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The map label for this gene is ubiE [C]

Identifier: 221229681

GI number: 221229681

Start: 668007

End: 668831

Strand: Reverse

Name: ubiE [C]

Synonym: MLBr_00551

Alternate gene names: 221229681

Gene position: 668831-668007 (Counterclockwise)

Preceding gene: 221229685

Following gene: 221229666

Centisome position: 20.47

GC content: 58.79

Gene sequence:

>825_bases
ATGAACCTCTACACCCCCATCCACGAGGATCAGTCGCTTGCGGCTATTCATCGGGCGATGTGGGCACTGGGCGACTACGC
CCTGATGGCCGAAGAGGTGATGGCCCCACTCGGACCAATCCTGGTCACCGCCACGGGCATTCGACCGGGCATGCAAGTAC
TCGACGTCGCTGCCGGCTCTGGCAACATCTCGCTTCCCGCTGCCAAGACGGGCGCCACCATTGTCTCCACCGACCTGACG
CCGGAGCTGTTGCAGCGTTCCCAAGCCAGGGCCACTGAGCTCGGATTGACGCTCGAATACCGAGAAGCCGATGCACAAGC
CCTGCCGTTTGGTGACGGAGAGTTCGATGTCGTGATGTCAGCAATCGGCGTGATGTTCGCGCCAGACCATCGACGCGCTG
CCAGCGAGCTAGTTCGAGTTTGCCGACCCAACGGGACAATCGGTGTGATCAGCTGGACACAAGAGGGATTCTTCGGCCGA
ATGCTGGCTACCATCAGGCCATACCGGCCAAGCCTCTCTGCAGCTTTACCCCCGGCGGCACTATGGGGACGCGAGGCCTA
CATCCGAGGGCTACTAGACGACACCGTCACCAACGTCAAAACAGAACGCAGACAGCTCGAGGTAAAGCGGTTCAACTCCG
CCGAGGGCGTCCACAATTACTTCAAGAACCATTACGGCCCGACGATCGAGGCCTACACAAACATCGGCGACAACTCAGTA
CTCGCCGCTGAACTCGATGCGCAACTCACCGAACTCGCACAAAAATACCTAACCAACAGCACTATGGGTTGGGAATATCT
ATTGCTCACCGCGCAAAAACACTGA

Upstream 100 bases:

>100_bases
ATATACAGATGTTATATACATTTATCATATGTTATGGACATTTACCATTGCTAGTGCTTATTATTTCTAGCGAGCACGAT
TGCACTTGGGAGGAGACCGA

Downstream 100 bases:

>100_bases
AGACCGGCCGCCTCGAGCTCGGCCCCCCCCTCCCCCAATGCCGAGCTCGAGGCGCTGCCACAAGGAAGAAAGAATTAGCC
ACGCGCTATTAACAGCTAAC

Product: putative methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNLYTPIHEDQSLAAIHRAMWALGDYALMAEEVMAPLGPILVTATGIRPGMQVLDVAAGSGNISLPAAKTGATIVSTDLT
PELLQRSQARATELGLTLEYREADAQALPFGDGEFDVVMSAIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGR
MLATIRPYRPSLSAALPPAALWGREAYIRGLLDDTVTNVKTERRQLEVKRFNSAEGVHNYFKNHYGPTIEAYTNIGDNSV
LAAELDAQLTELAQKYLTNSTMGWEYLLLTAQKH

Sequences:

>Translated_274_residues
MNLYTPIHEDQSLAAIHRAMWALGDYALMAEEVMAPLGPILVTATGIRPGMQVLDVAAGSGNISLPAAKTGATIVSTDLT
PELLQRSQARATELGLTLEYREADAQALPFGDGEFDVVMSAIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGR
MLATIRPYRPSLSAALPPAALWGREAYIRGLLDDTVTNVKTERRQLEVKRFNSAEGVHNYFKNHYGPTIEAYTNIGDNSV
LAAELDAQLTELAQKYLTNSTMGWEYLLLTAQKH
>Mature_274_residues
MNLYTPIHEDQSLAAIHRAMWALGDYALMAEEVMAPLGPILVTATGIRPGMQVLDVAAGSGNISLPAAKTGATIVSTDLT
PELLQRSQARATELGLTLEYREADAQALPFGDGEFDVVMSAIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGR
MLATIRPYRPSLSAALPPAALWGREAYIRGLLDDTVTNVKTERRQLEVKRFNSAEGVHNYFKNHYGPTIEAYTNIGDNSV
LAAELDAQLTELAQKYLTNSTMGWEYLLLTAQKH

Specific function: Methyltransferase Required For The Conversion Of Dimethylmenaquinone (Dmkh2) To Menaquinone (Mkh2) And The Conversion Of 2-Polyprenyl-6-Methoxy-1,4-Benzoquinol (Ddmqh2) To 2-Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol (Dmqh2). [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.tuberculosis Rv1403c [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=118, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29950; Mature: 29950

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYTPIHEDQSLAAIHRAMWALGDYALMAEEVMAPLGPILVTATGIRPGMQVLDVAAGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEECCC
GNISLPAAKTGATIVSTDLTPELLQRSQARATELGLTLEYREADAQALPFGDGEFDVVMS
CCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHH
AIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGRMLATIRPYRPSLSAALPPAA
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHH
LWGREAYIRGLLDDTVTNVKTERRQLEVKRFNSAEGVHNYFKNHYGPTIEAYTNIGDNSV
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCE
LAAELDAQLTELAQKYLTNSTMGWEYLLLTAQKH
EEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MNLYTPIHEDQSLAAIHRAMWALGDYALMAEEVMAPLGPILVTATGIRPGMQVLDVAAGS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEECCC
GNISLPAAKTGATIVSTDLTPELLQRSQARATELGLTLEYREADAQALPFGDGEFDVVMS
CCEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHH
AIGVMFAPDHRRAASELVRVCRPNGTIGVISWTQEGFFGRMLATIRPYRPSLSAALPPAA
HHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCHH
LWGREAYIRGLLDDTVTNVKTERRQLEVKRFNSAEGVHNYFKNHYGPTIEAYTNIGDNSV
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCE
LAAELDAQLTELAQKYLTNSTMGWEYLLLTAQKH
EEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]