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The map label for this gene is ruvB

Identifier: 221229638

GI number: 221229638

Start: 582404

End: 583453

Strand: Direct

Name: ruvB

Synonym: MLBr_00483

Alternate gene names: 221229638

Gene position: 582404-583453 (Clockwise)

Preceding gene: 221229637

Following gene: 221229641

Centisome position: 17.82

GC content: 64.1

Gene sequence:

>1050_bases
ATGAGCGAGGACTACTTGGATCGGGATGTTTCCCCGGCGCTGACCGTCGGCGAAGCCGACATCGATGTCAGCCTGCGGCC
CCGCTCATTGCGGGAGTTCATCGGTCAGCCGCGGGTGCGTGAACAGCTCCAGCTGGTCATTGAAGGGGCCAAGAATCGTG
GCGCCACACCGGATCACATTCTGCTGTCCGGTCCTCCGGGGTTAGGTAAGACGTCGCTGGCAATGATCATCGCTGCTGAA
CTCGGTTCCTCGTTGCGGATGACGTCCGGTCCTGCGCTGGAACGCGCAGGTGACCTGGCAGTGATGCTATCCAACCTGGT
CGAGCACGACGTGTTGTTCATCGACGAAATCCACCGTATTGCCCGGCCTGCGGAGGAGATGCTGTACCTAGCGATGGAAG
ACTTTCGGGTCGACGTGATCGTCGGAAAAGGGCCTGGAGCGACATCGATTCCCTTAGAGGTCGCACCCTTTACTCTGGTC
GGCGCTACCACACGGTCCGGCGCGCTGACCGGACCGTTACGTGACCGGTTCGGGTTCACCGCACACATGGATTTCTACGA
GCCTACTGAGTTGGAGGGGGTGTTGGCTCGCGCGGCTGGGATTCTGGGCATCGAGTTGGGCGTCGAGGCAGGCGCGGAGA
TCGCTCGACGCTCGCGTGGGACGCCGCGGATCGCTAACCGGTTGCTGCGCCGGGTCCGTGACTTCGCCGAGGTCCGTGCT
GATGGCGTCATCACCCGCGATGTCGCTAAGGCGGCTCTGGCGGTCTACGACGTCGACGAGCTGGGCCTGGACCGACTGGA
TCGGGCGGTATTGTCGGCGCTGACTCGCAGCTTTGGCGGCGGTCCGGTCGGGGTTTCGACGTTAGCGGTGGCGGTCGGGG
AGGAGGCGACCACCGTAGAAGAGGTGTGTGAGCCGTTTCTGGTGCGTGCCGGCATGGTGGCACGGACCCCGCGCGGCCGG
GTGGCTACTGCGCAGGCCTGGACGTACCTGTGCATGACACCGCCGGTCGGGGTCACCGGGTTGAGTCAGCCGGGGTTATT
CGAGTCGTGA

Upstream 100 bases:

>100_bases
AAGCGACCGACCAGGTGCTCGACGGTGAACTTGGAAAAGATGGGGCGGTGGCAACGTCCAGCGCCTTGCGGGCTGCTCTG
TCGTTGCTGGGCAAGACCCG

Downstream 100 bases:

>100_bases
GGAGCGGGATGATGATTGCCGCCGGTTTGATCTGCGTCGCCCTCGCCGGCGGTACTGCACGTCTACGTCTTCACCATGGA
ATCGTTGACGTGGACTTCTG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSEDYLDRDVSPALTVGEADIDVSLRPRSLREFIGQPRVREQLQLVIEGAKNRGATPDHILLSGPPGLGKTSLAMIIAAE
LGSSLRMTSGPALERAGDLAVMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVIVGKGPGATSIPLEVAPFTLV
GATTRSGALTGPLRDRFGFTAHMDFYEPTELEGVLARAAGILGIELGVEAGAEIARRSRGTPRIANRLLRRVRDFAEVRA
DGVITRDVAKAALAVYDVDELGLDRLDRAVLSALTRSFGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMVARTPRGR
VATAQAWTYLCMTPPVGVTGLSQPGLFES

Sequences:

>Translated_349_residues
MSEDYLDRDVSPALTVGEADIDVSLRPRSLREFIGQPRVREQLQLVIEGAKNRGATPDHILLSGPPGLGKTSLAMIIAAE
LGSSLRMTSGPALERAGDLAVMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVIVGKGPGATSIPLEVAPFTLV
GATTRSGALTGPLRDRFGFTAHMDFYEPTELEGVLARAAGILGIELGVEAGAEIARRSRGTPRIANRLLRRVRDFAEVRA
DGVITRDVAKAALAVYDVDELGLDRLDRAVLSALTRSFGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMVARTPRGR
VATAQAWTYLCMTPPVGVTGLSQPGLFES
>Mature_348_residues
SEDYLDRDVSPALTVGEADIDVSLRPRSLREFIGQPRVREQLQLVIEGAKNRGATPDHILLSGPPGLGKTSLAMIIAAEL
GSSLRMTSGPALERAGDLAVMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVIVGKGPGATSIPLEVAPFTLVG
ATTRSGALTGPLRDRFGFTAHMDFYEPTELEGVLARAAGILGIELGVEAGAEIARRSRGTPRIANRLLRRVRDFAEVRAD
GVITRDVAKAALAVYDVDELGLDRLDRAVLSALTRSFGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMVARTPRGRV
ATAQAWTYLCMTPPVGVTGLSQPGLFES

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=329, Percent_Identity=54.1033434650456, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_MYCLB (B8ZUI2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503054.1
- ProteinModelPortal:   B8ZUI2
- SMR:   B8ZUI2
- EnsemblBacteria:   EBMYCT00000085776
- GeneID:   7325962
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015332
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF00004 AAA; PF05491 RuvB_C

EC number: =3.6.4.12

Molecular weight: Translated: 37266; Mature: 37135

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDYLDRDVSPALTVGEADIDVSLRPRSLREFIGQPRVREQLQLVIEGAKNRGATPDHI
CCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LLSGPPGLGKTSLAMIIAAELGSSLRMTSGPALERAGDLAVMLSNLVEHDVLFIDEIHRI
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ARPAEEMLYLAMEDFRVDVIVGKGPGATSIPLEVAPFTLVGATTRSGALTGPLRDRFGFT
HCCHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCEEEEECCCCCCCCCCCHHHHCCCE
AHMDFYEPTELEGVLARAAGILGIELGVEAGAEIARRSRGTPRIANRLLRRVRDFAEVRA
EEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DGVITRDVAKAALAVYDVDELGLDRLDRAVLSALTRSFGGGPVGVSTLAVAVGEEATTVE
CCCHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
EVCEPFLVRAGMVARTPRGRVATAQAWTYLCMTPPVGVTGLSQPGLFES
HHHHHHHHHCCHHEECCCCCEEEHHHEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEDYLDRDVSPALTVGEADIDVSLRPRSLREFIGQPRVREQLQLVIEGAKNRGATPDHI
CCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LLSGPPGLGKTSLAMIIAAELGSSLRMTSGPALERAGDLAVMLSNLVEHDVLFIDEIHRI
EEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ARPAEEMLYLAMEDFRVDVIVGKGPGATSIPLEVAPFTLVGATTRSGALTGPLRDRFGFT
HCCHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCEEEEECCCCCCCCCCCHHHHCCCE
AHMDFYEPTELEGVLARAAGILGIELGVEAGAEIARRSRGTPRIANRLLRRVRDFAEVRA
EEECCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DGVITRDVAKAALAVYDVDELGLDRLDRAVLSALTRSFGGGPVGVSTLAVAVGEEATTVE
CCCHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHH
EVCEPFLVRAGMVARTPRGRVATAQAWTYLCMTPPVGVTGLSQPGLFES
HHHHHHHHHCCHHEECCCCCEEEHHHEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA