| Definition | Halothermothrix orenii H 168 chromosome, complete genome. |
|---|---|
| Accession | NC_011899 |
| Length | 2,578,146 |
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The map label for this gene is pulA [H]
Identifier: 220932789
GI number: 220932789
Start: 2097006
End: 2099630
Strand: Reverse
Name: pulA [H]
Synonym: Hore_19550
Alternate gene names: 220932789
Gene position: 2099630-2097006 (Counterclockwise)
Preceding gene: 220932790
Following gene: 220932788
Centisome position: 81.44
GC content: 43.73
Gene sequence:
>2625_bases ATGGAAGGAAAATTTGAGAAAAATACTGTTACCCTTGAAGATATAAGACCCAGGGTAAAAAAAGGAGCCACTGAAATACC TGAAGGCCATGTCAGGCTTCATTACCGCCGTTTTGATGAAAATTATGAGGGCTGGGGACTCCATCTCTGGGGTGAAGGTT ATGCCGGACCTCCAGTTACCTGGGGAAGCCCTTTACCTATAACCGGTATTGATGACTATGGTGCTTACTGGGATATTCCG TATAATGAAGGTGTTGGAGACCTCAACTTCATTATACATAAAGGAAATATAAAGGATCCGCAGCCGGACAGGACATATCC TGATCCCGATAAGAATAAAGAAATCTGGTGTGTTTCCGGTGAAGTTAAAGAGTTCCTGAATAAAGAAGAAGCCCTCAATG CTGCCGGTAATAAAGTGGTCAGTGCTGTTATTAAGGACAGGAGAACCATTGAGGTCGAGTTCCGCTCAGAGATCAGTAAA CCGATTTATATCAGGGACGGTTATGATTATGTTCCCCTTAAAAAGCTTGATATGTCCCAGGCTCCCGTTTATGTCCTGAC TACCAGGGAAGACCTTGATTATAATAAAACCTATAAAATTGAGTGCGGGGCCCTGGAAGGCAAGACTATTCTTGGACCTG AAGCCATTGATAAGGCTTTTGCCTATGACGGTGAACTGGGGGCCCTTTATAAACCGGAGGCTACCACCTTTAAACTCTGG GCACCCCTGGCAACTGAGGTTAAATTAAAATTGTTTAAAGAGGGTAATGATCAAAAGCCATATAAAATTGTGCCCATGAA CAAAGGAGATAAAGGTGTCTGGAGTGTAACGGTTGCAGGCGATTTACTTGGTGAATTCTACCAGTATAGTGTTACCAACA ACGGTCAGACCCGGAATGCCCTGGATCCCTATGCTAGATCTATGGCCGGGTTTAACAGTAATGGTCCTGATAAGGTTGGT AAGGGAGCCATTGTTGACCTTGATCTGACTGATCCTGAAGGCTGGAATCAGGATGACTATGTCTCCATTGAAGACCAGGA AGATGTAGTTATCTATGAAATGTCAGTCCGTGATTTTACTATTTCCGTTGATTCAGGAGTTGATTTTGAGAAAAAAGGTA CCTATTTAGGGTTTATTGATAAGATACCTCACTTAAAGGACCTGGGTATTACCCATGTTCAGTTAATGCCGGTTTTAAAC TTCTATTACGGTAATGAGTTCAATAAGGATTTTGAAAATATCGGTAGTGCCGGTGAGGCCAATTATAACTGGGGTTATGA CCCTCATAACTACTTTACACCTGAAGGCTGGTATTCACTGGATCCCTCAGATCCACATCTGAGAATTAAGGAATTAAAAA CCCTCATTAAGGCTTTACACGATGCCGGTATTGGGGTTGTCCTTGATGTAGTTTATAACCATACCGCTAAAACCAGCACC TTTGAAGATATTGTCCCCAACTATTATTACAGGCGTAACCCCGATGGTTCCTTCACCAGTGGTTCCGGTTGTGGTAATGA TACCGCTTCTGAACGGGCCATGATGAGGAAACTTATTGTGGATTCAACCAGATACTGGGTTGAAGAATATCATGTCGATG GTTTCCGGTTTGACCTGATGGGATTACATGATGAGACAACCATGAAAAAGGTTGCCAGCACCTTAAGGTCCATCAATCCT GATGTGGTCCTCCATGGTGAGGGCTGGAATATGGGGACCCTCCCTGTTAAAGACCGTTACATTAAGGGGGCCGGGGCTGA AAGAAGCTGCCTCCAGATGGAACATGCTATTGCCGTCTTTAATGATGGTTTAAGAGATGCCCTGAAACATGAGTATTATG CCTCTCCGCTCCCCGAAGGTGGGTTTGTTCAGAGGCAGAAGAACAAAGAACCCCTTATCAGATCAGGAATAATTGCCGGT ATGGTTGATTACACTTCACCGGTATTGATTGATGGTAATCCCTATAATCGTTTTGCTGATGACCCTGAAGAAACTATTAC CTATGTTACCTGCCATGATGGTTATACTCTCTGGGACAAAATTGTCGGGTCAACTCCCAATGCTACCGAAGAAGAAAGGA AGCAGATGCACAAACTGGCCCATGCAGTAATCCTGACTTCCCAGGGTAAACCCTTTATTCATGGTGGAGCCGAATTGTTA AGGAGTAAACCCGACCCCGATAATGATAGGTATGGGGTCGACCATAACAGTTATGATTCGGGTGATTTGACCAACCAGAT TGACTGGTCCCGTAAGGTTAAATATGCTGATGTCTATGAATACTTTAAGGGGCTTATTGAACTTAGAAAATCACACGAGG CCTTTAGAATGGAAACCATGGAAGAAATCCAGAAGGGGTTAACCTTTATTGAAGAAGATATTCCCTTCCTGGTTGCCTAT CGTCTTGAAGAACAGGATGGAACTGATCCCTGGAAAGAGATAGTGGTTATTTACAATGCTAATAAAGAAAATAAAACGGT TGAGGTTGACGGTGTTGACCCCAGCTGGAAGGTAGTAGTCGATGGTGACAGGGCCGGAGTAGAACCCCTTGCTGAAACCG AAGTAGTTGTCAGGGATGGCGAAGTAGTTGTACCCCCGATTTCCTGTGTGGTTATTCATAAATAA
Upstream 100 bases:
>100_bases ACCTGACAGGGCCTATCCTGATCCCGATAATAACAAAGAAATCTGGTGTGTTACCGGTGATGTTACTGAATACACCAGTA AAGAAGAAGCCATTGCTTCA
Downstream 100 bases:
>100_bases GGGTTCAGGTAAATTAATTCTGGGTAAATATATAGATATATTATTTAGCTAAAAATAAGCTAAAAAACCCCCGGTTTTCA TCCGGGGGTTTTTTTCATAT
Product: pullulanase, type I
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 874; Mature: 874
Protein sequence:
>874_residues MEGKFEKNTVTLEDIRPRVKKGATEIPEGHVRLHYRRFDENYEGWGLHLWGEGYAGPPVTWGSPLPITGIDDYGAYWDIP YNEGVGDLNFIIHKGNIKDPQPDRTYPDPDKNKEIWCVSGEVKEFLNKEEALNAAGNKVVSAVIKDRRTIEVEFRSEISK PIYIRDGYDYVPLKKLDMSQAPVYVLTTREDLDYNKTYKIECGALEGKTILGPEAIDKAFAYDGELGALYKPEATTFKLW APLATEVKLKLFKEGNDQKPYKIVPMNKGDKGVWSVTVAGDLLGEFYQYSVTNNGQTRNALDPYARSMAGFNSNGPDKVG KGAIVDLDLTDPEGWNQDDYVSIEDQEDVVIYEMSVRDFTISVDSGVDFEKKGTYLGFIDKIPHLKDLGITHVQLMPVLN FYYGNEFNKDFENIGSAGEANYNWGYDPHNYFTPEGWYSLDPSDPHLRIKELKTLIKALHDAGIGVVLDVVYNHTAKTST FEDIVPNYYYRRNPDGSFTSGSGCGNDTASERAMMRKLIVDSTRYWVEEYHVDGFRFDLMGLHDETTMKKVASTLRSINP DVVLHGEGWNMGTLPVKDRYIKGAGAERSCLQMEHAIAVFNDGLRDALKHEYYASPLPEGGFVQRQKNKEPLIRSGIIAG MVDYTSPVLIDGNPYNRFADDPEETITYVTCHDGYTLWDKIVGSTPNATEEERKQMHKLAHAVILTSQGKPFIHGGAELL RSKPDPDNDRYGVDHNSYDSGDLTNQIDWSRKVKYADVYEYFKGLIELRKSHEAFRMETMEEIQKGLTFIEEDIPFLVAY RLEEQDGTDPWKEIVVIYNANKENKTVEVDGVDPSWKVVVDGDRAGVEPLAETEVVVRDGEVVVPPISCVVIHK
Sequences:
>Translated_874_residues MEGKFEKNTVTLEDIRPRVKKGATEIPEGHVRLHYRRFDENYEGWGLHLWGEGYAGPPVTWGSPLPITGIDDYGAYWDIP YNEGVGDLNFIIHKGNIKDPQPDRTYPDPDKNKEIWCVSGEVKEFLNKEEALNAAGNKVVSAVIKDRRTIEVEFRSEISK PIYIRDGYDYVPLKKLDMSQAPVYVLTTREDLDYNKTYKIECGALEGKTILGPEAIDKAFAYDGELGALYKPEATTFKLW APLATEVKLKLFKEGNDQKPYKIVPMNKGDKGVWSVTVAGDLLGEFYQYSVTNNGQTRNALDPYARSMAGFNSNGPDKVG KGAIVDLDLTDPEGWNQDDYVSIEDQEDVVIYEMSVRDFTISVDSGVDFEKKGTYLGFIDKIPHLKDLGITHVQLMPVLN FYYGNEFNKDFENIGSAGEANYNWGYDPHNYFTPEGWYSLDPSDPHLRIKELKTLIKALHDAGIGVVLDVVYNHTAKTST FEDIVPNYYYRRNPDGSFTSGSGCGNDTASERAMMRKLIVDSTRYWVEEYHVDGFRFDLMGLHDETTMKKVASTLRSINP DVVLHGEGWNMGTLPVKDRYIKGAGAERSCLQMEHAIAVFNDGLRDALKHEYYASPLPEGGFVQRQKNKEPLIRSGIIAG MVDYTSPVLIDGNPYNRFADDPEETITYVTCHDGYTLWDKIVGSTPNATEEERKQMHKLAHAVILTSQGKPFIHGGAELL RSKPDPDNDRYGVDHNSYDSGDLTNQIDWSRKVKYADVYEYFKGLIELRKSHEAFRMETMEEIQKGLTFIEEDIPFLVAY RLEEQDGTDPWKEIVVIYNANKENKTVEVDGVDPSWKVVVDGDRAGVEPLAETEVVVRDGEVVVPPISCVVIHK >Mature_874_residues MEGKFEKNTVTLEDIRPRVKKGATEIPEGHVRLHYRRFDENYEGWGLHLWGEGYAGPPVTWGSPLPITGIDDYGAYWDIP YNEGVGDLNFIIHKGNIKDPQPDRTYPDPDKNKEIWCVSGEVKEFLNKEEALNAAGNKVVSAVIKDRRTIEVEFRSEISK PIYIRDGYDYVPLKKLDMSQAPVYVLTTREDLDYNKTYKIECGALEGKTILGPEAIDKAFAYDGELGALYKPEATTFKLW APLATEVKLKLFKEGNDQKPYKIVPMNKGDKGVWSVTVAGDLLGEFYQYSVTNNGQTRNALDPYARSMAGFNSNGPDKVG KGAIVDLDLTDPEGWNQDDYVSIEDQEDVVIYEMSVRDFTISVDSGVDFEKKGTYLGFIDKIPHLKDLGITHVQLMPVLN FYYGNEFNKDFENIGSAGEANYNWGYDPHNYFTPEGWYSLDPSDPHLRIKELKTLIKALHDAGIGVVLDVVYNHTAKTST FEDIVPNYYYRRNPDGSFTSGSGCGNDTASERAMMRKLIVDSTRYWVEEYHVDGFRFDLMGLHDETTMKKVASTLRSINP DVVLHGEGWNMGTLPVKDRYIKGAGAERSCLQMEHAIAVFNDGLRDALKHEYYASPLPEGGFVQRQKNKEPLIRSGIIAG MVDYTSPVLIDGNPYNRFADDPEETITYVTCHDGYTLWDKIVGSTPNATEEERKQMHKLAHAVILTSQGKPFIHGGAELL RSKPDPDNDRYGVDHNSYDSGDLTNQIDWSRKVKYADVYEYFKGLIELRKSHEAFRMETMEEIQKGLTFIEEDIPFLVAY RLEEQDGTDPWKEIVVIYNANKENKTVEVDGVDPSWKVVVDGDRAGVEPLAETEVVVRDGEVVVPPISCVVIHK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=609, Percent_Identity=26.7651888341544, Blast_Score=138, Evalue=1e-33,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 98799; Mature: 98799
Theoretical pI: Translated: 4.72; Mature: 4.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEGKFEKNTVTLEDIRPRVKKGATEIPEGHVRLHYRRFDENYEGWGLHLWGEGYAGPPVT CCCCCCCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCC WGSPLPITGIDDYGAYWDIPYNEGVGDLNFIIHKGNIKDPQPDRTYPDPDKNKEIWCVSG CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECH EVKEFLNKEEALNAAGNKVVSAVIKDRRTIEVEFRSEISKPIYIRDGYDYVPLKKLDMSQ HHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCEEEECCCCCCCCCCCCCCC APVYVLTTREDLDYNKTYKIECGALEGKTILGPEAIDKAFAYDGELGALYKPEATTFKLW CCEEEEEECCCCCCCCEEEEEECCCCCCEEECHHHHHHHHCCCCCCCCEECCCCCEEEEE APLATEVKLKLFKEGNDQKPYKIVPMNKGDKGVWSVTVAGDLLGEFYQYSVTNNGQTRNA CCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHEEEECCCCCCCCC LDPYARSMAGFNSNGPDKVGKGAIVDLDLTDPEGWNQDDYVSIEDQEDVVIYEMSVRDFT CCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEEEEEEEEEEE ISVDSGVDFEKKGTYLGFIDKIPHLKDLGITHVQLMPVLNFYYGNEFNKDFENIGSAGEA EEECCCCCHHHCCCEEEHHHCCCCHHHCCCCEEEHHHHHHHHHCCCCCCCHHHCCCCCCC NYNWGYDPHNYFTPEGWYSLDPSDPHLRIKELKTLIKALHDAGIGVVLDVVYNHTAKTST CCCCCCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCC FEDIVPNYYYRRNPDGSFTSGSGCGNDTASERAMMRKLIVDSTRYWVEEYHVDGFRFDLM HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE GLHDETTMKKVASTLRSINPDVVLHGEGWNMGTLPVKDRYIKGAGAERSCLQMEHAIAVF ECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH NDGLRDALKHEYYASPLPEGGFVQRQKNKEPLIRSGIIAGMVDYTSPVLIDGNPYNRFAD HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCC DPEETITYVTCHDGYTLWDKIVGSTPNATEEERKQMHKLAHAVILTSQGKPFIHGGAELL CCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEECCHHHH RSKPDPDNDRYGVDHNSYDSGDLTNQIDWSRKVKYADVYEYFKGLIELRKSHEAFRMETM CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH EEIQKGLTFIEEDIPFLVAYRLEEQDGTDPWKEIVVIYNANKENKTVEVDGVDPSWKVVV HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHEEEEEEECCCCCCEEEEECCCCCCEEEE DGDRAGVEPLAETEVVVRDGEVVVPPISCVVIHK CCCCCCCCCCCCCEEEEECCCEEECCCEEEEEEC >Mature Secondary Structure MEGKFEKNTVTLEDIRPRVKKGATEIPEGHVRLHYRRFDENYEGWGLHLWGEGYAGPPVT CCCCCCCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCC WGSPLPITGIDDYGAYWDIPYNEGVGDLNFIIHKGNIKDPQPDRTYPDPDKNKEIWCVSG CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECH EVKEFLNKEEALNAAGNKVVSAVIKDRRTIEVEFRSEISKPIYIRDGYDYVPLKKLDMSQ HHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCEEEECCCCCCCCCCCCCCC APVYVLTTREDLDYNKTYKIECGALEGKTILGPEAIDKAFAYDGELGALYKPEATTFKLW CCEEEEEECCCCCCCCEEEEEECCCCCCEEECHHHHHHHHCCCCCCCCEECCCCCEEEEE APLATEVKLKLFKEGNDQKPYKIVPMNKGDKGVWSVTVAGDLLGEFYQYSVTNNGQTRNA CCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHHHEEEECCCCCCCCC LDPYARSMAGFNSNGPDKVGKGAIVDLDLTDPEGWNQDDYVSIEDQEDVVIYEMSVRDFT CCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEEEEEEEEEEEE ISVDSGVDFEKKGTYLGFIDKIPHLKDLGITHVQLMPVLNFYYGNEFNKDFENIGSAGEA EEECCCCCHHHCCCEEEHHHCCCCHHHCCCCEEEHHHHHHHHHCCCCCCCHHHCCCCCCC NYNWGYDPHNYFTPEGWYSLDPSDPHLRIKELKTLIKALHDAGIGVVLDVVYNHTAKTST CCCCCCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCC FEDIVPNYYYRRNPDGSFTSGSGCGNDTASERAMMRKLIVDSTRYWVEEYHVDGFRFDLM HHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE GLHDETTMKKVASTLRSINPDVVLHGEGWNMGTLPVKDRYIKGAGAERSCLQMEHAIAVF ECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH NDGLRDALKHEYYASPLPEGGFVQRQKNKEPLIRSGIIAGMVDYTSPVLIDGNPYNRFAD HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCC DPEETITYVTCHDGYTLWDKIVGSTPNATEEERKQMHKLAHAVILTSQGKPFIHGGAELL CCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEECCHHHH RSKPDPDNDRYGVDHNSYDSGDLTNQIDWSRKVKYADVYEYFKGLIELRKSHEAFRMETM CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH EEIQKGLTFIEEDIPFLVAYRLEEQDGTDPWKEIVVIYNANKENKTVEVDGVDPSWKVVV HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHEEEEEEECCCCCCEEEEECCCCCCEEEE DGDRAGVEPLAETEVVVRDGEVVVPPISCVVIHK CCCCCCCCCCCCCEEEEECCCEEECCCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]