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The map label for this gene is metH [H]

Identifier: 220932793

GI number: 220932793

Start: 2103918

End: 2106377

Strand: Reverse

Name: metH [H]

Synonym: Hore_19590

Alternate gene names: 220932793

Gene position: 2106377-2103918 (Counterclockwise)

Preceding gene: 220932794

Following gene: 220932792

Centisome position: 81.7

GC content: 43.41

Gene sequence:

>2460_bases
GTGGCTAATGATTTTACCAGTACATTACAGGAAAAAATAATAATTGGGGATGGAGCTATGGGTACCATGCTTCAGGCCTG
TGGACTTTCATCCGGGCATGCCCCTGAAAGCTGGGTTATAAAAAAGCCGGATACCATATATAAAATCCATAAGGAATATG
TAGCAGCAGGTGCAGGGTTAATAGAAACCAATACCTTTGGGGCCAACAGGCTGAAACTTAAATCATTGGGGTTAGAAGAT
AAGATAGAAGAGATTAATGTAAAAGCCACAGGATTGGCCCGCAAAGCAGCGGGAAAGGTTTTTGTGGCAGGCTCGGTTGG
CCCTACTGGAAAATTAATGGAGCCCCATGGCGATCTTTCCTTCGACAGGGCCAGGGACGTTTTTAAAGAACAGATATCAT
ACCTGGTTCACGCCGGGGTAGATGTGGTTATTATCGAGACCATGAGTGATCTTAAAGAACTCCGGGCAGCGGTGGTAGCA
GCTAAAGAATTTAATGTACCGGTTATTGCCCAGATGACTTTTACAGAGAGGGGGATAACCTTGACGGGTTCTCCCCCTGA
GGTGGTTGCCATTGTTCTTGATAAAATGGGAGTAGATGTTATCGGTGTTAACTGTGTGGCCGGGATTGAAGAAGCCCTTA
TAGTTATAAAGAAAATGTCCCGGGTTACTTCAAAACCCCTGAGTGTTTTCCCAAATGCAGGGCTCCCTGAAAGCAAAGGT
GGCGAAACAGTTTATCCTCAAACCCCTGATGCCTTTACAGATAAAATACCAGAACTTTTAAAGTATAATGTAAAATTGAT
TGGGGGGTGCTGTGGGACCAATCCCCGGTTTATTAAAGGGATAAAACAGGTTGTTTATGAAGCTAAAGTAAGTAATAATA
CAAATACTGATGCAAAAGATGAGAATAAAAACCGGACATATTTAACTAGTAACCACACCTATTTACAGGCAAGTGAGGAT
AAACCGGTTTTTCTCATCGGGGAAAGGATTAATCCCACCGGGAGAAATGATTTAAAAGAGGCTTTAAAAGGAGAAAAATG
GTCTGTTTTAAGGAAAGAAGCCCGGGAACAGGTTATTGCCGGGGCCCACTTACTCGATGTTAATATCGGGATGGCCGGCA
TTGACCGGGAAAAGTGTATGAAAAAAGTTATCCAGGAATTACAGCTTGAGGTTGATGTTCCCCTGGTAATCGATACACCG
AGGACCGGAGTCCTGGAAACTGCTTTAAAGGAATATACCGGTCGGGCCTTGATCAACTCAGTAAATGGTAATCCTGACGT
TATGGATAAAGTATTTCCCCTGGCCTGTAGATATGGAGCAGCTGTTATTGGATTGACCCTTGATGATAGGGGGATTCCTT
CTGAGGTTGAGGGCAGGCTTGAGATTGCTAGACAGATTATAGCCAGGGCTAGAGAATATGGACTTGGTGAAGAAGATATT
ATTATTGACCCCATTGTCTTAACAGCCGGTTCTAACCAGTCAGAGGTTATGAAGGGGATTGAGACTTTAAAAAGGATCAA
GGAGGAACTGGGGGTTAAAACTACCATGGGTATCAGTAATGTTTCCCATGGGCTTCCGAACCGAAAATTAATTAATAGAA
CCTTTCTGGCTATGGCCCTGGGGGCAGGTCTTGACTTCCCCATTGTTGATCCCGTGGATGAGGATTTAATGGCCACAGTC
AGGGCAGCTAATCTTTTAACTGCCCGGGATAGAAACGGACAGAATTTTTTGAAGTGGTATGGGAATTTTCAGGGTAATAA
ATCCCTGGAAGAGGATCGGTTGGATAATAATAAAGAGACTAAATCATCCAGGGATAAGGAAAATGACCGTCTCCTGTCAT
CGATTGCGGATATAGTGGTTAGCGGAGATACTGACATTATAGAAGAACAGGTTGCCCGGGCCCTGGAGCTATATCAACCC
CGGGAAATAATTAATAGGGCCATGGTAAAGGGGCTTAAAAGGGTTGGTGACCTATATGACAGGGGAGAGTATTTTTTGCC
CCAGTTGATGCAGAGCTCCCGGGTTGTTAAAAAGGCCTTTGACCTTTTAAAGGAGAGGATGAACTCAAAAGGAAGAGAAA
TAAATAAAGCCGGCCGGATTCTGCTGGCTACTGTAGAAGGTGATATTCACGATATTGGCAAAAATATAGTTAAGGTAGTC
TTTAATAACTATGGCTTTGAGGTTGTAGATCTCGGGGTAAATGTTAAAAGTGATGATATTGTAGAAGCAGCCTTTGACCA
TAAAGTTGATATCGTGGGATTAAGTGCCTTAATGACCGTAACCATGGAGAAGATGAGGGAGACCGTCAATATCCTGAAAC
AGAAAGGGTTTAAGGGAGGAATAATCATTGGTGGTGCAGTTACCAGTGAGGATTTTGCCCGGGAGATAGGGGCTGACCTC
TATGCCGGTGATGCCCTCGATGGGGTAAGGAAAGTAAAAAAATATCTAAATTTAAATTAA

Upstream 100 bases:

>100_bases
AGAGTTTAACGGTTCGTTAGAACTTTTAAACCTCTTCTTCCAGCAAGAAGAGGTTTTTTAATGTCTAGGAAAAATACAAT
TGATTTTAGGGGGTTAAAAA

Downstream 100 bases:

>100_bases
TGTCCTATGAGCAGGGAGGAGAGAGTAGAGTTGGATTTTAGCAGGGTTTTAACGGAAAGGGAATTTATTGTTACAGCTGA
GCTAGATCCACCAAGAGGGG

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 819; Mature: 818

Protein sequence:

>819_residues
MANDFTSTLQEKIIIGDGAMGTMLQACGLSSGHAPESWVIKKPDTIYKIHKEYVAAGAGLIETNTFGANRLKLKSLGLED
KIEEINVKATGLARKAAGKVFVAGSVGPTGKLMEPHGDLSFDRARDVFKEQISYLVHAGVDVVIIETMSDLKELRAAVVA
AKEFNVPVIAQMTFTERGITLTGSPPEVVAIVLDKMGVDVIGVNCVAGIEEALIVIKKMSRVTSKPLSVFPNAGLPESKG
GETVYPQTPDAFTDKIPELLKYNVKLIGGCCGTNPRFIKGIKQVVYEAKVSNNTNTDAKDENKNRTYLTSNHTYLQASED
KPVFLIGERINPTGRNDLKEALKGEKWSVLRKEAREQVIAGAHLLDVNIGMAGIDREKCMKKVIQELQLEVDVPLVIDTP
RTGVLETALKEYTGRALINSVNGNPDVMDKVFPLACRYGAAVIGLTLDDRGIPSEVEGRLEIARQIIARAREYGLGEEDI
IIDPIVLTAGSNQSEVMKGIETLKRIKEELGVKTTMGISNVSHGLPNRKLINRTFLAMALGAGLDFPIVDPVDEDLMATV
RAANLLTARDRNGQNFLKWYGNFQGNKSLEEDRLDNNKETKSSRDKENDRLLSSIADIVVSGDTDIIEEQVARALELYQP
REIINRAMVKGLKRVGDLYDRGEYFLPQLMQSSRVVKKAFDLLKERMNSKGREINKAGRILLATVEGDIHDIGKNIVKVV
FNNYGFEVVDLGVNVKSDDIVEAAFDHKVDIVGLSALMTVTMEKMRETVNILKQKGFKGGIIIGGAVTSEDFAREIGADL
YAGDALDGVRKVKKYLNLN

Sequences:

>Translated_819_residues
MANDFTSTLQEKIIIGDGAMGTMLQACGLSSGHAPESWVIKKPDTIYKIHKEYVAAGAGLIETNTFGANRLKLKSLGLED
KIEEINVKATGLARKAAGKVFVAGSVGPTGKLMEPHGDLSFDRARDVFKEQISYLVHAGVDVVIIETMSDLKELRAAVVA
AKEFNVPVIAQMTFTERGITLTGSPPEVVAIVLDKMGVDVIGVNCVAGIEEALIVIKKMSRVTSKPLSVFPNAGLPESKG
GETVYPQTPDAFTDKIPELLKYNVKLIGGCCGTNPRFIKGIKQVVYEAKVSNNTNTDAKDENKNRTYLTSNHTYLQASED
KPVFLIGERINPTGRNDLKEALKGEKWSVLRKEAREQVIAGAHLLDVNIGMAGIDREKCMKKVIQELQLEVDVPLVIDTP
RTGVLETALKEYTGRALINSVNGNPDVMDKVFPLACRYGAAVIGLTLDDRGIPSEVEGRLEIARQIIARAREYGLGEEDI
IIDPIVLTAGSNQSEVMKGIETLKRIKEELGVKTTMGISNVSHGLPNRKLINRTFLAMALGAGLDFPIVDPVDEDLMATV
RAANLLTARDRNGQNFLKWYGNFQGNKSLEEDRLDNNKETKSSRDKENDRLLSSIADIVVSGDTDIIEEQVARALELYQP
REIINRAMVKGLKRVGDLYDRGEYFLPQLMQSSRVVKKAFDLLKERMNSKGREINKAGRILLATVEGDIHDIGKNIVKVV
FNNYGFEVVDLGVNVKSDDIVEAAFDHKVDIVGLSALMTVTMEKMRETVNILKQKGFKGGIIIGGAVTSEDFAREIGADL
YAGDALDGVRKVKKYLNLN
>Mature_818_residues
ANDFTSTLQEKIIIGDGAMGTMLQACGLSSGHAPESWVIKKPDTIYKIHKEYVAAGAGLIETNTFGANRLKLKSLGLEDK
IEEINVKATGLARKAAGKVFVAGSVGPTGKLMEPHGDLSFDRARDVFKEQISYLVHAGVDVVIIETMSDLKELRAAVVAA
KEFNVPVIAQMTFTERGITLTGSPPEVVAIVLDKMGVDVIGVNCVAGIEEALIVIKKMSRVTSKPLSVFPNAGLPESKGG
ETVYPQTPDAFTDKIPELLKYNVKLIGGCCGTNPRFIKGIKQVVYEAKVSNNTNTDAKDENKNRTYLTSNHTYLQASEDK
PVFLIGERINPTGRNDLKEALKGEKWSVLRKEAREQVIAGAHLLDVNIGMAGIDREKCMKKVIQELQLEVDVPLVIDTPR
TGVLETALKEYTGRALINSVNGNPDVMDKVFPLACRYGAAVIGLTLDDRGIPSEVEGRLEIARQIIARAREYGLGEEDII
IDPIVLTAGSNQSEVMKGIETLKRIKEELGVKTTMGISNVSHGLPNRKLINRTFLAMALGAGLDFPIVDPVDEDLMATVR
AANLLTARDRNGQNFLKWYGNFQGNKSLEEDRLDNNKETKSSRDKENDRLLSSIADIVVSGDTDIIEEQVARALELYQPR
EIINRAMVKGLKRVGDLYDRGEYFLPQLMQSSRVVKKAFDLLKERMNSKGREINKAGRILLATVEGDIHDIGKNIVKVVF
NNYGFEVVDLGVNVKSDDIVEAAFDHKVDIVGLSALMTVTMEKMRETVNILKQKGFKGGIIIGGAVTSEDFAREIGADLY
AGDALDGVRKVKKYLNLN

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=865, Percent_Identity=30.2890173410405, Blast_Score=331, Evalue=2e-90,
Organism=Homo sapiens, GI13162290, Length=297, Percent_Identity=28.6195286195286, Blast_Score=100, Evalue=9e-21,
Organism=Homo sapiens, GI157266337, Length=301, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-19,
Organism=Escherichia coli, GI1790450, Length=847, Percent_Identity=30.814639905549, Blast_Score=328, Evalue=8e-91,
Organism=Escherichia coli, GI1786456, Length=297, Percent_Identity=28.2828282828283, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=853, Percent_Identity=29.7772567409144, Blast_Score=311, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6322966, Length=310, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 89714; Mature: 89582

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDFTSTLQEKIIIGDGAMGTMLQACGLSSGHAPESWVIKKPDTIYKIHKEYVAAGAGL
CCCHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCE
IETNTFGANRLKLKSLGLEDKIEEINVKATGLARKAAGKVFVAGSVGPTGKLMEPHGDLS
EEECCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC
FDRARDVFKEQISYLVHAGVDVVIIETMSDLKELRAAVVAAKEFNVPVIAQMTFTERGIT
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
LTGSPPEVVAIVLDKMGVDVIGVNCVAGIEEALIVIKKMSRVTSKPLSVFPNAGLPESKG
EECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
GETVYPQTPDAFTDKIPELLKYNVKLIGGCCGTNPRFIKGIKQVVYEAKVSNNTNTDAKD
CCCCCCCCCCHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
ENKNRTYLTSNHTYLQASEDKPVFLIGERINPTGRNDLKEALKGEKWSVLRKEAREQVIA
CCCCCCEEECCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
GAHLLDVNIGMAGIDREKCMKKVIQELQLEVDVPLVIDTPRTGVLETALKEYTGRALINS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHCC
VNGNPDVMDKVFPLACRYGAAVIGLTLDDRGIPSEVEGRLEIARQIIARAREYGLGEEDI
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIDPIVLTAGSNQSEVMKGIETLKRIKEELGVKTTMGISNVSHGLPNRKLINRTFLAMAL
EECCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHCCCCCHHHHHHHHHHHHH
GAGLDFPIVDPVDEDLMATVRAANLLTARDRNGQNFLKWYGNFQGNKSLEEDRLDNNKET
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCCHH
KSSRDKENDRLLSSIADIVVSGDTDIIEEQVARALELYQPREIINRAMVKGLKRVGDLYD
HHCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RGEYFLPQLMQSSRVVKKAFDLLKERMNSKGREINKAGRILLATVEGDIHDIGKNIVKVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHH
FNNYGFEVVDLGVNVKSDDIVEAAFDHKVDIVGLSALMTVTMEKMRETVNILKQKGFKGG
HHCCCCEEEEECCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IIIGGAVTSEDFAREIGADLYAGDALDGVRKVKKYLNLN
EEEECCCCCHHHHHHHCCCHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANDFTSTLQEKIIIGDGAMGTMLQACGLSSGHAPESWVIKKPDTIYKIHKEYVAAGAGL
CCHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCE
IETNTFGANRLKLKSLGLEDKIEEINVKATGLARKAAGKVFVAGSVGPTGKLMEPHGDLS
EEECCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCC
FDRARDVFKEQISYLVHAGVDVVIIETMSDLKELRAAVVAAKEFNVPVIAQMTFTERGIT
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
LTGSPPEVVAIVLDKMGVDVIGVNCVAGIEEALIVIKKMSRVTSKPLSVFPNAGLPESKG
EECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCC
GETVYPQTPDAFTDKIPELLKYNVKLIGGCCGTNPRFIKGIKQVVYEAKVSNNTNTDAKD
CCCCCCCCCCHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
ENKNRTYLTSNHTYLQASEDKPVFLIGERINPTGRNDLKEALKGEKWSVLRKEAREQVIA
CCCCCCEEECCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
GAHLLDVNIGMAGIDREKCMKKVIQELQLEVDVPLVIDTPRTGVLETALKEYTGRALINS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCHHHHCC
VNGNPDVMDKVFPLACRYGAAVIGLTLDDRGIPSEVEGRLEIARQIIARAREYGLGEEDI
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
IIDPIVLTAGSNQSEVMKGIETLKRIKEELGVKTTMGISNVSHGLPNRKLINRTFLAMAL
EECCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHCCCCCHHHHHHHHHHHHH
GAGLDFPIVDPVDEDLMATVRAANLLTARDRNGQNFLKWYGNFQGNKSLEEDRLDNNKET
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHCCCCCHH
KSSRDKENDRLLSSIADIVVSGDTDIIEEQVARALELYQPREIINRAMVKGLKRVGDLYD
HHCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RGEYFLPQLMQSSRVVKKAFDLLKERMNSKGREINKAGRILLATVEGDIHDIGKNIVKVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCHHHHHHHHHHHH
FNNYGFEVVDLGVNVKSDDIVEAAFDHKVDIVGLSALMTVTMEKMRETVNILKQKGFKGG
HHCCCCEEEEECCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IIIGGAVTSEDFAREIGADLYAGDALDGVRKVKKYLNLN
EEEECCCCCHHHHHHHCCCHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]