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The map label for this gene is rnfB [H]

Identifier: 220932266

GI number: 220932266

Start: 1516638

End: 1517633

Strand: Reverse

Name: rnfB [H]

Synonym: Hore_14300

Alternate gene names: 220932266

Gene position: 1517633-1516638 (Counterclockwise)

Preceding gene: 220932267

Following gene: 220932265

Centisome position: 58.87

GC content: 46.79

Gene sequence:

>996_bases
ATGGAACCAGTATATGTATATTCTCTCCTCAGCATGGGAGGCCTCGGGGCCTTACTGGCAGCTGGTCTGGGTATAGCTTC
AAGAACTTTCCATGTTGAGAGGGATAAAAGAATAGATGAAGTTGAAGAAGCCTTACCAGGGGCCAATTGTGGGGCCTGTG
GTTATGCTGGATGCAGTAGTTTTGCGGAAGCGGTAGTTAAAGGAGAAGCCCCTGTTAACGGTTGTCCGGTCGGAGGGGCT
GAGGTGGCTGATAAGATTGCTGATATAATGGGACTTGAAGCAGAAGCTGGTGAGAAAAAGGTTGCCCAGGTTTTATGTAA
TGGTGGCTGGAAGGAAACCCGTCAGCCTGCTGAATATATGGGTATCGAAAGCTGTAAGGCTGCCAATATGGTTAATGGGG
GAACAAAGGCCTGTCAGTATGGCTGCCTTGGCCTGGGTGACTGTGTTGCTGTCTGCCCTTTTGATGCTATTGAAATGAAT
GAAAATGGTCTTCCGGAAGTAAATTATGATAAATGCACTGGCTGTGGCAAATGTGTAGAGGCCTGTCCCCGTGGTATTAT
TACCCTGGCCCCCCTTTCAGGGAAAAATCATATCAGGTGTTCTTCCCATGACCATGGGAAAGTAGTAAAAGGTGTCTGTG
AAGTGGGTTGTATAGGCTGTGGGATCTGTGCCCGGGTTTGTCCGGTCGATGCCATCACCATTGAAGATAACCTTGCCGTT
ATCGATTATGATAAGTGTATTAACTGTGGCCTCTGTGCTGAGAAGTGTCCAACCGGTGCCATTGAGTTTGAGGGCCGGAG
GATTGAGGAAATTCATATTACTGATAAATGTGTTGGTTGTACCAGGTGTGCCAGAGCCTGTCCGGTTGATGCTATTGAAG
GGTCTTTAAAGGAAAAACATGAAATTAACCCTGAAACCTGTGTTAAGTGCGGTATATGTTATGATACCTGTAAGGTAAAA
GGAGCCATTGAGGTTACCTATCAGGTGGAAGAGTAA

Upstream 100 bases:

>100_bases
AAAAGGGGTTCCCATAACCCTGATTATTGCCGGGATTATGGCTATGGCTTTTATGGGTTTTTCCGGTATGATTTCCATTT
AAGTTTACGGAGGTGAAAGG

Downstream 100 bases:

>100_bases
TTTATAAAATGAGGAAGAGTAATTTATAAAATAAGAATAATTTATAAAAAATTAGTTAACTTAAAATGTTAAAAAAACAG
GGGTACTGTGGTGCCCCTGT

Product: RnfABCDGE type electron transport complex subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MEPVYVYSLLSMGGLGALLAAGLGIASRTFHVERDKRIDEVEEALPGANCGACGYAGCSSFAEAVVKGEAPVNGCPVGGA
EVADKIADIMGLEAEAGEKKVAQVLCNGGWKETRQPAEYMGIESCKAANMVNGGTKACQYGCLGLGDCVAVCPFDAIEMN
ENGLPEVNYDKCTGCGKCVEACPRGIITLAPLSGKNHIRCSSHDHGKVVKGVCEVGCIGCGICARVCPVDAITIEDNLAV
IDYDKCINCGLCAEKCPTGAIEFEGRRIEEIHITDKCVGCTRCARACPVDAIEGSLKEKHEINPETCVKCGICYDTCKVK
GAIEVTYQVEE

Sequences:

>Translated_331_residues
MEPVYVYSLLSMGGLGALLAAGLGIASRTFHVERDKRIDEVEEALPGANCGACGYAGCSSFAEAVVKGEAPVNGCPVGGA
EVADKIADIMGLEAEAGEKKVAQVLCNGGWKETRQPAEYMGIESCKAANMVNGGTKACQYGCLGLGDCVAVCPFDAIEMN
ENGLPEVNYDKCTGCGKCVEACPRGIITLAPLSGKNHIRCSSHDHGKVVKGVCEVGCIGCGICARVCPVDAITIEDNLAV
IDYDKCINCGLCAEKCPTGAIEFEGRRIEEIHITDKCVGCTRCARACPVDAIEGSLKEKHEINPETCVKCGICYDTCKVK
GAIEVTYQVEE
>Mature_331_residues
MEPVYVYSLLSMGGLGALLAAGLGIASRTFHVERDKRIDEVEEALPGANCGACGYAGCSSFAEAVVKGEAPVNGCPVGGA
EVADKIADIMGLEAEAGEKKVAQVLCNGGWKETRQPAEYMGIESCKAANMVNGGTKACQYGCLGLGDCVAVCPFDAIEMN
ENGLPEVNYDKCTGCGKCVEACPRGIITLAPLSGKNHIRCSSHDHGKVVKGVCEVGCIGCGICARVCPVDAITIEDNLAV
IDYDKCINCGLCAEKCPTGAIEFEGRRIEEIHITDKCVGCTRCARACPVDAIEGSLKEKHEINPETCVKCGICYDTCKVK
GAIEVTYQVEE

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=193, Percent_Identity=30.0518134715026, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 34768; Mature: 34768

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
11.5 %Cys+Met (Translated Protein)
9.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
11.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVYVYSLLSMGGLGALLAAGLGIASRTFHVERDKRIDEVEEALPGANCGACGYAGCSS
CCCEEEEHHHHHCCHHHHHHHCCCHHHHEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHH
FAEAVVKGEAPVNGCPVGGAEVADKIADIMGLEAEAGEKKVAQVLCNGGWKETRQPAEYM
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCHHHH
GIESCKAANMVNGGTKACQYGCLGLGDCVAVCPFDAIEMNENGLPEVNYDKCTGCGKCVE
CCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCCCCCEECCCCCCCCCCCHHCCCHHHHHH
ACPRGIITLAPLSGKNHIRCSSHDHGKVVKGVCEVGCIGCGICARVCPVDAITIEDNLAV
HCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
IDYDKCINCGLCAEKCPTGAIEFEGRRIEEIHITDKCVGCTRCARACPVDAIEGSLKEKH
EEHHHHCCCCCCHHHCCCCCEEECCCEEEEEEECHHHHHHHHHHHHCCHHHHHCCHHHHH
EINPETCVKCGICYDTCKVKGAIEVTYQVEE
CCCHHHHHHHCCCHHHHEECEEEEEEEEECC
>Mature Secondary Structure
MEPVYVYSLLSMGGLGALLAAGLGIASRTFHVERDKRIDEVEEALPGANCGACGYAGCSS
CCCEEEEHHHHHCCHHHHHHHCCCHHHHEEEECHHHHHHHHHHHCCCCCCCCCCHHHHHH
FAEAVVKGEAPVNGCPVGGAEVADKIADIMGLEAEAGEKKVAQVLCNGGWKETRQPAEYM
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCHHHH
GIESCKAANMVNGGTKACQYGCLGLGDCVAVCPFDAIEMNENGLPEVNYDKCTGCGKCVE
CCHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCCCCCEECCCCCCCCCCCHHCCCHHHHHH
ACPRGIITLAPLSGKNHIRCSSHDHGKVVKGVCEVGCIGCGICARVCPVDAITIEDNLAV
HCCCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEE
IDYDKCINCGLCAEKCPTGAIEFEGRRIEEIHITDKCVGCTRCARACPVDAIEGSLKEKH
EEHHHHCCCCCCHHHCCCCCEEECCCEEEEEEECHHHHHHHHHHHHCCHHHHHCCHHHHH
EINPETCVKCGICYDTCKVKGAIEVTYQVEE
CCCHHHHHHHCCCHHHHEECEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA