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The map label for this gene is aspS

Identifier: 221229649

GI number: 221229649

Start: 606848

End: 608617

Strand: Direct

Name: aspS

Synonym: MLBr_00501

Alternate gene names: 221229649

Gene position: 606848-608617 (Clockwise)

Preceding gene: 221229648

Following gene: 221229650

Centisome position: 18.57

GC content: 61.81

Gene sequence:

>1770_bases
GTGCTGCGTAGCCACGGCGCTGGTGTGTTACGGAAAAGCGATGCCGGGCAGCAGGTAACATTAGCTGGCTGGGTGTCTCG
CCGTCGAGACCACGGCGGCGTCATCTTCATTGATCTGCGCGACTCCTCGGGCATCACACAGGCGGTGTTTCGCGAGCCTG
ACGTGTTGGCCCAGGCGCATCGGCTGCGCGCTGAGTTCTGCATCGCGGTTTCCGGCGTAGTCGAGATTCGCCCCGAGGGT
AACGCCAATGCCGAGATTCCCACCGGCGATATCGAGGTCAACGCCACTTCGCTGACCGTGCTCGGTGAGAGTGCGCCGCT
ACCATTCCAACTCGATGAGCCTGCGGGTGAGGAATTGCGGTTGAAGTACCGTTACCTCGACCTGCGCCGCGACGGTCCTG
GATCGGCAATTCGGTTGCGTTCCAAGGTGAATGCCACTGCTCGTGCAGTGCTGGCGCGCCACGATTTCGTTGAGATCGAA
ACCCCGACGATCACCCGCTCAACCCCGGAGGGGGCGCGCGACTTTTTAGTTCCGGCCCGGTTGCGGCCGGGCACGTTCTA
TGCTCTGCCGCAGAGCCCGCAATTGTTCAAACAGTTGCTGATGGTGGCCGGGATGGAGCGTTATTACCAGATTGCCCACT
GCTACCGCGACGAGGATTTCCGTGCCGACCGCCAGCCCGAATTCACTCAGCTCGACATGGAGATGAGCTTCGTCGATGCC
GAGGATGTCATCGCGATCTCCGAGGAAATCCTAACCGAGCTCTGGATGCTGATAGGCTACCACATTCCGGCGCCGATCCC
GCGGATCAGCTATGCGGATGCTATGCGACGGTTCGGCTCTGACAAGCCCGACTTGCGGTTCGGGCTGGAGCTTGTTGAAT
GCACAGAGTTCTTCTGCGACACAACGTTCCGTGTCTTCCAGGCACCGTATGTCGGTGCGGTAGTGATGCCCGGCGGTGCT
GCACAGCCGCGGCGCACACTGGACGAGTGGCAGGACTGGGCCAAACAGCGCGGGCATCGCGGCCTGGCCTACGTGCTGGT
GACCGATGACGGTACGCTCGGCGGACCGGTGGCCAAGAACTTCAGCGATGCCGAACGCTCTCGCCTGGCCTCCCACGTAG
GCGCCGAACCCGGGGACTGCATCTTCTTTTCAGCGGGCCCGGCGAAGTCGTCGCGGGCACTGCTGGGGGCCGCCCGCGGT
GAGATCGCCAACCGGCTGGGTTTGATCGACCCCGAGGCCTGGGCGTTCGTCTGGGTAGTCGACCCGCCGCTTTTCGAACG
CGCCGACGAGGCCACCAAGGTCGGCGAAATGGCAGTGGGCTCTGGTGCGTGGACTGCGGTGCATCATGCCTTCACATCGC
CGAAACCGGAATTCGAGGACAGCATAGAGTCCGATCCCGGCAGCGTGTTGGCCGATGCCTACGACATCGTTTGTAACGGC
CACGAGATTGGTAGCGGCTCGGTTCGTATTAACCGCCGTGATATCCAGGAACGGGTGTTTGCGGTTATGGGATTGGAAAA
GGCTGAGGCGGAGGAGAAATTCGGGTTCCTTTTGGAGGCTTTTACGTTTGGTGCGCCTCCGCACGGCGGTATCGCCTTTG
GCTGGGATCGGACCAATGCCTTGCTGGCCGGGATGGAGTCGATACGTGAGGTGATTGCGTTCCCCAAGACCGGCGGTGGT
GTCGACCCGCTCACTGATGCGCCGGCTTCGATAACCGCTCAGCAGCGCAAGGAATCCGGAATAGACACCAAGCCCAAAGA
GGTTGAGTAG

Upstream 100 bases:

>100_bases
AATGAAATCTTGGTTGTGGGGTTGGTAGCGATACGGCTGAAAGTTGCACCACCCTCTAGACTGGGCAGTCGTTAGTTGTC
AACTAAAACGGGGAGTGTTT

Downstream 100 bases:

>100_bases
GCATGACAGAATAGTTTCCGGACGCTGAAACGGTAAAGGAGTTCAACAAAACCATCGTCGACGAGTTCCAGGACAACGGC
GGCAAGGTCGGTGGGCAATT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MLRSHGAGVLRKSDAGQQVTLAGWVSRRRDHGGVIFIDLRDSSGITQAVFREPDVLAQAHRLRAEFCIAVSGVVEIRPEG
NANAEIPTGDIEVNATSLTVLGESAPLPFQLDEPAGEELRLKYRYLDLRRDGPGSAIRLRSKVNATARAVLARHDFVEIE
TPTITRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLMVAGMERYYQIAHCYRDEDFRADRQPEFTQLDMEMSFVDA
EDVIAISEEILTELWMLIGYHIPAPIPRISYADAMRRFGSDKPDLRFGLELVECTEFFCDTTFRVFQAPYVGAVVMPGGA
AQPRRTLDEWQDWAKQRGHRGLAYVLVTDDGTLGGPVAKNFSDAERSRLASHVGAEPGDCIFFSAGPAKSSRALLGAARG
EIANRLGLIDPEAWAFVWVVDPPLFERADEATKVGEMAVGSGAWTAVHHAFTSPKPEFEDSIESDPGSVLADAYDIVCNG
HEIGSGSVRINRRDIQERVFAVMGLEKAEAEEKFGFLLEAFTFGAPPHGGIAFGWDRTNALLAGMESIREVIAFPKTGGG
VDPLTDAPASITAQQRKESGIDTKPKEVE

Sequences:

>Translated_589_residues
MLRSHGAGVLRKSDAGQQVTLAGWVSRRRDHGGVIFIDLRDSSGITQAVFREPDVLAQAHRLRAEFCIAVSGVVEIRPEG
NANAEIPTGDIEVNATSLTVLGESAPLPFQLDEPAGEELRLKYRYLDLRRDGPGSAIRLRSKVNATARAVLARHDFVEIE
TPTITRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLMVAGMERYYQIAHCYRDEDFRADRQPEFTQLDMEMSFVDA
EDVIAISEEILTELWMLIGYHIPAPIPRISYADAMRRFGSDKPDLRFGLELVECTEFFCDTTFRVFQAPYVGAVVMPGGA
AQPRRTLDEWQDWAKQRGHRGLAYVLVTDDGTLGGPVAKNFSDAERSRLASHVGAEPGDCIFFSAGPAKSSRALLGAARG
EIANRLGLIDPEAWAFVWVVDPPLFERADEATKVGEMAVGSGAWTAVHHAFTSPKPEFEDSIESDPGSVLADAYDIVCNG
HEIGSGSVRINRRDIQERVFAVMGLEKAEAEEKFGFLLEAFTFGAPPHGGIAFGWDRTNALLAGMESIREVIAFPKTGGG
VDPLTDAPASITAQQRKESGIDTKPKEVE
>Mature_589_residues
MLRSHGAGVLRKSDAGQQVTLAGWVSRRRDHGGVIFIDLRDSSGITQAVFREPDVLAQAHRLRAEFCIAVSGVVEIRPEG
NANAEIPTGDIEVNATSLTVLGESAPLPFQLDEPAGEELRLKYRYLDLRRDGPGSAIRLRSKVNATARAVLARHDFVEIE
TPTITRSTPEGARDFLVPARLRPGTFYALPQSPQLFKQLLMVAGMERYYQIAHCYRDEDFRADRQPEFTQLDMEMSFVDA
EDVIAISEEILTELWMLIGYHIPAPIPRISYADAMRRFGSDKPDLRFGLELVECTEFFCDTTFRVFQAPYVGAVVMPGGA
AQPRRTLDEWQDWAKQRGHRGLAYVLVTDDGTLGGPVAKNFSDAERSRLASHVGAEPGDCIFFSAGPAKSSRALLGAARG
EIANRLGLIDPEAWAFVWVVDPPLFERADEATKVGEMAVGSGAWTAVHHAFTSPKPEFEDSIESDPGSVLADAYDIVCNG
HEIGSGSVRINRRDIQERVFAVMGLEKAEAEEKFGFLLEAFTFGAPPHGGIAFGWDRTNALLAGMESIREVIAFPKTGGG
VDPLTDAPASITAQQRKESGIDTKPKEVE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=37.5, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI45439306, Length=239, Percent_Identity=28.8702928870293, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194272210, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI5031815, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI4758762, Length=281, Percent_Identity=24.9110320284698, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI226491502, Length=268, Percent_Identity=22.3880597014925, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788173, Length=581, Percent_Identity=45.7831325301205, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1790571, Length=303, Percent_Identity=25.4125412541254, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=268, Percent_Identity=26.4925373134328, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=34.6534653465347, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17551876, Length=271, Percent_Identity=28.0442804428044, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=144, Percent_Identity=30.5555555555556, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=144, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71984122, Length=297, Percent_Identity=24.9158249158249, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=624, Percent_Identity=31.7307692307692, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321807, Length=317, Percent_Identity=25.2365930599369, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=275, Percent_Identity=26.5454545454545, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=36.6056572379368, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI17136276, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24640851, Length=247, Percent_Identity=27.5303643724696, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640849, Length=247, Percent_Identity=27.5303643724696, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921528, Length=284, Percent_Identity=25.3521126760563, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_MYCLB (B8ZUJ3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503065.1
- ProteinModelPortal:   B8ZUJ3
- SMR:   B8ZUJ3
- EnsemblBacteria:   EBMYCT00000086221
- GeneID:   7325680
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017004
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 64632; Mature: 64632

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSHGAGVLRKSDAGQQVTLAGWVSRRRDHGGVIFIDLRDSSGITQAVFREPDVLAQAH
CCCCCCCCCEEECCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHH
RLRAEFCIAVSGVVEIRPEGNANAEIPTGDIEVNATSLTVLGESAPLPFQLDEPAGEELR
HHHHHHEEEEECEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCCCEE
LKYRYLDLRRDGPGSAIRLRSKVNATARAVLARHDFVEIETPTITRSTPEGARDFLVPAR
EEEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHCCEEEECCCEEECCCCCCCHHCCCCCC
LRPGTFYALPQSPQLFKQLLMVAGMERYYQIAHCYRDEDFRADRQPEFTQLDMEMSFVDA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECH
EDVIAISEEILTELWMLIGYHIPAPIPRISYADAMRRFGSDKPDLRFGLELVECTEFFCD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TTFRVFQAPYVGAVVMPGGAAQPRRTLDEWQDWAKQRGHRGLAYVLVTDDGTLGGPVAKN
CHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCC
FSDAERSRLASHVGAEPGDCIFFSAGPAKSSRALLGAARGEIANRLGLIDPEAWAFVWVV
CCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DPPLFERADEATKVGEMAVGSGAWTAVHHAFTSPKPEFEDSIESDPGSVLADAYDIVCNG
CCHHHHHCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHEEEEECC
HEIGSGSVRINRRDIQERVFAVMGLEKAEAEEKFGFLLEAFTFGAPPHGGIAFGWDRTNA
EEECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHH
LLAGMESIREVIAFPKTGGGVDPLTDAPASITAQQRKESGIDTKPKEVE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLRSHGAGVLRKSDAGQQVTLAGWVSRRRDHGGVIFIDLRDSSGITQAVFREPDVLAQAH
CCCCCCCCCEEECCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHH
RLRAEFCIAVSGVVEIRPEGNANAEIPTGDIEVNATSLTVLGESAPLPFQLDEPAGEELR
HHHHHHEEEEECEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCCCEE
LKYRYLDLRRDGPGSAIRLRSKVNATARAVLARHDFVEIETPTITRSTPEGARDFLVPAR
EEEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHCCEEEECCCEEECCCCCCCHHCCCCCC
LRPGTFYALPQSPQLFKQLLMVAGMERYYQIAHCYRDEDFRADRQPEFTQLDMEMSFVDA
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECH
EDVIAISEEILTELWMLIGYHIPAPIPRISYADAMRRFGSDKPDLRFGLELVECTEFFCD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
TTFRVFQAPYVGAVVMPGGAAQPRRTLDEWQDWAKQRGHRGLAYVLVTDDGTLGGPVAKN
CHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCC
FSDAERSRLASHVGAEPGDCIFFSAGPAKSSRALLGAARGEIANRLGLIDPEAWAFVWVV
CCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DPPLFERADEATKVGEMAVGSGAWTAVHHAFTSPKPEFEDSIESDPGSVLADAYDIVCNG
CCHHHHHCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHEEEEECC
HEIGSGSVRINRRDIQERVFAVMGLEKAEAEEKFGFLLEAFTFGAPPHGGIAFGWDRTNA
EEECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHH
LLAGMESIREVIAFPKTGGGVDPLTDAPASITAQQRKESGIDTKPKEVE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA