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The map label for this gene is dnaN

Identifier: 221229345

GI number: 221229345

Start: 2081

End: 3280

Strand: Direct

Name: dnaN

Synonym: MLBr_00002

Alternate gene names: 221229345

Gene position: 2081-3280 (Clockwise)

Preceding gene: 221229344

Following gene: 221229346

Centisome position: 0.06

GC content: 54.08

Gene sequence:

>1200_bases
ATGGACCTGGCCAAAACCAATGTTGGTTGCAGCGATTTAAAATTTTGTTTAGCACGTGAGTCGTTCGCCAGCGCGGTGTC
ATGGGTAGCGAAGTATCTCCCCACTAGACCAACGGTACCGGTGCTATCCGGCGTGCTGCTGACCGGTTCAGATAGCGGCC
TAACGATCTCCGGATTCGATTACGAAGTTTCCGCAGAGGTGCAGGTTGCTGCCGAGATAGCTTCTTCTGGAAGCGTTTTG
GTATCTGGGAGGTTGTTGTCAGATATTACTCGGGCGCTTCCGAACAAACCTGTTCACTTTTATGTGGACGGTAATCGGGT
TGCATTGACTTGCGGAAGTGCGAGGTTCTCGTTGCCGACGATGGCGGTTGAAGACTACCCTACACTGCCTACTTTGCCGG
ATGAAACTGGCACGTTGCCATCGGATGTGTTCGCTGAGGCGATAGGTCAGGTTGCTATTGCGGCCGGTCGTGACTATACC
TTGCCCATGCTTACCGGGATCCGGATCGAGATCTCGGGTGACACGGTAGTCTTGGCCGCCACCGATAGGTTCCGTTTGGC
GGTCCGCGAGTTAAAATGGTCGGTGTTGTCATCGGATTTCGAGGCATCGGTGCTGGTGCCGGCCAAAACTTTGGTGGAAG
TTGCTAAAGCCGGTACCGACGGCTCTGGTGTTTGTCTGTCATTGGGCGCTGGAGTCGGCGTTGGAAAAGATGGGCTTTTC
GGCATTAGCGGTGGCGGGAAGCGCAGTACTACTCGACTTCTTGACGCTGAGTTTCCGAAATTCAGGCAGTTATTGCCGGC
TGAGCACACCGCAGTGGCCACCATCGACGTGGCTGAGTTGACCGAGGCGATCAAACTGGTGGCGTTGGTAGCTGACCGTG
GCGCGCAAGTGCGCATGGAATTCGGTGACGGTATATTACGGCTTTCCGCCGGCGCCGATGATGTGGGCCGAGCCGAAGAA
GATCTTGCTGTTGCTTTTACTGGTGAACCGTTGACTATAGCGTTTAACCCGAATTATCTGACTGATGGACTTGCATCGGT
GCATTCAGAGCGGGTGTCATTTGGTTTCACGACACCGAGTAAGCCAGCATTGCTGCGTCCAACGTCTAATGATGATGTCC
ATCCGACGCATGATGGCCCCTTTCCCGCACTGCCAACTGACTATGTGTATTTACTGATGCCAGTTCGGTTGCCTGGATAA

Upstream 100 bases:

>100_bases
TGGTGCACTATGTGTCACCGACGTTCGTTACAGTGGGCTCACGCTAGCGGGGGCGGAGGCTAGCCGTTGGATTTCAACGA
TTTTTAGGTGAAGGGGCGCC

Downstream 100 bases:

>100_bases
GTGTACGTCCGGCATTTTGGACTGCGTGACTTTCGGTCCTGGGACCACGTTGACCTTGAGTTAAATCCAGGACGGACAGT
GTTTTTCGGTCCCAATGGTA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MDLAKTNVGCSDLKFCLARESFASAVSWVAKYLPTRPTVPVLSGVLLTGSDSGLTISGFDYEVSAEVQVAAEIASSGSVL
VSGRLLSDITRALPNKPVHFYVDGNRVALTCGSARFSLPTMAVEDYPTLPTLPDETGTLPSDVFAEAIGQVAIAAGRDYT
LPMLTGIRIEISGDTVVLAATDRFRLAVRELKWSVLSSDFEASVLVPAKTLVEVAKAGTDGSGVCLSLGAGVGVGKDGLF
GISGGGKRSTTRLLDAEFPKFRQLLPAEHTAVATIDVAELTEAIKLVALVADRGAQVRMEFGDGILRLSAGADDVGRAEE
DLAVAFTGEPLTIAFNPNYLTDGLASVHSERVSFGFTTPSKPALLRPTSNDDVHPTHDGPFPALPTDYVYLLMPVRLPG

Sequences:

>Translated_399_residues
MDLAKTNVGCSDLKFCLARESFASAVSWVAKYLPTRPTVPVLSGVLLTGSDSGLTISGFDYEVSAEVQVAAEIASSGSVL
VSGRLLSDITRALPNKPVHFYVDGNRVALTCGSARFSLPTMAVEDYPTLPTLPDETGTLPSDVFAEAIGQVAIAAGRDYT
LPMLTGIRIEISGDTVVLAATDRFRLAVRELKWSVLSSDFEASVLVPAKTLVEVAKAGTDGSGVCLSLGAGVGVGKDGLF
GISGGGKRSTTRLLDAEFPKFRQLLPAEHTAVATIDVAELTEAIKLVALVADRGAQVRMEFGDGILRLSAGADDVGRAEE
DLAVAFTGEPLTIAFNPNYLTDGLASVHSERVSFGFTTPSKPALLRPTSNDDVHPTHDGPFPALPTDYVYLLMPVRLPG
>Mature_399_residues
MDLAKTNVGCSDLKFCLARESFASAVSWVAKYLPTRPTVPVLSGVLLTGSDSGLTISGFDYEVSAEVQVAAEIASSGSVL
VSGRLLSDITRALPNKPVHFYVDGNRVALTCGSARFSLPTMAVEDYPTLPTLPDETGTLPSDVFAEAIGQVAIAAGRDYT
LPMLTGIRIEISGDTVVLAATDRFRLAVRELKWSVLSSDFEASVLVPAKTLVEVAKAGTDGSGVCLSLGAGVGVGKDGLF
GISGGGKRSTTRLLDAEFPKFRQLLPAEHTAVATIDVAELTEAIKLVALVADRGAQVRMEFGDGILRLSAGADDVGRAEE
DLAVAFTGEPLTIAFNPNYLTDGLASVHSERVSFGFTTPSKPALLRPTSNDDVHPTHDGPFPALPTDYVYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=349, Percent_Identity=28.080229226361, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_MYCLE (P46387)

Other databases:

- EMBL:   L39923
- EMBL:   Z70722
- EMBL:   AL583917
- PIR:   T10002
- RefSeq:   NP_301130.1
- ProteinModelPortal:   P46387
- SMR:   P46387
- EnsemblBacteria:   EBMYCT00000028433
- GeneID:   908144
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0002
- NMPDR:   fig|272631.1.peg.2
- Leproma:   ML0002
- GeneTree:   EBGT00050000015837
- HOGENOM:   HBG292442
- OMA:   DYNRVIP
- ProtClustDB:   PRK07761
- BioCyc:   MLEP272631:ML0002-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 41942; Mature: 41942

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAKTNVGCSDLKFCLARESFASAVSWVAKYLPTRPTVPVLSGVLLTGSDSGLTISGFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCEEEECCC
YEVSAEVQVAAEIASSGSVLVSGRLLSDITRALPNKPVHFYVDGNRVALTCGSARFSLPT
EEECCCEEEEEECCCCCCEEEECHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEECCCE
MAVEDYPTLPTLPDETGTLPSDVFAEAIGQVAIAAGRDYTLPMLTGIRIEISGDTVVLAA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEEEEEEEEEECCCEEEEEE
TDRFRLAVRELKWSVLSSDFEASVLVPAKTLVEVAKAGTDGSGVCLSLGAGVGVGKDGLF
CCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEE
GISGGGKRSTTRLLDAEFPKFRQLLPAEHTAVATIDVAELTEAIKLVALVADRGAQVRME
EECCCCCCCHHEECCCCCHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHEECCCCEEEEE
FGDGILRLSAGADDVGRAEEDLAVAFTGEPLTIAFNPNYLTDGLASVHSERVSFGFTTPS
CCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHEEECCCCCC
KPALLRPTSNDDVHPTHDGPFPALPTDYVYLLMPVRLPG
CCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MDLAKTNVGCSDLKFCLARESFASAVSWVAKYLPTRPTVPVLSGVLLTGSDSGLTISGFD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCEEEECCC
YEVSAEVQVAAEIASSGSVLVSGRLLSDITRALPNKPVHFYVDGNRVALTCGSARFSLPT
EEECCCEEEEEECCCCCCEEEECHHHHHHHHHCCCCCEEEEECCCEEEEEECCCEECCCE
MAVEDYPTLPTLPDETGTLPSDVFAEAIGQVAIAAGRDYTLPMLTGIRIEISGDTVVLAA
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCEEEEEEEEEEEECCCEEEEEE
TDRFRLAVRELKWSVLSSDFEASVLVPAKTLVEVAKAGTDGSGVCLSLGAGVGVGKDGLF
CCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEE
GISGGGKRSTTRLLDAEFPKFRQLLPAEHTAVATIDVAELTEAIKLVALVADRGAQVRME
EECCCCCCCHHEECCCCCHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHEECCCCEEEEE
FGDGILRLSAGADDVGRAEEDLAVAFTGEPLTIAFNPNYLTDGLASVHSERVSFGFTTPS
CCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHEEECCCCCC
KPALLRPTSNDDVHPTHDGPFPALPTDYVYLLMPVRLPG
CCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969512; 8733228; 11234002