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The map label for this gene is tig

Identifier: 220932331

GI number: 220932331

Start: 1593560

End: 1594849

Strand: Reverse

Name: tig

Synonym: Hore_14950

Alternate gene names: 220932331

Gene position: 1594849-1593560 (Counterclockwise)

Preceding gene: 220932332

Following gene: 220932330

Centisome position: 61.86

GC content: 41.78

Gene sequence:

>1290_bases
ATGGAAGTAAAAAAAGAACAATTAGAGGGAAATAAGGTGGAATTAAAAGTTGAAATAGAGCCGGAGAGAGTTGATAAGGC
TCTGGAACAGGCATACCGGAAGGTAGTTAAAAATGTTTCTATCCCGGGTTTCCGTAAAGGAAAAGTTCCTCGCAGGGTTT
TAGAAGCAAGATATGGCAAGGAAGTTCTCCATCGTGATGCGTTTGATATCCTTGTCCCCCCTGCATACCAGGAGGCAGTT
CAGGCAGCAGAGATTGAACCTATTGATAGACCAGAGATTACTGATTTTTATATTGAGGAAAATAAACCGGCTACCTTCAG
TGCTGTAGTTGAGGTTAAACCTGAGGTTGAGCTCGGTGAATACAAAGGTCTCGGAATAGAAAAAGATGAAGTAGAAGTTA
CCGAAGAGGATATTGAGGCTCAGCTGAAGAGCCTTCAGGAACAACATAGCCAGCTTAAAAGTACCGACAAAGAAGTTGTA
GAAGAGGGAGATTTTGTTGTTATTGACTTTGTGGGAACTATAGATGGTGAAGAGTTCCAGGGTGGTAGTGCCGAAGAGTA
TAACCTGGAGGTAGGTTCCGGTACTTTTATTCCCGGTTTTGAAGAACAGCTTGTCGGGAAAAAGGTAGGAGAGGAAACCG
AGGTTGAGGTTACCTTCCCTGAGGATTATCAGGCTGAGGATTTAGCCGGGAAGGATGCCGTCTTTAAAGTTGATATAAAG
GAGATCAAGGTTAAAGAGACTCCCGAACTGGATGATGAATTTGCTAAAGAGGCCAGTGAGTTTGATACTCTTGAAGAATT
AAAAGATGATATTAAAGAGAGACTAACCAGCCAAAAAGAAGACAGGGCCCGGCGAAAACTGGAAGATGAGATTGTTGACA
GGGTCACTGAAAATGCTGAAGTAGATGTTCCTGAAACCCTGGTTAATAATGAACTGGATATGATGTACCAGAATTTAGCC
TATAGTATTTCTTCATATGGCATGAAGGTAGAAGACTACCTGAAGTCCATGGGTCTTGATGAAGAATCATGGCGTGAAGA
AAATAGAGAAGAGGCTGAAAAGCGGGCTAAATCTAATCTGGTCCTGGAAGCAATCGGGAAAAAAGAAGGAATTGAAGTTA
CTGATGAAGAAATAGACAATAAGATCGAGGAAATAGCTAAAGATGGTGAACAGAAACCTGAACAGATAAAAGCCTACCTG
CAGCTTCAGGGACAGCTTGAAGGTTTGAAGCATACTATATTCGTTAGAAAAGTCATAGATTTCCTGGTGGAACACAACCA
GAATGACTAG

Upstream 100 bases:

>100_bases
GTATGTAAATAATGTAAAATGCTAAAATGTTAAAAAGTATTTCATTTAACAGGGAAAATACATAAATAACATTATTATAT
TAGTAAGGGGGATTATAAAC

Downstream 100 bases:

>100_bases
AATAGAGTAGGTTTAAATTATTTATAATCGGGAATAATGAAATTAAGAGGAGGGATTTTACTATGAGTTTGATACCTGTT
GTTGTTGAACAGACTAACAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MEVKKEQLEGNKVELKVEIEPERVDKALEQAYRKVVKNVSIPGFRKGKVPRRVLEARYGKEVLHRDAFDILVPPAYQEAV
QAAEIEPIDRPEITDFYIEENKPATFSAVVEVKPEVELGEYKGLGIEKDEVEVTEEDIEAQLKSLQEQHSQLKSTDKEVV
EEGDFVVIDFVGTIDGEEFQGGSAEEYNLEVGSGTFIPGFEEQLVGKKVGEETEVEVTFPEDYQAEDLAGKDAVFKVDIK
EIKVKETPELDDEFAKEASEFDTLEELKDDIKERLTSQKEDRARRKLEDEIVDRVTENAEVDVPETLVNNELDMMYQNLA
YSISSYGMKVEDYLKSMGLDEESWREENREEAEKRAKSNLVLEAIGKKEGIEVTDEEIDNKIEEIAKDGEQKPEQIKAYL
QLQGQLEGLKHTIFVRKVIDFLVEHNQND

Sequences:

>Translated_429_residues
MEVKKEQLEGNKVELKVEIEPERVDKALEQAYRKVVKNVSIPGFRKGKVPRRVLEARYGKEVLHRDAFDILVPPAYQEAV
QAAEIEPIDRPEITDFYIEENKPATFSAVVEVKPEVELGEYKGLGIEKDEVEVTEEDIEAQLKSLQEQHSQLKSTDKEVV
EEGDFVVIDFVGTIDGEEFQGGSAEEYNLEVGSGTFIPGFEEQLVGKKVGEETEVEVTFPEDYQAEDLAGKDAVFKVDIK
EIKVKETPELDDEFAKEASEFDTLEELKDDIKERLTSQKEDRARRKLEDEIVDRVTENAEVDVPETLVNNELDMMYQNLA
YSISSYGMKVEDYLKSMGLDEESWREENREEAEKRAKSNLVLEAIGKKEGIEVTDEEIDNKIEEIAKDGEQKPEQIKAYL
QLQGQLEGLKHTIFVRKVIDFLVEHNQND
>Mature_429_residues
MEVKKEQLEGNKVELKVEIEPERVDKALEQAYRKVVKNVSIPGFRKGKVPRRVLEARYGKEVLHRDAFDILVPPAYQEAV
QAAEIEPIDRPEITDFYIEENKPATFSAVVEVKPEVELGEYKGLGIEKDEVEVTEEDIEAQLKSLQEQHSQLKSTDKEVV
EEGDFVVIDFVGTIDGEEFQGGSAEEYNLEVGSGTFIPGFEEQLVGKKVGEETEVEVTFPEDYQAEDLAGKDAVFKVDIK
EIKVKETPELDDEFAKEASEFDTLEELKDDIKERLTSQKEDRARRKLEDEIVDRVTENAEVDVPETLVNNELDMMYQNLA
YSISSYGMKVEDYLKSMGLDEESWREENREEAEKRAKSNLVLEAIGKKEGIEVTDEEIDNKIEEIAKDGEQKPEQIKAYL
QLQGQLEGLKHTIFVRKVIDFLVEHNQND

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=400, Percent_Identity=32.25, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_HALOH (B8CY75)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002509239.1
- ProteinModelPortal:   B8CY75
- GeneID:   7313086
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_14950
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   CLSK2806468
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49043; Mature: 49043

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKKEQLEGNKVELKVEIEPERVDKALEQAYRKVVKNVSIPGFRKGKVPRRVLEARYGK
CCCCHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EVLHRDAFDILVPPAYQEAVQAAEIEPIDRPEITDFYIEENKPATFSAVVEVKPEVELGE
HHHHCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCC
YKGLGIEKDEVEVTEEDIEAQLKSLQEQHSQLKSTDKEVVEEGDFVVIDFVGTIDGEEFQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GGSAEEYNLEVGSGTFIPGFEEQLVGKKVGEETEVEVTFPEDYQAEDLAGKDAVFKVDIK
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCCCCEEEEEHH
EIKVKETPELDDEFAKEASEFDTLEELKDDIKERLTSQKEDRARRKLEDEIVDRVTENAE
HEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
VDVPETLVNNELDMMYQNLAYSISSYGMKVEDYLKSMGLDEESWREENREEAEKRAKSNL
CCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VLEAIGKKEGIEVTDEEIDNKIEEIAKDGEQKPEQIKAYLQLQGQLEGLKHTIFVRKVID
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVEHNQND
HHHHCCCCC
>Mature Secondary Structure
MEVKKEQLEGNKVELKVEIEPERVDKALEQAYRKVVKNVSIPGFRKGKVPRRVLEARYGK
CCCCHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EVLHRDAFDILVPPAYQEAVQAAEIEPIDRPEITDFYIEENKPATFSAVVEVKPEVELGE
HHHHCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCC
YKGLGIEKDEVEVTEEDIEAQLKSLQEQHSQLKSTDKEVVEEGDFVVIDFVGTIDGEEFQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GGSAEEYNLEVGSGTFIPGFEEQLVGKKVGEETEVEVTFPEDYQAEDLAGKDAVFKVDIK
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCCCCEEEEEHH
EIKVKETPELDDEFAKEASEFDTLEELKDDIKERLTSQKEDRARRKLEDEIVDRVTENAE
HEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
VDVPETLVNNELDMMYQNLAYSISSYGMKVEDYLKSMGLDEESWREENREEAEKRAKSNL
CCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VLEAIGKKEGIEVTDEEIDNKIEEIAKDGEQKPEQIKAYLQLQGQLEGLKHTIFVRKVID
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVEHNQND
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA