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The map label for this gene is ispD

Identifier: 221229563

GI number: 221229563

Start: 410879

End: 411604

Strand: Direct

Name: ispD

Synonym: MLBr_00321

Alternate gene names: 221229563

Gene position: 410879-411604 (Clockwise)

Preceding gene: 221229562

Following gene: 221229564

Centisome position: 12.57

GC content: 62.26

Gene sequence:

>726_bases
ATGGCTGTGGCAACGGGCACTGTAGTTGCAGTGGTCCCGGCCGCCGGGGCGGGGAAGCGGCTAGCGGCCGGCATTCCGAA
GGCATTCTGTGAACTTGACGGGCGTACGCTTGTCGAACGTGCTGTCGTTGGCTTGCTGGAATCTGGCGTTGTTGACCATG
TTGTGGTGGCGGTGCCTGCTGATCGCATTGCCCAGACCCAGTGGGTGCTATCCCAACGGCTAGCCAATTCGGCGGGTCAA
CACGCCACAGTTGTGGCCGGCGGGGCCGACCGCACCAAATCGGTGTGTCAGGCTCTTGCGACCCTTCCCGCCCCGTCAAG
GGTGGGCGCACCCGAGTTTATATTGGTGCATGATGCCGCGCGGGCGCTGACACCAGCACGGTTAATCGTGCGGGTGGTTG
ATGCTTTGCGTGCTGGTCATACCGCGGTCGTTCCAGCGCTGCCACTGTCTGACACCATTAAAGCCGTGGACGCCAACGGA
ATGGTTCTTGGCACTCCGGCGCGGGTCGGCTTGCGGGCGGTGCAGACCCCGCAGGGGTTTGCTACCGAGCTGCTATGGTG
CGCTTATCAGCGTGGCCCCCATCTTGATGCCGTCGATTTCACCGACGATGCTTCCCTTGTCGAACATCTCGGTGGCCAGG
TTCAGGTGGTCGCCGGCGATCCACTGGCGTTCAAGATCACCACTCAGTTGGATCTGCTGCTGGCGAAGAAGATTCTGCGC
CGGTGA

Upstream 100 bases:

>100_bases
CGGCGAGTTGGCGTTGGCGGAGAGCACTGACGACGCCAAGGCGGAAACCATCCTCGACGAGGTTTTGGCCGCCGCTTCCT
GAATACCCTGAGGCTTGAGG

Downstream 100 bases:

>100_bases
GTGAACTGCCCCGGGTCGGTTTGGGTTTCGATGTCCACGTGTTTGAACCGGGGCGGCCGTGTTGGCTGGTGGGGCTGCTG
TTCCCGGACAACGACGGCTG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPADRIAQTQWVLSQRLANSAGQ
HATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAARALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANG
MVLGTPARVGLRAVQTPQGFATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILR
R

Sequences:

>Translated_241_residues
MAVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPADRIAQTQWVLSQRLANSAGQ
HATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAARALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANG
MVLGTPARVGLRAVQTPQGFATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILR
R
>Mature_240_residues
AVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPADRIAQTQWVLSQRLANSAGQH
ATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAARALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANGM
VLGTPARVGLRAVQTPQGFATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILRR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=238, Percent_Identity=27.3109243697479, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1789104, Length=244, Percent_Identity=35.655737704918, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_MYCLB (B8ZUA7)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502979.1
- ProteinModelPortal:   B8ZUA7
- SMR:   B8ZUA7
- EnsemblBacteria:   EBMYCT00000086177
- GeneID:   7326377
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016717
- HOGENOM:   HBG672839
- OMA:   SKVIVVC
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24980; Mature: 24849

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPA
CCEECCEEEEEEECCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHEEEEECH
DRIAQTQWVLSQRLANSAGQHATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECHH
RALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANGMVLGTPARVGLRAVQTPQGF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEECCCCEEEECCHHHCCEEECCCCHH
ATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILR
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHHHHHC
R
C
>Mature Secondary Structure 
AVATGTVVAVVPAAGAGKRLAAGIPKAFCELDGRTLVERAVVGLLESGVVDHVVVAVPA
CEECCEEEEEEECCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHEEEEECH
DRIAQTQWVLSQRLANSAGQHATVVAGGADRTKSVCQALATLPAPSRVGAPEFILVHDAA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECHH
RALTPARLIVRVVDALRAGHTAVVPALPLSDTIKAVDANGMVLGTPARVGLRAVQTPQGF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHEECCCCEEEECCHHHCCEEECCCCHH
ATELLWCAYQRGPHLDAVDFTDDASLVEHLGGQVQVVAGDPLAFKITTQLDLLLAKKILR
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA