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The map label for this gene is liaS [H]

Identifier: 220932803

GI number: 220932803

Start: 2116684

End: 2117742

Strand: Reverse

Name: liaS [H]

Synonym: Hore_19690

Alternate gene names: 220932803

Gene position: 2117742-2116684 (Counterclockwise)

Preceding gene: 220932804

Following gene: 220932802

Centisome position: 82.14

GC content: 38.34

Gene sequence:

>1059_bases
ATGAGAAAAAGTATTCTGGCTATAACTAAGAATTCTTTTAAAAGTGGTATTATTGGTATCGGGATATCACTTATGTTTTT
CTTTTCATTTTCTGGTTTTTCAAAAACTGACAACCCATTTTTGCTCTTTATAATCTATGTTATTTTCGGGTACATTGTTG
GTTTTTTAATTTCAATCAGCAGTCAGTCAGTGATTTATATTGCTGCAAATTTTTCTCTACCTGTAATTAGTAATGTACTG
GGGCGGAGTTTTATTACCTACCTGGCAAGTAGTCTGGTATTTTATTTGTGTGGACTTATTTTCTTTCCCTTAATTGGAGT
TCCTTTTAGTTTCAATTTATATATAAGTCTGGGGGTAGGGATTGGTTCGGTTATGATCTCCTTGTTTTTTTATGTGCTCG
AACAACAACAGGAGAAACTTGAACTTGAAAAGGAAAATAAAAAACTGGCCATTATTGAAGAAAGAAACAGGATTGCCCGG
GAACTCCATGATTCTGTATCCCAGAACCTTTTTGGAATCAACTTAAATCTTAATACCCTGGAGGTTTTAATTGATGAGGG
GGACCATGATAGGGTTCGAAAGACAGTTGAGGATTTGAAGAAGATGGTCCAGGAGGTCCAGACCGAAATGAGGCTTATGA
TCTATGAACTAAAACCGGTCCACCTCAGGGAGAAAGATTTTTTTGAAGCCCTGGAAAGCCTGGTTGAGTTGTACAGAAAC
CGTTATAATCTGAAAATCACCACACATCTTAATGGTAATGAAAACAGGGTTGAGAGTGAAGTCCAGCTTGCCTTATACCG
GGTAGCACAGGAGGCCCTAAATAATATTGTAAAACATGCTGATGCCGATAAGGTCAGCATAACCCTTGGTATAGGCAATA
ATGGAATTACAATGGCTATAAAGGATAATGGGAAAGGGTTTAATGTTGGCAGAGTTAAGGCAGGCAGTGGCCGGTTTGGG
ATTTCGGGAATGAAGGACAGGGTCAACGATGTTGGTGGTGACTTTGATATAGAGTCACAACCGGGACAGGGAACCACTGT
AAAAGTTTCTATTCATTAA

Upstream 100 bases:

>100_bases
AAACCCATGTCAGTAATATTTTAAAGAAACTGGATGTTAAAGACCGGACTCAGGCAGTTATTAAGGCTATAGAAAATAAT
CTGGTAGATAGGTGATATTA

Downstream 100 bases:

>100_bases
AATAAGGAGGATGAGGAAAATGTTAAAGAGATTTAATAGAAGCAACATTAAGAATATTTGGCCATTAAAGTTTATGGCAA
TGGTTATTATGGTGGGATTA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRKSILAITKNSFKSGIIGIGISLMFFFSFSGFSKTDNPFLLFIIYVIFGYIVGFLISISSQSVIYIAANFSLPVISNVL
GRSFITYLASSLVFYLCGLIFFPLIGVPFSFNLYISLGVGIGSVMISLFFYVLEQQQEKLELEKENKKLAIIEERNRIAR
ELHDSVSQNLFGINLNLNTLEVLIDEGDHDRVRKTVEDLKKMVQEVQTEMRLMIYELKPVHLREKDFFEALESLVELYRN
RYNLKITTHLNGNENRVESEVQLALYRVAQEALNNIVKHADADKVSITLGIGNNGITMAIKDNGKGFNVGRVKAGSGRFG
ISGMKDRVNDVGGDFDIESQPGQGTTVKVSIH

Sequences:

>Translated_352_residues
MRKSILAITKNSFKSGIIGIGISLMFFFSFSGFSKTDNPFLLFIIYVIFGYIVGFLISISSQSVIYIAANFSLPVISNVL
GRSFITYLASSLVFYLCGLIFFPLIGVPFSFNLYISLGVGIGSVMISLFFYVLEQQQEKLELEKENKKLAIIEERNRIAR
ELHDSVSQNLFGINLNLNTLEVLIDEGDHDRVRKTVEDLKKMVQEVQTEMRLMIYELKPVHLREKDFFEALESLVELYRN
RYNLKITTHLNGNENRVESEVQLALYRVAQEALNNIVKHADADKVSITLGIGNNGITMAIKDNGKGFNVGRVKAGSGRFG
ISGMKDRVNDVGGDFDIESQPGQGTTVKVSIH
>Mature_352_residues
MRKSILAITKNSFKSGIIGIGISLMFFFSFSGFSKTDNPFLLFIIYVIFGYIVGFLISISSQSVIYIAANFSLPVISNVL
GRSFITYLASSLVFYLCGLIFFPLIGVPFSFNLYISLGVGIGSVMISLFFYVLEQQQEKLELEKENKKLAIIEERNRIAR
ELHDSVSQNLFGINLNLNTLEVLIDEGDHDRVRKTVEDLKKMVQEVQTEMRLMIYELKPVHLREKDFFEALESLVELYRN
RYNLKITTHLNGNENRVESEVQLALYRVAQEALNNIVKHADADKVSITLGIGNNGITMAIKDNGKGFNVGRVKAGSGRFG
ISGMKDRVNDVGGDFDIESQPGQGTTVKVSIH

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=211, Percent_Identity=27.4881516587678, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787474, Length=210, Percent_Identity=26.1904761904762, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082311, Length=202, Percent_Identity=30.1980198019802, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39493; Mature: 39493

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSILAITKNSFKSGIIGIGISLMFFFSFSGFSKTDNPFLLFIIYVIFGYIVGFLISIS
CCCCEEEEECCCHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SQSVIYIAANFSLPVISNVLGRSFITYLASSLVFYLCGLIFFPLIGVPFSFNLYISLGVG
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCC
IGSVMISLFFYVLEQQQEKLELEKENKKLAIIEERNRIARELHDSVSQNLFGINLNLNTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCEE
EVLIDEGDHDRVRKTVEDLKKMVQEVQTEMRLMIYELKPVHLREKDFFEALESLVELYRN
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCHHHHHHHHHHHHHHHHC
RYNLKITTHLNGNENRVESEVQLALYRVAQEALNNIVKHADADKVSITLGIGNNGITMAI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEE
KDNGKGFNVGRVKAGSGRFGISGMKDRVNDVGGDFDIESQPGQGTTVKVSIH
ECCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRKSILAITKNSFKSGIIGIGISLMFFFSFSGFSKTDNPFLLFIIYVIFGYIVGFLISIS
CCCCEEEEECCCHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SQSVIYIAANFSLPVISNVLGRSFITYLASSLVFYLCGLIFFPLIGVPFSFNLYISLGVG
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCC
IGSVMISLFFYVLEQQQEKLELEKENKKLAIIEERNRIARELHDSVSQNLFGINLNLNTL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCEE
EVLIDEGDHDRVRKTVEDLKKMVQEVQTEMRLMIYELKPVHLREKDFFEALESLVELYRN
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCHHHHHHHHHHHHHHHHC
RYNLKITTHLNGNENRVESEVQLALYRVAQEALNNIVKHADADKVSITLGIGNNGITMAI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEE
KDNGKGFNVGRVKAGSGRFGISGMKDRVNDVGGDFDIESQPGQGTTVKVSIH
ECCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]