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The map label for this gene is fmt

Identifier: 221229682

GI number: 221229682

Start: 669012

End: 669968

Strand: Direct

Name: fmt

Synonym: MLBr_00552

Alternate gene names: 221229682

Gene position: 669012-669968 (Clockwise)

Preceding gene: 221229680

Following gene: 221229683

Centisome position: 20.47

GC content: 63.22

Gene sequence:

>957_bases
GTGCTGGTGCGTCTCGTGTTTGCCGGTACCCCTGAATCTGCGCTGCCCGCGCTGTGTCGTCTTATCGACTCGCCCCGTCA
TGACGTGATTGCGGTGTTGACCCGGCCCGACGCCGCATCTGGTCGGCGAGGCAAGCCAGAGCCGTCTCCTGTGGCCCGCG
AGGCGCTTGACCGTGGCATTCCGTTGCTGCGGCCAGCGCGGCCGAACTCGCCCGTGTTCGTTAGTGAACTATCGGAGTGG
GCCCCCGAGTGTTGTGTGGTGGTGGCCTACGGCGCACTGCTGGGCAGTCCGTTGCTCGCAGTGCCCCCGCGGGGCTGGGT
TAACTTGCACTTCTCGCTGCTGCCGGCTTGGCGTGGCGCGGCGCCGGTGCAAGCGGCGATCGCCGCCGGGGACACGATCA
CCGGAGCGACAACGTTCCAGATCGAGCCGAGCTTGGATTCTGGACCGGTTTACGGAGTGGTCACCGAGACGATCCAGCCG
ACTGACACCGCCGGCGATCTGCTTGAGCGACTGGCGGTTTCCGGGGCTACACTGCTGTCGAGCACACTGGACGGCATCGC
CGACGCTATATTGACGCCGCGACAGCAACCTGTCGACGGGGTCAGCTTCGCGCCGAAAATAACTGTTGAGCAGGCCCGGG
TGTGTTGGGATCTTCCGGCACCGGTTGTTGAGCGTCGTATTCGGGCGGTCACGCCCAATCCGGGTGCATGGACACTTGTC
GGTAAGTTACGGGTCAAACTTGGACCGGTGCGCTTTGATTCTGGCGCTGTTGAGGTGCCGAGGTTGTTAAAGCCCTTGCT
GCCCGGTGGTATTCATGTAGACCACAAAAGCGTCTGGATCGGCACTGGCTCAGATCCGGTGCGGTTGAGCAAAGTGCAAC
CGCAGGGAAAGAAATTCATGAACGCTGTAGACTGGGCGCATGGCGCCCGACTGGATCCCGCAGCGCGGGCATCATGA

Upstream 100 bases:

>100_bases
TGTATATAACATCTGTATATGTACGTATATCGCTTATGTGGCTTACTTCACATGTGCACAGGTTGCGGGATAGCAAGGAC
TTGGAGACCGTTTTTAGACT

Downstream 100 bases:

>100_bases
CTTCGGTTCAGGACAAATCACACCGAATTTAGCTAGATTGTAAACTAATCCGGCAGCAAAACCTAGGACGGTGACCCCGC
AGCCGACCTCTGGTCCTGGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLVRLVFAGTPESALPALCRLIDSPRHDVIAVLTRPDAASGRRGKPEPSPVAREALDRGIPLLRPARPNSPVFVSELSEW
APECCVVVAYGALLGSPLLAVPPRGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPVYGVVTETIQP
TDTAGDLLERLAVSGATLLSSTLDGIADAILTPRQQPVDGVSFAPKITVEQARVCWDLPAPVVERRIRAVTPNPGAWTLV
GKLRVKLGPVRFDSGAVEVPRLLKPLLPGGIHVDHKSVWIGTGSDPVRLSKVQPQGKKFMNAVDWAHGARLDPAARAS

Sequences:

>Translated_318_residues
MLVRLVFAGTPESALPALCRLIDSPRHDVIAVLTRPDAASGRRGKPEPSPVAREALDRGIPLLRPARPNSPVFVSELSEW
APECCVVVAYGALLGSPLLAVPPRGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPVYGVVTETIQP
TDTAGDLLERLAVSGATLLSSTLDGIADAILTPRQQPVDGVSFAPKITVEQARVCWDLPAPVVERRIRAVTPNPGAWTLV
GKLRVKLGPVRFDSGAVEVPRLLKPLLPGGIHVDHKSVWIGTGSDPVRLSKVQPQGKKFMNAVDWAHGARLDPAARAS
>Mature_318_residues
MLVRLVFAGTPESALPALCRLIDSPRHDVIAVLTRPDAASGRRGKPEPSPVAREALDRGIPLLRPARPNSPVFVSELSEW
APECCVVVAYGALLGSPLLAVPPRGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPVYGVVTETIQP
TDTAGDLLERLAVSGATLLSSTLDGIADAILTPRQQPVDGVSFAPKITVEQARVCWDLPAPVVERRIRAVTPNPGAWTLV
GKLRVKLGPVRFDSGAVEVPRLLKPLLPGGIHVDHKSVWIGTGSDPVRLSKVQPQGKKFMNAVDWAHGARLDPAARAS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=328, Percent_Identity=28.6585365853659, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI21614513, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI238814322, Length=235, Percent_Identity=26.8085106382979, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789683, Length=309, Percent_Identity=35.9223300970874, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788589, Length=227, Percent_Identity=30.8370044052863, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI133930964, Length=264, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550868, Length=313, Percent_Identity=29.073482428115, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571984, Length=246, Percent_Identity=32.520325203252, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_MYCLB (B8ZUM6)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503098.1
- ProteinModelPortal:   B8ZUM6
- SMR:   B8ZUM6
- EnsemblBacteria:   EBMYCT00000086675
- GeneID:   7326928
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015792
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33677; Mature: 33677

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVRLVFAGTPESALPALCRLIDSPRHDVIAVLTRPDAASGRRGKPEPSPVAREALDRGI
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PLLRPARPNSPVFVSELSEWAPECCVVVAYGALLGSPLLAVPPRGWVNLHFSLLPAWRGA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCC
APVQAAIAAGDTITGATTFQIEPSLDSGPVYGVVTETIQPTDTAGDLLERLAVSGATLLS
CCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHH
STLDGIADAILTPRQQPVDGVSFAPKITVEQARVCWDLPAPVVERRIRAVTPNPGAWTLV
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHEECCCHHHHHHHHEECCCCCCCEEEE
GKLRVKLGPVRFDSGAVEVPRLLKPLLPGGIHVDHKSVWIGTGSDPVRLSKVQPQGKKFM
EEEEEEECCEEECCCCCHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEECCCHHHHHH
NAVDWAHGARLDPAARAS
HHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MLVRLVFAGTPESALPALCRLIDSPRHDVIAVLTRPDAASGRRGKPEPSPVAREALDRGI
CEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PLLRPARPNSPVFVSELSEWAPECCVVVAYGALLGSPLLAVPPRGWVNLHFSLLPAWRGA
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCC
APVQAAIAAGDTITGATTFQIEPSLDSGPVYGVVTETIQPTDTAGDLLERLAVSGATLLS
CCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHH
STLDGIADAILTPRQQPVDGVSFAPKITVEQARVCWDLPAPVVERRIRAVTPNPGAWTLV
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHEECCCHHHHHHHHEECCCCCCCEEEE
GKLRVKLGPVRFDSGAVEVPRLLKPLLPGGIHVDHKSVWIGTGSDPVRLSKVQPQGKKFM
EEEEEEECCEEECCCCCHHHHHHHHHCCCCEEECCCEEEEECCCCCEEEEECCCHHHHHH
NAVDWAHGARLDPAARAS
HHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA