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The map label for this gene is lysS

Identifier: 221229508

GI number: 221229508

Start: 303755

End: 305278

Strand: Direct

Name: lysS

Synonym: MLBr_00233

Alternate gene names: 221229508

Gene position: 303755-305278 (Clockwise)

Preceding gene: 221229507

Following gene: 221229509

Centisome position: 9.29

GC content: 54.92

Gene sequence:

>1524_bases
GTGAACGCTGACCCGTTGGAGACTGACGCTGCTCTTCCTGAGCAGTTTCGGATTCGCCGGGACAAGCGTGCTCGTTTGTT
GGCGGAGGGGCGTGACCCGTATCCGGTCGCCATTGAACGCACCCATACCCTAGCTGAGGTTCGCGCAGCCTATCCCGACC
TGGCGACCGACTCCGCCACCGATGACATCGTTGGTATCGCGGGTAGGGTAATATTCGCGCGTAATTCGGGGAAACTGTGC
TTTGCGACACTGCAGGACGGTGACGGCACCAACTTGCAGGTGATGATTAGTCTCAACAAGGTTGGTTCCGAAACGCTTGA
CGCGTGGAAAGTCGACGTCGATCTTGGCGACATTGTTTATGTACACGGGAACGTGATTAGCTCGCGTAGCGGCGAGTTAT
CAGTTCTTGCTGATTCCTGGCAGATGGTATCTAAGTCGTTGCGGCCACTGCCCGTCGCGCATAAAGAGATGAGTGAAGAG
TCGCGGGTGCGACAGCGTTATGTTGACCTTATTGTCTGTCCGCAAGTGCGAATAGTAGCTCATCAGAGAATTGCCGTTAT
CCGCGCCATACGGACTGCGTTAGAACGACGCGGTTTTCTCGAAGTTGAGACGCCTATGTTGCAGACTCTGGCCGGTGGCG
CTGCGGCCCGGCCATTCGTCACACATTCCAATGCCCTGGACATCGATCTGTACCTGCGTATCGCACCTGAACTGTTCCTC
AAGCGATGCATCGTCGGTGGTTTCGACAAGGTCTTCGAACTTAATCGAGTGTTTCGAAACGAAGGCGCCGATTCTACGCA
TTCCCCGGAATTTTCCATGCTAGAGACCTACCAGACCTACGGAACCTATGACGATTCGGCGGTCGTTACCCGAGAAATTA
TTCAAGAGGTGGCCGATGAGGCGATCGGGACCAGACAACTGCAGATGCCTGATGACAGCGTCTACGACATAGATGGAGAA
TGGGAGACGATACAAATGTATCCGTCTCTGTCGGCTGTGTTGGGTGAAGAAATCACGCCGCAGACGTCAGTCGATCGGCT
GCGAGCCATCGCGGATCGGCTTGGCCGTGGGATTGGTCCAGAGATTCTTGACAAGCCCAGTTACGGACATGGAAGACTCG
TCGAGCAACTGTGGGAGTACACAGTGGGCAACACCCTGAGCGCGCCCACATTTGTCAAAGATTTTCCGGTCGAAACAACT
CCTTTGACCCGTCAGCATCGAAGCATCCCCGGAGTGACCGAGAAGTGGGATCTCTACCTGCGTGGAGTTGAACTGGCCAC
GGGGTACTCCGAATTAAACGATCCGGTCGTGCAGCGGGAAAGATTTGGTCAACAAGTCCGTGCTGCAGCCGCCGAGGAAG
ATGAGGCGATGGCACTTGATGAGGAATTTCTGGCGGCACTGGAGTACGCGATGCCGCCATGCACTGGAACTGGAATGGGT
ATCGACCGGTTGTTGATGTCTTTGACTGGACTGTCAATTAGGGAGACTGTTTTGTTCCCGATTGTTCGGCCGCACTCCAA
CTGA

Upstream 100 bases:

>100_bases
GGTTGAAGACGGCGCGCTGACGACGTCTGGCAGTGCGCACGTGAAGTTGTTCGACGCCACTGAACAGTGCCCGGCGTGAG
GTCATTTAAGCTGGCACGTC

Downstream 100 bases:

>100_bases
ACTGTTCGTGTTGTGTCGAGATTGAGTATGCGGTGTTCTTATGGCAGATTAGTATCTGGGGAGGTTGATCCAAACTGCTC
AGGAAGAAACGTGAGGGTAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNADPLETDAALPEQFRIRRDKRARLLAEGRDPYPVAIERTHTLAEVRAAYPDLATDSATDDIVGIAGRVIFARNSGKLC
FATLQDGDGTNLQVMISLNKVGSETLDAWKVDVDLGDIVYVHGNVISSRSGELSVLADSWQMVSKSLRPLPVAHKEMSEE
SRVRQRYVDLIVCPQVRIVAHQRIAVIRAIRTALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFL
KRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTREIIQEVADEAIGTRQLQMPDDSVYDIDGE
WETIQMYPSLSAVLGEEITPQTSVDRLRAIADRLGRGIGPEILDKPSYGHGRLVEQLWEYTVGNTLSAPTFVKDFPVETT
PLTRQHRSIPGVTEKWDLYLRGVELATGYSELNDPVVQRERFGQQVRAAAAEEDEAMALDEEFLAALEYAMPPCTGTGMG
IDRLLMSLTGLSIRETVLFPIVRPHSN

Sequences:

>Translated_507_residues
MNADPLETDAALPEQFRIRRDKRARLLAEGRDPYPVAIERTHTLAEVRAAYPDLATDSATDDIVGIAGRVIFARNSGKLC
FATLQDGDGTNLQVMISLNKVGSETLDAWKVDVDLGDIVYVHGNVISSRSGELSVLADSWQMVSKSLRPLPVAHKEMSEE
SRVRQRYVDLIVCPQVRIVAHQRIAVIRAIRTALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFL
KRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTREIIQEVADEAIGTRQLQMPDDSVYDIDGE
WETIQMYPSLSAVLGEEITPQTSVDRLRAIADRLGRGIGPEILDKPSYGHGRLVEQLWEYTVGNTLSAPTFVKDFPVETT
PLTRQHRSIPGVTEKWDLYLRGVELATGYSELNDPVVQRERFGQQVRAAAAEEDEAMALDEEFLAALEYAMPPCTGTGMG
IDRLLMSLTGLSIRETVLFPIVRPHSN
>Mature_507_residues
MNADPLETDAALPEQFRIRRDKRARLLAEGRDPYPVAIERTHTLAEVRAAYPDLATDSATDDIVGIAGRVIFARNSGKLC
FATLQDGDGTNLQVMISLNKVGSETLDAWKVDVDLGDIVYVHGNVISSRSGELSVLADSWQMVSKSLRPLPVAHKEMSEE
SRVRQRYVDLIVCPQVRIVAHQRIAVIRAIRTALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFL
KRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTREIIQEVADEAIGTRQLQMPDDSVYDIDGE
WETIQMYPSLSAVLGEEITPQTSVDRLRAIADRLGRGIGPEILDKPSYGHGRLVEQLWEYTVGNTLSAPTFVKDFPVETT
PLTRQHRSIPGVTEKWDLYLRGVELATGYSELNDPVVQRERFGQQVRAAAAEEDEAMALDEEFLAALEYAMPPCTGTGMG
IDRLLMSLTGLSIRETVLFPIVRPHSN

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=38.0116959064327, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=38.0116959064327, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI45439306, Length=475, Percent_Identity=21.8947368421053, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1790571, Length=503, Percent_Identity=38.3697813121272, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=38.2470119521912, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI87082379, Length=337, Percent_Identity=31.1572700296736, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788173, Length=245, Percent_Identity=27.7551020408163, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=514, Percent_Identity=37.15953307393, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17535927, Length=514, Percent_Identity=37.15953307393, Blast_Score=321, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=490, Percent_Identity=37.7551020408163, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6320242, Length=515, Percent_Identity=36.8932038834951, Blast_Score=294, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6324256, Length=468, Percent_Identity=29.7008547008547, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24640849, Length=495, Percent_Identity=37.7777777777778, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24640851, Length=495, Percent_Identity=37.7777777777778, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24584738, Length=273, Percent_Identity=27.1062271062271, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_MYCLE (P46861)

Other databases:

- EMBL:   AL583917
- EMBL:   M67510
- PIR:   A43601
- PIR:   A86938
- RefSeq:   NP_301293.1
- ProteinModelPortal:   P46861
- SMR:   P46861
- EnsemblBacteria:   EBMYCT00000029041
- GeneID:   908650
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0233
- NMPDR:   fig|272631.1.peg.165
- Leproma:   ML0233
- GeneTree:   EBGT00050000017004
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   MLEP272631:ML0233-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56422; Mature: 56422

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADPLETDAALPEQFRIRRDKRARLLAEGRDPYPVAIERTHTLAEVRAAYPDLATDSAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCC
DDIVGIAGRVIFARNSGKLCFATLQDGDGTNLQVMISLNKVGSETLDAWKVDVDLGDIVY
CHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEE
VHGNVISSRSGELSVLADSWQMVSKSLRPLPVAHKEMSEESRVRQRYVDLIVCPQVRIVA
EECCEEECCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEECCCHHHHH
HQRIAVIRAIRTALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFL
HHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCEEEEEEEEHHHHHHH
KRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTREIIQEVADE
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIGTRQLQMPDDSVYDIDGEWETIQMYPSLSAVLGEEITPQTSVDRLRAIADRLGRGIGP
HHCCEEEECCCCCEEECCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
EILDKPSYGHGRLVEQLWEYTVGNTLSAPTFVKDFPVETTPLTRQHRSIPGVTEKWDLYL
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCEEEE
RGVELATGYSELNDPVVQRERFGQQVRAAAAEEDEAMALDEEFLAALEYAMPPCTGTGMG
EEEHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
IDRLLMSLTGLSIRETVLFPIVRPHSN
HHHHHHHHCCCCHHHHHEEEEECCCCC
>Mature Secondary Structure
MNADPLETDAALPEQFRIRRDKRARLLAEGRDPYPVAIERTHTLAEVRAAYPDLATDSAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCC
DDIVGIAGRVIFARNSGKLCFATLQDGDGTNLQVMISLNKVGSETLDAWKVDVDLGDIVY
CHHHHCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEE
VHGNVISSRSGELSVLADSWQMVSKSLRPLPVAHKEMSEESRVRQRYVDLIVCPQVRIVA
EECCEEECCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEECCCHHHHH
HQRIAVIRAIRTALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFL
HHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCEEEEEEEEHHHHHHH
KRCIVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQTYGTYDDSAVVTREIIQEVADE
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AIGTRQLQMPDDSVYDIDGEWETIQMYPSLSAVLGEEITPQTSVDRLRAIADRLGRGIGP
HHCCEEEECCCCCEEECCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCH
EILDKPSYGHGRLVEQLWEYTVGNTLSAPTFVKDFPVETTPLTRQHRSIPGVTEKWDLYL
HHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCEEEE
RGVELATGYSELNDPVVQRERFGQQVRAAAAEEDEAMALDEEFLAALEYAMPPCTGTGMG
EEEHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
IDRLLMSLTGLSIRETVLFPIVRPHSN
HHHHHHHHCCCCHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002; 1840579