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The map label for this gene is hsdM [H]

Identifier: 220932854

GI number: 220932854

Start: 2180441

End: 2181895

Strand: Reverse

Name: hsdM [H]

Synonym: Hore_20220

Alternate gene names: 220932854

Gene position: 2181895-2180441 (Counterclockwise)

Preceding gene: 220932855

Following gene: 220932853

Centisome position: 84.63

GC content: 33.2

Gene sequence:

>1455_bases
ATGACTTTAACAAATTTTGTAAAAAATGTTCAAGATATAATGAGAATGGATTCTGGTGTTGATGGGGATGCTCAAAGAAT
TAGTCAACTCACCTGGATGCTATTTTTAAAAATTTTTGATACAAAAGAACAGGAAGAATGGGCTTTTGAAGATGACTATG
ATCTTAAAGGCCCTTTTGTACCTGAAAAATATCAATGGGGTAATTGGGCTGGTGTGCGCAAAGCTGATAGAATGAGTTCA
GATGAGCTTATAGAGTTTGTGGAAGAGTTATTTAAGGAACTAAAAAATCTCTCTGTTGATGAACATACTGACAGGAGAAA
AATATTGGTAAGAGATGTATTCGAAGACTCAAATAACTACATGAAGTCAGGTGTTTTACTATGGCAAGTAATTGATAAGA
TCAATGAAATTGATTTTGGGGACTATAAAGAAAGACATGCTTTTAATGATATATATGAAACAATACTTAAAGATCTTCAA
TCGGCAGGAAATGCAGGGGAATATTATACACCCCGGGCAGTTACTGATTTTGTTATTGATAGACTTAATCCACAGATTGG
TGAAAAGGTAGCAGATTTTGCCTGTGGTACAGGAGGTTTTTTAATATCAGCTTTAGAGCATATGAAAGCAAGTAAAGAAA
ATTTAACTACTGAGGAAAGTGAAACTATTAAAAATTCTTTACATGGTATTGAAAAAAAACCGATGCCTCATCTACTATGC
TGTACAAATATGATTCTCCATGACATAGATTTCCCGGATATTCTTCATCAAAATTCCTTGGCAACAAATGTTAGAGATTA
TCCAGAAGAAAAAAAATATGATGTTATTGCAATGAATCCTCCCTTTGGCGGTACTGAAGAAGAAGGTATTAAAATGAATT
TTCCTGCAGAGTATAGAACATCTGAAACAGCTGATCTGTTCATGACCCTTATTCTTCACCGGATTAAAAAAGGAGGCCGG
GTAGGTATTGTTTTACCTGATGGGTTTTTATTTGGTAATGATAATGCTAAAATAGTTATAAAGAAAAAATTATTAAACGA
GTTTAACCTTCATACTATTGTAAGGTTACCTAATGGTGTTTTTGCGCCTTATACAGATATTAATACCAATCTTTTATTTT
TTGATTACAATGGGACTGGTACAGAAGAAGTCTGGTATTATGAGCATCAACTACCTGAGGGTTATAAAAAGTATACCAAA
ACCAAACCAATTCGTATGGAAGAGTTTAAAGCAGAGCAAGAGTGGTGGGATAAAAGGGAAGAGAATGAATTTGCCTGGAA
GGTATCAAAAGAAGAAATAATTGAGCGTGATTATAATCTGGATTTTAAAAATCGCAATGTAGAAGAAGAGGATTTAGATC
ACCCCGAGGTTATTTTAAAAAAATATAATGAAGCTGTTAAAGAGGTAGAAGAAATTCAAGAAAAAATCATTGCTGAACTA
AGAAAGTTATTGTAG

Upstream 100 bases:

>100_bases
ATGCCAGGAAGAATATTTAAACTCTTTGGCGGAAAGAAAAAATATTTAGAAGCGATTAAGGAATTAGAAGCAGAAATATA
TAAAGGAGAGGTAAGTTGAA

Downstream 100 bases:

>100_bases
GTGGTAATATGAAAAAAATTATAGATAATTTTCAGTTAATCTTTGATTCCAGGGAAAAGATTCAGGAATTAAGAAATTTA
ATTTTAAATTTAGCTGTCAG

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTLTNFVKNVQDIMRMDSGVDGDAQRISQLTWMLFLKIFDTKEQEEWAFEDDYDLKGPFVPEKYQWGNWAGVRKADRMSS
DELIEFVEELFKELKNLSVDEHTDRRKILVRDVFEDSNNYMKSGVLLWQVIDKINEIDFGDYKERHAFNDIYETILKDLQ
SAGNAGEYYTPRAVTDFVIDRLNPQIGEKVADFACGTGGFLISALEHMKASKENLTTEESETIKNSLHGIEKKPMPHLLC
CTNMILHDIDFPDILHQNSLATNVRDYPEEKKYDVIAMNPPFGGTEEEGIKMNFPAEYRTSETADLFMTLILHRIKKGGR
VGIVLPDGFLFGNDNAKIVIKKKLLNEFNLHTIVRLPNGVFAPYTDINTNLLFFDYNGTGTEEVWYYEHQLPEGYKKYTK
TKPIRMEEFKAEQEWWDKREENEFAWKVSKEEIIERDYNLDFKNRNVEEEDLDHPEVILKKYNEAVKEVEEIQEKIIAEL
RKLL

Sequences:

>Translated_484_residues
MTLTNFVKNVQDIMRMDSGVDGDAQRISQLTWMLFLKIFDTKEQEEWAFEDDYDLKGPFVPEKYQWGNWAGVRKADRMSS
DELIEFVEELFKELKNLSVDEHTDRRKILVRDVFEDSNNYMKSGVLLWQVIDKINEIDFGDYKERHAFNDIYETILKDLQ
SAGNAGEYYTPRAVTDFVIDRLNPQIGEKVADFACGTGGFLISALEHMKASKENLTTEESETIKNSLHGIEKKPMPHLLC
CTNMILHDIDFPDILHQNSLATNVRDYPEEKKYDVIAMNPPFGGTEEEGIKMNFPAEYRTSETADLFMTLILHRIKKGGR
VGIVLPDGFLFGNDNAKIVIKKKLLNEFNLHTIVRLPNGVFAPYTDINTNLLFFDYNGTGTEEVWYYEHQLPEGYKKYTK
TKPIRMEEFKAEQEWWDKREENEFAWKVSKEEIIERDYNLDFKNRNVEEEDLDHPEVILKKYNEAVKEVEEIQEKIIAEL
RKLL
>Mature_483_residues
TLTNFVKNVQDIMRMDSGVDGDAQRISQLTWMLFLKIFDTKEQEEWAFEDDYDLKGPFVPEKYQWGNWAGVRKADRMSSD
ELIEFVEELFKELKNLSVDEHTDRRKILVRDVFEDSNNYMKSGVLLWQVIDKINEIDFGDYKERHAFNDIYETILKDLQS
AGNAGEYYTPRAVTDFVIDRLNPQIGEKVADFACGTGGFLISALEHMKASKENLTTEESETIKNSLHGIEKKPMPHLLCC
TNMILHDIDFPDILHQNSLATNVRDYPEEKKYDVIAMNPPFGGTEEEGIKMNFPAEYRTSETADLFMTLILHRIKKGGRV
GIVLPDGFLFGNDNAKIVIKKKLLNEFNLHTIVRLPNGVFAPYTDINTNLLFFDYNGTGTEEVWYYEHQLPEGYKKYTKT
KPIRMEEFKAEQEWWDKREENEFAWKVSKEEIIERDYNLDFKNRNVEEEDLDHPEVILKKYNEAVKEVEEIQEKIIAELR
KLL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=446, Percent_Identity=27.3542600896861, Blast_Score=138, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56618; Mature: 56486

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHC
>Mature Secondary Structure 
TLTNFVKNVQDIMRMDSGVDGDAQRISQLTWMLFLKIFDTKEQEEWAFEDDYDLKGPFV
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PEKYQWGNWAGVRKADRMSSDELIEFVEELFKELKNLSVDEHTDRRKILVRDVFEDSNNY
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
MKSGVLLWQVIDKINEIDFGDYKERHAFNDIYETILKDLQSAGNAGEYYTPRAVTDFVID
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
RLNPQIGEKVADFACGTGGFLISALEHMKASKENLTTEESETIKNSLHGIEKKPMPHLLC
HCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHH
CTNMILHDIDFPDILHQNSLATNVRDYPEEKKYDVIAMNPPFGGTEEEGIKMNFPAEYRT
HHHHHHHCCCCHHHHHCCHHHHCHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHCC
SETADLFMTLILHRIKKGGRVGIVLPDGFLFGNDNAKIVIKKKLLNEFNLHTIVRLPNGV
CHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCEEEEEHHHHHHCCCEEEEECCCCC
FAPYTDINTNLLFFDYNGTGTEEVWYYEHQLPEGYKKYTKTKPIRMEEFKAEQEWWDKRE
CCCCCCCCCEEEEEECCCCCCCCEEEEHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
ENEFAWKVSKEEIIERDYNLDFKNRNVEEEDLDHPEVILKKYNEAVKEVEEIQEKIIAEL
CCCCEEECCHHHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKLL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]