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The map label for this gene is glyS

Identifier: 220932081

GI number: 220932081

Start: 1338399

End: 1340459

Strand: Reverse

Name: glyS

Synonym: Hore_12440

Alternate gene names: 220932081

Gene position: 1340459-1338399 (Counterclockwise)

Preceding gene: 220932082

Following gene: 220932080

Centisome position: 51.99

GC content: 38.43

Gene sequence:

>2061_bases
ATGGCTAGAGACCTTCTTTTTGAAATAGGGACAGAAGAGATGCCAGCTGGATTAATAGGGAAAATAAGGTCTGATTTAAA
AGACCTGGCTATAAATACCCTGGAGGATAAAAGACTTGACTTTAAAGAATGTAAGGTCTTTTCGACCCCCAGAAGACTGG
TTCTTTTTGTTAAAGAGCTTGCCGAAAAACAGGAGGAAAAAAGGGAAGTAGTCAAGGGCCCTGCCCGGTCTATTGCCTTT
GAAGATGATGGTACTCCTACCAGAGCAGCTAAAGGTTTTTCCAGGGCCCAGGGTGTTGAGGTAGATGACCTCATTATAAA
AGATGATTATGTTTATGTTGAAAAGATAGAACAGGGTCAGGATACAGCAGGGTTATTAAAGGATATTCTGCCCGGGCTTA
TTAATAAATTACCACTACCCCGGTCTATGCGGTGGGCTGATTATAATTTTAAATTTATCAGACCTATCAGGTGGCTGTTA
GCTCTGTTCGGTGAAGAAATAATTAGTTTTTCAATGGCCGGTGTTCAAAGTGGTGCCTATACCAGGGGACATAGATTTCT
GGTAAAAGACAGGCTGGAAGTGAAAGATCCTGACCATTATTTTGATGTTATGGAAAAGGGTTATATAATTGTCGATCATA
ATAAAAGGCGGGAACTTATTTTAAAACAAATTAGGGAAATAGAGAAGGAAATCGGGAAGGTAATGGTTGAAGATGATCTC
CTGACGGAGGTAGTTGATTTAGTAGAATACCCTACTGCATTTTACGGTAAATTTGATAGGTCTTATCTGGAACTTCCCGA
TGATGTATTAATAACTTCAATGGCCGAGCATCAAAGATATTTCCCTGTAATTGATGAAGATGGATCCCTCAGTCCTTACT
TCGTCGGGGTCAGGGATGGGATAGAAGATTATATTGAAGAAGTGAGATATGGAAATGAAATGGTACTCAGGGCCCGCCTG
GCAGATGCCCGTTTCTTTTTTGAAGAAGACCTTAAAGTTAGTATCGAAGAACGGCAAAAGGAACTTGAGGAAATTGTTTT
CCAGGAAGACCTTGGTTCAATGATGGATAAAGTAAAGAGATTAAAACAACTGGTTATTCAGATTGGAAAATCATTAAATC
TGAAAGAAAGCCAGTTACAGAGCCTTATTAGGGCAGCTGAACTCAGCAAAAATGACCTTGTAACTGAAATGGTTAATGAA
TTTACCAAATTACAGGGAGTAATGGGGCGTGAATATGCTCTGATTAATGGTGAGGATGAAGAGGTTGCTACTGCAATATA
TGAACAGTATCTTCCCCGATATTCCGGTGATAGACTACCCCAGACTTTATATGGTCGTATTCTGAGTATAGCTGATAAAA
TAGATAATATTACCAGTCATTTCAGTCTCGGGATGATACCCAGTGGCTCCCAGGATCCTTTTGCCTTGAGACGTCAGGCT
AATGGGATTGTGAATATTATTATAGATGCTCAACTTCCCCTTAAATTATCTTCTCTGTTGAACTGGAGTATAGAGGTGTT
AGAGCCTGGAGATAAAGATTTTGTGAATGAGGAAAAAAGTTTTCTGCTTCAGAGGTTAGAAACCATTCTCGATGAACGTG
GTATCAGGTATGATATTATTAATTCAGTCGTGAGAGTAGGTGATGATGACCCCAATAATATTTTATCCAGAGCTGAAGCA
GTTATGTCCTTAAGGAAGGAAAACCCTGATTTATTTGTTGACTTAATTCAGGGTCTCGTCAGAGCAAGGAATCTGGCTTC
AAAAGGTGAAGGCAATAATGATATAAACCCTGAATACTTTGAATGCAGGGAAGAAAAAGAACTTTATGATATATATCGCA
AAATTAAAGATGAAATTCAAAGACAATTTAAGAAAAAAAATTACCTTTCAGGTTTAAAAAAGCTGGTAGATGTAAAGGAA
CCGGTAGATAATTTTCTTGATAATGTAGTTGTAATGGTTGAAGATGAGAGGATTAAGAATAATCGGCTGGCTTTACTCCA
GGAAATCAGTAATCTGGTAAGTGGGGTTATGAATATAAGTGAGATTGCTCTGGATGATTAA

Upstream 100 bases:

>100_bases
GGGTACAGGTATATATTTTAGTATACAGGTATAAAAAATTTAAAATAAATCCTGGAAGTGTAACCGGACTAAAGACTAAA
TTATAAAAGGAGTGATGGAG

Downstream 100 bases:

>100_bases
TTAATATAGAGGTGGTATGATGACAGAAAAACCCCGAATTTTTATTGTATCTGACTCTATCGGTGAGACTGCCCAGTATG
TTGTAGATGCAACTGTAAGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MARDLLFEIGTEEMPAGLIGKIRSDLKDLAINTLEDKRLDFKECKVFSTPRRLVLFVKELAEKQEEKREVVKGPARSIAF
EDDGTPTRAAKGFSRAQGVEVDDLIIKDDYVYVEKIEQGQDTAGLLKDILPGLINKLPLPRSMRWADYNFKFIRPIRWLL
ALFGEEIISFSMAGVQSGAYTRGHRFLVKDRLEVKDPDHYFDVMEKGYIIVDHNKRRELILKQIREIEKEIGKVMVEDDL
LTEVVDLVEYPTAFYGKFDRSYLELPDDVLITSMAEHQRYFPVIDEDGSLSPYFVGVRDGIEDYIEEVRYGNEMVLRARL
ADARFFFEEDLKVSIEERQKELEEIVFQEDLGSMMDKVKRLKQLVIQIGKSLNLKESQLQSLIRAAELSKNDLVTEMVNE
FTKLQGVMGREYALINGEDEEVATAIYEQYLPRYSGDRLPQTLYGRILSIADKIDNITSHFSLGMIPSGSQDPFALRRQA
NGIVNIIIDAQLPLKLSSLLNWSIEVLEPGDKDFVNEEKSFLLQRLETILDERGIRYDIINSVVRVGDDDPNNILSRAEA
VMSLRKENPDLFVDLIQGLVRARNLASKGEGNNDINPEYFECREEKELYDIYRKIKDEIQRQFKKKNYLSGLKKLVDVKE
PVDNFLDNVVVMVEDERIKNNRLALLQEISNLVSGVMNISEIALDD

Sequences:

>Translated_686_residues
MARDLLFEIGTEEMPAGLIGKIRSDLKDLAINTLEDKRLDFKECKVFSTPRRLVLFVKELAEKQEEKREVVKGPARSIAF
EDDGTPTRAAKGFSRAQGVEVDDLIIKDDYVYVEKIEQGQDTAGLLKDILPGLINKLPLPRSMRWADYNFKFIRPIRWLL
ALFGEEIISFSMAGVQSGAYTRGHRFLVKDRLEVKDPDHYFDVMEKGYIIVDHNKRRELILKQIREIEKEIGKVMVEDDL
LTEVVDLVEYPTAFYGKFDRSYLELPDDVLITSMAEHQRYFPVIDEDGSLSPYFVGVRDGIEDYIEEVRYGNEMVLRARL
ADARFFFEEDLKVSIEERQKELEEIVFQEDLGSMMDKVKRLKQLVIQIGKSLNLKESQLQSLIRAAELSKNDLVTEMVNE
FTKLQGVMGREYALINGEDEEVATAIYEQYLPRYSGDRLPQTLYGRILSIADKIDNITSHFSLGMIPSGSQDPFALRRQA
NGIVNIIIDAQLPLKLSSLLNWSIEVLEPGDKDFVNEEKSFLLQRLETILDERGIRYDIINSVVRVGDDDPNNILSRAEA
VMSLRKENPDLFVDLIQGLVRARNLASKGEGNNDINPEYFECREEKELYDIYRKIKDEIQRQFKKKNYLSGLKKLVDVKE
PVDNFLDNVVVMVEDERIKNNRLALLQEISNLVSGVMNISEIALDD
>Mature_685_residues
ARDLLFEIGTEEMPAGLIGKIRSDLKDLAINTLEDKRLDFKECKVFSTPRRLVLFVKELAEKQEEKREVVKGPARSIAFE
DDGTPTRAAKGFSRAQGVEVDDLIIKDDYVYVEKIEQGQDTAGLLKDILPGLINKLPLPRSMRWADYNFKFIRPIRWLLA
LFGEEIISFSMAGVQSGAYTRGHRFLVKDRLEVKDPDHYFDVMEKGYIIVDHNKRRELILKQIREIEKEIGKVMVEDDLL
TEVVDLVEYPTAFYGKFDRSYLELPDDVLITSMAEHQRYFPVIDEDGSLSPYFVGVRDGIEDYIEEVRYGNEMVLRARLA
DARFFFEEDLKVSIEERQKELEEIVFQEDLGSMMDKVKRLKQLVIQIGKSLNLKESQLQSLIRAAELSKNDLVTEMVNEF
TKLQGVMGREYALINGEDEEVATAIYEQYLPRYSGDRLPQTLYGRILSIADKIDNITSHFSLGMIPSGSQDPFALRRQAN
GIVNIIIDAQLPLKLSSLLNWSIEVLEPGDKDFVNEEKSFLLQRLETILDERGIRYDIINSVVRVGDDDPNNILSRAEAV
MSLRKENPDLFVDLIQGLVRARNLASKGEGNNDINPEYFECREEKELYDIYRKIKDEIQRQFKKKNYLSGLKKLVDVKEP
VDNFLDNVVVMVEDERIKNNRLALLQEISNLVSGVMNISEIALDD

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=693, Percent_Identity=34.0548340548341, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_HALOH (B8CXH5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508989.1
- ProteinModelPortal:   B8CXH5
- GeneID:   7313565
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_12440
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 78941; Mature: 78810

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLLFEIGTEEMPAGLIGKIRSDLKDLAINTLEDKRLDFKECKVFSTPRRLVLFVKEL
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
AEKQEEKREVVKGPARSIAFEDDGTPTRAAKGFSRAQGVEVDDLIIKDDYVYVEKIEQGQ
HHHHHHHHHHHCCCHHEEEECCCCCCHHHHHCHHHHCCCCHHHEEEECCCCEEHHHHCCC
DTAGLLKDILPGLINKLPLPRSMRWADYNFKFIRPIRWLLALFGEEIISFSMAGVQSGAY
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRGHRFLVKDRLEVKDPDHYFDVMEKGYIIVDHNKRRELILKQIREIEKEIGKVMVEDDL
CCCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTEVVDLVEYPTAFYGKFDRSYLELPDDVLITSMAEHQRYFPVIDEDGSLSPYFVGVRDG
HHHHHHHHHCCHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEHHHH
IEDYIEEVRYGNEMVLRARLADARFFFEEDLKVSIEERQKELEEIVFQEDLGSMMDKVKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQLVIQIGKSLNLKESQLQSLIRAAELSKNDLVTEMVNEFTKLQGVMGREYALINGEDE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
EVATAIYEQYLPRYSGDRLPQTLYGRILSIADKIDNITSHFSLGMIPSGSQDPFALRRQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHC
NGIVNIIIDAQLPLKLSSLLNWSIEVLEPGDKDFVNEEKSFLLQRLETILDERGIRYDII
CCEEEEEEECCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NSVVRVGDDDPNNILSRAEAVMSLRKENPDLFVDLIQGLVRARNLASKGEGNNDINPEYF
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ECREEKELYDIYRKIKDEIQRQFKKKNYLSGLKKLVDVKEPVDNFLDNVVVMVEDERIKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHCC
NRLALLQEISNLVSGVMNISEIALDD
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARDLLFEIGTEEMPAGLIGKIRSDLKDLAINTLEDKRLDFKECKVFSTPRRLVLFVKEL
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHH
AEKQEEKREVVKGPARSIAFEDDGTPTRAAKGFSRAQGVEVDDLIIKDDYVYVEKIEQGQ
HHHHHHHHHHHCCCHHEEEECCCCCCHHHHHCHHHHCCCCHHHEEEECCCCEEHHHHCCC
DTAGLLKDILPGLINKLPLPRSMRWADYNFKFIRPIRWLLALFGEEIISFSMAGVQSGAY
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TRGHRFLVKDRLEVKDPDHYFDVMEKGYIIVDHNKRRELILKQIREIEKEIGKVMVEDDL
CCCCEEEEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTEVVDLVEYPTAFYGKFDRSYLELPDDVLITSMAEHQRYFPVIDEDGSLSPYFVGVRDG
HHHHHHHHHCCHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEHHHH
IEDYIEEVRYGNEMVLRARLADARFFFEEDLKVSIEERQKELEEIVFQEDLGSMMDKVKR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQLVIQIGKSLNLKESQLQSLIRAAELSKNDLVTEMVNEFTKLQGVMGREYALINGEDE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
EVATAIYEQYLPRYSGDRLPQTLYGRILSIADKIDNITSHFSLGMIPSGSQDPFALRRQA
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHC
NGIVNIIIDAQLPLKLSSLLNWSIEVLEPGDKDFVNEEKSFLLQRLETILDERGIRYDII
CCEEEEEEECCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NSVVRVGDDDPNNILSRAEAVMSLRKENPDLFVDLIQGLVRARNLASKGEGNNDINPEYF
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ECREEKELYDIYRKIKDEIQRQFKKKNYLSGLKKLVDVKEPVDNFLDNVVVMVEDERIKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHCC
NRLALLQEISNLVSGVMNISEIALDD
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA