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The map label for this gene is grpE [H]

Identifier: 220932118

GI number: 220932118

Start: 1374944

End: 1375639

Strand: Reverse

Name: grpE [H]

Synonym: Hore_12810

Alternate gene names: 220932118

Gene position: 1375639-1374944 (Counterclockwise)

Preceding gene: 220932119

Following gene: 220932117

Centisome position: 53.36

GC content: 34.2

Gene sequence:

>696_bases
ATGGAAAAAGAAAAGCATGTAAGTTCTAATGAAGAATTTAACGAAAAGGAAGAAAAAAATAACGTTGAAACAGTGAGAAA
TAATGAAAATAATGATCAAGAAGTTGAAAATAATGATGAATCTATTCAGATCGATAACAAATCACAGGATAGGACAAATA
ATAAAGAAGACAATGGGGACAATAAACAGGAACAGGAAGATAAGAACGGCTCAGATGAAGATATAAAACACCTTAAAGAG
CGGGTTAAAGAGCTCGAAACGGAAGTAGACGAATTGACTGAAGAAAAGAATAACATATTTAATAAACTTCAGAGGTTGCA
GGCAGATTTTATTAATTACCGGAAAAGAACTAATAAAGAAAAGGGGAAGATTGGTATCAGGGCTAAAATTGAATTGATTG
AAAAAATTTTGCCCGTTGTTGATAATTTTGAACGGGCTTTAAATTCTGCCCCTGATGAGGATGAATTTAAACAGGGGGTT
GATATGATTTACCGGCAGCTAATGGATACCTTAAAAAAAGAAGGGGTTGAAGTTATACCGGCTGTTGGTGAACCCTTTGA
CCATAACCTTCATGAAGCTATAATGCAGGTTGAAGATTCAAAGTATGAATCCGGTACTGTAGTAGAGGAATTACAAAAAG
GCTACATACTTGAAGATAAAGTAATCAGGCCTGCTATGGTTAAAGTTGCTAAATAA

Upstream 100 bases:

>100_bases
TGGTCCTACAAGGATGCATTATCCCATGGTAGTTTCTACAGTAAATTCACTTTCAAAAATTGTTGGCAAAATAATATCTA
GAATGGATGGGTGATGTGTC

Downstream 100 bases:

>100_bases
TCTATAAGTTAAAAAGGAAAGATAAGGGGGAATTGAAATGGGTAAAATTATAGGTATTGACTTAGGGACAACTAACTCAT
GTGTAGCCGTTCTCGAAGGT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MEKEKHVSSNEEFNEKEEKNNVETVRNNENNDQEVENNDESIQIDNKSQDRTNNKEDNGDNKQEQEDKNGSDEDIKHLKE
RVKELETEVDELTEEKNNIFNKLQRLQADFINYRKRTNKEKGKIGIRAKIELIEKILPVVDNFERALNSAPDEDEFKQGV
DMIYRQLMDTLKKEGVEVIPAVGEPFDHNLHEAIMQVEDSKYESGTVVEELQKGYILEDKVIRPAMVKVAK

Sequences:

>Translated_231_residues
MEKEKHVSSNEEFNEKEEKNNVETVRNNENNDQEVENNDESIQIDNKSQDRTNNKEDNGDNKQEQEDKNGSDEDIKHLKE
RVKELETEVDELTEEKNNIFNKLQRLQADFINYRKRTNKEKGKIGIRAKIELIEKILPVVDNFERALNSAPDEDEFKQGV
DMIYRQLMDTLKKEGVEVIPAVGEPFDHNLHEAIMQVEDSKYESGTVVEELQKGYILEDKVIRPAMVKVAK
>Mature_231_residues
MEKEKHVSSNEEFNEKEEKNNVETVRNNENNDQEVENNDESIQIDNKSQDRTNNKEDNGDNKQEQEDKNGSDEDIKHLKE
RVKELETEVDELTEEKNNIFNKLQRLQADFINYRKRTNKEKGKIGIRAKIELIEKILPVVDNFERALNSAPDEDEFKQGV
DMIYRQLMDTLKKEGVEVIPAVGEPFDHNLHEAIMQVEDSKYESGTVVEELQKGYILEDKVIRPAMVKVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=167, Percent_Identity=35.9281437125749, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=35.1190476190476, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653432, Length=199, Percent_Identity=29.6482412060301, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 26947; Mature: 26947

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEKHVSSNEEFNEKEEKNNVETVRNNENNDQEVENNDESIQIDNKSQDRTNNKEDNGD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCC
NKQEQEDKNGSDEDIKHLKERVKELETEVDELTEEKNNIFNKLQRLQADFINYRKRTNKE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KGKIGIRAKIELIEKILPVVDNFERALNSAPDEDEFKQGVDMIYRQLMDTLKKEGVEVIP
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEC
AVGEPFDHNLHEAIMQVEDSKYESGTVVEELQKGYILEDKVIRPAMVKVAK
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKEKHVSSNEEFNEKEEKNNVETVRNNENNDQEVENNDESIQIDNKSQDRTNNKEDNGD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCC
NKQEQEDKNGSDEDIKHLKERVKELETEVDELTEEKNNIFNKLQRLQADFINYRKRTNKE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KGKIGIRAKIELIEKILPVVDNFERALNSAPDEDEFKQGVDMIYRQLMDTLKKEGVEVIP
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEC
AVGEPFDHNLHEAIMQVEDSKYESGTVVEELQKGYILEDKVIRPAMVKVAK
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA