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The map label for this gene is dacB [H]

Identifier: 221229492

GI number: 221229492

Start: 282172

End: 283557

Strand: Direct

Name: dacB [H]

Synonym: MLBr_00211

Alternate gene names: 221229492

Gene position: 282172-283557 (Clockwise)

Preceding gene: 221229490

Following gene: 221229493

Centisome position: 8.63

GC content: 64.07

Gene sequence:

>1386_bases
TTGAATCCTATTCGCTGGCAGAAATCTTACGTAGTTGTCGGATTGGCCATGCTGGCGTTCATCATCGCCGCGGTGAAGGC
GGCAACCGTCTTCACCACGGGCGGCCACAGCACGAATGGAGCACGGTCCGCGGTACCGCCTCCGCGGGCGTCTACGGTCA
AGCCGGCATTGGTTCCGGTAACCGAAACCGCCGTGGTACCCAGCGTTGGCGGACTGGCTGCCGCGCTGGCGTCGGCTTTG
GCAGATCCCAGTTTGGGTAGTTTTGGGGGACGGATCACCGATGCGATGACTGCCAAGGAACTCTGGCAGCAACAAGATGA
CATGCCGTTGGTGCCAGCGTCGGCCAATAAGCTCTTGACCGCAGCTGCGGCGCTGTTGACACTGGACCGCCAGACTCGGA
TCAGCACCCGGGTGGTGGCTGCAGGCCCGAATGCCCAAGGGCCTGTCGTTCTGGTGGGTGCTGGGGATCCGACCCTGTCG
GCCGCATCGCCCGACCAGAGCACCTGGTATAGGGGTGCGCCGCGGATCAGTGACCTTGTCGAACAGGTTCGTCGCAGCGG
CGTGACGCCTACGGCTGTTCAGGTGGATACCTCGCTGTTCACCGGTCCGACGATGGCGCAGGGCTGGGATCCTGCCGATG
TGGACAACGGCTACACTGCCCCGATCGAGTCGGCGATGATCGACGCTGGGCGTATCCAGCCGACAACCGTCAAATCACGA
CGGTCACGTACCCCAGCTCTGGACGCCGGGCGGGAATTGGCCAAGGCCCTGGGGGTCGCTCCCGATGCGGTTACAATCGT
CAAGGCATCGTCTGGGGCGCGGCAGCTGGCTGTGGTGCAGTCAGCGCCGCTGGTCCAGCGGTTATCCGAGATGATGGACA
ACTCCGACAACGTGCTGGCCGAATGCATCGGCCGCGAGGTAGCGGCCGCCATAAACCGGCCTTTGAGCTTTGCCGGCGCG
GTCGACGCGGTGACCAACCGGTTAGGTACAGCGCATATCGACACCACCGGCGCTGCTCTGGTGGATTCTAGCGGGTTGTC
GGTCAACAACCGCTTGACCGCCAAGACACTCGGCGGTGCGGTGCAGGCGGCGGCGGGGCCGGATCAACCGGTGTTGCGTG
CGCTGCTGGATCTGCTCCCGATCGCCGGCGGCAGCGGCACGTTATCGGACCGCTTTCTCAGTTCGGCGACCCACCAGGGT
CCGGCCGGCTGGTTACGTGCCAAGACCGGCTCGTTGACCGCTATTAATTCTTTGGTCGGGGTCGTCACTGACCGCAGCGG
TCGGGTGTTGACATTCGCGTTCATCTCCAACGACGCTGGCCCGACCGGTCGTGCCGCGATGGACGAGCTTGCTACTAGGC
TGTGGATGTGCGGGTGCGCTACGTGA

Upstream 100 bases:

>100_bases
AGGCCCGGGTAGATCGGTTACGTTGCGGAGTTCACCCTAGCCGACGACGTTGGTCTGAAGGCTGGCTCGGCACAATGGAG
CTAGACAGTCAGGAGGGCTA

Downstream 100 bases:

>100_bases
CCTCATCGTCGGAGTTCACGCTCGATACCGCGGTGGACTAGGGATTCGCGGCCAGCGTAGGAGAGCGGCTGGCACGTCCT
GGTCCGCCGGCTTCCGAATA

Product: putative carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MNPIRWQKSYVVVGLAMLAFIIAAVKAATVFTTGGHSTNGARSAVPPPRASTVKPALVPVTETAVVPSVGGLAAALASAL
ADPSLGSFGGRITDAMTAKELWQQQDDMPLVPASANKLLTAAAALLTLDRQTRISTRVVAAGPNAQGPVVLVGAGDPTLS
AASPDQSTWYRGAPRISDLVEQVRRSGVTPTAVQVDTSLFTGPTMAQGWDPADVDNGYTAPIESAMIDAGRIQPTTVKSR
RSRTPALDAGRELAKALGVAPDAVTIVKASSGARQLAVVQSAPLVQRLSEMMDNSDNVLAECIGREVAAAINRPLSFAGA
VDAVTNRLGTAHIDTTGAALVDSSGLSVNNRLTAKTLGGAVQAAAGPDQPVLRALLDLLPIAGGSGTLSDRFLSSATHQG
PAGWLRAKTGSLTAINSLVGVVTDRSGRVLTFAFISNDAGPTGRAAMDELATRLWMCGCAT

Sequences:

>Translated_461_residues
MNPIRWQKSYVVVGLAMLAFIIAAVKAATVFTTGGHSTNGARSAVPPPRASTVKPALVPVTETAVVPSVGGLAAALASAL
ADPSLGSFGGRITDAMTAKELWQQQDDMPLVPASANKLLTAAAALLTLDRQTRISTRVVAAGPNAQGPVVLVGAGDPTLS
AASPDQSTWYRGAPRISDLVEQVRRSGVTPTAVQVDTSLFTGPTMAQGWDPADVDNGYTAPIESAMIDAGRIQPTTVKSR
RSRTPALDAGRELAKALGVAPDAVTIVKASSGARQLAVVQSAPLVQRLSEMMDNSDNVLAECIGREVAAAINRPLSFAGA
VDAVTNRLGTAHIDTTGAALVDSSGLSVNNRLTAKTLGGAVQAAAGPDQPVLRALLDLLPIAGGSGTLSDRFLSSATHQG
PAGWLRAKTGSLTAINSLVGVVTDRSGRVLTFAFISNDAGPTGRAAMDELATRLWMCGCAT
>Mature_461_residues
MNPIRWQKSYVVVGLAMLAFIIAAVKAATVFTTGGHSTNGARSAVPPPRASTVKPALVPVTETAVVPSVGGLAAALASAL
ADPSLGSFGGRITDAMTAKELWQQQDDMPLVPASANKLLTAAAALLTLDRQTRISTRVVAAGPNAQGPVVLVGAGDPTLS
AASPDQSTWYRGAPRISDLVEQVRRSGVTPTAVQVDTSLFTGPTMAQGWDPADVDNGYTAPIESAMIDAGRIQPTTVKSR
RSRTPALDAGRELAKALGVAPDAVTIVKASSGARQLAVVQSAPLVQRLSEMMDNSDNVLAECIGREVAAAINRPLSFAGA
VDAVTNRLGTAHIDTTGAALVDSSGLSVNNRLTAKTLGGAVQAAAGPDQPVLRALLDLLPIAGGSGTLSDRFLSSATHQG
PAGWLRAKTGSLTAINSLVGVVTDRSGRVLTFAFISNDAGPTGRAAMDELATRLWMCGCAT

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=156, Percent_Identity=34.6153846153846, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47180; Mature: 47180

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIRWQKSYVVVGLAMLAFIIAAVKAATVFTTGGHSTNGARSAVPPPRASTVKPALVPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCEEEC
TETAVVPSVGGLAAALASALADPSLGSFGGRITDAMTAKELWQQQDDMPLVPASANKLLT
CHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AAAALLTLDRQTRISTRVVAAGPNAQGPVVLVGAGDPTLSAASPDQSTWYRGAPRISDLV
HHHHHHHHCCHHHCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
EQVRRSGVTPTAVQVDTSLFTGPTMAQGWDPADVDNGYTAPIESAMIDAGRIQPTTVKSR
HHHHHCCCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
RSRTPALDAGRELAKALGVAPDAVTIVKASSGARQLAVVQSAPLVQRLSEMMDNSDNVLA
HCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHCCHHHHHHHHHHCCCCHHHH
ECIGREVAAAINRPLSFAGAVDAVTNRLGTAHIDTTGAALVDSSGLSVNNRLTAKTLGGA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCEEECCCCCCCCEEEHHHHCCH
VQAAAGPDQPVLRALLDLLPIAGGSGTLSDRFLSSATHQGPAGWLRAKTGSLTAINSLVG
HCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
VVTDRSGRVLTFAFISNDAGPTGRAAMDELATRLWMCGCAT
HHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPIRWQKSYVVVGLAMLAFIIAAVKAATVFTTGGHSTNGARSAVPPPRASTVKPALVPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCEEEC
TETAVVPSVGGLAAALASALADPSLGSFGGRITDAMTAKELWQQQDDMPLVPASANKLLT
CHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AAAALLTLDRQTRISTRVVAAGPNAQGPVVLVGAGDPTLSAASPDQSTWYRGAPRISDLV
HHHHHHHHCCHHHCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
EQVRRSGVTPTAVQVDTSLFTGPTMAQGWDPADVDNGYTAPIESAMIDAGRIQPTTVKSR
HHHHHCCCCCCEEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
RSRTPALDAGRELAKALGVAPDAVTIVKASSGARQLAVVQSAPLVQRLSEMMDNSDNVLA
HCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHCCHHHHHHHHHHCCCCHHHH
ECIGREVAAAINRPLSFAGAVDAVTNRLGTAHIDTTGAALVDSSGLSVNNRLTAKTLGGA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCEEECCCCCCCCEEEHHHHCCH
VQAAAGPDQPVLRALLDLLPIAGGSGTLSDRFLSSATHQGPAGWLRAKTGSLTAINSLVG
HCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
VVTDRSGRVLTFAFISNDAGPTGRAAMDELATRLWMCGCAT
HHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]