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The map label for this gene is ispE [H]

Identifier: 220932968

GI number: 220932968

Start: 2324919

End: 2325782

Strand: Reverse

Name: ispE [H]

Synonym: Hore_21360

Alternate gene names: 220932968

Gene position: 2325782-2324919 (Counterclockwise)

Preceding gene: 220932969

Following gene: 220932967

Centisome position: 90.21

GC content: 42.71

Gene sequence:

>864_bases
ATGGAAGAGGTTGTCATTAAGGCTCCGGCTAAAATCAATTTATGTCTTGATATAAAAGGCCTCAGGGAAGATGGTTACCA
TGAAGTAGACATGATTATGCAATCTATTGCCCTTTATGATAGACTTCATCTTCAAAAGAGCGGGGATGGAATTAAAATAT
CAACTAACTCTGACAGGGTTCCAACCGGCAGGGAGAATCTGGCCTATAAGGCTGCCGAACTCATACTCGATGAGGCCGGG
CTACATAACGGGGTCAATATACATATTGAAAAAAATATACCTGTCGCGGCTGGCCTGGCAGGAGGCAGTACAGATGCAGC
AGCTGTCCTTAAAGGTATTAACCAATTGTATGGGTTAAATTTCAGTTATAATAGACTGGTGTCAATGGCCAGAAAACTGG
GCTCGGATGTCCCCTTTTGTTTACAGGGGGGTACCGCCCGGGCAACCGGCAGGGGAGATTATTTAACTCAGCTTGACGAT
TTACAGAAAACTTACCTTGTGGTGGTAACCCCACCGGTGGCCTTGAGTACAGCTTATATTTATAGTCAGTATGATAAAAA
TCCATTTTCAGGCAGTATTCCGGTAACCCGTTTTGTAAATTTAATATCAAATAATAAAAAAATAACCTGGGAAGAGGGAT
GGTATAATGTTTTAGAACCTGTTTCGATAAAGGAGACCTTTGATATAAAGGAAATAAAGTCCCTTTTAGAGGACAGAGGG
GTCCGTCTGTCATTAATGTCCGGGAGTGGTCCCAGTGTTTTTGCCATAGTAGATAGTTGGGATAAGGGATGTGAAATCAA
AGAATCCTGGCCACGTCGGGGGGATTTTGTTCATCCCACCTGGACCCTAAAGAAGGAATTTTAA

Upstream 100 bases:

>100_bases
AAATATCCGGGGCCTTAGTCTGGGGATTTTATTTTTCTTTTATTAATGGTATAATTAAAATTAAGGAATTAGATCGAAAA
TATCGGTAAGGGGAAGATTT

Downstream 100 bases:

>100_bases
TTTCTTTAAGGAACAATATATATATTGGTTTTATTTGTCTATTTTTATTTGTTTGTCATTGATATATCTTATTAATTATT
TTCTTTTATTACTGGTTTTC

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MEEVVIKAPAKINLCLDIKGLREDGYHEVDMIMQSIALYDRLHLQKSGDGIKISTNSDRVPTGRENLAYKAAELILDEAG
LHNGVNIHIEKNIPVAAGLAGGSTDAAAVLKGINQLYGLNFSYNRLVSMARKLGSDVPFCLQGGTARATGRGDYLTQLDD
LQKTYLVVVTPPVALSTAYIYSQYDKNPFSGSIPVTRFVNLISNNKKITWEEGWYNVLEPVSIKETFDIKEIKSLLEDRG
VRLSLMSGSGPSVFAIVDSWDKGCEIKESWPRRGDFVHPTWTLKKEF

Sequences:

>Translated_287_residues
MEEVVIKAPAKINLCLDIKGLREDGYHEVDMIMQSIALYDRLHLQKSGDGIKISTNSDRVPTGRENLAYKAAELILDEAG
LHNGVNIHIEKNIPVAAGLAGGSTDAAAVLKGINQLYGLNFSYNRLVSMARKLGSDVPFCLQGGTARATGRGDYLTQLDD
LQKTYLVVVTPPVALSTAYIYSQYDKNPFSGSIPVTRFVNLISNNKKITWEEGWYNVLEPVSIKETFDIKEIKSLLEDRG
VRLSLMSGSGPSVFAIVDSWDKGCEIKESWPRRGDFVHPTWTLKKEF
>Mature_287_residues
MEEVVIKAPAKINLCLDIKGLREDGYHEVDMIMQSIALYDRLHLQKSGDGIKISTNSDRVPTGRENLAYKAAELILDEAG
LHNGVNIHIEKNIPVAAGLAGGSTDAAAVLKGINQLYGLNFSYNRLVSMARKLGSDVPFCLQGGTARATGRGDYLTQLDD
LQKTYLVVVTPPVALSTAYIYSQYDKNPFSGSIPVTRFVNLISNNKKITWEEGWYNVLEPVSIKETFDIKEIKSLLEDRG
VRLSLMSGSGPSVFAIVDSWDKGCEIKESWPRRGDFVHPTWTLKKEF

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=276, Percent_Identity=32.6086956521739, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31797; Mature: 31797

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVVIKAPAKINLCLDIKGLREDGYHEVDMIMQSIALYDRLHLQKSGDGIKISTNSDRV
CCCEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCC
PTGRENLAYKAAELILDEAGLHNGVNIHIEKNIPVAAGLAGGSTDAAAVLKGINQLYGLN
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCC
FSYNRLVSMARKLGSDVPFCLQGGTARATGRGDYLTQLDDLQKTYLVVVTPPVALSTAYI
CCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHH
YSQYDKNPFSGSIPVTRFVNLISNNKKITWEEGWYNVLEPVSIKETFDIKEIKSLLEDRG
HHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCCHHCCCHHHHHHHHHCCC
VRLSLMSGSGPSVFAIVDSWDKGCEIKESWPRRGDFVHPTWTLKKEF
CEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCEEEEEECC
>Mature Secondary Structure
MEEVVIKAPAKINLCLDIKGLREDGYHEVDMIMQSIALYDRLHLQKSGDGIKISTNSDRV
CCCEEEECCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCC
PTGRENLAYKAAELILDEAGLHNGVNIHIEKNIPVAAGLAGGSTDAAAVLKGINQLYGLN
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCC
FSYNRLVSMARKLGSDVPFCLQGGTARATGRGDYLTQLDDLQKTYLVVVTPPVALSTAYI
CCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHH
YSQYDKNPFSGSIPVTRFVNLISNNKKITWEEGWYNVLEPVSIKETFDIKEIKSLLEDRG
HHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCCHHCCCHHHHHHHHHCCC
VRLSLMSGSGPSVFAIVDSWDKGCEIKESWPRRGDFVHPTWTLKKEF
CEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA