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The map label for this gene is glnH [H]

Identifier: 221229552

GI number: 221229552

Start: 385666

End: 386646

Strand: Direct

Name: glnH [H]

Synonym: MLBr_00303

Alternate gene names: 221229552

Gene position: 385666-386646 (Clockwise)

Preceding gene: 221229551

Following gene: 221229553

Centisome position: 11.8

GC content: 59.33

Gene sequence:

>981_bases
GTGATGACGCGCCAGTTCAAGATCCAACGCGCCTGTAGTGTGCTCGCCACGGCGATGATGCTGGCCGCTTGCGGCCATGC
GGAACTCCTGACGGTGGCCAGCATGCCAACGCTGCCGCCACCCACACCGGTCGGCATGGAGCAAGTGCCGCCGGAACTCT
TACTGCCACAAGACAACACTGAGGAGGACTGCGACCCGACCGCCAGCCTGCGCCCCTTTCCCACAAAAGCCGAAGCAGAC
GCTGCGGTGGCCGAGATCCGCGCTCGTGGCCGGCTGATCGTCGGGCTCGAAATCGGCAGCAACTTGTTCAGCTTTCGCGA
CCCGATCACCGGCGAAATCACCGGGTTCGATATTGACATCGCCGGCGAGGTGGCGCGCGATATCTTCGGCACCCCATCGC
ATATGGAATACCGCATTCTGTCAACCGCAGCACGCATCACCGCGCTGCAGAAATCGGAGGTTGACATCATCGTTAAGACG
ATGACGATCACCTGTAAGCGACGCAAGCTGGTGAACTTCTCCACCGTCTACCTTGATGCCAAACAGCGTATCCTCGCTCC
GCGTGACTCACCAATCACGAAGCCATCTGACCTATCCAACAAACGAGTCTGCATGACACGTGGTACCACCTCGCTACATC
AAGTTAGCGAGGTGGTACCACCGCCGATCATTGTGTCAGCGGAGAACTGGGCTGACTGCCTAGTGGCAATGCAGCAGCGG
GAGATCGATGCCATCAGCACCGACGACGCTATCCTAGCCGGGCTAGTCGAGGAAGACCCCTACTTGCACATCGTCGGGCC
GAATATGGCCACCCAGCCCTATGGCATAGGGATTAACTTGAACAACACCACGTTGGTTCGGTTCGTCAACGGCACACTAG
AGCGCATCCGTAGGGACGGCACCTGGATCACGTTGTACCGAAAGTGGTTAACGGTCCTTGGGCCGGCGCCAGCTCCGCCC
ACACCGAGGTATGTGGATTGA

Upstream 100 bases:

>100_bases
GCAGCGGGTTATCCGGTGCCCAAATCGGGGGCATGGTACTCAGCCTGGGCGTCGCCATTGCGGTAGCCCTCGGCCTGTGG
CCGCGGTTGAAGGAATATCG

Downstream 100 bases:

>100_bases
TGGCCGAGTCCATGAAGAGAGAACACATGGACCACGACACCGAAGACGTGGGCCAGGCCGCCCAGCGGGCAGACCCGCCG
TCCGGCACAACGGAAGGGCG

Product: putative glutamine-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MMTRQFKIQRACSVLATAMMLAACGHAELLTVASMPTLPPPTPVGMEQVPPELLLPQDNTEEDCDPTASLRPFPTKAEAD
AAVAEIRARGRLIVGLEIGSNLFSFRDPITGEITGFDIDIAGEVARDIFGTPSHMEYRILSTAARITALQKSEVDIIVKT
MTITCKRRKLVNFSTVYLDAKQRILAPRDSPITKPSDLSNKRVCMTRGTTSLHQVSEVVPPPIIVSAENWADCLVAMQQR
EIDAISTDDAILAGLVEEDPYLHIVGPNMATQPYGIGINLNNTTLVRFVNGTLERIRRDGTWITLYRKWLTVLGPAPAPP
TPRYVD

Sequences:

>Translated_326_residues
MMTRQFKIQRACSVLATAMMLAACGHAELLTVASMPTLPPPTPVGMEQVPPELLLPQDNTEEDCDPTASLRPFPTKAEAD
AAVAEIRARGRLIVGLEIGSNLFSFRDPITGEITGFDIDIAGEVARDIFGTPSHMEYRILSTAARITALQKSEVDIIVKT
MTITCKRRKLVNFSTVYLDAKQRILAPRDSPITKPSDLSNKRVCMTRGTTSLHQVSEVVPPPIIVSAENWADCLVAMQQR
EIDAISTDDAILAGLVEEDPYLHIVGPNMATQPYGIGINLNNTTLVRFVNGTLERIRRDGTWITLYRKWLTVLGPAPAPP
TPRYVD
>Mature_326_residues
MMTRQFKIQRACSVLATAMMLAACGHAELLTVASMPTLPPPTPVGMEQVPPELLLPQDNTEEDCDPTASLRPFPTKAEAD
AAVAEIRARGRLIVGLEIGSNLFSFRDPITGEITGFDIDIAGEVARDIFGTPSHMEYRILSTAARITALQKSEVDIIVKT
MTITCKRRKLVNFSTVYLDAKQRILAPRDSPITKPSDLSNKRVCMTRGTTSLHQVSEVVPPPIIVSAENWADCLVAMQQR
EIDAISTDDAILAGLVEEDPYLHIVGPNMATQPYGIGINLNNTTLVRFVNGTLERIRRDGTWITLYRKWLTVLGPAPAPP
TPRYVD

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=204, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787085, Length=204, Percent_Identity=25.9803921568627, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787031, Length=234, Percent_Identity=23.5042735042735, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=24.8908296943231, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 35806; Mature: 35806

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTRQFKIQRACSVLATAMMLAACGHAELLTVASMPTLPPPTPVGMEQVPPELLLPQDNT
CCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCC
EEDCDPTASLRPFPTKAEADAAVAEIRARGRLIVGLEIGSNLFSFRDPITGEITGFDIDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCCEEEEEEEEE
AGEVARDIFGTPSHMEYRILSTAARITALQKSEVDIIVKTMTITCKRRKLVNFSTVYLDA
HHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCEEEEEEEEEEEC
KQRILAPRDSPITKPSDLSNKRVCMTRGTTSLHQVSEVVPPPIIVSAENWADCLVAMQQR
HHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
EIDAISTDDAILAGLVEEDPYLHIVGPNMATQPYGIGINLNNTTLVRFVNGTLERIRRDG
CCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHCCC
TWITLYRKWLTVLGPAPAPPTPRYVD
CEEEEEHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MMTRQFKIQRACSVLATAMMLAACGHAELLTVASMPTLPPPTPVGMEQVPPELLLPQDNT
CCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCC
EEDCDPTASLRPFPTKAEADAAVAEIRARGRLIVGLEIGSNLFSFRDPITGEITGFDIDI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCCEEEEEEEEE
AGEVARDIFGTPSHMEYRILSTAARITALQKSEVDIIVKTMTITCKRRKLVNFSTVYLDA
HHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCEEEEEEEEEEEC
KQRILAPRDSPITKPSDLSNKRVCMTRGTTSLHQVSEVVPPPIIVSAENWADCLVAMQQR
HHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
EIDAISTDDAILAGLVEEDPYLHIVGPNMATQPYGIGINLNNTTLVRFVNGTLERIRRDG
CCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHCCC
TWITLYRKWLTVLGPAPAPPTPRYVD
CEEEEEHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]