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The map label for this gene is uvrD2 [H]

Identifier: 221229741

GI number: 221229741

Start: 769127

End: 771280

Strand: Direct

Name: uvrD2 [H]

Synonym: MLBr_00637

Alternate gene names: 221229741

Gene position: 769127-771280 (Clockwise)

Preceding gene: 221229740

Following gene: 221229742

Centisome position: 23.53

GC content: 61.05

Gene sequence:

>2154_bases
ATGGATCCTATGGTAATGGTCGTAAATCCATTGACTGCTGGACTGGATGACGAGCAGCGCGAGGCCGTGCTGGCTCCACG
CGGACCTGTTTGTGTGCTCGCGGGAGCCGGAACGGGTAAAACCCGCACCATCACTCACCGCATTGCCCACCTTGTTGGTG
CTGGTCACGTCGCCACTGGGCAGGTGCTGGCGGTGACATTCACCCAGCGTGCAGCGGCGGAGATGCGGTCCCGGTTGAGG
GCGCTTGGCGCTGCCGTACAAGCCGTTTCGGATTTTGGCGTTGTGCGAGTCCTGACCTTTCATGCTGCCGCGCACCGCCA
GTTGCGATATTTCTGGCCACGGGTGATCGGTGACACTGGCTGGCAGCTGTTGGACAGCAAGTTTGCCACCGTTGCGCGGG
CGGCTAGTAGTGTCAGGCTGCATGCTGGCACTGATGACGTGTGTGACCTCGCCGGCGAGATTGAATGGGCTAAGGCCTCG
CTGATTGGGCCCGAAGAATACGTGGCCGCCGTGGCCGCTATCGGTAGAGACACTCCGCTGGACGCCGCGCAAATCGCGAG
TGTCTACGCTGCCTACGAAGCTCTTAAGGCCCGCGGCAATGGCGTCCCAGGGGTCACGCTGCTCGACTTCGATGATCTGC
TGCTGCACACCGCCGCCGCGATCGAGAACGACGCAGCGGTAGCCGAGGAGTTCCGGGACCGTTACCGTTGTTTTGTAGTC
GATGAGTACCAAGACGTCACCCCGCTACAGCAACGGGTGCTTTCGGCCTGGTTGGGTGATCGAGACGATCTGACTGTGGT
CGGCGACGCCAACCAGACCATCTACTCGTTCACTGGGGCTTCACCTTGTTTCCTGCTTGACTTCCCCCGACGGTTTCCCG
ATGCCACGGTGGTGCGGCTGGAGCGCGACTACCGGTCTACTCCGCAGGTGGTGTCGCTTGCAAATCAGGTGATCGCAGCG
GCTCATGGCCGGGTTGCGGACAGCAAATTTCAGCTGTCCGGCCAGCGTGAGCCGGGTCCGGCCGCGTCATTTCACGAGTA
TTCTGATGAGCCTGCTGAGGCCGCTGCCGTGGCAGCATCAATTGCTCGACTGATCGAATTCGGCACCTCGCCATCCGAGA
TCGCGGTGCTTTACCGGGTCAATGCGCAGTCCGAGGCGTACGAGGAGGCGTTGACCGAGGTGGGGATTGCCTATCAGGTC
CGCGGCGGCGAGGGATTTTTCAACCGCCAGGAGATCAAACAGGCGCTACTAGCCCTGCAGCGTAGTGCGGAGCGCAGCTC
CCAAACAGAACCGAACAGCCCATTGTCGGACGTGGTCCGCGGTGTTCTGGAGCCATTGGGGCTAACGGCTGAGAAACCGG
TCGGCAGCCGCGCCCGGGATCGTTGGGAGGCGCTGACCGCATTGGCCGAACTGGCCGACGACGAAGTGGCTCAGCATCCG
CGGCTGGATCTTCCGGGGTTGCTGGCCGAGCTGCGGTTGCGGGCCGATGCCCGGCACCCACCGGTGGTACAGGGCGTCAC
GTTGGCCTCATTGCATGCCGTTAAAGGACTTGAGTGGGATGCGGTGTTCTTAGTCGGATTGGCCGACGGAACACTACCCA
TCTCGCGTGCGTTGGCGCATGGTGCTGAAAGCGAGCCTGTCGAGGAAGAGCGTAGGCTGCTTTATGTTGGAATCACCAGG
GCCCGAGTGTATTTGGCACTTAGCTGGGCGTTATCGCGAACTCCGGGTGGACGTCAGAGCCGCAAGCCGTCGCGGTTCCT
CAACGACATCGCGCCACAGATGGGTCAAAATCCTGCGTCGAGCAGATCTCGGCGTAATCGGACCGCTACTTTGCGCTGCC
GTATTTGCAAAAACGACCTGACCACGCCTGCGGCCGTCATGCTGCAGCGGTGTCAGATATGTGCCGCAGACGTTGACGAA
GAATTGTTGCTGCAGCTCAAAGCGTGGCGGCTTAGCACTGCCAAGGAACAGAACGTGCCTGCTTACGTCGTTTTAACCGA
TAACACGTTGATTGCGATTGCTGAGTTGCTGCCCGCCGATGAGGCCGCGTTGATCGCTGTCCCGGGTATGAGTGTACGCA
AAATTGAGCAATACGGGTCGGACGTGCTGCAGTTGGTTCGCTGCCGTGCCGTCGCGGTTAGGACGCAGACCTGA

Upstream 100 bases:

>100_bases
ACATAGCTTTACGACTATGCGGGCTTGGTGAGCAGGACCTGACACCGGGCGAGGCAACGGGCTTGGCGATGTGCAAGGTT
TCTCGGTCTTGGCTGCCAAG

Downstream 100 bases:

>100_bases
TCGATCGTACCTAGTTAACTGATAGCAGACAAACGCGCAGATAAAAATAAGTTTCCACTGCTGGCTGTTACCGTTGTGCT
CAAATCCGCGCTTTGCTGGC

Product: putative ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MDPMVMVVNPLTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHRIAHLVGAGHVATGQVLAVTFTQRAAAEMRSRLR
ALGAAVQAVSDFGVVRVLTFHAAAHRQLRYFWPRVIGDTGWQLLDSKFATVARAASSVRLHAGTDDVCDLAGEIEWAKAS
LIGPEEYVAAVAAIGRDTPLDAAQIASVYAAYEALKARGNGVPGVTLLDFDDLLLHTAAAIENDAAVAEEFRDRYRCFVV
DEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPCFLLDFPRRFPDATVVRLERDYRSTPQVVSLANQVIAA
AHGRVADSKFQLSGQREPGPAASFHEYSDEPAEAAAVAASIARLIEFGTSPSEIAVLYRVNAQSEAYEEALTEVGIAYQV
RGGEGFFNRQEIKQALLALQRSAERSSQTEPNSPLSDVVRGVLEPLGLTAEKPVGSRARDRWEALTALAELADDEVAQHP
RLDLPGLLAELRLRADARHPPVVQGVTLASLHAVKGLEWDAVFLVGLADGTLPISRALAHGAESEPVEEERRLLYVGITR
ARVYLALSWALSRTPGGRQSRKPSRFLNDIAPQMGQNPASSRSRRNRTATLRCRICKNDLTTPAAVMLQRCQICAADVDE
ELLLQLKAWRLSTAKEQNVPAYVVLTDNTLIAIAELLPADEAALIAVPGMSVRKIEQYGSDVLQLVRCRAVAVRTQT

Sequences:

>Translated_717_residues
MDPMVMVVNPLTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHRIAHLVGAGHVATGQVLAVTFTQRAAAEMRSRLR
ALGAAVQAVSDFGVVRVLTFHAAAHRQLRYFWPRVIGDTGWQLLDSKFATVARAASSVRLHAGTDDVCDLAGEIEWAKAS
LIGPEEYVAAVAAIGRDTPLDAAQIASVYAAYEALKARGNGVPGVTLLDFDDLLLHTAAAIENDAAVAEEFRDRYRCFVV
DEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPCFLLDFPRRFPDATVVRLERDYRSTPQVVSLANQVIAA
AHGRVADSKFQLSGQREPGPAASFHEYSDEPAEAAAVAASIARLIEFGTSPSEIAVLYRVNAQSEAYEEALTEVGIAYQV
RGGEGFFNRQEIKQALLALQRSAERSSQTEPNSPLSDVVRGVLEPLGLTAEKPVGSRARDRWEALTALAELADDEVAQHP
RLDLPGLLAELRLRADARHPPVVQGVTLASLHAVKGLEWDAVFLVGLADGTLPISRALAHGAESEPVEEERRLLYVGITR
ARVYLALSWALSRTPGGRQSRKPSRFLNDIAPQMGQNPASSRSRRNRTATLRCRICKNDLTTPAAVMLQRCQICAADVDE
ELLLQLKAWRLSTAKEQNVPAYVVLTDNTLIAIAELLPADEAALIAVPGMSVRKIEQYGSDVLQLVRCRAVAVRTQT
>Mature_717_residues
MDPMVMVVNPLTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHRIAHLVGAGHVATGQVLAVTFTQRAAAEMRSRLR
ALGAAVQAVSDFGVVRVLTFHAAAHRQLRYFWPRVIGDTGWQLLDSKFATVARAASSVRLHAGTDDVCDLAGEIEWAKAS
LIGPEEYVAAVAAIGRDTPLDAAQIASVYAAYEALKARGNGVPGVTLLDFDDLLLHTAAAIENDAAVAEEFRDRYRCFVV
DEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPCFLLDFPRRFPDATVVRLERDYRSTPQVVSLANQVIAA
AHGRVADSKFQLSGQREPGPAASFHEYSDEPAEAAAVAASIARLIEFGTSPSEIAVLYRVNAQSEAYEEALTEVGIAYQV
RGGEGFFNRQEIKQALLALQRSAERSSQTEPNSPLSDVVRGVLEPLGLTAEKPVGSRARDRWEALTALAELADDEVAQHP
RLDLPGLLAELRLRADARHPPVVQGVTLASLHAVKGLEWDAVFLVGLADGTLPISRALAHGAESEPVEEERRLLYVGITR
ARVYLALSWALSRTPGGRQSRKPSRFLNDIAPQMGQNPASSRSRRNRTATLRCRICKNDLTTPAAVMLQRCQICAADVDE
ELLLQLKAWRLSTAKEQNVPAYVVLTDNTLIAIAELLPADEAALIAVPGMSVRKIEQYGSDVLQLVRCRAVAVRTQT

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=448, Percent_Identity=33.9285714285714, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI48994965, Length=414, Percent_Identity=33.8164251207729, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6322369, Length=449, Percent_Identity=25.6124721603563, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324477, Length=396, Percent_Identity=21.7171717171717, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77687; Mature: 77687

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPMVMVVNPLTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHRIAHLVGAGHVATG
CCCCEEEECCCCCCCCCHHCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
QVLAVTFTQRAAAEMRSRLRALGAAVQAVSDFGVVRVLTFHAAAHRQLRYFWPRVIGDTG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
WQLLDSKFATVARAASSVRLHAGTDDVCDLAGEIEWAKASLIGPEEYVAAVAAIGRDTPL
HHHHHHHHHHHHHHHHCEEEECCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCC
DAAQIASVYAAYEALKARGNGVPGVTLLDFDDLLLHTAAAIENDAAVAEEFRDRYRCFVV
CHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEE
DEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPCFLLDFPRRFPDATVVRL
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEEE
ERDYRSTPQVVSLANQVIAAAHGRVADSKFQLSGQREPGPAASFHEYSDEPAEAAAVAAS
EHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
IARLIEFGTSPSEIAVLYRVNAQSEAYEEALTEVGIAYQVRGGEGFFNRQEIKQALLALQ
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHH
RSAERSSQTEPNSPLSDVVRGVLEPLGLTAEKPVGSRARDRWEALTALAELADDEVAQHP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RLDLPGLLAELRLRADARHPPVVQGVTLASLHAVKGLEWDAVFLVGLADGTLPISRALAH
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHC
GAESEPVEEERRLLYVGITRARVYLALSWALSRTPGGRQSRKPSRFLNDIAPQMGQNPAS
CCCCCCCHHHCCEEEEEEHHHHEEHEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCH
SRSRRNRTATLRCRICKNDLTTPAAVMLQRCQICAADVDEELLLQLKAWRLSTAKEQNVP
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
AYVVLTDNTLIAIAELLPADEAALIAVPGMSVRKIEQYGSDVLQLVRCRAVAVRTQT
EEEEEECCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MDPMVMVVNPLTAGLDDEQREAVLAPRGPVCVLAGAGTGKTRTITHRIAHLVGAGHVATG
CCCCEEEECCCCCCCCCHHCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
QVLAVTFTQRAAAEMRSRLRALGAAVQAVSDFGVVRVLTFHAAAHRQLRYFWPRVIGDTG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
WQLLDSKFATVARAASSVRLHAGTDDVCDLAGEIEWAKASLIGPEEYVAAVAAIGRDTPL
HHHHHHHHHHHHHHHHCEEEECCCCHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCC
DAAQIASVYAAYEALKARGNGVPGVTLLDFDDLLLHTAAAIENDAAVAEEFRDRYRCFVV
CHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEE
DEYQDVTPLQQRVLSAWLGDRDDLTVVGDANQTIYSFTGASPCFLLDFPRRFPDATVVRL
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCCCCEEEEE
ERDYRSTPQVVSLANQVIAAAHGRVADSKFQLSGQREPGPAASFHEYSDEPAEAAAVAAS
EHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
IARLIEFGTSPSEIAVLYRVNAQSEAYEEALTEVGIAYQVRGGEGFFNRQEIKQALLALQ
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHH
RSAERSSQTEPNSPLSDVVRGVLEPLGLTAEKPVGSRARDRWEALTALAELADDEVAQHP
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RLDLPGLLAELRLRADARHPPVVQGVTLASLHAVKGLEWDAVFLVGLADGTLPISRALAH
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHC
GAESEPVEEERRLLYVGITRARVYLALSWALSRTPGGRQSRKPSRFLNDIAPQMGQNPAS
CCCCCCCHHHCCEEEEEEHHHHEEHEEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCH
SRSRRNRTATLRCRICKNDLTTPAAVMLQRCQICAADVDEELLLQLKAWRLSTAKEQNVP
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
AYVVLTDNTLIAIAELLPADEAALIAVPGMSVRKIEQYGSDVLQLVRCRAVAVRTQT
EEEEEECCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]