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The map label for this gene is holB [H]

Identifier: 221229485

GI number: 221229485

Start: 270620

End: 271837

Strand: Direct

Name: holB [H]

Synonym: MLBr_00202

Alternate gene names: 221229485

Gene position: 270620-271837 (Clockwise)

Preceding gene: 221229483

Following gene: 221229487

Centisome position: 8.28

GC content: 64.78

Gene sequence:

>1218_bases
ATGTCCGGGGTGTTTACGCGGCTGGTGGGCCAGGATGCGGTGGAAGCTGAGCTGCTGGCCGCCGCTAAGGCCGCCCGTAG
TGACTTGGTTCACAGTCGGCCTGCTGACGGGACTATGACACATGCATGGCTCATCACCGGTCCGCCGGGCTCGGGACGCT
CGGTTGCGGCGGTGTGCTTCGCGGCTGCACTGCAATGCACTGCGGATTTTCAGGATGGCGGACCGGGGTGCGGGCGCTGC
CGGGCGTGTACGACGACGATGGCCGGCACCCACGCTGACGTTCGTCGGGTGATTCCCGATGGCCTGTCGATCGGCGTAGA
CGAGATGCGTGCCATCGTGCATGAGGCCTCGCGCCGTCCAAGCACCGGATATTGGCAGATCGTGCTGATCGAGGACGCCG
ACCGGTTGACCGAAGGAGCCGCTAACGCGCTGCTCAAGGTTGTTGAGGAACCGCCGTCGTCGACGGTGTTTTTGCTGTGC
GCACCGTCGGTGGCTCCAGAAGACATTGCGGTAACGTTACGGTCTCGCTGCCGGCATGTCGCGCTGGTGACCCCATCGCA
TGCGGCGATTGCTCGGGTATTGATTGACAGCGACGGGCTGGCCGCCGACACGGCGAACTGGGCGGCGTTGGTCAGCGGTG
GCCATGTGGGTCTGGCGCGTCGGTTAGCGACTGATCCAGAGGCCCAACAACGACGTGAGCGGGCGTTCGGGTTGATCCGC
GATGCCGTGGTGCCGTCGCGGGCGTATGCTGTGGCCGAGGAACTTGTGGCCGCCGCCGAAGCGGAGGCTCTGGTGCTGAC
CGCTGAGCGGGCCGAAGCCGAGACCGAAGAGCTGCGGACGGCTCTCGGGGCCGGCGGCACCGGAAAGGGCACCGCTGGGG
CATTGCGCAGCGTTACGGGTGCGATCAAGGATCTAGAGCGACGGCAGAAGTCCCGTCAGACTCGTGCTTCGCGTGATGCA
CTGGACCGAGTATTGATCGATCTGGCGACCTACTTTCGGGATGCCTTGCTCGTTTCGGCGAATGCGGGATCGGTGCAACC
CAATCATCCGGACATGGCTGACCGGGTGGCGGTGCTGGCCTGCCATGCTACACCGGAGCGGTTGTTGCGCTGCATCGAAG
CTGTGCTGGAGTGCCGCGAAGCGTTGGCGGTCAATGTCAAACCCAAGTTCGCGGTCGACGCTCTGGTCGCGACTATCGGC
CAGGAGCTGCGCTATTAA

Upstream 100 bases:

>100_bases
GTTATGGGACGGCTGTGCGGGCGGTTATAAGGTTACGGAAATGTTGCGACGCGGCCGCTGATCAGTGTCATGTCGGTCGG
TATCATTACTGTTGTCGTTG

Downstream 100 bases:

>100_bases
CTTCTCTTGTGGGGATGCACAGCTCTTGGGTTGGCCAGCGCACCTGCCGTAGACTCGTGCTGCCCGGCGCAAAGGTAGGC
CGCCTTAGCTCAATCGGTAG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSGVFTRLVGQDAVEAELLAAAKAARSDLVHSRPADGTMTHAWLITGPPGSGRSVAAVCFAAALQCTADFQDGGPGCGRC
RACTTTMAGTHADVRRVIPDGLSIGVDEMRAIVHEASRRPSTGYWQIVLIEDADRLTEGAANALLKVVEEPPSSTVFLLC
APSVAPEDIAVTLRSRCRHVALVTPSHAAIARVLIDSDGLAADTANWAALVSGGHVGLARRLATDPEAQQRRERAFGLIR
DAVVPSRAYAVAEELVAAAEAEALVLTAERAEAETEELRTALGAGGTGKGTAGALRSVTGAIKDLERRQKSRQTRASRDA
LDRVLIDLATYFRDALLVSANAGSVQPNHPDMADRVAVLACHATPERLLRCIEAVLECREALAVNVKPKFAVDALVATIG
QELRY

Sequences:

>Translated_405_residues
MSGVFTRLVGQDAVEAELLAAAKAARSDLVHSRPADGTMTHAWLITGPPGSGRSVAAVCFAAALQCTADFQDGGPGCGRC
RACTTTMAGTHADVRRVIPDGLSIGVDEMRAIVHEASRRPSTGYWQIVLIEDADRLTEGAANALLKVVEEPPSSTVFLLC
APSVAPEDIAVTLRSRCRHVALVTPSHAAIARVLIDSDGLAADTANWAALVSGGHVGLARRLATDPEAQQRRERAFGLIR
DAVVPSRAYAVAEELVAAAEAEALVLTAERAEAETEELRTALGAGGTGKGTAGALRSVTGAIKDLERRQKSRQTRASRDA
LDRVLIDLATYFRDALLVSANAGSVQPNHPDMADRVAVLACHATPERLLRCIEAVLECREALAVNVKPKFAVDALVATIG
QELRY
>Mature_404_residues
SGVFTRLVGQDAVEAELLAAAKAARSDLVHSRPADGTMTHAWLITGPPGSGRSVAAVCFAAALQCTADFQDGGPGCGRCR
ACTTTMAGTHADVRRVIPDGLSIGVDEMRAIVHEASRRPSTGYWQIVLIEDADRLTEGAANALLKVVEEPPSSTVFLLCA
PSVAPEDIAVTLRSRCRHVALVTPSHAAIARVLIDSDGLAADTANWAALVSGGHVGLARRLATDPEAQQRRERAFGLIRD
AVVPSRAYAVAEELVAAAEAEALVLTAERAEAETEELRTALGAGGTGKGTAGALRSVTGAIKDLERRQKSRQTRASRDAL
DRVLIDLATYFRDALLVSANAGSVQPNHPDMADRVAVLACHATPERLLRCIEAVLECREALAVNVKPKFAVDALVATIGQ
ELRY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=147, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787341, Length=151, Percent_Identity=37.7483443708609, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42715; Mature: 42584

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVFTRLVGQDAVEAELLAAAKAARSDLVHSRPADGTMTHAWLITGPPGSGRSVAAVCF
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTADFQDGGPGCGRCRACTTTMAGTHADVRRVIPDGLSIGVDEMRAIVHEASRRP
HHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
STGYWQIVLIEDADRLTEGAANALLKVVEEPPSSTVFLLCAPSVAPEDIAVTLRSRCRHV
CCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEE
ALVTPSHAAIARVLIDSDGLAADTANWAALVSGGHVGLARRLATDPEAQQRRERAFGLIR
EEECCCHHHHHHHHCCCCCCCCCCCCHHHEECCCCHHHHHHHCCCCHHHHHHHHHHHHHH
DAVVPSRAYAVAEELVAAAEAEALVLTAERAEAETEELRTALGAGGTGKGTAGALRSVTG
HHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIKDLERRQKSRQTRASRDALDRVLIDLATYFRDALLVSANAGSVQPNHPDMADRVAVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHEEEE
CHATPERLLRCIEAVLECREALAVNVKPKFAVDALVATIGQELRY
ECCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGVFTRLVGQDAVEAELLAAAKAARSDLVHSRPADGTMTHAWLITGPPGSGRSVAAVCF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
AAALQCTADFQDGGPGCGRCRACTTTMAGTHADVRRVIPDGLSIGVDEMRAIVHEASRRP
HHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
STGYWQIVLIEDADRLTEGAANALLKVVEEPPSSTVFLLCAPSVAPEDIAVTLRSRCRHV
CCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEE
ALVTPSHAAIARVLIDSDGLAADTANWAALVSGGHVGLARRLATDPEAQQRRERAFGLIR
EEECCCHHHHHHHHCCCCCCCCCCCCHHHEECCCCHHHHHHHCCCCHHHHHHHHHHHHHH
DAVVPSRAYAVAEELVAAAEAEALVLTAERAEAETEELRTALGAGGTGKGTAGALRSVTG
HHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AIKDLERRQKSRQTRASRDALDRVLIDLATYFRDALLVSANAGSVQPNHPDMADRVAVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHEEEE
CHATPERLLRCIEAVLECREALAVNVKPKFAVDALVATIGQELRY
ECCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]