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The map label for this gene is fadD [C]

Identifier: 221229517

GI number: 221229517

Start: 318236

End: 319870

Strand: Direct

Name: fadD [C]

Synonym: MLBr_00243

Alternate gene names: 221229517

Gene position: 318236-319870 (Clockwise)

Preceding gene: 221229516

Following gene: 221229524

Centisome position: 9.74

GC content: 60.73

Gene sequence:

>1635_bases
GTGAGCAAGTTTACCGAGAAGATGTTCCGCAACGCCCGTGAGTCCTCAAAGGGCATAGTGACAGGTGAGCCACACAATCC
GGTTCGTCACACCTGGGGTCAGGTGCATGAGCGCGCTAGCCGAATAGCCGGTGGGTTGGCAGGTGTCGGTGTTGGTCACG
GCGACGCGGTCGGTGTGCTCGTTGGCGTGCCGGTGGAGATTGCTCCGACCGCACAAGGGCTGTGGATGCGCGGCGCTAGC
CTAACCATGCTGCATCAGCCCACCCCGCGGACCGACCTGGCGGTATGGGAAGAAGACACCACCACCGTCATCGACATGAT
CGAAGCCAAAGCGGTCATCATCTCCGACCCGTTTATGGCGGTAGCGCCAATTCTGGAGGCTCGCGGTGTTAAGGTGGTGA
CCGTCGAGCAGTTGTTGGCAGCCGATCCGGTCGACCCGATCGAAACTGACGAGGACGATCTGGCGCTGATGCAATTGACA
TCCGGCTCCACGGGTTCGCCCAAAGCTGTCCAGATTACTCACCGTAACGTGTATTCCAATGTCGAAGCTATGTTTATCGG
CGTCAGGTACAACGTCGAGAAAGACGTGATGATCAGCTGGCTGCCGTGCTTTCATGACATGGGGATGATCGGGTTTTTGA
CCGTGCCGATGTACTTCGGCGCCGAGCTGGTCAAAGTCACCCCGATAGACTTCTTGCGCGACACTTTGCTGTGGGCCAAG
CTGATCGACAAGTACAAGGGCACCGTCATCTGCGGCCCGAACTTTGCCTACTCGCTGTTTACCAAGCGGCTGCGCAAGCA
AGCCAAACCTGGCCAGTTCGACTTGTCGACTCTGCGGATTGCGATGTCAGGTGCTGAACCGGTGGACCCGGCGGACGTCG
AGGACTTGATCGACGCGGGCAGGCCGTTCGGCTTCAGGCCGGAGGCCATCCTGCCCGCATACGGAATGGCCGAGACCACA
CTGGCGGTCTCGTTCTCACCGGTTGGAGAGGGGTTGCTTGTTGACGAGGTGGATGCCGACCTGTTGGCCGCGCTGCGCCG
TGCGGTGCCAGCCACCAAGGGCAACATCCGCCGGCTGGTTTCACTCGGTCCGCTGCTGGACGGCCTGGAGGTCCGTATCG
TTGATGAGGATGGCAATGTGCTTCCGTCCCGAGGTGTCGGCGTGATCGAGTTGCGTGGGGAGTCGCTGACCCCGGGCTAT
ATCACGATGGGCGGCTTCATCTCGGCCCAAGATGAGCACGGTTGGTATGACACTGGGGATCTCGGCTATCAGATGGAAAA
CGGTCACATTGTAGTGTGTGGTCGGGTCAAGGACGTCATCATCATGGCTGGCCGCAATGTATACCCGACCGACATCGAAC
GAGCGGCGGGTCGGGTTGATGGTGTCCGCCCGGGTTGTGCAGTGGCCGTACGACTCGACGCCGGGCATTCGCGTGAGACC
TTCGCTGTCGCTGTCGAGTCGAACGCTTTCGAGGACACCGCCGAAGTGCGCCGCATTGAGCATCAGGTGGCGCACGAGGT
GCTCAAAGAGGTGGATGTGCGGCCACGCAACGTGGTGGTTCTCGGCCCGGGAACCATTCCGAAGACGCCGTCGGGCAAAC
TGCGGCGGGCCAGTTCCGTTACCCTCGTCACCTAG

Upstream 100 bases:

>100_bases
CCCCAACTAGTGCACGTGTGCTTGCTACACAGCGACGTCCAGCAGGCTGAACATGATATCCCAAGGCCCTTGGGGAAAAT
ATCGGAACCGAGGAGGGGTT

Downstream 100 bases:

>100_bases
TGTTCGACCGGCAGCCGAGCTGATGAAAACTGCCATGGTTTCCGGCGTGTCGTTTTACGGAAATGCGGAAATGCGCCCTC
GTGGGGTGCAGCTGAGGACG

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSKFTEKMFRNARESSKGIVTGEPHNPVRHTWGQVHERASRIAGGLAGVGVGHGDAVGVLVGVPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAVWEEDTTTVIDMIEAKAVIISDPFMAVAPILEARGVKVVTVEQLLAADPVDPIETDEDDLALMQLT
SGSTGSPKAVQITHRNVYSNVEAMFIGVRYNVEKDVMISWLPCFHDMGMIGFLTVPMYFGAELVKVTPIDFLRDTLLWAK
LIDKYKGTVICGPNFAYSLFTKRLRKQAKPGQFDLSTLRIAMSGAEPVDPADVEDLIDAGRPFGFRPEAILPAYGMAETT
LAVSFSPVGEGLLVDEVDADLLAALRRAVPATKGNIRRLVSLGPLLDGLEVRIVDEDGNVLPSRGVGVIELRGESLTPGY
ITMGGFISAQDEHGWYDTGDLGYQMENGHIVVCGRVKDVIIMAGRNVYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRET
FAVAVESNAFEDTAEVRRIEHQVAHEVLKEVDVRPRNVVVLGPGTIPKTPSGKLRRASSVTLVT

Sequences:

>Translated_544_residues
MSKFTEKMFRNARESSKGIVTGEPHNPVRHTWGQVHERASRIAGGLAGVGVGHGDAVGVLVGVPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAVWEEDTTTVIDMIEAKAVIISDPFMAVAPILEARGVKVVTVEQLLAADPVDPIETDEDDLALMQLT
SGSTGSPKAVQITHRNVYSNVEAMFIGVRYNVEKDVMISWLPCFHDMGMIGFLTVPMYFGAELVKVTPIDFLRDTLLWAK
LIDKYKGTVICGPNFAYSLFTKRLRKQAKPGQFDLSTLRIAMSGAEPVDPADVEDLIDAGRPFGFRPEAILPAYGMAETT
LAVSFSPVGEGLLVDEVDADLLAALRRAVPATKGNIRRLVSLGPLLDGLEVRIVDEDGNVLPSRGVGVIELRGESLTPGY
ITMGGFISAQDEHGWYDTGDLGYQMENGHIVVCGRVKDVIIMAGRNVYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRET
FAVAVESNAFEDTAEVRRIEHQVAHEVLKEVDVRPRNVVVLGPGTIPKTPSGKLRRASSVTLVT
>Mature_543_residues
SKFTEKMFRNARESSKGIVTGEPHNPVRHTWGQVHERASRIAGGLAGVGVGHGDAVGVLVGVPVEIAPTAQGLWMRGASL
TMLHQPTPRTDLAVWEEDTTTVIDMIEAKAVIISDPFMAVAPILEARGVKVVTVEQLLAADPVDPIETDEDDLALMQLTS
GSTGSPKAVQITHRNVYSNVEAMFIGVRYNVEKDVMISWLPCFHDMGMIGFLTVPMYFGAELVKVTPIDFLRDTLLWAKL
IDKYKGTVICGPNFAYSLFTKRLRKQAKPGQFDLSTLRIAMSGAEPVDPADVEDLIDAGRPFGFRPEAILPAYGMAETTL
AVSFSPVGEGLLVDEVDADLLAALRRAVPATKGNIRRLVSLGPLLDGLEVRIVDEDGNVLPSRGVGVIELRGESLTPGYI
TMGGFISAQDEHGWYDTGDLGYQMENGHIVVCGRVKDVIIMAGRNVYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRETF
AVAVESNAFEDTAEVRRIEHQVAHEVLKEVDVRPRNVVVLGPGTIPKTPSGKLRRASSVTLVT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=474, Percent_Identity=22.7848101265823, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788107, Length=389, Percent_Identity=26.4781491002571, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693145, Length=395, Percent_Identity=27.5949367088608, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=26.9021739130435, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=474, Percent_Identity=23.4177215189873, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=474, Percent_Identity=23.4177215189873, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=474, Percent_Identity=23.4177215189873, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=329, Percent_Identity=28.2674772036474, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=395, Percent_Identity=25.0632911392405, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=404, Percent_Identity=24.5049504950495, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=315, Percent_Identity=24.7619047619048, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=342, Percent_Identity=25.1461988304094, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=322, Percent_Identity=24.8447204968944, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581924, Length=304, Percent_Identity=26.6447368421053, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130357, Length=519, Percent_Identity=23.5067437379576, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18859661, Length=322, Percent_Identity=24.5341614906832, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=550, Percent_Identity=24.3636363636364, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58846; Mature: 58715

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFTEKMFRNARESSKGIVTGEPHNPVRHTWGQVHERASRIAGGLAGVGVGHGDAVGVL
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEE
VGVPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWEEDTTTVIDMIEAKAVIISDPFMA
EECCEEECCCCCCEEECCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCEEEEECCHHH
VAPILEARGVKVVTVEQLLAADPVDPIETDEDDLALMQLTSGSTGSPKAVQITHRNVYSN
HHHHHHHCCEEEEEHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCHHHCC
VEAMFIGVRYNVEKDVMISWLPCFHDMGMIGFLTVPMYFGAELVKVTPIDFLRDTLLWAK
CEEEEEEEEECCCCCCEEEEHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTVICGPNFAYSLFTKRLRKQAKPGQFDLSTLRIAMSGAEPVDPADVEDLIDAG
HHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHCC
RPFGFRPEAILPAYGMAETTLAVSFSPVGEGLLVDEVDADLLAALRRAVPATKGNIRRLV
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
SLGPLLDGLEVRIVDEDGNVLPSRGVGVIELRGESLTPGYITMGGFISAQDEHGWYDTGD
HHCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCEEEECCCCCCCCCCC
LGYQMENGHIVVCGRVKDVIIMAGRNVYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRET
CCEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCE
FAVAVESNAFEDTAEVRRIEHQVAHEVLKEVDVRPRNVVVLGPGTIPKTPSGKLRRASSV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
TLVT
EEEC
>Mature Secondary Structure 
SKFTEKMFRNARESSKGIVTGEPHNPVRHTWGQVHERASRIAGGLAGVGVGHGDAVGVL
CHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEE
VGVPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAVWEEDTTTVIDMIEAKAVIISDPFMA
EECCEEECCCCCCEEECCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCEEEEECCHHH
VAPILEARGVKVVTVEQLLAADPVDPIETDEDDLALMQLTSGSTGSPKAVQITHRNVYSN
HHHHHHHCCEEEEEHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCHHHCC
VEAMFIGVRYNVEKDVMISWLPCFHDMGMIGFLTVPMYFGAELVKVTPIDFLRDTLLWAK
CEEEEEEEEECCCCCCEEEEHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTVICGPNFAYSLFTKRLRKQAKPGQFDLSTLRIAMSGAEPVDPADVEDLIDAG
HHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHCC
RPFGFRPEAILPAYGMAETTLAVSFSPVGEGLLVDEVDADLLAALRRAVPATKGNIRRLV
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
SLGPLLDGLEVRIVDEDGNVLPSRGVGVIELRGESLTPGYITMGGFISAQDEHGWYDTGD
HHCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEECCEEEECCCCCCCCCCC
LGYQMENGHIVVCGRVKDVIIMAGRNVYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRET
CCEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCE
FAVAVESNAFEDTAEVRRIEHQVAHEVLKEVDVRPRNVVVLGPGTIPKTPSGKLRRASSV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
TLVT
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA