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The map label for this gene is purR [H]

Identifier: 220932814

GI number: 220932814

Start: 2131696

End: 2132706

Strand: Reverse

Name: purR [H]

Synonym: Hore_19800

Alternate gene names: 220932814

Gene position: 2132706-2131696 (Counterclockwise)

Preceding gene: 220932815

Following gene: 220932813

Centisome position: 82.72

GC content: 37.98

Gene sequence:

>1011_bases
ATGGCAACAATAAAAGACATTGCAAAACTTGCAGGTGTTTCAGTAACTACAGTATCAAAAGTTATCAATAATTATCCTGA
TATTAGTGATAAAACAAAGGAAAAGGTTATAAAAATCATGGAACAGCAAAATTACCGCCCTAATGCTATTGCCAGAAGCC
TGTCAACAAGCCGTTCGCGGTCAATAGGAGTATTTTTTACAGACCATTTAAATAGTGGGTTGAGGCACCCCTTTTTCAGA
GATATTATTTACGGGATCGAAAAGACATTTTTCCGAAAAGGGTATGACCTGATTTTATTTGCCCATCAATGGGGAGACAG
GTTCAGTTATACAGAAAAGTGTAAAAGTCGTCATGTTGATGGTGCCATCTTAATGGGGATGCCGAGGACTGATCCCAATC
TTGATAAATTAGTTAATTCAAATATACCAACAGTATTTATAGACCTCGATATAGTTGGCAAAAATGCTACGTATGTGATA
TCCGATAATGTTCAGGGGGCAAAACAGGCTGTGAATTATCTTTATTCCCTTGGCCATATAAAAATAGGTATGATTATGGG
ACAGCGGATTACTAAACCGGCACAGGATCGCCTGATTGGTTTTCAGGAAGAGTTAACGAATTTAGGTCTGGAGTATAACC
CGGAATGGATTATAGAGGCTGAATTCGGAGAAGAAGGCGGTTATCAAGCTATGAAAAGGATTATTACCCAGGAGATAAGA
CCATCTGCTGTGTTTTGCCAGGGTGATGAAATGGCCATTGGAGCTATTAACGCTATAAAAGAACATGGTTACAATGTACC
TCAAGATTTTTCTATAGTTGGCTTTGATAATATTGAAATAAGTAGTTATGTTTCCCCTGGTCTTACTACAATCCATCAGG
ATAAATTGACTATGGGAAAGAAGGCCGCCAGTATTCTTCTGGAAATGATTAATAACCCAAACAAAACCTTTTCTCCCGTA
GTGTTACCAACAAAATTAATCGAGAGGGAGTCATGTAGAAAGATTGGATAG

Upstream 100 bases:

>100_bases
TATATTAGAATTCCTGTAATAACAGCAATTATAATAAATGGAAACAAAAAATTTATGGATGTTATCTAAATTGCTTTAGT
AAATTAGGAGGTATTAATAA

Downstream 100 bases:

>100_bases
GTGTAGAGAGTGAAGGGATAGTCATGTATTAAAGGCTGGTCAACTTAAGATTATTAAATAAGGAGGAAAGATGATGCTTG
CCAAAATAAGGGGTTATTTT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATIKDIAKLAGVSVTTVSKVINNYPDISDKTKEKVIKIMEQQNYRPNAIARSLSTSRSRSIGVFFTDHLNSGLRHPFFR
DIIYGIEKTFFRKGYDLILFAHQWGDRFSYTEKCKSRHVDGAILMGMPRTDPNLDKLVNSNIPTVFIDLDIVGKNATYVI
SDNVQGAKQAVNYLYSLGHIKIGMIMGQRITKPAQDRLIGFQEELTNLGLEYNPEWIIEAEFGEEGGYQAMKRIITQEIR
PSAVFCQGDEMAIGAINAIKEHGYNVPQDFSIVGFDNIEISSYVSPGLTTIHQDKLTMGKKAASILLEMINNPNKTFSPV
VLPTKLIERESCRKIG

Sequences:

>Translated_336_residues
MATIKDIAKLAGVSVTTVSKVINNYPDISDKTKEKVIKIMEQQNYRPNAIARSLSTSRSRSIGVFFTDHLNSGLRHPFFR
DIIYGIEKTFFRKGYDLILFAHQWGDRFSYTEKCKSRHVDGAILMGMPRTDPNLDKLVNSNIPTVFIDLDIVGKNATYVI
SDNVQGAKQAVNYLYSLGHIKIGMIMGQRITKPAQDRLIGFQEELTNLGLEYNPEWIIEAEFGEEGGYQAMKRIITQEIR
PSAVFCQGDEMAIGAINAIKEHGYNVPQDFSIVGFDNIEISSYVSPGLTTIHQDKLTMGKKAASILLEMINNPNKTFSPV
VLPTKLIERESCRKIG
>Mature_335_residues
ATIKDIAKLAGVSVTTVSKVINNYPDISDKTKEKVIKIMEQQNYRPNAIARSLSTSRSRSIGVFFTDHLNSGLRHPFFRD
IIYGIEKTFFRKGYDLILFAHQWGDRFSYTEKCKSRHVDGAILMGMPRTDPNLDKLVNSNIPTVFIDLDIVGKNATYVIS
DNVQGAKQAVNYLYSLGHIKIGMIMGQRITKPAQDRLIGFQEELTNLGLEYNPEWIIEAEFGEEGGYQAMKRIITQEIRP
SAVFCQGDEMAIGAINAIKEHGYNVPQDFSIVGFDNIEISSYVSPGLTTIHQDKLTMGKKAASILLEMINNPNKTFSPVV
LPTKLIERESCRKIG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=37.5, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=33.0357142857143, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1790369, Length=350, Percent_Identity=32.8571428571429, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=31.9402985074627, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=32.3699421965318, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=27.9411764705882, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=27.4760383386581, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=30.0884955752212, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=27.5147928994083, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=28.4403669724771, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787906, Length=340, Percent_Identity=24.1176470588235, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=25.776397515528, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=329, Percent_Identity=23.7082066869301, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=24.773413897281, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790192, Length=213, Percent_Identity=29.1079812206573, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37718; Mature: 37587

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDIAKLAGVSVTTVSKVINNYPDISDKTKEKVIKIMEQQNYRPNAIARSLSTSRSR
CCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
SIGVFFTDHLNSGLRHPFFRDIIYGIEKTFFRKGYDLILFAHQWGDRFSYTEKCKSRHVD
EEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCC
GAILMGMPRTDPNLDKLVNSNIPTVFIDLDIVGKNATYVISDNVQGAKQAVNYLYSLGHI
CEEEECCCCCCCCHHHHHCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCE
KIGMIMGQRITKPAQDRLIGFQEELTNLGLEYNPEWIIEAEFGEEGGYQAMKRIITQEIR
EEEEHHCCCCCCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCC
PSAVFCQGDEMAIGAINAIKEHGYNVPQDFSIVGFDNIEISSYVSPGLTTIHQDKLTMGK
CCEEEECCCCEEHHHHHHHHHCCCCCCCCCEEEECCCEEEHHHCCCCCCEEEHHHHHHHH
KAASILLEMINNPNKTFSPVVLPTKLIERESCRKIG
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIKDIAKLAGVSVTTVSKVINNYPDISDKTKEKVIKIMEQQNYRPNAIARSLSTSRSR
CCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
SIGVFFTDHLNSGLRHPFFRDIIYGIEKTFFRKGYDLILFAHQWGDRFSYTEKCKSRHVD
EEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCCC
GAILMGMPRTDPNLDKLVNSNIPTVFIDLDIVGKNATYVISDNVQGAKQAVNYLYSLGHI
CEEEECCCCCCCCHHHHHCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCE
KIGMIMGQRITKPAQDRLIGFQEELTNLGLEYNPEWIIEAEFGEEGGYQAMKRIITQEIR
EEEEHHCCCCCCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCC
PSAVFCQGDEMAIGAINAIKEHGYNVPQDFSIVGFDNIEISSYVSPGLTTIHQDKLTMGK
CCEEEECCCCEEHHHHHHHHHCCCCCCCCCEEEECCCEEEHHHCCCCCCEEEHHHHHHHH
KAASILLEMINNPNKTFSPVVLPTKLIERESCRKIG
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA