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The map label for this gene is pimB [H]

Identifier: 221229885

GI number: 221229885

Start: 1052175

End: 1053329

Strand: Direct

Name: pimB [H]

Synonym: MLBr_00886

Alternate gene names: 221229885

Gene position: 1052175-1053329 (Clockwise)

Preceding gene: 221229884

Following gene: 221229887

Centisome position: 32.2

GC content: 63.46

Gene sequence:

>1155_bases
GTGAGCCGGGTCCTGCTGGTAACCAATGATTTTCCGCCCCGCCGTGGTGGCATCCAATCCTATCTGGGGGAGTTTGTAGA
TCGTCTGATCAACATCGGGTCGCATGACGTGATGGTGTACGCACCGCAATGGACCGGTGCCGATACCTTTGACAACGCTG
CCCATGATGGCGGCTATCAGGTAGTGCGCCATCCAGGCACGTTGATGCTGCCCGGCCCGGCGGTCGATGCCCGGATGCGC
CGGCTGATTGTTGACCATGGTATCGACACTGTGTGGTTCGGCTCGGCAGCGCCGCTGGCTCTGCTGGCGCCGTGTGCCCG
AAAGGCGGGAGCGACCCGGGTGTTGGCTAGCACACACGGCCACGAAGTGGGCTGGTCGATGCTGCCGGTTGCACGCTCGG
TGTTGCGTCGCATCGGTGACGTTACCGACGTTGTGACCTTTGTCAGCCGTTACACGCGATCGCGTGTAACGGCTGCCTTC
GGACCCAGGGCTGCGTTGGAATACCTCCCGCCAGGTGTGGACGCCGATCGATTCCGGCCCGATCCGGCGAGCCGAGCCGA
GCTACGTAACCGTTACCGGTTGGGTGAGAGGCCAACCGTTGTGTGCCTGGCGCGGTTGGTGCCGCGTAAAGGCCAGGACA
TGTTGATCAGGGCATTACCATCGATCCGGCAGCGGGCCGACGGGGCCGCGTTGATGATTGTTGGGGGCGGCCCGTATCTT
GGAACGCTGCGTAAGTTGGCCCAGGGGTGTGGGGTTGCCGACCACGTGATATTCGCCGGAGGCGTGGCGGCTGTCGAGGT
ACCCGCTCACTACACGCTGGCTGATGTATTTGCAATGCCATGCCGCACCCGCGGCGGAGGCATGGACGTCGAGGGTTTGG
GCATCGTGTTCCTGGAGGCCTCGGCTACCGGCGTGCCGGTAATCGCTGGAAAATCCGGCGGAGCTCCGGAAGCTGTGCAG
CACAACAAGACTGGACTAGTAGTTGACGGCCGGTCGGTGAATATGGTGGCTGACGCAGTCACCGAGTTACTGACCGATCG
TGACCGGGCCGCTGCTATGGGCGCAGCCGGACGGCACTGGGTAACCGCCGAGTGGCGTTGGGACACGCTGGCAGCTCGGT
TGGCGGATCTGCTGTGTGGCAACGACAGCCGCTAG

Upstream 100 bases:

>100_bases
TGCGGGTGGTGCCGATGAATTCGTCGGGTCCGATCTACGACGCCCGCCGCTACTAAACGCCTCGAACTGAGCTCACCTGC
GCCAGGCTAGCCTGGCGCAG

Downstream 100 bases:

>100_bases
TCCTTTTCGTAGATCGCCTCAATGTCGGAGGCGAACTTCTGGGCGACCACATTGCGCTTCACTTTCATCGTCGGCGTCAG
TTCGCCGGTCTGCTCGGTGA

Product: putative glycosyl transferase

Products: NA

Alternate protein names: Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-manT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSRVLLVTNDFPPRRGGIQSYLGEFVDRLINIGSHDVMVYAPQWTGADTFDNAAHDGGYQVVRHPGTLMLPGPAVDARMR
RLIVDHGIDTVWFGSAAPLALLAPCARKAGATRVLASTHGHEVGWSMLPVARSVLRRIGDVTDVVTFVSRYTRSRVTAAF
GPRAALEYLPPGVDADRFRPDPASRAELRNRYRLGERPTVVCLARLVPRKGQDMLIRALPSIRQRADGAALMIVGGGPYL
GTLRKLAQGCGVADHVIFAGGVAAVEVPAHYTLADVFAMPCRTRGGGMDVEGLGIVFLEASATGVPVIAGKSGGAPEAVQ
HNKTGLVVDGRSVNMVADAVTELLTDRDRAAAMGAAGRHWVTAEWRWDTLAARLADLLCGNDSR

Sequences:

>Translated_384_residues
MSRVLLVTNDFPPRRGGIQSYLGEFVDRLINIGSHDVMVYAPQWTGADTFDNAAHDGGYQVVRHPGTLMLPGPAVDARMR
RLIVDHGIDTVWFGSAAPLALLAPCARKAGATRVLASTHGHEVGWSMLPVARSVLRRIGDVTDVVTFVSRYTRSRVTAAF
GPRAALEYLPPGVDADRFRPDPASRAELRNRYRLGERPTVVCLARLVPRKGQDMLIRALPSIRQRADGAALMIVGGGPYL
GTLRKLAQGCGVADHVIFAGGVAAVEVPAHYTLADVFAMPCRTRGGGMDVEGLGIVFLEASATGVPVIAGKSGGAPEAVQ
HNKTGLVVDGRSVNMVADAVTELLTDRDRAAAMGAAGRHWVTAEWRWDTLAARLADLLCGNDSR
>Mature_383_residues
SRVLLVTNDFPPRRGGIQSYLGEFVDRLINIGSHDVMVYAPQWTGADTFDNAAHDGGYQVVRHPGTLMLPGPAVDARMRR
LIVDHGIDTVWFGSAAPLALLAPCARKAGATRVLASTHGHEVGWSMLPVARSVLRRIGDVTDVVTFVSRYTRSRVTAAFG
PRAALEYLPPGVDADRFRPDPASRAELRNRYRLGERPTVVCLARLVPRKGQDMLIRALPSIRQRADGAALMIVGGGPYLG
TLRKLAQGCGVADHVIFAGGVAAVEVPAHYTLADVFAMPCRTRGGGMDVEGLGIVFLEASATGVPVIAGKSGGAPEAVQH
NKTGLVVDGRSVNMVADAVTELLTDRDRAAAMGAAGRHWVTAEWRWDTLAARLADLLCGNDSR

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose resid

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=298, Percent_Identity=27.1812080536913, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 41054; Mature: 40922

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVLLVTNDFPPRRGGIQSYLGEFVDRLINIGSHDVMVYAPQWTGADTFDNAAHDGGYQ
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
VVRHPGTLMLPGPAVDARMRRLIVDHGIDTVWFGSAAPLALLAPCARKAGATRVLASTHG
EEECCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCC
HEVGWSMLPVARSVLRRIGDVTDVVTFVSRYTRSRVTAAFGPRAALEYLPPGVDADRFRP
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHCCCC
DPASRAELRNRYRLGERPTVVCLARLVPRKGQDMLIRALPSIRQRADGAALMIVGGGPYL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
GTLRKLAQGCGVADHVIFAGGVAAVEVPAHYTLADVFAMPCRTRGGGMDVEGLGIVFLEA
HHHHHHHHHCCHHHHHHEECCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
SATGVPVIAGKSGGAPEAVQHNKTGLVVDGRSVNMVADAVTELLTDRDRAAAMGAAGRHW
CCCCCEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCE
VTAEWRWDTLAARLADLLCGNDSR
EEEECCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRVLLVTNDFPPRRGGIQSYLGEFVDRLINIGSHDVMVYAPQWTGADTFDNAAHDGGYQ
CEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
VVRHPGTLMLPGPAVDARMRRLIVDHGIDTVWFGSAAPLALLAPCARKAGATRVLASTHG
EEECCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCEEEEECCCC
HEVGWSMLPVARSVLRRIGDVTDVVTFVSRYTRSRVTAAFGPRAALEYLPPGVDADRFRP
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHCCCC
DPASRAELRNRYRLGERPTVVCLARLVPRKGQDMLIRALPSIRQRADGAALMIVGGGPYL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
GTLRKLAQGCGVADHVIFAGGVAAVEVPAHYTLADVFAMPCRTRGGGMDVEGLGIVFLEA
HHHHHHHHHCCHHHHHHEECCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
SATGVPVIAGKSGGAPEAVQHNKTGLVVDGRSVNMVADAVTELLTDRDRAAAMGAAGRHW
CCCCCEEEECCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCE
VTAEWRWDTLAARLADLLCGNDSR
EEEECCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]