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The map label for this gene is recF

Identifier: 221229346

GI number: 221229346

Start: 3281

End: 4438

Strand: Direct

Name: recF

Synonym: MLBr_00003

Alternate gene names: 221229346

Gene position: 3281-4438 (Clockwise)

Preceding gene: 221229345

Following gene: 221229347

Centisome position: 0.1

GC content: 53.8

Gene sequence:

>1158_bases
GTGTACGTCCGGCATTTTGGACTGCGTGACTTTCGGTCCTGGGACCACGTTGACCTTGAGTTAAATCCAGGACGGACAGT
GTTTTTCGGTCCCAATGGTAACGGTAAAACAAATCTGATTGAGGCACTATGGTATTCCACCACTTTAAGTTCGCATCGGG
TGGGGACCGATATACCGTTAATCAGAGCGGGAACAATCCGAGCTATAGTTTCAACGATCGTGGTCAATGAAGGTAGGGAA
TGTGCGATTGATTTGGAGATTGCTGCGGGGCGGGCAAACAGGGCGCGATTGAATCGTTCACTTGTGCGCGGTATGCGTGA
GGTAGTTGGGGTATTGCGAGCGGTGCTGTTTGCCCCCGAAGATTTAGCGTTAGTTTGCGGAGATCCTGCCAATCGGCGTC
GTTACTTGGATGATTTGGCGACTGTGCGTCAGCCGGTGATTGCTGCCGTGCGCGCAGACTACGACAAGGTGCTGCGGCAA
CGCACTGCATTACTGAAGTCTTTAGCAGCTGCGCGGTATCGAAGTGACCAAGGTGTACTGGACACACTTGATGTATGGGA
TACTCGATTGGCTGAACATGGCGCCGAATTGATGGCTGCGCGTATCGATTTGGTAAATCAGTTGGCGCCAGAAGTGGAAA
AGGCGTATCAGTTGTTGGCACCGGGATCGCGCACTGCGTCGATCAGCTATCGTGCCAGTTTAGATATTGGAGGCATCGCA
GGTGTTGGGAGCAGTGACCGTGCCTTACTGCAAGCCGATCTGTTAGCTGGATTGTCGACGCGTCGGAACGTTGAGTTGGA
ACGCGGCATATGCTTAGTCGGTCCGCATCGCGATGAACTCGAGTTGCGACTTGGCGACCAACCTGCGAAAGGCTTTGCCA
GCCATGGGGAATCATGGTCGCTGGCGATAGCGTTGCGGTTGGCTGCCTATGAGTTGTTGCGTGCCGACGGTAACGAACCA
GTATTGTTGCTTGACGACGTATTTGCCGAACTCGATGCTGCCCGTTGTAGAGCATTGGCGACAGTGGCGGAATCAGCAGA
GCAGGTATTGGTAACCTCTGCGGCGCAGGAGGATATTCCGGTGGGTTGGGATGCTAAGTGGGTGACTGTCGATTTGCGTG
ACAGTGATTCGGGACGGGTATCGGTGGTGTATCCGTGA

Upstream 100 bases:

>100_bases
AACGTCTAATGATGATGTCCATCCGACGCATGATGGCCCCTTTCCCGCACTGCCAACTGACTATGTGTATTTACTGATGC
CAGTTCGGTTGCCTGGATAA

Downstream 100 bases:

>100_bases
TTGAATCCAATGAGTCATATAGCTACGGTGGCGATACCATCGAACCACTAGGCACGTTATCGGGCTTCGATTTGGTTAGG
CGCGCTCTTGAAGAAGCCCG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MYVRHFGLRDFRSWDHVDLELNPGRTVFFGPNGNGKTNLIEALWYSTTLSSHRVGTDIPLIRAGTIRAIVSTIVVNEGRE
CAIDLEIAAGRANRARLNRSLVRGMREVVGVLRAVLFAPEDLALVCGDPANRRRYLDDLATVRQPVIAAVRADYDKVLRQ
RTALLKSLAAARYRSDQGVLDTLDVWDTRLAEHGAELMAARIDLVNQLAPEVEKAYQLLAPGSRTASISYRASLDIGGIA
GVGSSDRALLQADLLAGLSTRRNVELERGICLVGPHRDELELRLGDQPAKGFASHGESWSLAIALRLAAYELLRADGNEP
VLLLDDVFAELDAARCRALATVAESAEQVLVTSAAQEDIPVGWDAKWVTVDLRDSDSGRVSVVYP

Sequences:

>Translated_385_residues
MYVRHFGLRDFRSWDHVDLELNPGRTVFFGPNGNGKTNLIEALWYSTTLSSHRVGTDIPLIRAGTIRAIVSTIVVNEGRE
CAIDLEIAAGRANRARLNRSLVRGMREVVGVLRAVLFAPEDLALVCGDPANRRRYLDDLATVRQPVIAAVRADYDKVLRQ
RTALLKSLAAARYRSDQGVLDTLDVWDTRLAEHGAELMAARIDLVNQLAPEVEKAYQLLAPGSRTASISYRASLDIGGIA
GVGSSDRALLQADLLAGLSTRRNVELERGICLVGPHRDELELRLGDQPAKGFASHGESWSLAIALRLAAYELLRADGNEP
VLLLDDVFAELDAARCRALATVAESAEQVLVTSAAQEDIPVGWDAKWVTVDLRDSDSGRVSVVYP
>Mature_385_residues
MYVRHFGLRDFRSWDHVDLELNPGRTVFFGPNGNGKTNLIEALWYSTTLSSHRVGTDIPLIRAGTIRAIVSTIVVNEGRE
CAIDLEIAAGRANRARLNRSLVRGMREVVGVLRAVLFAPEDLALVCGDPANRRRYLDDLATVRQPVIAAVRADYDKVLRQ
RTALLKSLAAARYRSDQGVLDTLDVWDTRLAEHGAELMAARIDLVNQLAPEVEKAYQLLAPGSRTASISYRASLDIGGIA
GVGSSDRALLQADLLAGLSTRRNVELERGICLVGPHRDELELRLGDQPAKGFASHGESWSLAIALRLAAYELLRADGNEP
VLLLDDVFAELDAARCRALATVAESAEQVLVTSAAQEDIPVGWDAKWVTVDLRDSDSGRVSVVYP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=386, Percent_Identity=25.6476683937824, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_MYCLB (B8ZTP0)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502762.1
- ProteinModelPortal:   B8ZTP0
- SMR:   B8ZTP0
- EnsemblBacteria:   EBMYCT00000086000
- GeneID:   7326182
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017189
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42067; Mature: 42067

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVRHFGLRDFRSWDHVDLELNPGRTVFFGPNGNGKTNLIEALWYSTTLSSHRVGTDIPL
CCEEECCCHHCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
IRAGTIRAIVSTIVVNEGRECAIDLEIAAGRANRARLNRSLVRGMREVVGVLRAVLFAPE
EECCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALVCGDPANRRRYLDDLATVRQPVIAAVRADYDKVLRQRTALLKSLAAARYRSDQGVL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTLDVWDTRLAEHGAELMAARIDLVNQLAPEVEKAYQLLAPGSRTASISYRASLDIGGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEE
GVGSSDRALLQADLLAGLSTRRNVELERGICLVGPHRDELELRLGDQPAKGFASHGESWS
CCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEEECCCCCCEEEEECCCCCCHHHHCCCCEE
LAIALRLAAYELLRADGNEPVLLLDDVFAELDAARCRALATVAESAEQVLVTSAAQEDIP
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VGWDAKWVTVDLRDSDSGRVSVVYP
CCCCCEEEEEEEECCCCCEEEEEEC
>Mature Secondary Structure
MYVRHFGLRDFRSWDHVDLELNPGRTVFFGPNGNGKTNLIEALWYSTTLSSHRVGTDIPL
CCEEECCCHHCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCE
IRAGTIRAIVSTIVVNEGRECAIDLEIAAGRANRARLNRSLVRGMREVVGVLRAVLFAPE
EECCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLALVCGDPANRRRYLDDLATVRQPVIAAVRADYDKVLRQRTALLKSLAAARYRSDQGVL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTLDVWDTRLAEHGAELMAARIDLVNQLAPEVEKAYQLLAPGSRTASISYRASLDIGGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEE
GVGSSDRALLQADLLAGLSTRRNVELERGICLVGPHRDELELRLGDQPAKGFASHGESWS
CCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEEECCCCCCEEEEECCCCCCHHHHCCCCEE
LAIALRLAAYELLRADGNEPVLLLDDVFAELDAARCRALATVAESAEQVLVTSAAQEDIP
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VGWDAKWVTVDLRDSDSGRVSVVYP
CCCCCEEEEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA