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The map label for this gene is hisS

Identifier: 221229648

GI number: 221229648

Start: 599749

End: 601032

Strand: Direct

Name: hisS

Synonym: MLBr_00494

Alternate gene names: 221229648

Gene position: 599749-601032 (Clockwise)

Preceding gene: 221229647

Following gene: 221229649

Centisome position: 18.35

GC content: 60.2

Gene sequence:

>1284_bases
GTGACGGAGTCATGCACTGTGTTTTCGTTTTCTGGGCCCAAGGGGATACCGGATTATTTTCCTCCGGACTCGGCGCAATT
TGTCGCGGTACGTGATGGTTTGCTCACTGCCGCCCGCAGGGCCGGTTACGGCGATATCGAGTTACCTGTTTTCGAGGACA
CCGCGTTGTTCGCTCGCGGCGTTGGTGAGTCCACTGACGTGGTAGCCAAGGAGATGTATACGTTTGCTGACCGTGGTGAT
CGCTCGGTTACGCTGCGTCCCGAGGGCACCGCTGGGGTGGTGCGTGCGGTGATTGAACACGGTCTGGACCGTGGAGCGTT
GCCGGTGAAGCTGTGCTACGCGGGCCCGTTTTTCCGATACGAGCGTCCCCAGGCCGGCCGATGTCGCCAACTGCAGCAGG
TCGGCGTTGAAGCAATCGGTGTCGACGACCCGGCCCTAGACGCCGAGGTGATCACCATCGCCGACGCTGGCTTCCGCTCC
CTGGGTCTGGACGGGTTCCAGTTGGAAATCACTTCGCTGGGCGATGGTACTTGTCGCCCGCAATATCGAAAACTGTTGCA
GGAGTTTCTCTTGCAGCTCGACCTTGACGAAGATACCCGCCGACGCGCCGAGCTTAATCCGCTGCGGGTGCTTGATGACA
AACGACCCCAGGTGCAAGCGATGACAGCCGCAGCTCCGGTGTTGCTAGACCATTTGTCCGATGGCGCCAAGCAGCACTTC
GATACGGTGCTGGCTCACCTGGACGCTCTGCGGGTGCCATATGTCATCAACCCGCGGATGGTGCGAGGGCTGGATTACTA
CACAAAAACAACCTTCGAGTTCGTCCACCCCGGCTTAGGTGCGCAATCCGGCATCGGCGGCGGTGGACGTTATGATGGTC
TAATGCGTCAGCTCGGCGGGCAGGACTTGTCGGGCATCGGATTCGGACTCGGGGTGGACCGGACCTTGCTGGCCCTACAT
GCCGAAGGCAAGACCGTGGGGGAAACCACACGCTGCGACGTATTCGGTGTGTCGCTGGGTGAAGCTGCTAAACTCAAGGT
GGCGATGTTGGCCGGGCAACTGCGTGCTGCCGGCGTCCGCGTCGACCTGATCTACGGGGATCGCGGGATCAGGGGAGCGA
TGCGTGCGGCGGGCCGTTCCGGTGCTCGCATCGCGTTGATAGTAGATGATTGCGCCATTAAGGCCGACGGTGTCGGTGTG
AGGGATCTAGCCACCGGTGAACAGATTTCGGTAGCTGTAGATTCTGTTGTCGCCGAAGTAATTTCGAGAATAGCTCCGAG
TTAG

Upstream 100 bases:

>100_bases
GGTACTCGACGACAAGACCGTGGTACTACCCGGGCATGGCAACTCCACCACCATCGGTGCCGAGCGTCGTTTCAATCCGT
TCCTCGAGGGCCTGTAGCTT

Downstream 100 bases:

>100_bases
CTCGGCTGACTTCGTCAGACGAAATTTGTCGTTCGTCTGAACCAGGGGTCGGCGTTATTGACCATACGGTTATTGACGTG
ATCGCAGAAGCCGGCTCGCG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTESCTVFSFSGPKGIPDYFPPDSAQFVAVRDGLLTAARRAGYGDIELPVFEDTALFARGVGESTDVVAKEMYTFADRGD
RSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRS
LGLDGFQLEITSLGDGTCRPQYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQAMTAAAPVLLDHLSDGAKQHF
DTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLGAQSGIGGGGRYDGLMRQLGGQDLSGIGFGLGVDRTLLALH
AEGKTVGETTRCDVFGVSLGEAAKLKVAMLAGQLRAAGVRVDLIYGDRGIRGAMRAAGRSGARIALIVDDCAIKADGVGV
RDLATGEQISVAVDSVVAEVISRIAPS

Sequences:

>Translated_427_residues
MTESCTVFSFSGPKGIPDYFPPDSAQFVAVRDGLLTAARRAGYGDIELPVFEDTALFARGVGESTDVVAKEMYTFADRGD
RSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRS
LGLDGFQLEITSLGDGTCRPQYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQAMTAAAPVLLDHLSDGAKQHF
DTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLGAQSGIGGGGRYDGLMRQLGGQDLSGIGFGLGVDRTLLALH
AEGKTVGETTRCDVFGVSLGEAAKLKVAMLAGQLRAAGVRVDLIYGDRGIRGAMRAAGRSGARIALIVDDCAIKADGVGV
RDLATGEQISVAVDSVVAEVISRIAPS
>Mature_426_residues
TESCTVFSFSGPKGIPDYFPPDSAQFVAVRDGLLTAARRAGYGDIELPVFEDTALFARGVGESTDVVAKEMYTFADRGDR
SVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRSL
GLDGFQLEITSLGDGTCRPQYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQAMTAAAPVLLDHLSDGAKQHFD
TVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLGAQSGIGGGGRYDGLMRQLGGQDLSGIGFGLGVDRTLLALHA
EGKTVGETTRCDVFGVSLGEAAKLKVAMLAGQLRAAGVRVDLIYGDRGIRGAMRAAGRSGARIALIVDDCAIKADGVGVR
DLATGEQISVAVDSVVAEVISRIAPS

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=471, Percent_Identity=22.7176220806794, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1788861, Length=412, Percent_Identity=41.747572815534, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=22.008547008547, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=22.008547008547, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=450, Percent_Identity=22, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_MYCLE (P46696)

Other databases:

- EMBL:   U00011
- EMBL:   AL023591
- EMBL:   AL583918
- PIR:   F86970
- PIR:   S72739
- RefSeq:   NP_301433.1
- ProteinModelPortal:   P46696
- SMR:   P46696
- EnsemblBacteria:   EBMYCT00000029485
- GeneID:   909246
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0494
- NMPDR:   fig|272631.1.peg.305
- Leproma:   ML0494
- GeneTree:   EBGT00050000017632
- HOGENOM:   HBG616575
- OMA:   WIRFASE
- ProtClustDB:   PRK00037
- BioCyc:   MLEP272631:ML0494-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 45695; Mature: 45563

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESCTVFSFSGPKGIPDYFPPDSAQFVAVRDGLLTAARRAGYGDIELPVFEDTALFARG
CCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEECEEECCHHHHHHC
VGESTDVVAKEMYTFADRGDRSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRY
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
ERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRSLGLDGFQLEITSLGDGTCRP
CCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEEECCCCCCCH
QYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQAMTAAAPVLLDHLSDGAKQHF
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLGAQSGIGGGGRYDGLMRQLGG
HHHHHHHHHHCCCEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
QDLSGIGFGLGVDRTLLALHAEGKTVGETTRCDVFGVSLGEAAKLKVAMLAGQLRAAGVR
CCCCCCEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHEEHHHHHHHHHHCCEE
VDLIYGDRGIRGAMRAAGRSGARIALIVDDCAIKADGVGVRDLATGEQISVAVDSVVAEV
EEEEECCCCCHHHHHHCCCCCCEEEEEEECCEEEECCCCCHHCCCCCHHHHHHHHHHHHH
ISRIAPS
HHHHCCC
>Mature Secondary Structure 
TESCTVFSFSGPKGIPDYFPPDSAQFVAVRDGLLTAARRAGYGDIELPVFEDTALFARG
CCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEECEEECCHHHHHHC
VGESTDVVAKEMYTFADRGDRSVTLRPEGTAGVVRAVIEHGLDRGALPVKLCYAGPFFRY
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
ERPQAGRCRQLQQVGVEAIGVDDPALDAEVITIADAGFRSLGLDGFQLEITSLGDGTCRP
CCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCHHHCCCCCEEEEEEECCCCCCCH
QYRKLLQEFLLQLDLDEDTRRRAELNPLRVLDDKRPQVQAMTAAAPVLLDHLSDGAKQHF
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DTVLAHLDALRVPYVINPRMVRGLDYYTKTTFEFVHPGLGAQSGIGGGGRYDGLMRQLGG
HHHHHHHHHHCCCEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
QDLSGIGFGLGVDRTLLALHAEGKTVGETTRCDVFGVSLGEAAKLKVAMLAGQLRAAGVR
CCCCCCEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCHHEEHHHHHHHHHHCCEE
VDLIYGDRGIRGAMRAAGRSGARIALIVDDCAIKADGVGVRDLATGEQISVAVDSVVAEV
EEEEECCCCCHHHHHHCCCCCCEEEEEEECCEEEECCCCCHHCCCCCHHHHHHHHHHHHH
ISRIAPS
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002