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The map label for this gene is mesJ

Identifier: 221229493

GI number: 221229493

Start: 284595

End: 285566

Strand: Direct

Name: mesJ

Synonym: MLBr_00213

Alternate gene names: 221229493

Gene position: 284595-285566 (Clockwise)

Preceding gene: 221229492

Following gene: 221229494

Centisome position: 8.71

GC content: 63.89

Gene sequence:

>972_bases
ATGGATCGACAGGGTGCTGTAGGGCAGCTGCGCAGGACAGTCGAAGAGTTTGCTGGTACCTATTGTGATATCGACGATCA
ATGGTGCGTAGCGCTCTCCGGCGGCTCGGACTCGTTGGCGCTCACTGCGGTTGCGGCCCAACTACGCTCTACGACCGCAT
TTATCGTGGATCACGGTTTGCAGTCTGACTCGGCCGCTGTCGCAGAAACCGCTCGAAAGCAAGCTATCTCATTGGGATGT
GTTGACGCACAAGTGCTTTGTGTGCAGATAGATCCACCGTTTGGTCCCAAAGGCGGGCTCGAGGCAGCGGCCCGTGCTGC
CCGATACGCTGTGTTGGGCGCCAATCGTAATGGCCCGGTGTTGTTAGCCCACACTCTCGACGATCAAGCTGAGACGGTGC
TGCTTGGCTTGGGCCGCGGTTCTGGGGCGCGCTCAATCGCTGGAATGCGGCCGCACGATCCGCCGTGGTGCCGGCCGTTA
CTGGGTGTGCGGCGCAGCGTGCCGCGTGCCGCGTGCCACGAGCTGGGCCTAAACGCTTGGCAGGATCCGCACAACACAGA
CTGTCGTTACACCCGAACCCGGCTGCGCCTCGAGGTGCTGCCGCTATTGGAGGACGTGCTAGGTGGTGGGGTCGTCGAGG
CGTTGGCCCGCACTGCGACGGCGTTGCGCGAGGACACTGAACTGATCGACACGTTCGCCGCACAGGCGCTGCCCAACATC
TGTGCCGGGTCGGGGCTACATGCGCGGGCTCTGGCCGCACTTCCCGACGCGGTTCGACGCCGGGTTATCCGTGGCTGGCT
GTTGAATGGAGGCGCGACCGGGCTGACCGACAAGCAGATCCGTGGAGTGGACACACTGGTTACCGCGTGGCGCGGGCAAG
GCGGGGTGGCGGTGGGATCCACCTTGCGCGGACAGCGTTTGATCGCCAGCCGGTACGACGGCGTACTGACCTTGCACTGC
GCGCCCGTTTAG

Upstream 100 bases:

>100_bases
GTTTGTCGATCACGTGGTGGGTCGGAATGGTACGGTTCAACACGATTTGTTCAGGTCTCGAAACGCTGCTGTTGTCTGCC
GAAATCGAAGACCCGCAGAG

Downstream 100 bases:

>100_bases
CGGCCCTTAGCGGGTCCTGATGCCGGGTCGCCGTGGTTTTTCGCAGGGCAGCATGGCACGCTTTGCTCGTGGCCCAGAGC
TTCTCGGCGATTAACCCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDRQGAVGQLRRTVEEFAGTYCDIDDQWCVALSGGSDSLALTAVAAQLRSTTAFIVDHGLQSDSAAVAETARKQAISLGC
VDAQVLCVQIDPPFGPKGGLEAAARAARYAVLGANRNGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPHDPPWCRPL
LGVRRSVPRAACHELGLNAWQDPHNTDCRYTRTRLRLEVLPLLEDVLGGGVVEALARTATALREDTELIDTFAAQALPNI
CAGSGLHARALAALPDAVRRRVIRGWLLNGGATGLTDKQIRGVDTLVTAWRGQGGVAVGSTLRGQRLIASRYDGVLTLHC
APV

Sequences:

>Translated_323_residues
MDRQGAVGQLRRTVEEFAGTYCDIDDQWCVALSGGSDSLALTAVAAQLRSTTAFIVDHGLQSDSAAVAETARKQAISLGC
VDAQVLCVQIDPPFGPKGGLEAAARAARYAVLGANRNGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPHDPPWCRPL
LGVRRSVPRAACHELGLNAWQDPHNTDCRYTRTRLRLEVLPLLEDVLGGGVVEALARTATALREDTELIDTFAAQALPNI
CAGSGLHARALAALPDAVRRRVIRGWLLNGGATGLTDKQIRGVDTLVTAWRGQGGVAVGSTLRGQRLIASRYDGVLTLHC
APV
>Mature_323_residues
MDRQGAVGQLRRTVEEFAGTYCDIDDQWCVALSGGSDSLALTAVAAQLRSTTAFIVDHGLQSDSAAVAETARKQAISLGC
VDAQVLCVQIDPPFGPKGGLEAAARAARYAVLGANRNGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPHDPPWCRPL
LGVRRSVPRAACHELGLNAWQDPHNTDCRYTRTRLRLEVLPLLEDVLGGGVVEALARTATALREDTELIDTFAAQALPNI
CAGSGLHARALAALPDAVRRRVIRGWLLNGGATGLTDKQIRGVDTLVTAWRGQGGVAVGSTLRGQRLIASRYDGVLTLHC
APV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=252, Percent_Identity=33.7301587301587, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_MYCLE (O69538)

Other databases:

- EMBL:   AL023093
- EMBL:   AL583917
- PIR:   E86935
- RefSeq:   NP_301278.1
- ProteinModelPortal:   O69538
- SMR:   O69538
- EnsemblBacteria:   EBMYCT00000029307
- GeneID:   909579
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0213
- NMPDR:   fig|272631.1.peg.150
- Leproma:   ML0213
- GeneTree:   EBGT00050000016642
- HOGENOM:   HBG692699
- OMA:   QDPHNSD
- ProtClustDB:   CLSK792603
- BioCyc:   MLEP272631:ML0213-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946

EC number: NA

Molecular weight: Translated: 34113; Mature: 34113

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQGAVGQLRRTVEEFAGTYCDIDDQWCVALSGGSDSLALTAVAAQLRSTTAFIVDHGL
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCC
QSDSAAVAETARKQAISLGCVDAQVLCVQIDPPFGPKGGLEAAARAARYAVLGANRNGPV
CCCHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCE
LLAHTLDDQAETVLLGLGRGSGARSIAGMRPHDPPWCRPLLGVRRSVPRAACHELGLNAW
EEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCC
QDPHNTDCRYTRTRLRLEVLPLLEDVLGGGVVEALARTATALREDTELIDTFAAQALPNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
CAGSGLHARALAALPDAVRRRVIRGWLLNGGATGLTDKQIRGVDTLVTAWRGQGGVAVGS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCCCEEECC
TLRGQRLIASRYDGVLTLHCAPV
CCCCHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MDRQGAVGQLRRTVEEFAGTYCDIDDQWCVALSGGSDSLALTAVAAQLRSTTAFIVDHGL
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCC
QSDSAAVAETARKQAISLGCVDAQVLCVQIDPPFGPKGGLEAAARAARYAVLGANRNGPV
CCCHHHHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCHHHHHHHHHEEEEECCCCCCE
LLAHTLDDQAETVLLGLGRGSGARSIAGMRPHDPPWCRPLLGVRRSVPRAACHELGLNAW
EEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCC
QDPHNTDCRYTRTRLRLEVLPLLEDVLGGGVVEALARTATALREDTELIDTFAAQALPNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
CAGSGLHARALAALPDAVRRRVIRGWLLNGGATGLTDKQIRGVDTLVTAWRGQGGVAVGS
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHCCCCCEEECC
TLRGQRLIASRYDGVLTLHCAPV
CCCCHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002