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The map label for this gene is gcvH

Identifier: 220933175

GI number: 220933175

Start: 2561444

End: 2561830

Strand: Reverse

Name: gcvH

Synonym: Hore_23430

Alternate gene names: 220933175

Gene position: 2561830-2561444 (Counterclockwise)

Preceding gene: 220933176

Following gene: 220933174

Centisome position: 99.37

GC content: 44.7

Gene sequence:

>387_bases
ATGAATGTACCGGAAAATTTAATGTATACCAAAAACCATGAATGGATCAAGGTCGATGGGGATACGGCCCTGGTAGGGGT
GACAGATTACGCCCAGAAGGAGCTCGGTGATATAGTATTTGTTGAACTACCGGAAGTCAGTGATGAATTTGCTCAATCTG
AAGGTTTTGCAGTTCTGGAATCGGTTAAAGCTGTGTCAGATGTTTACCTGCCTGTCGGGGGAGAGGTGCTTGAAGCCAAT
GAAGAATTACTGGAAAACCCCGAGCTGGTAAACCAGGAACCTTATGCTTCAGGATGGCTTGTTAAGATAAAGCTGGCCGA
CAAAAAGGAGCTTGAAGACCTTATGAGTAGTGAAGAATATGCCAGATACCTGGAGGAGGTAGAATAA

Upstream 100 bases:

>100_bases
GACTGACCCTGATTTTACTGGTAAAGGTTTTAAGTTGTAAGGATTTTAAGTTGTAAAATGGATAATATGAAAATAAAAAA
TCTATAAAGGGGGACTTAAA

Downstream 100 bases:

>100_bases
GTTGGATTATATCTCCAATACCCCTTCTGAGAAAGAGGCTATGCTAAAAAAAATAGGTGTAGAAAATGTTGAGGACCTCT
TTAAACCCATTCCTGATGAT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNVPENLMYTKNHEWIKVDGDTALVGVTDYAQKELGDIVFVELPEVSDEFAQSEGFAVLESVKAVSDVYLPVGGEVLEAN
EELLENPELVNQEPYASGWLVKIKLADKKELEDLMSSEEYARYLEEVE

Sequences:

>Translated_128_residues
MNVPENLMYTKNHEWIKVDGDTALVGVTDYAQKELGDIVFVELPEVSDEFAQSEGFAVLESVKAVSDVYLPVGGEVLEAN
EELLENPELVNQEPYASGWLVKIKLADKKELEDLMSSEEYARYLEEVE
>Mature_128_residues
MNVPENLMYTKNHEWIKVDGDTALVGVTDYAQKELGDIVFVELPEVSDEFAQSEGFAVLESVKAVSDVYLPVGGEVLEAN
EELLENPELVNQEPYASGWLVKIKLADKKELEDLMSSEEYARYLEEVE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=45, Blast_Score=122, Evalue=9e-29,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=44.1666666666667, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=50.7936507936508, Blast_Score=130, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=41.3223140495868, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=48, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=48.7179487179487, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_HALOH (B8D1D6)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002510083.1
- ProteinModelPortal:   B8D1D6
- SMR:   B8D1D6
- GeneID:   7314226
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_23430
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14414; Mature: 14414

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPENLMYTKNHEWIKVDGDTALVGVTDYAQKELGDIVFVELPEVSDEFAQSEGFAVLE
CCCCCCCEEECCCCEEEECCCEEEEECHHHHHHHHCCEEEEECCCCCHHHHHCCCHHHHH
SVKAVSDVYLPVGGEVLEANEELLENPELVNQEPYASGWLVKIKLADKKELEDLMSSEEY
HHHHHHHEECCCCCHHHHCCHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCHHHH
ARYLEEVE
HHHHHHCC
>Mature Secondary Structure
MNVPENLMYTKNHEWIKVDGDTALVGVTDYAQKELGDIVFVELPEVSDEFAQSEGFAVLE
CCCCCCCEEECCCCEEEECCCEEEEECHHHHHHHHCCEEEEECCCCCHHHHHCCCHHHHH
SVKAVSDVYLPVGGEVLEANEELLENPELVNQEPYASGWLVKIKLADKKELEDLMSSEEY
HHHHHHHEECCCCCHHHHCCHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCHHHH
ARYLEEVE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA