGGCGTACACCGAAGTTGATGAAGCATTAGACGCTGCCGAGTCGGCCGAACAGGTTATCCTGGTGCGCGACCACACCA
GGCCCGACGACGTCCTTGGCATGTTGGCATCACAGGGGGTTGTCACCGAGGTCGGCGGTGCCGCCAGTCATGCGGCAGTG
GTCAGCCGCGAACTCAGCCGAGTGGCCGTAGTGGGCTGCGGCCAGGGAACCGCCGTATCGTTGGCTGGTAAGCAGATCAC
CGTTGACGGCGACAAAGGCGAGGTACGCCAAGGCAATCTGACACTGTCCGCGTGGTCGGAGAACGAAACGCCGGAGCTGC
GCGAGCTGGCCGACATAGCACGACGTATCAGCCCACTGCGCGTGCACATGACCGAGGACCACACGAGGCTGGATGACTCG
TCCGATACTGCGGTGCGCTTAGCCATAAACACTGGGCATGCCGACGTGGTGTCAGCCACCCCGCTAATCGTCATGATGAC
CACTCTGCGGCTGCAAGACTCAAAGGATTCAAGCGCCAAATCGTGA

Upstream 100 bases:

>100_bases
AGTGTCAGGCCCAGCACCAGCCGCAGGATCAGTAACCCGATGTCATAGGAAGTCATAGATTCAAACCTAGATGATTTAAA
CAAATCCTTGGCAGGTACCG

Downstream 100 bases:

>100_bases
CTGAACTGACAGTGCTGCAAGCAGTTCGGCTGAAGGGCCGGGTAAGTTCTACCGACTTGGCCGCAACTTTGTACGACGAC
CTTGTCGAGGTAACCAAAAC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MPGQSHKEPHRRATSTPRSLKQATSELRWALSLAAPESPAFMRHLLGNAHDKVDITTSSEHVAAFTEHAIVLLNGNAHQT
RELLGGKGYNINAMRRHQLAVPPAFCITTEVGVRYLAAPTPTMEAIWNDVLDQMRWLEAETSRTFGWGPRPLLVSIRSGA
VQSMPGMMDTILNLGINDPVEQALAAASPSPIPQTFARDTRLRFIRTYRRIVDPLNPPPANTYDQLRAAIESIFSSWNSP
RAVAYRTHYGLDDQSGTAVIVQAMVFGNQGAYSGAGTLFSRNPITRESKLFGEWLPGGQGDDVVSGLVEVQPITSLRDEQ
PTVYDELVDTAGRLERLGSDVQEIEFTTEDSKLWLLQTRVAERSAQVAVRLALQLWQEGLIDDAEALRRVTPVHVETVLL
PVLQPENHLSATLLAKGTPTCPGVISGKAYTEVDEALDAAESAEQVILVRDHTRPDDVLGMLASQGVVTEVGGAASHAAV
VSRELSRVAVVGCGQGTAVSLAGKQITVDGDKGEVRQGNLTLSAWSENETPELRELADIARRISPLRVHMTEDHTRLDDS
SDTAVRLAINTGHADVVSATPLIVMMTTLRLQDSKDSSAKS

Sequences:

>Translated_601_residues
MPGQSHKEPHRRATSTPRSLKQATSELRWALSLAAPESPAFMRHLLGNAHDKVDITTSSEHVAAFTEHAIVLLNGNAHQT
RELLGGKGYNINAMRRHQLAVPPAFCITTEVGVRYLAAPTPTMEAIWNDVLDQMRWLEAETSRTFGWGPRPLLVSIRSGA
VQSMPGMMDTILNLGINDPVEQALAAASPSPIPQTFARDTRLRFIRTYRRIVDPLNPPPANTYDQLRAAIESIFSSWNSP
RAVAYRTHYGLDDQSGTAVIVQAMVFGNQGAYSGAGTLFSRNPITRESKLFGEWLPGGQGDDVVSGLVEVQPITSLRDEQ
PTVYDELVDTAGRLERLGSDVQEIEFTTEDSKLWLLQTRVAERSAQVAVRLALQLWQEGLIDDAEALRRVTPVHVETVLL
PVLQPENHLSATLLAKGTPTCPGVISGKAYTEVDEALDAAESAEQVILVRDHTRPDDVLGMLASQGVVTEVGGAASHAAV
VSRELSRVAVVGCGQGTAVSLAGKQITVDGDKGEVRQGNLTLSAWSENETPELRELADIARRISPLRVHMTEDHTRLDDS
SDTAVRLAINTGHADVVSATPLIVMMTTLRLQDSKDSSAKS
>Mature_600_residues
PGQSHKEPHRRATSTPRSLKQATSELRWALSLAAPESPAFMRHLLGNAHDKVDITTSSEHVAAFTEHAIVLLNGNAHQTR
ELLGGKGYNINAMRRHQLAVPPAFCITTEVGVRYLAAPTPTMEAIWNDVLDQMRWLEAETSRTFGWGPRPLLVSIRSGAV
QSMPGMMDTILNLGINDPVEQALAAASPSPIPQTFARDTRLRFIRTYRRIVDPLNPPPANTYDQLRAAIESIFSSWNSPR
AVAYRTHYGLDDQSGTAVIVQAMVFGNQGAYSGAGTLFSRNPITRESKLFGEWLPGGQGDDVVSGLVEVQPITSLRDEQP
TVYDELVDTAGRLERLGSDVQEIEFTTEDSKLWLLQTRVAERSAQVAVRLALQLWQEGLIDDAEALRRVTPVHVETVLLP
VLQPENHLSATLLAKGTPTCPGVISGKAYTEVDEALDAAESAEQVILVRDHTRPDDVLGMLASQGVVTEVGGAASHAAVV
SRELSRVAVVGCGQGTAVSLAGKQITVDGDKGEVRQGNLTLSAWSENETPELRELADIARRISPLRVHMTEDHTRLDDSS
DTAVRLAINTGHADVVSATPLIVMMTTLRLQDSKDSSAKS

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 65312; Mature: 65181

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQSHKEPHRRATSTPRSLKQATSELRWALSLAAPESPAFMRHLLGNAHDKVDITTSSE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCEEEECCCH
HVAAFTEHAIVLLNGNAHQTRELLGGKGYNINAMRRHQLAVPPAFCITTEVGVRYLAAPT
HHHEEECCEEEEEECCHHHHHHHHCCCCCCCCHHHHCCCCCCCHHEEEHHHCCEEEECCC
PTMEAIWNDVLDQMRWLEAETSRTFGWGPRPLLVSIRSGAVQSMPGMMDTILNLGINDPV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHCCHHHHHHHHCCCCCHH
EQALAAASPSPIPQTFARDTRLRFIRTYRRIVDPLNPPPANTYDQLRAAIESIFSSWNSP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RAVAYRTHYGLDDQSGTAVIVQAMVFGNQGAYSGAGTLFSRNPITRESKLFGEWLPGGQG
CEEEEEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCCEECCCCCCHHHHHHHHCCCCCCC
DDVVSGLVEVQPITSLRDEQPTVYDELVDTAGRLERLGSDVQEIEFTTEDSKLWLLQTRV
CHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEEEEEHHHH
AERSAQVAVRLALQLWQEGLIDDAEALRRVTPVHVETVLLPVLQPENHLSATLLAKGTPT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHEEECCCCCCCCCEEEEECCCCC
CPGVISGKAYTEVDEALDAAESAEQVILVRDHTRPDDVLGMLASQGVVTEVGGAASHAAV
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHCCCCHHHHHH
VSRELSRVAVVGCGQGTAVSLAGKQITVDGDKGEVRQGNLTLSAWSENETPELRELADIA
HHHHHCEEEEEECCCCCEEEECCCEEEEECCCCCEEECCEEEEECCCCCCHHHHHHHHHH
RRISPLRVHMTEDHTRLDDSSDTAVRLAINTGHADVVSATPLIVMMTTLRLQDSKDSSAK
HHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHCCCCCCCCCC
S
C
>Mature Secondary Structure 
PGQSHKEPHRRATSTPRSLKQATSELRWALSLAAPESPAFMRHLLGNAHDKVDITTSSE
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCCEEEECCCH
HVAAFTEHAIVLLNGNAHQTRELLGGKGYNINAMRRHQLAVPPAFCITTEVGVRYLAAPT
HHHEEECCEEEEEECCHHHHHHHHCCCCCCCCHHHHCCCCCCCHHEEEHHHCCEEEECCC
PTMEAIWNDVLDQMRWLEAETSRTFGWGPRPLLVSIRSGAVQSMPGMMDTILNLGINDPV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHCCHHHHHHHHCCCCCHH
EQALAAASPSPIPQTFARDTRLRFIRTYRRIVDPLNPPPANTYDQLRAAIESIFSSWNSP
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RAVAYRTHYGLDDQSGTAVIVQAMVFGNQGAYSGAGTLFSRNPITRESKLFGEWLPGGQG
CEEEEEEECCCCCCCCCEEEEEEHHHCCCCCCCCCCCEECCCCCCHHHHHHHHCCCCCCC
DDVVSGLVEVQPITSLRDEQPTVYDELVDTAGRLERLGSDVQEIEFTTEDSKLWLLQTRV
CHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCEEEEEHHHH
AERSAQVAVRLALQLWQEGLIDDAEALRRVTPVHVETVLLPVLQPENHLSATLLAKGTPT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHEEECCCCCCCCCEEEEECCCCC
CPGVISGKAYTEVDEALDAAESAEQVILVRDHTRPDDVLGMLASQGVVTEVGGAASHAAV
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHHCCCCHHHHHH
VSRELSRVAVVGCGQGTAVSLAGKQITVDGDKGEVRQGNLTLSAWSENETPELRELADIA
HHHHHCEEEEEECCCCCEEEECCCEEEEECCCCCEEECCEEEEECCCCCCHHHHHHHHHH
RRISPLRVHMTEDHTRLDDSSDTAVRLAINTGHADVVSATPLIVMMTTLRLQDSKDSSAK
HHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]