Click here to switch to the map view.

The map label for this gene is kojP [H]

Identifier: 220932915

GI number: 220932915

Start: 2253970

End: 2256279

Strand: Reverse

Name: kojP [H]

Synonym: Hore_20830

Alternate gene names: 220932915

Gene position: 2256279-2253970 (Counterclockwise)

Preceding gene: 220932916

Following gene: 220932914

Centisome position: 87.52

GC content: 38.31

Gene sequence:

>2310_bases
ATGGAATTGAAAAACAACTTATACACAGAAACAGGATGGGAAATATTTGAAAGAGAATATGAAGATGAGCAGATGGTTAC
TACCGGTAGTAACTATCTTATAGGTAATGGTTATCTGGGATACAGGGGAACCTTTGCTGACTGGACTGCTAATGAGTATG
TAGCCTGTATAGTTACTGATACTTATGATATGGCTGATGGGAAATGGCGTGAATTATGTAATGCTCCCAATGGACTTTAT
ACCAGACTTGAAGTAGATGGGGAAAGAGTAACCATTAACAAAGGTGAGATCAGTAATTACAGAAGAGGGCTAAACCTGAG
AGAAGGGTTATACTCAGAGGAATTTACCTGGAAGGGTAATAAAGGGAATAAAGTCCATATTGAAACAGCCCGTTTTGCCA
GTTATCACAATCTTCATTTAATTCCCTTCCGTTATAGCTTTACCGTTGAAAAAGAAGCAGAAATAACCCTGGTTACCGGT
ATTGATGGAGTGGTGTGGAGTTTAAACGGTGAACATTTTAAAAAATATGACCTTAAAAAAATAGACAAATATCTGGCTAT
TGAAACTGTTACCGGAGAGAGGGGGATCCAGCTTGATGTAGTTGAAGGATATAGACTTGATGGTGCTACTCCCCGGTTTG
AAAAAATAATCAAAGAAGACAGGAAAATATTAAGGGAATTGAAATTCAAACTAAGTCCCGGTACCAGGGTTGTACTGGAA
AAAGTTATAAGTATATATTCGAGTAATGATATCAAGAATCCTTTAGACCAGGCCCAAAAAGATCTGGAAAAGGCCCTTCA
GTCTACTTTTACTGAGCTTAGAGAAAAAAACAGGGCTAACTGGATAAATTTCTGGAATAGAACCGATATAAAAATTAAGG
GAGATTTAAAGGGGCAAACAGCTCTTCGATTCAATTTATATCATAACAGAATTGCGACCCCTGCCCACAGTGATAGGTTA
CCCATCGGGGCCCGGGGGCTTTCATGTCAGGCTTATCAGGGGGCTGCCTTCTGGGATCAGGAAATCTTTAATATGCCTAT
GTTCCTTTACACCAGTCCTGAAACTGCCTGTAATATTTTGAAATACCGTTACCACACCCTTGATGGGGCCAGAAAAAAGG
CCAGAAAACTGGGTTACAGGGGTGCTTTTTATGCCTGGGTCAGTGGAAAAACTGGTGAAGAATTATGTCCCTCATATTTC
TTTAAAGATGTTATTAGTGGGCGGAAAATTCATAACCATTTTAATGACTGGCAGATCCATATTTCACCTGATATTGCCTA
TGCCATCTGGCATTATTTCCAGGTTACAGGTGACTGGAATTTTATTGTTAATTACGGAGCTGAAATGATTTTTGAAATAT
CCCGGTTCTTATATTCCCATGCTTATTTTAAAAAAGACAAAAACAGGTTTGAGTTTATCAGGTTGTTGGGGCCTGATGAA
TATCATGAAAATGTTGATAATAACGCCTTCACCAATTATCAGGCTAAATATACCCTGGAGCGAGCTCTTGATATTTATAA
TAAAATGAAAGACGAGAATAAAAAAGAGCTGGAATACTTAATGGAAAAAATAGAGTTAACGGAAAAGGAAATTGAAGACT
GGAAAGAGATGGCTGAACTTATTTACCTCCCTCAACCTGATGAAGAAACTTTATTAATAGAACAGTTTGATGGCTATTTT
GATCTAGAAGATACCCGCCCTGATGAGCTGGAGAAGAGATTGTTAGACCCTGGTGAGTACTGGGGTTGGCCCAATGGGGT
GGCTGTGGAGACCCAGGTTACCAAGCAGGCTGATGTTATCCAGTTATTCTGCCTCCATAACATTTTCAGTAAAGGAGTTA
TGAAGGCTAATTATGACTATTATGAACCCCGGACCCAGCATGGTTCTTCATTGAGCCCGTCAGCCTATTCCATTATAGCC
AGCCAGATAGGTTATGTAGAGGAAGCCTACAGTTATTTTATAAAATCCTGTACTATTGATCTTTATAACACAAACAAAGC
TGTCAGCGGGGGCACTTTTATTGGAGGAATTCATACAGCAGCCTGTGGAGCAGCCTGGCAGATGGTGGTATTTGGATTTG
CCGGGTTAAAAATAGATAATGATAATCTCAGTTTTAATCCAGTTTTACCCTCTAAATGGGATGAAATTAACTTCCCCCTT
CAATATAAAAATAACAGATTTAATGTAGTAATTACAAAAAGCCAGCTTAAAGTTAAATCATCAGAAGAAAATAAAGAAAC
ACAAAAAATTAGATATAAGGATGATTTAACTGAAATCAGACCAGGGGATGAAGTAATTATACCATTTTAA

Upstream 100 bases:

>100_bases
ATTTATAAACATGGCTAAATCCTTATCTGAACTATCCAGGCAACTTGATGATATTATAAAAAGCTTTCAATTGAAATAAA
AGGAGGGATCTAAATATACC

Downstream 100 bases:

>100_bases
AAGGGGCACCCGGTAAGGGTGCTCTTTTTAACTTACTTTAAAGGGAAAATGGTAAAAGAAAATTATTTCATATTTAATTA
AATTTCAGAGCCTATAATTG

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MELKNNLYTETGWEIFEREYEDEQMVTTGSNYLIGNGYLGYRGTFADWTANEYVACIVTDTYDMADGKWRELCNAPNGLY
TRLEVDGERVTINKGEISNYRRGLNLREGLYSEEFTWKGNKGNKVHIETARFASYHNLHLIPFRYSFTVEKEAEITLVTG
IDGVVWSLNGEHFKKYDLKKIDKYLAIETVTGERGIQLDVVEGYRLDGATPRFEKIIKEDRKILRELKFKLSPGTRVVLE
KVISIYSSNDIKNPLDQAQKDLEKALQSTFTELREKNRANWINFWNRTDIKIKGDLKGQTALRFNLYHNRIATPAHSDRL
PIGARGLSCQAYQGAAFWDQEIFNMPMFLYTSPETACNILKYRYHTLDGARKKARKLGYRGAFYAWVSGKTGEELCPSYF
FKDVISGRKIHNHFNDWQIHISPDIAYAIWHYFQVTGDWNFIVNYGAEMIFEISRFLYSHAYFKKDKNRFEFIRLLGPDE
YHENVDNNAFTNYQAKYTLERALDIYNKMKDENKKELEYLMEKIELTEKEIEDWKEMAELIYLPQPDEETLLIEQFDGYF
DLEDTRPDELEKRLLDPGEYWGWPNGVAVETQVTKQADVIQLFCLHNIFSKGVMKANYDYYEPRTQHGSSLSPSAYSIIA
SQIGYVEEAYSYFIKSCTIDLYNTNKAVSGGTFIGGIHTAACGAAWQMVVFGFAGLKIDNDNLSFNPVLPSKWDEINFPL
QYKNNRFNVVITKSQLKVKSSEENKETQKIRYKDDLTEIRPGDEVIIPF

Sequences:

>Translated_769_residues
MELKNNLYTETGWEIFEREYEDEQMVTTGSNYLIGNGYLGYRGTFADWTANEYVACIVTDTYDMADGKWRELCNAPNGLY
TRLEVDGERVTINKGEISNYRRGLNLREGLYSEEFTWKGNKGNKVHIETARFASYHNLHLIPFRYSFTVEKEAEITLVTG
IDGVVWSLNGEHFKKYDLKKIDKYLAIETVTGERGIQLDVVEGYRLDGATPRFEKIIKEDRKILRELKFKLSPGTRVVLE
KVISIYSSNDIKNPLDQAQKDLEKALQSTFTELREKNRANWINFWNRTDIKIKGDLKGQTALRFNLYHNRIATPAHSDRL
PIGARGLSCQAYQGAAFWDQEIFNMPMFLYTSPETACNILKYRYHTLDGARKKARKLGYRGAFYAWVSGKTGEELCPSYF
FKDVISGRKIHNHFNDWQIHISPDIAYAIWHYFQVTGDWNFIVNYGAEMIFEISRFLYSHAYFKKDKNRFEFIRLLGPDE
YHENVDNNAFTNYQAKYTLERALDIYNKMKDENKKELEYLMEKIELTEKEIEDWKEMAELIYLPQPDEETLLIEQFDGYF
DLEDTRPDELEKRLLDPGEYWGWPNGVAVETQVTKQADVIQLFCLHNIFSKGVMKANYDYYEPRTQHGSSLSPSAYSIIA
SQIGYVEEAYSYFIKSCTIDLYNTNKAVSGGTFIGGIHTAACGAAWQMVVFGFAGLKIDNDNLSFNPVLPSKWDEINFPL
QYKNNRFNVVITKSQLKVKSSEENKETQKIRYKDDLTEIRPGDEVIIPF
>Mature_769_residues
MELKNNLYTETGWEIFEREYEDEQMVTTGSNYLIGNGYLGYRGTFADWTANEYVACIVTDTYDMADGKWRELCNAPNGLY
TRLEVDGERVTINKGEISNYRRGLNLREGLYSEEFTWKGNKGNKVHIETARFASYHNLHLIPFRYSFTVEKEAEITLVTG
IDGVVWSLNGEHFKKYDLKKIDKYLAIETVTGERGIQLDVVEGYRLDGATPRFEKIIKEDRKILRELKFKLSPGTRVVLE
KVISIYSSNDIKNPLDQAQKDLEKALQSTFTELREKNRANWINFWNRTDIKIKGDLKGQTALRFNLYHNRIATPAHSDRL
PIGARGLSCQAYQGAAFWDQEIFNMPMFLYTSPETACNILKYRYHTLDGARKKARKLGYRGAFYAWVSGKTGEELCPSYF
FKDVISGRKIHNHFNDWQIHISPDIAYAIWHYFQVTGDWNFIVNYGAEMIFEISRFLYSHAYFKKDKNRFEFIRLLGPDE
YHENVDNNAFTNYQAKYTLERALDIYNKMKDENKKELEYLMEKIELTEKEIEDWKEMAELIYLPQPDEETLLIEQFDGYF
DLEDTRPDELEKRLLDPGEYWGWPNGVAVETQVTKQADVIQLFCLHNIFSKGVMKANYDYYEPRTQHGSSLSPSAYSIIA
SQIGYVEEAYSYFIKSCTIDLYNTNKAVSGGTFIGGIHTAACGAAWQMVVFGFAGLKIDNDNLSFNPVLPSKWDEINFPL
QYKNNRFNVVITKSQLKVKSSEENKETQKIRYKDDLTEIRPGDEVIIPF

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=506, Percent_Identity=24.5059288537549, Blast_Score=131, Evalue=3e-30,
Organism=Escherichia coli, GI1787575, Length=719, Percent_Identity=29.4853963838665, Blast_Score=332, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6325283, Length=707, Percent_Identity=22.4893917963225, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583760, Length=260, Percent_Identity=28.8461538461538, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 89437; Mature: 89437

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKNNLYTETGWEIFEREYEDEQMVTTGSNYLIGNGYLGYRGTFADWTANEYVACIVTD
CCCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEECCEECCCCCCCCCCCCCEEEEEEEC
TYDMADGKWRELCNAPNGLYTRLEVDGERVTINKGEISNYRRGLNLREGLYSEEFTWKGN
CCCCCCCHHHHHHCCCCCCEEEEEECCCEEEEECCCHHHHHCCCCHHHCCCCCCEEEECC
KGNKVHIETARFASYHNLHLIPFRYSFTVEKEAEITLVTGIDGVVWSLNGEHFKKYDLKK
CCCEEEEEEHHHCCCCCEEEEEEEEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHH
IDKYLAIETVTGERGIQLDVVEGYRLDGATPRFEKIIKEDRKILRELKFKLSPGTRVVLE
HHHEEEEEEECCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KVISIYSSNDIKNPLDQAQKDLEKALQSTFTELREKNRANWINFWNRTDIKIKGDLKGQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCE
ALRFNLYHNRIATPAHSDRLPIGARGLSCQAYQGAAFWDQEIFNMPMFLYTSPETACNIL
EEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCEEEEECCHHHHHHH
KYRYHTLDGARKKARKLGYRGAFYAWVSGKTGEELCPSYFFKDVISGRKIHNHFNDWQIH
HHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEE
ISPDIAYAIWHYFQVTGDWNFIVNYGAEMIFEISRFLYSHAYFKKDKNRFEFIRLLGPDE
ECCCHHEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
YHENVDNNAFTNYQAKYTLERALDIYNKMKDENKKELEYLMEKIELTEKEIEDWKEMAEL
HHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
IYLPQPDEETLLIEQFDGYFDLEDTRPDELEKRLLDPGEYWGWPNGVAVETQVTKQADVI
EECCCCCCCEEEEEECCCEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHH
QLFCLHNIFSKGVMKANYDYYEPRTQHGSSLSPSAYSIIASQIGYVEEAYSYFIKSCTID
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
LYNTNKAVSGGTFIGGIHTAACGAAWQMVVFGFAGLKIDNDNLSFNPVLPSKWDEINFPL
EEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCEEECCCCCEECCCCCCCCCCCCCCE
QYKNNRFNVVITKSQLKVKSSEENKETQKIRYKDDLTEIRPGDEVIIPF
EECCCEEEEEEEECEEEECCCCCCCHHHHEEECCCHHHCCCCCCEEECC
>Mature Secondary Structure
MELKNNLYTETGWEIFEREYEDEQMVTTGSNYLIGNGYLGYRGTFADWTANEYVACIVTD
CCCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEECCEECCCCCCCCCCCCCEEEEEEEC
TYDMADGKWRELCNAPNGLYTRLEVDGERVTINKGEISNYRRGLNLREGLYSEEFTWKGN
CCCCCCCHHHHHHCCCCCCEEEEEECCCEEEEECCCHHHHHCCCCHHHCCCCCCEEEECC
KGNKVHIETARFASYHNLHLIPFRYSFTVEKEAEITLVTGIDGVVWSLNGEHFKKYDLKK
CCCEEEEEEHHHCCCCCEEEEEEEEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHH
IDKYLAIETVTGERGIQLDVVEGYRLDGATPRFEKIIKEDRKILRELKFKLSPGTRVVLE
HHHEEEEEEECCCCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KVISIYSSNDIKNPLDQAQKDLEKALQSTFTELREKNRANWINFWNRTDIKIKGDLKGQT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCE
ALRFNLYHNRIATPAHSDRLPIGARGLSCQAYQGAAFWDQEIFNMPMFLYTSPETACNIL
EEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHCCCEEEEECCHHHHHHH
KYRYHTLDGARKKARKLGYRGAFYAWVSGKTGEELCPSYFFKDVISGRKIHNHFNDWQIH
HHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHCCCCCCEEE
ISPDIAYAIWHYFQVTGDWNFIVNYGAEMIFEISRFLYSHAYFKKDKNRFEFIRLLGPDE
ECCCHHEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
YHENVDNNAFTNYQAKYTLERALDIYNKMKDENKKELEYLMEKIELTEKEIEDWKEMAEL
HHHCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
IYLPQPDEETLLIEQFDGYFDLEDTRPDELEKRLLDPGEYWGWPNGVAVETQVTKQADVI
EECCCCCCCEEEEEECCCEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHH
QLFCLHNIFSKGVMKANYDYYEPRTQHGSSLSPSAYSIIASQIGYVEEAYSYFIKSCTID
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
LYNTNKAVSGGTFIGGIHTAACGAAWQMVVFGFAGLKIDNDNLSFNPVLPSKWDEINFPL
EEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCEEECCCCCEECCCCCCCCCCCCCCE
QYKNNRFNVVITKSQLKVKSSEENKETQKIRYKDDLTEIRPGDEVIIPF
EECCCEEEEEEEECEEEECCCCCCCHHHHEEECCCHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA