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The map label for this gene is fadD29 [H]

Identifier: 221229441

GI number: 221229441

Start: 177197

End: 179239

Strand: Direct

Name: fadD29 [H]

Synonym: MLBr_00132

Alternate gene names: 221229441

Gene position: 177197-179239 (Clockwise)

Preceding gene: 221229440

Following gene: 221229442

Centisome position: 5.42

GC content: 56.09

Gene sequence:

>2043_bases
ATGCTGCGCAGGGTAATCAGCTTAGTTGTTTCGGCAAAAAGTCACGTCCTTTACCCCGCTATCGTGGGGCCTCATCAGAT
TTCGCGTCGCGGAGCCGGGCCAAGTATGAAGCGTCGACTCGGATTCGAGGACACGGTAGCGAAACGGTCTTACCTTCTTG
CCGTCAAGAGCCGAAGGATCATCATGAAAGCGAATGTGTCATTTCACGCTAGGGGTGAGGTGGCAACGCAACCTGCGCAG
GGCCCACCTGAGCAAACGGGACAACTGGTCAACGGGACTGCAACGCGATTCGCGATGGGCGAGTCGATGCTCACCGATCT
GTTGCAGAAAGCAGCCAGCCAGTTCCCCAATCGTGCGGCGTACAAGTTCATCGACTATGACGTGGATCCAGCGGGATTTA
CCGAAACTCTGACATGGTGGCAGGTCTATAGACGCTCGATGAGCGTGGCGTATGAGCTCAGGATCTACGCGTCGGACGGC
GATCGGGTCGCTATATTGGCCCCGCAGGGGCTTGAATACATAGTTGCCTTTATGGGAGCGCTTCAGGCCGGTCTCATTGC
TGTTCCTCTTCCAGCGCCTCAATTTGGCATTCATGACGAACGAATTTCTGCAGCATTGCGGGATTCTTTGCCCAGCGTAA
TTATTACGACCTCGGCGGCCATCGTTGAGGTCACAAAATATGCGCCACATGCACGCTCTGCGCAAGGCTCTGCGCCGGTC
GTCGTGGCGGTGGACGTGCTGGACCTGGACTCTCCACGAGAGCTCGATCCGAGTCGGTATGAGCGTCTGAATACGGCATA
TCTGCAGTACACATCGGGGTCCACCCGAGCACCGGCTGGTGTTATCGTTTCGCACAAGAACGTCATCACCAACTGTGTAC
AGTTAATGTCTGACTATTTGGGGGATATAGAGAAAGTTCCGTCAACTGCTGTCTCGTGGTTGCCGTTTTACCACGACATG
GGGTTGATCCTGGGCGTCATTCTACCGATGGTCAATCAAGACACTGCGGTGTTGTTGAGTCCGATGGCATTTTTGCAGAG
ACCGGCTCGCTGGATGCAATTACTGGGAAAATATCGCGGTCAGATTTCTTGTGCGCCGAATTTTGGTTTCGAATTAGCGG
TGCGCAGAACGTCGGATGACGATATGGCTGGACTTGATCTTGGACACGTGCGGGCGATTGCCACTGGGGCCGAACGCGTA
AACGCTGCGACGCTCCAGCGTTTCACCGAACGGTTTGCCCGCTTCAACCTAGATGAGACGGCAATTCGACCGTCATACGG
GCTTGCCGAGGCGACGGTGTACGTGGCTACAGCTGGGCCAGGACGCCCACCTAAAAGCATCTGTTTCGACTACCAGCATT
TGTCGGCCGGTAAGGCTAAGCGTAGCGCCAACGGCGCGGGTGATGGTGTGAACTTGGTCAGTTATGGCGCGCCGCGGGCG
TCGGTACTGCGGATAGTTGACCCCGATACCAGGATTGAGACCTCGGAGGGGACGGTCGGGGAAATCTGGGTGCAGGGGGA
CAACGTTGCTCGTGGTTATTGGCGCAACCCCGAAAACACTGAGCGGACGTTCGGTGGACAACTTGTCAATCCTTCGCCTG
GTACACCAGACGGCCCGTGGCTGCGCACCGAAGACCTCGGCGTCATTTTTGAAGGCGAACTGTTCATCACGGGTCGTATC
AAGGATCTCTTGGTCGTGGATGGGTCCAACCACTATCCGGATGACATCGAGGCGACGATCCAGGAAATCACCGGTGGTCG
GGTTGTGGCGATAGCTGTTCCGGATGATTGCACTGAGCAGTTGGTCACCATCATCGAACTCACAAAGTGGGGTTACGCGG
ATTGTGATGCGATGGAAAAGCTGTGCACGGTCAAACGTGAGGTCACCTCCGCGGTATCCAAGTCGCACCGGGTGCGTGTT
GCGGACGTCGTTATCGTGGCTCCGGGTTCAATTCCTGTCACCACCAGCGGCAAGGTTCGACGTGCAGCCTGTGTCGAGCG
TTATCTAAACCACGAATTCAACCGTCTAGACGCTACGGCATGA

Upstream 100 bases:

>100_bases
TCGGTGCGATCGACAGGTCAACTTCGAAGGCTAGATCAATAGGACGGGCCAAGTTGCCCCCAACCTTGTATCACACAAAC
CCGGGGTCCTGCTAGCGCTG

Downstream 100 bases:

>100_bases
ATGCCCACGTATGTGGGTTGTCCACTTTACCCCGGGATGAGCAATGACCAATCGTACTCTGTCTAGGGAAGAAATCCGAA
AGCTCGATCGCGACCTTCGG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MLRRVISLVVSAKSHVLYPAIVGPHQISRRGAGPSMKRRLGFEDTVAKRSYLLAVKSRRIIMKANVSFHARGEVATQPAQ
GPPEQTGQLVNGTATRFAMGESMLTDLLQKAASQFPNRAAYKFIDYDVDPAGFTETLTWWQVYRRSMSVAYELRIYASDG
DRVAILAPQGLEYIVAFMGALQAGLIAVPLPAPQFGIHDERISAALRDSLPSVIITTSAAIVEVTKYAPHARSAQGSAPV
VVAVDVLDLDSPRELDPSRYERLNTAYLQYTSGSTRAPAGVIVSHKNVITNCVQLMSDYLGDIEKVPSTAVSWLPFYHDM
GLILGVILPMVNQDTAVLLSPMAFLQRPARWMQLLGKYRGQISCAPNFGFELAVRRTSDDDMAGLDLGHVRAIATGAERV
NAATLQRFTERFARFNLDETAIRPSYGLAEATVYVATAGPGRPPKSICFDYQHLSAGKAKRSANGAGDGVNLVSYGAPRA
SVLRIVDPDTRIETSEGTVGEIWVQGDNVARGYWRNPENTERTFGGQLVNPSPGTPDGPWLRTEDLGVIFEGELFITGRI
KDLLVVDGSNHYPDDIEATIQEITGGRVVAIAVPDDCTEQLVTIIELTKWGYADCDAMEKLCTVKREVTSAVSKSHRVRV
ADVVIVAPGSIPVTTSGKVRRAACVERYLNHEFNRLDATA

Sequences:

>Translated_680_residues
MLRRVISLVVSAKSHVLYPAIVGPHQISRRGAGPSMKRRLGFEDTVAKRSYLLAVKSRRIIMKANVSFHARGEVATQPAQ
GPPEQTGQLVNGTATRFAMGESMLTDLLQKAASQFPNRAAYKFIDYDVDPAGFTETLTWWQVYRRSMSVAYELRIYASDG
DRVAILAPQGLEYIVAFMGALQAGLIAVPLPAPQFGIHDERISAALRDSLPSVIITTSAAIVEVTKYAPHARSAQGSAPV
VVAVDVLDLDSPRELDPSRYERLNTAYLQYTSGSTRAPAGVIVSHKNVITNCVQLMSDYLGDIEKVPSTAVSWLPFYHDM
GLILGVILPMVNQDTAVLLSPMAFLQRPARWMQLLGKYRGQISCAPNFGFELAVRRTSDDDMAGLDLGHVRAIATGAERV
NAATLQRFTERFARFNLDETAIRPSYGLAEATVYVATAGPGRPPKSICFDYQHLSAGKAKRSANGAGDGVNLVSYGAPRA
SVLRIVDPDTRIETSEGTVGEIWVQGDNVARGYWRNPENTERTFGGQLVNPSPGTPDGPWLRTEDLGVIFEGELFITGRI
KDLLVVDGSNHYPDDIEATIQEITGGRVVAIAVPDDCTEQLVTIIELTKWGYADCDAMEKLCTVKREVTSAVSKSHRVRV
ADVVIVAPGSIPVTTSGKVRRAACVERYLNHEFNRLDATA
>Mature_680_residues
MLRRVISLVVSAKSHVLYPAIVGPHQISRRGAGPSMKRRLGFEDTVAKRSYLLAVKSRRIIMKANVSFHARGEVATQPAQ
GPPEQTGQLVNGTATRFAMGESMLTDLLQKAASQFPNRAAYKFIDYDVDPAGFTETLTWWQVYRRSMSVAYELRIYASDG
DRVAILAPQGLEYIVAFMGALQAGLIAVPLPAPQFGIHDERISAALRDSLPSVIITTSAAIVEVTKYAPHARSAQGSAPV
VVAVDVLDLDSPRELDPSRYERLNTAYLQYTSGSTRAPAGVIVSHKNVITNCVQLMSDYLGDIEKVPSTAVSWLPFYHDM
GLILGVILPMVNQDTAVLLSPMAFLQRPARWMQLLGKYRGQISCAPNFGFELAVRRTSDDDMAGLDLGHVRAIATGAERV
NAATLQRFTERFARFNLDETAIRPSYGLAEATVYVATAGPGRPPKSICFDYQHLSAGKAKRSANGAGDGVNLVSYGAPRA
SVLRIVDPDTRIETSEGTVGEIWVQGDNVARGYWRNPENTERTFGGQLVNPSPGTPDGPWLRTEDLGVIFEGELFITGRI
KDLLVVDGSNHYPDDIEATIQEITGGRVVAIAVPDDCTEQLVTIIELTKWGYADCDAMEKLCTVKREVTSAVSKSHRVRV
ADVVIVAPGSIPVTTSGKVRRAACVERYLNHEFNRLDATA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=646, Percent_Identity=24.7678018575851, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI55749758, Length=657, Percent_Identity=24.2009132420091, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI225735629, Length=650, Percent_Identity=24.6153846153846, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI45827692, Length=650, Percent_Identity=24.6153846153846, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI225735625, Length=582, Percent_Identity=24.0549828178694, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI225735627, Length=476, Percent_Identity=25.4201680672269, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827696, Length=476, Percent_Identity=25.4201680672269, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827694, Length=496, Percent_Identity=25, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827698, Length=445, Percent_Identity=24.7191011235955, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=23.7076648841355, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=643, Percent_Identity=23.7947122861586, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=478, Percent_Identity=22.5941422594142, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=549, Percent_Identity=23.8615664845173, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859661, Length=460, Percent_Identity=26.7391304347826, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654656, Length=605, Percent_Identity=24.4628099173554, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 74148; Mature: 74148

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRVISLVVSAKSHVLYPAIVGPHQISRRGAGPSMKRRLGFEDTVAKRSYLLAVKSRRI
CHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCHHHHHHCCCHHHHHHHHEEEEEECCEE
IMKANVSFHARGEVATQPAQGPPEQTGQLVNGTATRFAMGESMLTDLLQKAASQFPNRAA
EEEECCEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
YKFIDYDVDPAGFTETLTWWQVYRRSMSVAYELRIYASDGDRVAILAPQGLEYIVAFMGA
EEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHH
LQAGLIAVPLPAPQFGIHDERISAALRDSLPSVIITTSAAIVEVTKYAPHARSAQGSAPV
HHCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHEEHHHCCCCCCCCCCCCCE
VVAVDVLDLDSPRELDPSRYERLNTAYLQYTSGSTRAPAGVIVSHKNVITNCVQLMSDYL
EEEEEEEECCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHH
GDIEKVPSTAVSWLPFYHDMGLILGVILPMVNQDTAVLLSPMAFLQRPARWMQLLGKYRG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCC
QISCAPNFGFELAVRRTSDDDMAGLDLGHVRAIATGAERVNAATLQRFTERFARFNLDET
EEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHH
AIRPSYGLAEATVYVATAGPGRPPKSICFDYQHLSAGKAKRSANGAGDGVNLVSYGAPRA
CCCCCCCCCEEEEEEEECCCCCCCHHHEECHHHHCCCCHHCCCCCCCCCCEEEECCCCCC
SVLRIVDPDTRIETSEGTVGEIWVQGDNVARGYWRNPENTERTFGGQLVNPSPGTPDGPW
EEEEEECCCCCEECCCCCEEEEEEECCCEECCCCCCCCCCCHHCCCEEECCCCCCCCCCE
LRTEDLGVIFEGELFITGRIKDLLVVDGSNHYPDDIEATIQEITGGRVVAIAVPDDCTEQ
EEECCCCEEEECCEEEEECCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHH
LVTIIELTKWGYADCDAMEKLCTVKREVTSAVSKSHRVRVADVVIVAPGSIPVTTSGKVR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCHH
RAACVERYLNHEFNRLDATA
HHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MLRRVISLVVSAKSHVLYPAIVGPHQISRRGAGPSMKRRLGFEDTVAKRSYLLAVKSRRI
CHHHHHHHHHHHCCCEEEEEEECCHHHHHCCCCHHHHHHCCCHHHHHHHHEEEEEECCEE
IMKANVSFHARGEVATQPAQGPPEQTGQLVNGTATRFAMGESMLTDLLQKAASQFPNRAA
EEEECCEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
YKFIDYDVDPAGFTETLTWWQVYRRSMSVAYELRIYASDGDRVAILAPQGLEYIVAFMGA
EEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEECCCCHHHHHHHHHH
LQAGLIAVPLPAPQFGIHDERISAALRDSLPSVIITTSAAIVEVTKYAPHARSAQGSAPV
HHCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHEEHHHCCCCCCCCCCCCCE
VVAVDVLDLDSPRELDPSRYERLNTAYLQYTSGSTRAPAGVIVSHKNVITNCVQLMSDYL
EEEEEEEECCCCCCCCHHHHHHCCEEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHH
GDIEKVPSTAVSWLPFYHDMGLILGVILPMVNQDTAVLLSPMAFLQRPARWMQLLGKYRG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCC
QISCAPNFGFELAVRRTSDDDMAGLDLGHVRAIATGAERVNAATLQRFTERFARFNLDET
EEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHH
AIRPSYGLAEATVYVATAGPGRPPKSICFDYQHLSAGKAKRSANGAGDGVNLVSYGAPRA
CCCCCCCCCEEEEEEEECCCCCCCHHHEECHHHHCCCCHHCCCCCCCCCCEEEECCCCCC
SVLRIVDPDTRIETSEGTVGEIWVQGDNVARGYWRNPENTERTFGGQLVNPSPGTPDGPW
EEEEEECCCCCEECCCCCEEEEEEECCCEECCCCCCCCCCCHHCCCEEECCCCCCCCCCE
LRTEDLGVIFEGELFITGRIKDLLVVDGSNHYPDDIEATIQEITGGRVVAIAVPDDCTEQ
EEECCCCEEEECCEEEEECCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHH
LVTIIELTKWGYADCDAMEKLCTVKREVTSAVSKSHRVRVADVVIVAPGSIPVTTSGKVR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCHH
RAACVERYLNHEFNRLDATA
HHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]