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The map label for this gene is mtbC1 [H]

Identifier: 220932372

GI number: 220932372

Start: 1643890

End: 1644522

Strand: Reverse

Name: mtbC1 [H]

Synonym: Hore_15360

Alternate gene names: 220932372

Gene position: 1644522-1643890 (Counterclockwise)

Preceding gene: 220932373

Following gene: 220932371

Centisome position: 63.79

GC content: 42.34

Gene sequence:

>633_bases
ATGGGAATATTGAATGAAATATCAGAACATTTACAGGCCGGAGATGTAAACGGGGTAACAGATGGTATTAAAAAGGCCCT
GGATGATGGGGTGACCCCTAAAGAAATCTTGAATACTGGTTTAATTGGCGGAATGAGTATTATCGGTGAAAAATTCAAAA
ACAATGAAGTATTTGTTCCTGAAGTATTGATAGCAGCCCGGGCCATGAATGCCGGTATGGATATTTTGAAACCAAAATTG
ATTGAAACCGGGGTTGATTACATCGGTAAGTTTGTTATTGGAACGGTTAAAGGGGACCTCCATGATATTGGTAAAAACCT
GGTCAAGATGATGTTTGAGGGTAACGGTTTTGAGGTAATTGACCTGGGAACAGATGTGGCTCCAGAACAATTCGTGGCAG
CTGTTAAGGAGCATAAACCCGATATTGTAGGTATGTCTGCTTTATTAACAACAACAATGCCTGAGATGGAAAACACTATT
AAAGCCCTCAAAGATGAGGGGCTCCGGGATGATGTAGCGGTTATGATTGGAGGGGCTCCTGTTACTGATAAGTTTGCCAG
TGAAATCGGGGCTGATGCCTATGCTAAAGATGCGGCTACCGCGGCTACAATGGCCAAAAAATTCCTGTCTTAA

Upstream 100 bases:

>100_bases
AAAAATCTTACAATACAAATCATAAATATAATTATACAATAATAACAAAATATATAGTTATAATATAAAAATATGAGGGA
CAATAGAGGAGGATATACTG

Downstream 100 bases:

>100_bases
TACCCTTTAATGAAGACTTTTTTAAAAGGCATTTGAGGAGTCGGGCTTATCGGTCTGGCTCCTTTTTTAATATATTCCTT
TAAATTACGGCAGAAGGTTA

Product: Methionine synthase

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MGILNEISEHLQAGDVNGVTDGIKKALDDGVTPKEILNTGLIGGMSIIGEKFKNNEVFVPEVLIAARAMNAGMDILKPKL
IETGVDYIGKFVIGTVKGDLHDIGKNLVKMMFEGNGFEVIDLGTDVAPEQFVAAVKEHKPDIVGMSALLTTTMPEMENTI
KALKDEGLRDDVAVMIGGAPVTDKFASEIGADAYAKDAATAATMAKKFLS

Sequences:

>Translated_210_residues
MGILNEISEHLQAGDVNGVTDGIKKALDDGVTPKEILNTGLIGGMSIIGEKFKNNEVFVPEVLIAARAMNAGMDILKPKL
IETGVDYIGKFVIGTVKGDLHDIGKNLVKMMFEGNGFEVIDLGTDVAPEQFVAAVKEHKPDIVGMSALLTTTMPEMENTI
KALKDEGLRDDVAVMIGGAPVTDKFASEIGADAYAKDAATAATMAKKFLS
>Mature_209_residues
GILNEISEHLQAGDVNGVTDGIKKALDDGVTPKEILNTGLIGGMSIIGEKFKNNEVFVPEVLIAARAMNAGMDILKPKLI
ETGVDYIGKFVIGTVKGDLHDIGKNLVKMMFEGNGFEVIDLGTDVAPEQFVAAVKEHKPDIVGMSALLTTTMPEMENTIK
ALKDEGLRDDVAVMIGGAPVTDKFASEIGADAYAKDAATAATMAKKFLS

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=160, Percent_Identity=35, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1790450, Length=184, Percent_Identity=34.2391304347826, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535405, Length=160, Percent_Identity=35.625, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 22238; Mature: 22107

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILNEISEHLQAGDVNGVTDGIKKALDDGVTPKEILNTGLIGGMSIIGEKFKNNEVFVP
CCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEHH
EVLIAARAMNAGMDILKPKLIETGVDYIGKFVIGTVKGDLHDIGKNLVKMMFEGNGFEVI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DLGTDVAPEQFVAAVKEHKPDIVGMSALLTTTMPEMENTIKALKDEGLRDDVAVMIGGAP
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
VTDKFASEIGADAYAKDAATAATMAKKFLS
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GILNEISEHLQAGDVNGVTDGIKKALDDGVTPKEILNTGLIGGMSIIGEKFKNNEVFVP
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEHH
EVLIAARAMNAGMDILKPKLIETGVDYIGKFVIGTVKGDLHDIGKNLVKMMFEGNGFEVI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
DLGTDVAPEQFVAAVKEHKPDIVGMSALLTTTMPEMENTIKALKDEGLRDDVAVMIGGAP
ECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
VTDKFASEIGADAYAKDAATAATMAKKFLS
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]