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The map label for this gene is gcvT

Identifier: 220933176

GI number: 220933176

Start: 2562036

End: 2563109

Strand: Reverse

Name: gcvT

Synonym: Hore_23440

Alternate gene names: 220933176

Gene position: 2563109-2562036 (Counterclockwise)

Preceding gene: 220933177

Following gene: 220933175

Centisome position: 99.42

GC content: 41.81

Gene sequence:

>1074_bases
ATGAAAAAAACACCTTTGTTTGAGATCCATAAAGAGCTTGGAGCCCGGTTGATTGAGTTTCACGGGTGGAGTATGCCTGT
CCAGTATACCAGTATTATTGAAGAGCACAAAGCTGTCAGGAACCAGTGTGGCTTATTTGATGTGTCCCATATGGGTGAAA
TTTTGGTAGAAGGGCCCGGGGCATTAGAAAGCCTTCAGAAAATTGTTACCAACAATGTGGCCCGGTTAAAAAAAGGCCAG
GTTCTCTATACTCCCATGTGTAAAGATGATGGTGGTATTATTGATGACCTTTTAGTTTACTGTCTGGGTCAAGATAAGTA
TTTAATGGTTGTTAATGCCAGTAATATCGAAAAAGATTTTAACTGGGTCAGGGATAACAGCAATCAGAGAACAGAGGTTG
TTAATGAATCTGACAATTATGCTCTTTTAGCATTGCAGGGCCCGAATTCAAAGAAAATCCTGGAAAAAGTTTCTTCAGTA
AACCTTGATAGCTTGAAATTTTATAATTTTACAACAGGAACCCTGAAAGGAGCAGAAGTCCTGATTTCACGAACCGGATA
TACCGGGGAGCTGGGCTATGAGCTTTACCTGTCTCCAGATAAAGCGGTAGAGGTGTGGCAAGCCCTGATGGAAGCTGGAA
GTGATCTCGGACTTATACCGGCTGGATTAGGAGCCAGGGATACCCTCAGGCTGGAGAAGGGATACTGTCTTTACGGTAAT
GATATAGATGAAAATACCCACCCCCTTGAGGCAGGACTGGGATGGACTGTTAAGTTTGATAAGGCTAGCTTTATTGGGAA
AAGGGCCCTGTTAAAGTATAAGGAAGAGGGTTTAAGTAGAAAACTGGTTGGTTTTAAACTTAAAGGAAGGGGAATTCCCC
GCCATGGTTATCCTATAAAAGATAATGGTGACCAAATTGGGGTAGTTACCAGTGGTAGTATGTCGCCTACTCTCAGTGAA
GGTATCGGTATGGGCTATGTGAGATATGATAAAGCTACTCCGGGAGAATCTATAACTATTGTTGTAAGAAACAGGGCAAT
AACAGGGGAAGTTGTTAAATTACCTTTTATATAA

Upstream 100 bases:

>100_bases
GGGGGGACGAAACTCTGAACATAAGGACAGAGGGGGTCAGGGAAATCCTGGCCCCCTATTATTCTGTCCATAAATTCACA
AATTATAGGGGGTGGGCTTA

Downstream 100 bases:

>100_bases
ATTGATACCGGTGTTTTACCAGGTTTTGTGTTCAAGTTATTTAATTATAAATTATTCCCGGTTTATTTTTAGTCTATTAT
TACAGCAGTTATTTTTAAAA

Product: glycine cleavage system T protein

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKTPLFEIHKELGARLIEFHGWSMPVQYTSIIEEHKAVRNQCGLFDVSHMGEILVEGPGALESLQKIVTNNVARLKKGQ
VLYTPMCKDDGGIIDDLLVYCLGQDKYLMVVNASNIEKDFNWVRDNSNQRTEVVNESDNYALLALQGPNSKKILEKVSSV
NLDSLKFYNFTTGTLKGAEVLISRTGYTGELGYELYLSPDKAVEVWQALMEAGSDLGLIPAGLGARDTLRLEKGYCLYGN
DIDENTHPLEAGLGWTVKFDKASFIGKRALLKYKEEGLSRKLVGFKLKGRGIPRHGYPIKDNGDQIGVVTSGSMSPTLSE
GIGMGYVRYDKATPGESITIVVRNRAITGEVVKLPFI

Sequences:

>Translated_357_residues
MKKTPLFEIHKELGARLIEFHGWSMPVQYTSIIEEHKAVRNQCGLFDVSHMGEILVEGPGALESLQKIVTNNVARLKKGQ
VLYTPMCKDDGGIIDDLLVYCLGQDKYLMVVNASNIEKDFNWVRDNSNQRTEVVNESDNYALLALQGPNSKKILEKVSSV
NLDSLKFYNFTTGTLKGAEVLISRTGYTGELGYELYLSPDKAVEVWQALMEAGSDLGLIPAGLGARDTLRLEKGYCLYGN
DIDENTHPLEAGLGWTVKFDKASFIGKRALLKYKEEGLSRKLVGFKLKGRGIPRHGYPIKDNGDQIGVVTSGSMSPTLSE
GIGMGYVRYDKATPGESITIVVRNRAITGEVVKLPFI
>Mature_357_residues
MKKTPLFEIHKELGARLIEFHGWSMPVQYTSIIEEHKAVRNQCGLFDVSHMGEILVEGPGALESLQKIVTNNVARLKKGQ
VLYTPMCKDDGGIIDDLLVYCLGQDKYLMVVNASNIEKDFNWVRDNSNQRTEVVNESDNYALLALQGPNSKKILEKVSSV
NLDSLKFYNFTTGTLKGAEVLISRTGYTGELGYELYLSPDKAVEVWQALMEAGSDLGLIPAGLGARDTLRLEKGYCLYGN
DIDENTHPLEAGLGWTVKFDKASFIGKRALLKYKEEGLSRKLVGFKLKGRGIPRHGYPIKDNGDQIGVVTSGSMSPTLSE
GIGMGYVRYDKATPGESITIVVRNRAITGEVVKLPFI

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=366, Percent_Identity=35.5191256830601, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI257796258, Length=349, Percent_Identity=35.5300859598854, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI257796254, Length=362, Percent_Identity=32.8729281767956, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI257796256, Length=301, Percent_Identity=34.8837209302326, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI24797151, Length=336, Percent_Identity=27.9761904761905, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI197927446, Length=340, Percent_Identity=28.8235294117647, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI21361378, Length=340, Percent_Identity=28.8235294117647, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194306651, Length=379, Percent_Identity=26.3852242744063, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1789272, Length=368, Percent_Identity=39.4021739130435, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17560118, Length=372, Percent_Identity=36.2903225806452, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32563613, Length=315, Percent_Identity=29.8412698412698, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71994045, Length=315, Percent_Identity=26.031746031746, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994052, Length=315, Percent_Identity=26.031746031746, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320222, Length=381, Percent_Identity=33.5958005249344, Blast_Score=188, Evalue=9e-49,
Organism=Drosophila melanogaster, GI20129441, Length=374, Percent_Identity=36.096256684492, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI28571104, Length=339, Percent_Identity=28.9085545722714, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20130091, Length=322, Percent_Identity=28.8819875776398, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_HALOH (B8D1D7)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002510084.1
- ProteinModelPortal:   B8D1D7
- SMR:   B8D1D7
- GeneID:   7314227
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_23440
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 39458; Mature: 39458

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTPLFEIHKELGARLIEFHGWSMPVQYTSIIEEHKAVRNQCGLFDVSHMGEILVEGPG
CCCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEHHHCCCEEEECCC
ALESLQKIVTNNVARLKKGQVLYTPMCKDDGGIIDDLLVYCLGQDKYLMVVNASNIEKDF
HHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHH
NWVRDNSNQRTEVVNESDNYALLALQGPNSKKILEKVSSVNLDSLKFYNFTTGTLKGAEV
HHHCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEE
LISRTGYTGELGYELYLSPDKAVEVWQALMEAGSDLGLIPAGLGARDTLRLEKGYCLYGN
EEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCEEEECC
DIDENTHPLEAGLGWTVKFDKASFIGKRALLKYKEEGLSRKLVGFKLKGRGIPRHGYPIK
CCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCC
DNGDQIGVVTSGSMSPTLSEGIGMGYVRYDKATPGESITIVVRNRAITGEVVKLPFI
CCCCEEEEEECCCCCCCHHHCCCCCEEEECCCCCCCEEEEEEECCEECCCEEEECCC
>Mature Secondary Structure
MKKTPLFEIHKELGARLIEFHGWSMPVQYTSIIEEHKAVRNQCGLFDVSHMGEILVEGPG
CCCCCHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEHHHCCCEEEECCC
ALESLQKIVTNNVARLKKGQVLYTPMCKDDGGIIDDLLVYCLGQDKYLMVVNASNIEKDF
HHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHH
NWVRDNSNQRTEVVNESDNYALLALQGPNSKKILEKVSSVNLDSLKFYNFTTGTLKGAEV
HHHCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEE
LISRTGYTGELGYELYLSPDKAVEVWQALMEAGSDLGLIPAGLGARDTLRLEKGYCLYGN
EEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCEEEECC
DIDENTHPLEAGLGWTVKFDKASFIGKRALLKYKEEGLSRKLVGFKLKGRGIPRHGYPIK
CCCCCCCCHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCC
DNGDQIGVVTSGSMSPTLSEGIGMGYVRYDKATPGESITIVVRNRAITGEVVKLPFI
CCCCEEEEEECCCCCCCHHHCCCCCEEEECCCCCCCEEEEEEECCEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA